Subject(s)
Dog Diseases/transmission , Equipment and Supplies/microbiology , Pasteurella Infections/diagnostic imaging , Pets/microbiology , Respiratory Insufficiency/microbiology , Acute Disease , Animals , Dog Diseases/microbiology , Dogs/microbiology , Humans , Male , Middle Aged , Pasteurella Infections/drug therapy , Pasteurella Infections/transmission , Pasteurella multocida/pathogenicity , Phylogeny , Radiography , Thorax/diagnostic imaging , Thorax/microbiologyABSTRACT
BACKGROUND: The incidence of meningitis caused by Klebsiella pneumoniae (Kp) and Klebsiella oxytoca (Ko) in high-income countries is unknown, and no series have been published to date. METHODS: We conducted a nationwide multicenter observational study in France between 2006 and 2016. All children from the French national registry for paediatric bacterial meningitis under the age of 1 year and hospitalized for Kp or Ko meningitis were included. Virulence factors of four Klebsiella spp. strains were explored by whole genome sequencing. RESULTS: Of 1859 cases of meningitis in children under the age of 1 year, 13 cases (0.7%) of Klebsiella spp. meningitis (nine for Kp meningitis and four for Ko meningitis) were registered in the French national registry. Three of the patients died and 50% of the survivors had developmental delays. CONCLUSIONS: Prematurity, low birth weight, and congenital anomalies of the urinary tract appear to be risk factors for Klebsiella spp. meningitis as well as virulence factors of the strain.
Subject(s)
Klebsiella Infections/epidemiology , Klebsiella oxytoca/genetics , Klebsiella pneumoniae/genetics , Meningitis, Bacterial/epidemiology , Anti-Bacterial Agents/therapeutic use , Female , France/epidemiology , Humans , Incidence , Infant , Infant, Newborn , Klebsiella Infections/drug therapy , Magnetic Resonance Imaging , Male , Meningitis, Bacterial/drug therapy , Meningitis, Bacterial/microbiology , Registries , Risk Factors , Survival Rate , Virulence Factors/genetics , Whole Genome SequencingSubject(s)
Bacterial Proteins/analysis , Blood/microbiology , Gram-Negative Bacteria/enzymology , Microbial Sensitivity Tests/methods , Molecular Diagnostic Techniques/methods , Urine/microbiology , beta-Lactamases/analysis , Bacterial Proteins/genetics , Gram-Negative Bacteria/genetics , Gram-Negative Bacteria/isolation & purification , Humans , Sensitivity and Specificity , beta-Lactamases/geneticsABSTRACT
Melioidosis is an endemic disease in Southeast Asia and northern Australia. An increasing number of cases are being reported in nonendemic countries, making the diagnosis less obvious. We discuss the identification of Burkholderia pseudomallei using matrix-assisted desorption ionization-time of flight mass spectrometry on the occasion of recent cases of imported melioidosis in French travellers.
ABSTRACT
Effective antibiotic therapy is crucial for the outcome of septic patients and requires early diagnosis of ß-lactam resistance in cases of Gram-negative bacteremia. Here, we report high sensitivity of the ß-LACTA™ test in rapid detection of extended-spectrum ß-lactamase-producing Enterobacteriaceae in blood cultures positive for Gram-negative bacilli.