Microbial interactions facilitating efficient methane driven denitrification via in-situ utilization of short chain fatty acids.

High nitrate pollution in agriculture and industry poses a challenge to emerging methane oxidation coupled denitrification. In this study, an efficient nitrate removal efficiency of 100 % was achieved at an influent loading rate of 400 mg-N/L·d, accompanied by the production of short chain fatty acids (SCFAs) with a maximum value of 80.9 mg/L. Batch tests confirmed that methane was initially converted to acetate, which then served as a carbon source for denitrification. Microbial community characterization revealed the dominance of heterotrophic denitrifiers, including Simplicispira (22.8 %), Stappia (4.9 %), and the high­nitrogen-tolerant heterotrophic denitrifier Diaphorobacter (19.0 %), at the nitrate removal rate of 400 mg-N/L·d. Notably, the low abundance of methanotrophs ranging from 0.24 % to 3.75 % across all operational stages does not fully align with the abundance of pmoA genes, suggesting the presence of other functional microorganisms capable of methane oxidation and SCFAs production. These findings could facilitate highly efficient denitrification driven by methane and contributed to the development of denitrification using methane as an electron donor.

Sewage sludge derived biochar for environmental improvement: Advances, challenges, and solutions.

With the rapid growth yield of global sewage sludge, rational and effective treatment and disposal methods are becoming increasingly needed. Biochar preparation is an attractive option for sewage sludge treatment, the excellent physical and chemical properties of sludge derived biochar make it an attractive option for environmental improvement. Here, the current application state of sludge derived biochar was comprehensively reviewed, and the advances in the mechanism and capacity of sludge biochar in water contaminant removal, soil remediation, and carbon emission reduction were described, with particular attention to the key challenges involved, e.g., possible environmental risks and low efficiency. Several new strategies for overcoming sludge biochar application barriers to realize highly efficient environmental improvement were highlighted, including biochar modification, co-pyrolysis, feedstock selection and pretreatment. The insights offered in this review will facilitate further development of sewage sludge derived biochar, towards addressing the obstacles in its application in environmental improvement and global environmental crisis.