ABSTRACT
Sepsis-associated encephalopathy (SAE) is a severe complication that affects the central nervous system and is a leading cause of increased morbidity and mortality in intensive care units. Psoralidin (PSO), a coumarin compound isolated from the traditional Chinese medicine Psoralea corylifolia L., can penetrate the blood-brain barrier and has various pharmacological activities, including anti-inflammation, anti-oxidation and anti-depression. This study aims to explore whether PSO alleviates SAE and delve into the underlying mechanisms. We found that PSO treatment significantly reduced sepsis scores, aspartate transaminase (AST) and aspartate transaminase (LDH), while increased anal temperature and neurological scores in CLP-injured mice. Moreover, PSO treatment ameliorated sepsis-associated cognitive impairment, mood, anxiety disorders, inhibited inflammatory responses, as well as attenuated endoplasmic reticulum stress (ERS). These results were also validated in vitro experiments, PSO treatment reduced ROS, inflammation response, and attenuated ERS in LPS-injured N2a cells. Importantly, tunicamycin (TUN), as ERS agonist, significantly reversed the protective effect of PSO on LPS-injured N2a cells, as evidenced by increased expression levels of IL-6, NLRP3, CHOP, and ATF6. Likewise, ATF6 overexpression also reversed the protective effect of PSO. In conclusion, these results confirmed that PSO has a protective effect on SAE, which was largely attributed to neuroinflammation and ERS. These findings provide new insights into the neuroprotective role of PSO and suggest that PSO is a new therapeutic intervention of SAE.
Subject(s)
Benzofurans , Coumarins , Endoplasmic Reticulum Stress , Sepsis-Associated Encephalopathy , Animals , Endoplasmic Reticulum Stress/drug effects , Mice , Coumarins/pharmacology , Sepsis-Associated Encephalopathy/drug therapy , Sepsis-Associated Encephalopathy/metabolism , Sepsis-Associated Encephalopathy/pathology , Benzofurans/pharmacology , Male , Lipopolysaccharides/toxicity , Sepsis/drug therapy , Sepsis/complications , Sepsis/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein/genetics , Disease Models, Animal , Reactive Oxygen Species/metabolism , Tunicamycin/pharmacology , Mice, Inbred C57BLABSTRACT
Bifunctional photocatalytic nanofiltration (PNF) membrane is increasingly concerned in practical micro-polluted water purification, but there are still several bottlenecks that inhibit its practicality. In this context, the feasibility of a novel metal-free and visible light-responsive surface-anchored PNF membrane for simultaneously removing target antibiotics in real sewage effluent in a continuous dynamic process was explored. The results showed that the optimal PNF-4 membrane was expectedly consisted of an inside tight sub-nanopore structured separation layer and an outside thinner, smoother, super hydrophilic mesoporous degradation layer, respectively. Consequently, the activated PNF-4 membrane could synergistically reduce trimethoprim and sulfamethoxazole concentrations to below two orders of magnitude, accompanying with almost constant high water permeability, suggesting that the hydrophilic modification of the mesoporous degradation layer basically offsets its inherent hydraulic resistance. Also, after repeating the fouling-physical rinsing process three times lasted for 78 h, only sporadic adherent contaminants remained onto the top surface, together with the minimal total and irreversible fouling ratios (as low as 7.2% and 1.2%, respectively), strongly demonstrated that PNF-4 membrane displayed good self-cleaning performance. Undoubtedly, this will significantly reduce its potential cleaning frequency and maintenance cost in long-term operation. Meanwhile, the acute and chronic biotoxicities of its permeate to Virbrio qinghaiensis sp. -67 were also reduced sharply to 2.22% and 0.45%, respectively. All of these evidences suggest that the dual functions of PNF-4 membrane are synergetic in an uninterrupted permeating process. It will provide useful insights for continuously enhancing the practicality and effectiveness of PNF membrane in actual micro-polluted water purification scenarios.
Subject(s)
Anti-Bacterial Agents , Water Purification , Sewage , Light , Sulfamethoxazole , Trimethoprim , Membranes, Artificial , Water Purification/methodsABSTRACT
Rare information is available on fouling behavior of customized nanofiltration (NF) membrane evoked by pharmaceutically active compounds (PhACs) under real multiple influent matrices pretreated by ultrafiltration module beforehand. To this end, a novel tight NF membrane with excellent perm-selectivity and antiadhesion was fabricated and used to assess its separation performance/mechanism and fouling behavior to a broad range of small molecular PhACs in the context. The adsorption ratio results revealed that the affinities between five selected PhACs and the customized nanocomposite membrane surface were all much weaker (below 5.5%) than the solute-solute interacting forces (between 23.6 and 83.2%), whether for natural or synthetic complex micropollutants. The predominant membrane fouling could be interpreted by the incomplete blocking model in the permeation of both influent conditions. For neat nanocomposite membrane, the order of critical factors important on separation mechanism was electrostatic effect, adsorption and steric hindrance. The fouling layer seemed to act as a secondary separating layer for those negatively charged or hydrophilic PhACs, but showed the cake enhanced concentration polarization effect for the neutral and hydrophobic ones. This study provides valuable insights for defining PhACs fate and NF membrane fouling behavior to fit increasingly stringent criteria for wastewater treatment.
Subject(s)
Nanocomposites , Water Purification , Adsorption , Membranes, Artificial , UltrafiltrationABSTRACT
As a new kind of green solvents for potential replacement of traditional volatile organic compounds, deep eutectic solvents (DESs) have been attracting more and more attention in various applications. In this work, three types of hydrophobic DESs were synthesized by simple mixing of trioctylmethyl ammonium chloride (used as hydrogen bond acceptor) with decanoic acid, ketoprofen and gemfibrozil (hydrogen bond donors), respectively, at different molar ratios. In order to evaluate the solvent characteristics of these DESs, some of the physical properties such as melting point, density, viscosity, and water contact angle were determined. Then, these hydrophobic DESs were used for the vortex-assisted liquid-liquid microextraction of bisphenol-type contaminants in water, followed by quantitative determination of bisphenols contents with high performance liquid chromatography (HPLC-FLD) coupled with fluorescence detection. For this purpose, four bisphenols, namely, 2, 2-bis(4-hydroxydiphenyl)propane (BPA), 2, 2-bis (4-hydroxyphenyl)butane (BPB), 4, 4'-(1-phenylethylidene)bisphenol (BPAP) and 4, 4'-cyclohexylidenebisphenol (BPZ) were selected as model contaminants. It was found that the enrichment factors of the proposed method were in the range from 97 to 112, depending on the structure of the analytes. Under optimal experimental conditions, the linearity ranges of the method varied from 0.3 to 700 µg L-1 with linear correlation coefficients (R2) higher than 0.996. The limit of detections (LODs) and limit of quantifications (LOQs) were in the range of 0.3-0.5 µg L-1 and 0.06-0.08 µg L-1, respectively. Furthermore, the developed method was successfully used in the extraction and determination of four bisphenols from food-contacted plastic samples.
Subject(s)
Benzhydryl Compounds/analysis , Chromatography, High Pressure Liquid/methods , Food , Hydrophobic and Hydrophilic Interactions , Phenols/analysis , Plastics/chemistry , Solvents/chemistry , Benzhydryl Compounds/urine , Fluorescence , Humans , Hydrogen Bonding , Hydrogen-Ion Concentration , Limit of Detection , Liquid Phase Microextraction , Phenols/urine , Reproducibility of Results , Solutions , Spectroscopy, Fourier Transform Infrared , Time Factors , Water Pollutants, Chemical/analysisABSTRACT
In this work, a novel class of eight low viscosity hydrophobic deep eutectic solvents (DESs) has been designed and prepared from two kinds of quaternary ammonium salts and six kinds of water-insoluble fatty alcohols/acids. Several physical properties of these DESs, such as melting point, density and viscosity, have been determined to investigate their adaptability as solvents. Then, such DESs are used as extraction solvents in a vortex-assisted liquid-liquid microextraction for the extraction and preconcentration of phthalate esters (PEs) from water samples, and the content of PEs from food-contacted plastics is then determined by using gas chromatography. It is found that the analytic method developed in this work exhibits wide linear range of 5-1000⯵gâ¯L-1, and the limit of detections for target analytes is as low as 1⯵gâ¯L-1. Finally, the proposed method has been successfully used for the determination of PEs from food-contacted plastics.
Subject(s)
Chromatography, Gas , Liquid Phase Microextraction/methods , Phthalic Acids/analysis , Plastics/chemistry , Esters/chemistry , Food Packaging , Hydrogen-Ion Concentration , Hydrophobic and Hydrophilic Interactions , Limit of Detection , Phthalic Acids/isolation & purification , Reproducibility of Results , Solvents/chemistryABSTRACT
This paper aims to validate the feasibility of hairwork dyeing effluent (HDE) reclamation using an ultrafiltration (UF)-reverse osmosis (RO) integrated membrane system combined with coagulation-flocculation and sedimentation acquiring the highest possible product water recovery rate along with both satisfactory separation performance and well controlled membrane fouling. Under the circumstance of only physical cleaning involved, the laboratory-scale test yielded a higher and satisfactory reuse ratio of 76% for HDE, and the corresponding RO product as reclaimed water contained only 223â¯mg·L-1 of TDS, 3.87â¯mg·mL-1 of DOC and 10.3â¯mg·mL-1 of total hardness, which was obviously better than the quality of existing feedwater in hairwork dyeing process. After each processing unit, the distributions of fulvic (region III) and humic (region V) organics decreased continuously, while an overall rising trend in distribution of protein-like organics (regions I and II) was observed. Contact angle for the fouled UF and RO membranes significantly increased by 19.5° and decreased by 19.7°, respectively, which suggested that different polarity of organic or inorganic adsorption rather than membrane roughness was the main factors affecting wetting properties of the fouled employed membranes. Both ATR-FTIR and XPS spectra indicated that organic fouling on UF membrane surface under harsh condition (RUFâ¯=â¯90%) was mild and tolerable, whereas a surprising amount of hydrophilic micromolecular organics riched in carboxyl and hydroxyl functional groups were absorbed on RO membrane surface after permeation.
ABSTRACT
As a new type of green solvents, deep eutectic solvents (DESs) are attracting more and more attentions due to their unique properties and cheapness. However, most DESs reported previously were hydrophilic and thus limiting their applications in aqueous medium. In this study, a novel hydrophobic DES was prepared by mixing ethylparaben and methyl trioctyl ammonium chloride, and density, viscosity, and contact angle of the DES were determined to character its solvent characteristics. Then, this DES was used as an efficient additive of sol-gel sorbent coating of poly(dimethylsiloxane) (PDMS) fiber. It was found that the addition of hydrophobic DES could generate a lot of neat pores in the PDMS fiber surface, thus significantly improving the performance of PDMS fiber coating. The feasibility of PDMS-DES adopted for head space solid phase microextraction was evaluated by GC-FID for the extraction and determination of volatile organic compounds, such as toluene, ethylbenzene and o-xylene. Key parameters affecting the extraction efficiency were investigated systematically. Under optimal conditions, the linear range with PDMS-DES fiber lied between 10 and 1000⯵gâ¯L-1, and the limit of detection (LOD) was in the range from 0.005 to 0.025⯵gâ¯L-1. Compared with homemade PDMS fiber, the peak area of signal response for the PDMS-DES fiber was 3 times higher. At the same time, the LOD for the proposed method was much lower than the commercial 100⯵m-PDMS fiber coating.