The effects of desferrioxamin and vitamin E as supplements to antibiotics in the treatment of peritonitis in rats.

AIM: The aim of the study was to determine the effects of vitamin E and the iron chelating agent desferrioxamin (Dfx), supplemented by clindamycin and gentamycin therapy, on peritonitis caused by caecal ligation of a puncture wound in an experimental model. MATERIALS AND METHODS: One hundred and twenty Spraque Dawley rats were divided into eight groups. Three groups were used as controls; intraperitoneal (i.p.), subcutaneous (s.c.) and i.p. and s.c., respectively. Group 4 was treated with Dfx, Group 5 with vitamin E and Group 6 with antibiotics. Group 7 was treated with vitamin E in combination with antibiotics, and Group 8 with a combination of antibiotics and Dfx. The rats were studied for 14 days following treatment, and survivors then humanely dispatched. Post-mortem examination was undertaken on all the rats studied. RESULTS: In the control groups, mortality at 14 days was 66%. Rats treated with antibiotics alone (Group 5) had a mortality rate of 40%. Those treated with a combination of antibiotics and vitamin E (Group 7), however, had a mortality rate of only 14%, and those treated with antibiotics and Dfx had a mortality rate of only 7%. CONCLUSION: This study suggests that treatment of peritonitis in rats with a combination of Dfx and antibiotics has a significant beneficial effect on survival, in comparison with treatment with antibiotics alone.

Gastric tonometer monitoring in infants undergoing repair of coarctation of the aorta.

OBJECTIVE: To evaluate gastric tonometer monitoring for splanchnic hypoperfusion in infants during surgical intervention for aortic coarctation, especially within aortic cross-clamp periods. DESIGN: A prospective study. SETTING: Cardiovascular intensive care unit in a university hospital. PARTICIPANTS: Fourteen infant patients after elective, uncomplicated repair of coarctation of the aorta. INTERVENTIONS: After the anesthesia induction, a 7F tonometry catheter was inserted into the stomach oropharyngeally. Gastric carbon dioxide, arterial blood gases, blood pressure of upper extremities, and hematocrit values were measured in 5 different time intervals. Time periods were as follows: T1 (after the anesthesia induction), T2 (before aortic cross-clamp), T3 (immediately after aortic cross-clamp removal), T4 (40 minutes after aortic cross-clamp removal), and T5 (as the patient reached the intensive care unit). Intramucosal pH was measured by means of the Henderson-Hasselbach equation. The mean values of all parameters were calculated. According to T1 time, T2, T3, T4, and T5 times were compared with Student's t-test. MEASUREMENTS AND MAIN RESULTS: Mean aortic cross-clamp time was 19.4 +/- 6.6 minutes. Intramucosal pH values of T3 (p < 0.001) and T4 (p < 0.01) were found to be lower than values of T1. The gastric carbon dioxide values of T3 were significantly higher than T1 (p < 0.01), and bicarbonate and arterial pH values of T3 were significantly lower (p < 0.01). There were no significant differences in other parameters over time intervals. CONCLUSION: Splanchnic hypoperfusion exists during aortic cross-clamping in infant aortic coarctation surgery, and the tonometric catheter is considered to be a safe method for monitoring this hypoperfusion.

Effects of phentolamine on tissue perfusion in pediatric cardiac surgery.

OBJECTIVE: To evaluate whether the deleterious effects of cardiopulmonary bypass (CPB) can be overcome by phentolamine-induced pharmacologic vasodilation in pediatric patients with congenital heart disease. DESIGN: Prospective, randomized, clinical study. SETTING: Single university hospital. PARTICIPANTS: Forty-three pediatric patients undergoing open cardiac surgery for repair of congenital heart disease. INTERVENTIONS: Patients were randomly allocated into two groups. Patients in group 1 (n = 22) received 0.2 mg/kg of phentolamine during the cooling and rewarming periods of CPB. Group 2 patients (n = 21) did not receive phentolamine. Temperature measurements (rectal [R], nasopharyngeal [N], and toe [P]) and serum lactate values were obtained before, during, and after CPB; systemic oxygen consumption was evaluated during CPB. MEASUREMENTS AND MAIN RESULTS: At the end of the CPB period and at the end of the operation, lactate values of group 1 (1.87+/-0.37 and 1.8+/-0.39 mmol/L, respectively) were significantly lower than values of group 2 (2.24+/-0.28 and 2.33+/-0.33 mmol/L; p < 0.05 and p < 0.05, respectively). At the beginning of the rewarming period N-R temperature gradients of group 1 (0.14 degrees C+/-0.92 degrees C) were lower than group 2 (-0.58 degrees C+/-1.84 degrees C) values (p < 0.05). Central-peripheral temperature gradients of group 1 obtained at the end of the CPB period (N-R = 2.18 degrees C+/-0.69 degrees C; N-P = 7.84 degrees C+/-1.54 degrees C; R-P = 5.66 degrees C+/-1.70 degrees C) were significantly lower than the values of group 2 (N-R = 2.80 degrees C+/-0.91 degrees C, N-P = 9.97 degrees C+/-2.02 degrees C; R-P = 7.18 degrees C+/-2.10 degrees C; p < 0.05; p < 0.001; p < 0.05). At the end of the operation values of group 1 (N-R = 0.48 degrees C+/-0.31 degrees C; N-P = 6.30 degrees C+/-1.23 degrees C; R-P = 5.82 degrees C+/-1.16 degrees C) were significantly lower than the values of group 2 (N-R = 0.94 degrees C+/-0.56 degrees C; N-P = 8.69 degrees C+/-0.28 degrees C; R-P = 7.75 degrees C+/-2.15 degrees C; p < 0.05; p < 0.001; p < 0.001). The systemic oxygen consumption values of group 1 were higher than group 2 (6.26+/-1.82 v 5.17+/-1.05 mL/min/kg; p < 0.05) after complete rewarming. Mean arterial pressure (MAP) values of group 1 (58.9+/-6.4 mmHg) were lower than group 2 (63.4+/-6.7 mmHg) at the period after CPB (p = 0.03). CONCLUSION: The results suggest that the use of phentolamine during CPB is associated with limited systemic anaerobic metabolism and more uniform body perfusion.