ABSTRACT
Primordial germ cells (PGCs), the precursors of sperm and eggs, are the route to totipotency and require establishment of a unique epigenome in this lineage. The genetic program for PGC specification in the mouse also initiates epigenetic reprogramming that continues when PGCs migrate into the developing gonads. Among these later events is active and genome-wide DNA demethylation, which is linked to extensive chromatin remodeling. These extensive epigenetic changes erase most, if not all, of the existing epigenetic information, which resets the epigenome for totipotency. Recent evidence suggests that active DNA demethylation involves a base excision repair (BER) pathway. BER is mechanistically linked to DNA demethylation, but what triggers BER is currently under investigation. The methylated cytosine (5mC) could be modified by deamination or to 5hmC, which could induce BER. Detection of Tet1 expression specifically and coincidentally, at the time of BER in PGCs, suggests that conversion of 5mC to 5hmC might be involved, at least in part, during epigenetic reprogramming and DNA demethylation in germ cells.
Subject(s)
Cellular Reprogramming/genetics , Epigenesis, Genetic , Germ Cells/metabolism , Totipotent Stem Cells/metabolism , Animals , DNA Breaks , DNA Methylation/genetics , DNA Repair/genetics , MiceABSTRACT
The germ cell lineage has the unique attribute of generating the totipotent state. Development of blastocysts from the totipotent zygote results in the establishment of pluripotent primitive ectoderm cells in the inner cell mass of blastocysts, which subsequently develop into epiblast cells in postimplantation embryos. The germ cell lineage in mice originates from these pluripotent epiblast cells of postimplantation embryos in response to specific signals. Pluripotent stem cells and unipotent germ cells share some fundamental properties despite significant phenotypic differences between them. Additionally, early primordial germ cells can be induced to undergo dedifferentiation into pluripotent embryonic germ cells. Investigations on the relationship between germ cells and pluripotent stem cells may further elucidate the nature of the pluripotent state. Furthermore, comprehensive epigenetic reprogramming of the genome in early germ cells, including extensive erasure of epigenetic modifications, is a critical step toward establishment of totipotency. The mechanisms involved may be relevant for gaining insight into events that lead to reprogramming of somatic cells into pluripotent stem cells.
Subject(s)
Epigenesis, Genetic , Germ Cells/cytology , Germ Cells/metabolism , Totipotent Stem Cells/cytology , Totipotent Stem Cells/metabolism , Animals , Embryonic Stem Cells/cytology , Embryonic Stem Cells/metabolism , Female , Mice , Models, Genetic , Multipotent Stem Cells/cytology , Multipotent Stem Cells/metabolism , Mutation , Pregnancy , Protein Methyltransferases/genetics , Protein Methyltransferases/metabolism , Protein-Arginine N-MethyltransferasesABSTRACT
Germ cells provide an enduring link between generations and therefore must possess the fundamental ability of reprogramming their genome to generate a totipotent state. We wish to understand the molecular basis of the unique properties of the mammalian germ line. Recently we identified Blimp1, a potent transcriptional repressor of a histone methyltransferase subfamily, as a critical determinant of the germ cell lineage in mice. Surprisingly, Blimp1 expression marks the origin of the germ line in proximal epiblast cells in pregastrulation embryos, substantially earlier than previously thought. Furthermore, we showed that established primordial germ cells undergo extensive erasure of genome-wide histone H3 lysine 9 dimethylation (H3K9me2) and DNA methylation, two major repressive epigenetic modifications, and instead acquire high levels of H3-K27 trimethylation (H3K27me3) in their migration period. We suggest that germline specification is a genetic system for the orderly reprogramming of the cells' epigenome toward a totipotent state, with reacquisition of totipotency-associated transcription factors and continued Blimp1 expression preventing their reversion to an explicit pluripotent state or somatic differentiation.
Subject(s)
Epigenesis, Genetic/genetics , Gene Expression Regulation, Developmental , Germ Cells/cytology , Germ Cells/metabolism , Animals , Cell Lineage , Humans , Mice , Time Factors , Transcription Factors/metabolismABSTRACT
The discovery of the phenomenon of genomic imprinting in mammals showed that the parental genomes are functionally non-equivalent. Considerable advances have occurred in the field over the past 20 years, which has resulted in the identification and functional analysis of a number of imprinted genes the expression of which is determined by their parental origin. These genes belong to many diverse categories and they have been shown to regulate growth, complex aspects of mammalian physiology and behavior. Many aspects of the mechanism of imprinting have also been elucidated. However, the reasons for the evolution of genomic imprinting remain enigmatic. Further research is needed to determine if there is any relationship between the apparently diverse functions of imprinted genes in mammals, and their role in human diseases. It also remains to be seen what common features exist amongst the diverse imprinting control elements. The mechanisms involved in the erasure and re-establishment of imprints should provide deeper insights into epigenetic mechanisms of wide general interest.
Subject(s)
Genomic Imprinting , Mammals/genetics , Animals , Embryonic Development , Female , Male , Mammals/metabolism , Mammals/psychology , ReproductionABSTRACT
Genomic imprinting at the H19/Igf2 locus is governed by a cis-acting Imprinting-Control Region (ICR), located 2 kb upstream of the H19 gene. This region possesses an insulator function which is activated on the unmethylated maternal allele through the binding of the CTCF factor. It has been previously reported that paternal transmission of the H19(SilK) deletion, which removes the 3' portion of H19 ICR, leads to the loss of H19 imprinting. Here we show that, in the liver, this reactivation of the paternal H19 gene is concomitant to a dramatic decrease in Igf2 mRNA levels. This deletion alters higher-order chromatin architecture, Igf2 promoter usage and tissue-specific expression. Therefore, when methylated, the 3' portion of the H19 ICR is a bi-functional regulatory element involved not only in H19 imprinting but also in 'formatting' the higher-order chromatin structure for proper tissue-specific expression of both H19 and Igf2 genes.
Subject(s)
Gene Expression Regulation , Insulin-Like Growth Factor II/genetics , RNA, Untranslated/genetics , Animals , Crosses, Genetic , Female , Genomic Imprinting , Heart/physiology , Liver/physiology , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Organ Specificity , RNA, Long Noncoding , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Peg3 encodes a C2H2 type zinc finger protein that is implicated in a novel physiological pathway regulating core body temperature, feeding behavior, and obesity in mice. Peg3+/- mutant mice develop an excess of abdominal, subcutaneous, and intra-scapular fat, despite a lifetime of lower food intake than wild-type animals. However, they start life with reduced fat reserves and are slower to enter puberty. These mice maintain a lower core body temperature, fail to respond to a cold challenge, and have lower metabolic activity as measured by oxygen consumption. Plasma leptin levels are significantly higher than in wild types, and Peg3+/- mice appear to have developed leptin resistance. Administration of exogenous leptin resulted in a significant reduction in food intake in wild-type mice that was not observed in Peg3+/- mutants. This mutation, which is strongly expressed in hypothalamic tissue during development, has the capacity to regulate multiple events relating to energy homeostasis.
Subject(s)
Adipose Tissue/metabolism , Protein Kinases/genetics , Protein Kinases/physiology , Transcription Factors/genetics , Transcription Factors/physiology , Animals , Body Composition , Body Temperature , Body Weight , Eating/drug effects , Energy Metabolism , Female , Hypothalamus/physiology , Kruppel-Like Transcription Factors , Leptin/blood , Leptin/pharmacology , Male , Mice , Motor Activity , Mutation , Neuropeptide Y/genetics , Obesity/etiology , Oxygen Consumption , Pro-Opiomelanocortin/genetics , RNA, Messenger/analysis , Sexual MaturationSubject(s)
Epigenesis, Genetic , Germ Cells/cytology , Animals , Caenorhabditis elegans/cytology , Caenorhabditis elegans/genetics , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Female , Male , Mice , Models, Genetic , Phenotype , Positive Regulatory Domain I-Binding Factor 1 , Repressor Proteins/genetics , Repressor Proteins/metabolism , Totipotent Stem Cells/cytology , Transcription Factors/genetics , Transcription Factors/metabolismABSTRACT
BACKGROUND: Specification of primordial germ cells in mice depends on instructive signalling events, which act first to confer germ cell competence on epiblast cells, and second, to impose a germ cell fate upon competent precursors. fragilis, an interferon-inducible gene coding for a transmembrane protein, is the first gene to be implicated in the acquisition of germ cell competence. RESULTS: Here, we describe four additional fragilis-related genes, fragilis2-5, which are clustered within a 68 kb region in the vicinity of the fragilis locus on Chr 7. These genes exist in a number of mammalian species, which in the human are also clustered on the syntenic region on Chr 11. In the mouse, fragilis2 and fragilis3, which are proximate to fragilis, exhibit expression that overlaps with the latter in the region of specification of primordial germ cells. Using single cell analysis, we confirm that all these three fragilis-related genes are predominant in nascent primordial germ cells, as well as in gonadal germ cells. CONCLUSION: The Fragilis family of interferon-inducible genes is tightly associated with germ cell specification in mice. Furthermore, its evolutionary conservation suggests that it probably plays a critical role in all mammals. Detailed analysis of these genes may also elucidate the role of interferons as signalling molecules during development.
Subject(s)
Germ Cells/physiology , Membrane Proteins/physiology , Sequence Alignment , Amino Acid Sequence , Animals , Blastomeres/chemistry , Blastomeres/cytology , Blastomeres/physiology , Cattle , Embryo, Mammalian , Embryonic Development/physiology , Female , Germ Cells/chemistry , Germ Cells/growth & development , Humans , Membrane Proteins/biosynthesis , Membrane Proteins/genetics , Mice , Molecular Sequence Data , Organ Specificity/genetics , Organ Specificity/physiology , Pregnancy , RatsSubject(s)
Fertilization/physiology , Oocytes/physiology , Spermatozoa/physiology , Animals , DNA (Cytosine-5-)-Methyltransferases/genetics , DNA (Cytosine-5-)-Methyltransferases/physiology , DNA Methyltransferase 3A , Embryonic and Fetal Development/genetics , Embryonic and Fetal Development/physiology , Female , Genome , Genomic Imprinting , Humans , Male , Mutation , DNA Methyltransferase 3BABSTRACT
In the normal diploid mouse embryo, active demethylation of the paternal genome but not of the maternal genome occurs within only a few hours and in a highly coordinated fashion as the zygote proceeds through the first G1 phase. This zygotic demethylation may be necessary to reprogram the sperm genome for somatic development. Immunofluorescence staining with an antibody against 5-methylcytosine shows that the cellular machinery of the fertilized egg cannot demethylate the second maternal genome in parthenogenetic, gynogenetic and triploid digynic embryos or remethylate the additional (already demethylated) paternal genome in androgenetic and triploid diandric embryos. This suggests that differential zygotic demethylation results from differences in the remodeling of paternal and maternal chromatin structures after fertilization, i.e. sperm nuclear decondensation and protamine-histone exchange. A proportion of embryos derived from normal matings display abnormal methylation patterns some of which are indistinguishable from those in androgenetic or gynogenetic embryos. We conclude that methylation reprogramming defects in mammalian zygotes contribute to the high incidence of early pregnancy failure.
Subject(s)
DNA Methylation , Embryo, Mammalian/physiology , Animals , Antibodies, Antinuclear , Antibodies, Monoclonal , Embryonic and Fetal Development , Female , Fluorescent Antibody Technique , Male , Mice , ParthenogenesisABSTRACT
Most cells contain the same set of genes and yet they are extremely diverse in appearance and functions. It is the selective expression and repression of genes that determines the specific properties of individual cells. Nevertheless, even when fully differentiated, any cell can potentially be reprogrammed back to totipotency, which in turn results in re-differentiation of the full repertoire of adult cells from a single original cell of any kind. Mechanisms that regulate this exceptional genomic plasticity and the state of totipotency are being unravelled, and will enhance our ability to manipulate stem cells for therapeutic purposes.
Subject(s)
Cell Differentiation/genetics , Genome , Stem Cells/physiology , Animals , Cell Nucleus , Genomic Imprinting , Germ Cells , Humans , Oocytes , ZygoteABSTRACT
Genomic imprinting confers a developmental asymmetry on the parental genomes, through epigenetic modifications in the germ line and embryo. These heritable modifications regulate the monoallelic activity of parental alleles resulting in their functional differences during development. Specific cis-acting regulatory elements associated with imprinted genes carry modifications involving chromatin structural changes and DNA methylation. Some of these modifications are initiated in the germ line. Comparative genomic analysis at imprinted domains is emerging as a powerful tool for the identification of conserved elements amenable to more detailed functional analysis, and for providing insight into the emergence of imprinting during the evolution of mammalian species. Genomic imprinting therefore provides a model system for the analysis of the epigenetic control of genome function.
Subject(s)
Gene Expression Regulation, Developmental , Genomic Imprinting , Germ Cells/metabolism , Alleles , DNA Methylation , Embryonic and Fetal Development , Evolution, Molecular , Female , Gametogenesis , Gene Silencing , Humans , Male , Oocytes/metabolism , RNA, Antisense/genetics , Regulatory Sequences, Nucleic Acid , Zygote/metabolismABSTRACT
Transient neonatal diabetes mellitus (TNDM) is associated with intra-uterine growth retardation, dehydration and a lack of insulin. Some TNDM patients exhibit paternal uniparental disomy (UPD) of chromosome 6q24, where at least two imprinted genes, HYMAI and ZAC, have so far been characterized. Here we show that the differentially methylated CpG island that partially overlaps mZac1 and mHymai at the syntenic mouse locus is a likely imprinting control region (ICR) for the approximately 120--200 kb domain. The region is unmethylated in sperm but probably methylated in oocytes, a difference that persists between parental alleles throughout pre- and post-implantation development. We also show that within this ICR, there is a region that exhibits a high degree of homology between mouse and human. Using a reporter expression assay, we demonstrate that this conserved region acts as a strong transcriptional repressor when methylated. Finally, we provide in vivo evidence that in the majority of TNDM patients with a normal karyotype, there is a loss of methylation within the highly homologous region. We propose that this ICR regulates expression of imprinted genes within the domain; epigenetic or genetic mutations of this region probably result in TNDM, possibly by affecting expression of ZAC in the pancreas and/or the pituitary.
Subject(s)
DNA Methylation , Diabetes Mellitus/genetics , Genomic Imprinting , Promoter Regions, Genetic , Alleles , Animals , Chromosomes, Human, Pair 6/genetics , Conserved Sequence , CpG Islands/genetics , Female , Gene Silencing , Genes, Reporter , HeLa Cells , Humans , In Vitro Techniques , Male , Mice , Mice, Inbred C57BL , Mice, Inbred Strains , Polymerase Chain Reaction , Sequence Analysis, DNA , Transcription, Genetic , TransfectionABSTRACT
Neuronatin (Nnat) is an imprinted gene that is expressed exclusively from the paternal allele while the maternal allele is silent and methylated. The Nnat locus exhibits some unique features compared with other imprinted domains. Unlike the majority of imprinted genes, which are organised in clusters and coordinately regulated, Nnat does not appear to be closely linked to other imprinted genes. Also unusually, Nnat is located within an 8-kb intron of the Bc10 gene, which generates a biallelically expressed, antisense transcript. A similar organisation is conserved at the human NNAT locus on chromosome 20. Nnat expression is first detected at E8.5 in rhombomeres 3 and 5, and subsequently, expression is widespread within postmitotic neuronal tissues. Using modified BAC transgenes, we show that imprinted expression of Nnat at ectopic sites requires, at most, an 80-kb region around the gene. Furthermore, reporter transgenes reveal distinct and dispersed cis-regulatory elements that direct tissue-specific expression and these are predominantly upstream of the region that confers allele-specific expression.
Subject(s)
Chromosomes, Artificial, Bacterial/genetics , Enhancer Elements, Genetic/genetics , Gene Expression Regulation , Genomic Imprinting/genetics , Membrane Proteins/genetics , Nerve Tissue Proteins/genetics , Transgenes/genetics , Alleles , Animals , Choristoma/genetics , Cloning, Molecular , Female , In Situ Hybridization , Introns/genetics , Male , Mice , Mice, Transgenic , Plasmids/genetics , Promoter Regions, Genetic/genetics , RNA, Messenger/analysis , RNA, Messenger/geneticsABSTRACT
Complex phenotypes and genotypes characterize the human disease, Beckwith--Wiedemann syndrome (BWS). Genetic and epigenetic mutations are found in five different genes which all lie within a 1 Mb imprinted domain on human chromosome 11p15. Only two of these genes, p57(KIP2) (CDKN1C) and IGF2, are likely to be functionally involved in this disease. The presence of the additional mutations therefore suggests a role for the regulation of these two genes by distant cis-elements. The mouse Igf2 gene is regulated by enhancers and imprinting elements which lie >120 kb downstream of its promoter. Here we show that key elements for expression of the mouse p57(Kip2) (Cdkn1c) gene also lie at a distance. Enhancers for expression within skeletal muscle and cartilage lie >25 kb downstream of the gene. In addition, we find no evidence for allele-specific expression of p57(Kip2) (Cdkn1c) from our bacterial artificial chromosome transgenes that span 315 kb around the locus. This suggests that a key imprinting element for p57(Kip2) (Cdkn1c) also lies at a distance. Therefore, BWS in humans may result from disruption of appropriate expression of the p57(KIP2) (CDKN1C) gene through mutations that occur at a substantial distance from the gene.
Subject(s)
Beckwith-Wiedemann Syndrome/genetics , Beckwith-Wiedemann Syndrome/metabolism , Genomic Imprinting , Nuclear Proteins/biosynthesis , Nuclear Proteins/genetics , Animals , Cartilage/metabolism , Chromosome Mapping , Contig Mapping , CpG Islands , Cyclin-Dependent Kinase Inhibitor p57 , Enhancer Elements, Genetic , Female , Genotype , Humans , In Situ Hybridization , Male , Methylation , Mice , Mice, Transgenic , Models, Genetic , Muscle, Skeletal/metabolism , Mutation , Phenotype , Promoter Regions, Genetic , Tissue Distribution , Transgenes , Translocation, GeneticABSTRACT
We have analysed Xist expression patterns in parthenogenetic and control fertilised preimplantation embryos by using RNA FISH. In normal XX embryos, maternally derived Xist alleles are repressed throughout preimplantation development. Paternal alleles are expressed as early as the 2-cell stage. In parthenogenetic embryos, we observed Xist RNA expression and accumulation from the morula stage onwards, indicating loss of maternal imprinting. In the majority of cells, expression was from a single allele, indicating that X chromosome counting occurs to establish appropriate monoallelic Xist expression. We discuss these data in the context of models for regulation of imprinted and random X inactivation.
Subject(s)
Blastocyst/metabolism , Genomic Imprinting , Alleles , Animals , DNA/metabolism , Fathers , Female , In Situ Hybridization, Fluorescence , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Models, Genetic , Mothers , Phenotype , RNA/metabolism , Time Factors , X Chromosome/geneticsABSTRACT
Mammalian parental genomes are not functionally equivalent, and both a maternal and paternal contribution is required for normal development. The differences between the parental genomes are the result of genomic imprinting--a form of gene regulation that results in monoallelic expression of imprinted genes. Cis-regulatory elements at imprinted loci are responsible for directing allele-specific epigenetic marks required for correct gene expression. This cis information must be interpreted at various points in development, including in the germline where existing imprints are erased and reset. Imprints must also be maintained during preimplantation development, when the genome undergoes dramatic global epigenetic changes.
Subject(s)
Antibody Diversity , Embryonic and Fetal Development/genetics , Animals , Biological Evolution , Female , Gene Expression Regulation, Developmental , Genome , Genomic Imprinting , Humans , Male , Models, Genetic , Polycomb-Group Proteins , Pregnancy , Repressor Proteins/geneticsABSTRACT
Polycomb-group (Pc-G) genes are required for the stable repression of the homeotic selector genes and other developmentally regulated genes, presumably through the modulation of chromatin domains. Among the Drosophila Pc-G genes, Enhancer of zeste [E(z)] merits special consideration since it represents one of the Pc-G genes most conserved through evolution. In addition, the E(Z) protein family contains the SET domain, which has recently been linked with histone methyltransferase (HMTase) activity. Although E(Z)-related proteins have not (yet) been directly associated with HMTase activity, mammalian Ezh2 is a member of a histone deacetylase complex. To investigate its in vivo function, we generated mice deficient for Ezh2. The Ezh2 null mutation results in lethality at early stages of mouse development. Ezh2 mutant mice either cease developing after implantation or initiate but fail to complete gastrulation. Moreover, Ezh2-deficient blastocysts display an impaired potential for outgrowth, preventing the establishment of Ezh2-null embryonic stem cells. Interestingly, Ezh2 is up-regulated upon fertilization and remains highly expressed at the preimplantation stages of mouse development. Together, these data suggest an essential role for Ezh2 during early mouse development and genetically link Ezh2 with eed and YY1, the only other early-acting Pc-G genes.
Subject(s)
Blastocyst/physiology , Drosophila Proteins , Embryonic and Fetal Development , Nuclear Proteins/genetics , Nuclear Proteins/physiology , Repressor Proteins/genetics , Repressor Proteins/physiology , Animals , Crosses, Genetic , Embryo Implantation , Female , Gastrula/physiology , Gene Targeting , Humans , In Situ Hybridization , Male , Mice , Mice, Transgenic , Multigene Family/genetics , Polycomb Repressive Complex 2 , Pregnancy , Reverse Transcriptase Polymerase Chain Reaction , Stem Cells/cytologyABSTRACT
Genomic imprinting is the phenomenon whereby mono-allelic expression of certain genes occurs depending on their parental origin. The observation that imprinting only occurs in placental mammals has led to the suggestion that it may play a role in this form of reproduction. In the present study we have investigated the pattern of expression of the human PEG3 gene in the early to term placenta, as well as the uterus and ovary, using RT-PCR, northern blot and in situ hybridization. A comparison is made with the expression of Peg3 in the mouse by histochemical staining in betageo knock out mice. We have demonstrated high levels of PEG3 in the human placenta and have localized the signal to the layer of villous cytotrophoblast cells. In contrast, the pattern of expression of Peg3 in the mouse placenta is less restricted, the message being present in all trophoblast populations. Thus, expression of PEG3/Peg3 in the human and mouse placenta is not directly comparable. We have also detected PEG3 message in the ovarian stroma. We have sequenced the human PEG3 gene from exon 3 to exon 9. By utilizing a polymorphism detected in exon 9, we have established that only the paternal allele is expressed in human placenta. Human PEG3 is therefore maternally imprinted as in mouse.