[DYNAMICS OF APOPTOTIC MARKERS (INTERLEUKIN-2 AND TNF-α) IN THE BLOOD SERUM OF CHILDREN WITH CHRONIC GASTRODUODENITES].

UNLABELLED: The aim was to investigate apoptotic dynamics by IL-2 and TNF-α levels in the blood serum of children with chronic gastroduodenites. MATERIALS AND METHODS: There were examined 90 children with chronic gastroduodenitis. Cytokine level in the serum was studied at admission and in dynamics. RESULTS: Chronic gastroduodenitis is accompanied by the increase of IL-2 and TNF-α concentration thus reflecting the activity of apoptotic processes. The decrease of the markers' (IL-2 and TNF-α) levels after 10-11 days, 3 and 9 months after treatment indicates the decrease of apoptotic activity. In hyperplastic and atrophic gastritis there has been registered the highest and the lowest concentration of the markers, respectively. H. pylori contributes to the TNF-α production mainly.

[A phototube connecting digital cameras with light optic microscopes].

We present here a phototube model making possible connection of a digital camera with light optic microscopes in order to obtain images of microobjects and for their further computer treatment. The advantage of this model is simplicity of its manufacturing and small required expenses as well as an increase in information density for microobject studies. This phototube has been covered by a patent for a useful model N2 48228 registered in the Public Register of RF on September 27, 2005.

[Comparison of methods of detection of bovine adenovirus serotype 3 in infected culture of calf kidney cells (MDBK)]. / Sopostavlenie metodov obnaruzheniia adenovirusa krupnogo rogatogo skota 3-go serotipa v zarazhennoi kul'ture perevivaemykh kletok pochki telenka (MDBK).

The comparative study of the dynamics of the main antigen (hexon) and viral DNA of the bovine adenovirus type 3 accumulation in the established cell line MDBK under the conditions of single- or multistep cycle of infection has been undertaken. The quantitative immunoelectrophoresis and immunoenzyme assay detected the viral antigens on the late stages of infection in the period of cellular monolayer degradation. The immunofluorescence reaction and histochemical immunoenzyme method detected the antigen in the infected cells concurrently with the primary expression of the viral cytopathic effect. The reaction of the spot molecular hybridization with the [32P]-DNA probes detected the viral DNA considerably earlier than the antigen was detected by the immunological methods, before the appearance of degenerative changes in the infected cells. Preference of the immunoenzyme assay and DNA-probes in diagnosis of the virus are discussed.