ABSTRACT
The rapid development of the Internet of Things (IoT) has extensively promoted the development of Wireless Sensor Networks (WSNs), an essential technology for series displaying perception and data collected from the physical world. In densely distributed areas, sensor nodes are unevenly distributed, which leads to the network coverage build-up and the consequent efficiency and effectiveness of WSNs. To address this issue, this paper proposes a new method for WSN coverage optimization based on the Reptile Search Algorithm (RSA). In the past, the Reptile Search algorithm has been used to solve optimization problems, which means it can improve different processes. However, the RSA needs to track the trajectory of optimal individuals in each iteration, which will ignore non-optimal individuals' bioeconomic characteristics. Therefore, the paper introduces a distribution estimation strategy into the RSA framework, which can fully mine all the positional information hidden in the entire population. We selected several functions as optimization test benchmark functions to evaluate the feasibility of the proposed method. This paper compares the proposed improved RSA with the standard RSA and some traditional optimization algorithms. The result has been calculated through a series of experiments on network coverage optimization, and the change of parameters also determines the effect of the RSA in the optimization of network coverage. The simulated results of the three similar network coverage optimization experiments show that the improved RSA can be used efficiently within different scenarios.
ABSTRACT
Although hydrazine (N2H4) is a versatile chemical used in many applications, it is toxic, and its leakage may pose a threat to both human health and environments. Consequently, the monitoring of N2H4 is significant. This study reports a one-step synthesis for coumarin-based ratiometric fluorescent probe (FP) CHAC, with acetyl as the recognition group. Selected deprotection of the acetyl group via N2H4 released the coumarin fluorophore, which recovered the intramolecular charge transfer process, which caused a prominent fluorescent, ratiometric response. CHAC demonstrated the advantages of high selectivity, a strong capacity for anti-interference, a low limit of detection (LOD) (0.16 µM), a large linear detection range (0-500 µM), and a wide effective pH interval (6-12) in N2H4 detection. Furthermore, the probe enabled quantitative N2H4 verifications in environmental water specimens in addition to qualitative detection of N2H4 in various soils and of gaseous N2H4. Finally, the probe ratiometrically monitored N2H4 in living cells having low cytotoxicity.
Subject(s)
Coumarins , Fluorescent Dyes , Humans , Fluorescent Dyes/chemistry , HeLa Cells , Coumarins/chemistry , Limit of Detection , Hydrazines/chemistry , Spectrometry, FluorescenceABSTRACT
Psoriasis, an inflammatory autoimmune skin disease, is characterized by scaly white or erythematous plaques, which severely influence patients' quality of life and social activities. Mesenchymal stem cells derived from the human umbilical cord (UCMSCs) represent a promising therapeutic approach for psoriasis because of its unique superiority in ethical agreeableness, abundant source, high proliferation capacity, and immunosuppression. Although cryopreservation provided multiple benefits to the cell therapy, it also greatly compromised clinical benefits of MSCs due to impaired cell functions. The current study aims to evaluate the therapeutic efficacy of cryopreserved UCMSCs in a mouse model of psoriasis as well as in patients with psoriasis. Our results showed that cryopreserved and fresh UCMSCs have comparable effects on the suppression of psoriasis-like symptoms such as thickening, erythema, and scaling, and serum IL-17 A secretion in mice model of psoriasis. Moreover, psoriatic patients injected with cryopreserved UCMSCs had a significant improvement in the Psoriasis Area and Severity Index (PASI), Physician Global Assessment (PGA), and Patient Global Assessments (PtGAs) scores compared to baseline values. Mechanically, cryopreserved UCMSCs markedly inhibit the proliferation of PHA-activated PBMCs, type 1 T helper (Th1) and type 17 T helper (Th17) cell differentiation and secretion of inflammatory cytokines including IFN-γ, TNF-a and IL-17 A in PBMCs stimulated by anti-CD3/CD28 beads. Taken together, these data indicated that cryopreserved UCMSCs exhibited great beneficial effect on psoriasis. Thus, cryopreserved UCMSCs can be systemically administered as ''off-the-shelf'' cell product for psoriasis therapy. Trial Registration ChiCTR1800019509. Registered on November 15, 2018-Retrospectively registered, http://www.chictr.org.cn/ .
Subject(s)
Mesenchymal Stem Cells , Psoriasis , Mice , Animals , Humans , Interleukin-17/metabolism , Quality of Life , Psoriasis/therapy , Psoriasis/metabolism , Umbilical CordABSTRACT
Mastering nuclear fusion, which is an abundant, safe, and environmentally competitive energy, is a great challenge for humanity. Tokamak represents one of the most promising paths toward controlled fusion. Obtaining a high-performance, steady-state, and long-pulse plasma regime remains a critical issue. Recently, a big breakthrough in steady-state operation was made on the Experimental Advanced Superconducting Tokamak (EAST). A steady-state plasma with a world-record pulse length of 1056 s was obtained, where the density and the divertor peak heat flux were well controlled, with no core impurity accumulation, and a new high-confinement and self-organizing regime (Super I-mode = I-mode + e-ITB) was discovered and demonstrated. These achievements contribute to the integration of fusion plasma technology and physics, which is essential to operate next-step devices.
ABSTRACT
Hybrid organic-inorganic lead halide perovskites (HOIPs) possess significant photoelectric characteristics for solar energy conversion, but the presence of lead causes issues for eco-friendly applications. Halide double perovskites represent a green option for application in the optoelectronic field, especially X-ray detection systems. Despite the great efforts, the exploration of large-size lead-free iodide-based hybrid double perovskite single crystals for X-ray detection has been unsuccessful. Herein, we demonstrate that a large single crystal of the 2D (two-dimensional) semiconducting perovskite (C6H16N2)2CuBiI8·0.5H2O can serve as an X-ray detection candidate. A perovskite crystal, as large as 35 × 31 × 3 mm3, was grown using a low-cost, simple cooling solution approach. To the best of our knowledge, this is the first time a centimeter-sized 2D BiCu iodide double perovskite single crystal has been used for X-ray detection. The perovskite crystal exhibited unique properties for X-ray detection, such as a significant X-ray absorption coefficient, considerable µτ product, and low trap density. Moreover, X-ray detection with a sensitivity of 5.51 µC Gyair-1 cm-2 was achieved based on a single crystal. This work opens new ways to explore specially designed organic cations for stabilizing 2D HOIPs that show great potential in optoelectronics.
ABSTRACT
Influenza hemagglutinin that drives viral entry into cells via the membrane fusion process is an up-and-coming antiviral drug target. Herein, we described for the first time the design, synthesis, and biological characteristics of a new class of pentacyclic triterpenoid-based proteolysis targeting chimeras (PROTACs) to enhance the degradation of hemagglutinin target. Among these PROTACs, V3 showed the best degradation effect on the hemagglutinin with a median degradation concentration of 1.44 µM in a ubiquitin and proteasome-dependent manner and broad-spectrum anti-influenza A virus activity but not affected the entry of influenza virus. Moreover, intravenous injection of V3 protected mice against influenza A virus-induced toxic effects. Further diazirine-containing photo-crosslinking mass spectrometric analysis of hemagglutinin complexes indicated crosslinking to Asn15, Thr31, and Asn27, a novel target of hemagglutinin. Taken together, our data revealed that oleanolic acid-based PROTACs could degrade hemagglutinin protein, providing a new direction toward the discovery of potential anti-influenza drugs.
Subject(s)
Influenza, Human , Triterpenes , Animals , Chimera/metabolism , Hemagglutinins , Humans , Influenza, Human/drug therapy , Intercellular Signaling Peptides and Proteins , Mice , Proteins/metabolism , Proteolysis , Triterpenes/chemistryABSTRACT
A novel fluorescence-enhanced probe 2-butyl-1,3-dioxoisoindolin-4-yl picolinate (BDIP) has been developed for the detection of Cu2+ based on the excited-state intramolecular proton transfer (ESIPT) process. BDIP utilized a phthalimide derivative as the fluorophore and selected picolinate ester as the recognition site for Cu2+. The probe displayed high selectivity, strong anti-interference ability, and a significant fluorescence enhancement effect for Cu2+ in phosphate buffer saline (PBS, 10 mM, pH 7.4) with the detection limit of 31 nM. BDIP also possesses the advantages of simple synthesis steps, large Stokes shift, and good water solubility. Moreover, BDIP was used for Cu2+ detection in real water samples, with the result being satisfactory.
Subject(s)
Fluorescent Dyes , Protons , Fluorescent Dyes/chemistry , Phthalimides/chemistry , Spectrometry, Fluorescence , WaterABSTRACT
Both MLL-AF9 and MLL-ENL leukemia fusion proteins drive oncogenic transformation of hematopoietic cells through their N-terminal DNA/histone binding mixed-lineage leukemia 1 domain and C-terminal fragment of AF9 or ENL containing an unstructured linker region and the ANC1 homology domain, which recruits transcription factors. Despite of their structural similarity, acute myeloid leukemia (AML) patients bearing MLL-ENL show more adverse outcomes compared to those with MLL-AF9. We recapitulated the clinical patterns of these two MLL-fusions driven AMLs using murine models and found that MLL-ENL AML cells showed slower cell cycle progression and more resistance to standard chemotherapy than MLL-AF9 cells. These phenotypes were primarily controlled by the linker regions of ENL and a highly conserved lysine residue K469 within. Substitution of K469 with an acetylated mimic glutamine abolished the ability of MLL-ENL to suppress proliferation and promote chemo-resistance. We showed that deacetylase Sirt2 might act as an upstream regulator of MLL-ENL. Deletion of Sirt2 promoted proliferation of AML cells with either MLL fusions. Importantly, loss of Sirt2 greatly enhanced the sensitivity of the MLL-ENL AML cells to chemo-treatment. Taken together, our study uncovered a unique regulatory role of Sirt2 in leukemogenesis and suggested targeting SIRT2 as a new way to sensitize MLL-ENL AML patience for chemotherapy.
Subject(s)
Cell Proliferation/genetics , Gene Expression Regulation, Leukemic/genetics , Leukemia, Myeloid/genetics , Myeloid-Lymphoid Leukemia Protein/genetics , Oncogene Proteins, Fusion/genetics , Sirtuin 2/genetics , Acute Disease , Amino Acid Sequence , Animals , Antineoplastic Combined Chemotherapy Protocols/pharmacology , Carcinogenesis/genetics , Carcinogenesis/metabolism , Carcinogenesis/pathology , Cytarabine/administration & dosage , Doxorubicin/administration & dosage , Gene Expression Regulation, Leukemic/drug effects , Kaplan-Meier Estimate , Leukemia, Myeloid/drug therapy , Leukemia, Myeloid/metabolism , Mice, Inbred C57BL , Mice, Knockout , Myeloid-Lymphoid Leukemia Protein/metabolism , Oncogene Proteins, Fusion/metabolism , Sequence Homology, Amino Acid , Sirtuin 2/metabolism , Tumor Cells, CulturedABSTRACT
It is urgently necessary to develop more effective anti-influenza agents due to the continuous emergence of drug-resistant strains of influenza virus. Our earlier studies have identified that certain pentacyclic triterpene derivatives are effective inhibitors of influenza virus infection. In the present study, a series of C-28 modified pentacyclic triterpene derivatives via conjugation with a series of polyphenols were synthesized, and their antiviral activities against influenza A/WSN/33 (H1N1) virus in MDCK (Madin-Darby canine kidney) cells were evaluated. Four compounds 23m, 23o, 23q and 23s displayed robust anti-influenza potency with averaged IC50 values at the low-micromole level, surpassing the potency of oseltamivir. In addition, the in vitro cytotoxic activity of the four conjugates against MDCK cells showed no toxicity at 100⯵M. Further mechanism studies of compound 23s, one of the best representative conjugates with IC50 value of 5.80⯵M and a selective index (SI) value of over 17.2, by hemagglutination inhibition (HI), surface plasmon resonance and molecular modeling indicated that this conjugate bound tightly to the viral envelope hemagglutinin (KDâ¯=â¯15.6⯵M), thus blocking the invasion of influenza viruses into host cells.
Subject(s)
Antiviral Agents/chemical synthesis , Pentacyclic Triterpenes/pharmacology , Polyphenols/chemistry , Animals , Antiviral Agents/pharmacology , Dogs , Hemagglutination/drug effects , Humans , Influenza A Virus, H1N1 Subtype , Inhibitory Concentration 50 , Madin Darby Canine Kidney Cells , Models, Molecular , Orthomyxoviridae Infections/drug therapy , Pentacyclic Triterpenes/chemical synthesis , Structure-Activity Relationship , Virus Internalization/drug effectsABSTRACT
The emergence of drug resistant variants of the influenza virus has led to a great need to identify novel and effective antiviral agents. In our previous study, a series of sialic acid (C-2 and C-4)-pentacyclic triterpene conjugates have been synthesized, and a five-fold more potent antiviral activity was observed when sialic acid was conjugated with pentacyclic triterpene via C-4 than C-2. It was here that we further reported the synthesis and anti-influenza activity of novel sialic acid (C-5 and C-9)-pentacyclic triterpene conjugates. Their structures were confirmed by ESI-HRMS, ¹H-NMR, and 13C-NMR spectroscopic analyses. Two conjugates (26 and 42) showed strong cytotoxicity to MDCK cells in the CellTiter-Glo assay at a concentration of 100 µM. However, they showed no significant cytotoxicity to HL-60, Hela, and A549 cell lines in MTT assay under the concentration of 10 µM (except compound 42 showed weak cytotoxicity to HL-60 cell line (10 µM, ~53%)). Compounds 20, 28, 36, and 44 displayed weak potency to influenza A/WSN/33 (H1N1) virus (100 µM, ~20-30%), and no significant anti-influenza activity was found for the other conjugates. The data suggested that both the C-5 acetylamide and C-9 hydroxy of sialic acid were important for its binding with hemagglutinin during viral entry into host cells, while C-4 and C-2 hydroxy were not critical for the binding process and could be replaced with hydrophobic moieties. The research presented herein had significant implications for the design of novel antiviral inhibitors based on a sialic acid scaffold.
Subject(s)
Antiviral Agents/chemical synthesis , Antiviral Agents/pharmacology , Influenza A Virus, H1N1 Subtype/drug effects , N-Acetylneuraminic Acid/chemistry , Triterpenes/chemical synthesis , Triterpenes/pharmacology , Animals , Antiviral Agents/chemistry , Carbon-13 Magnetic Resonance Spectroscopy , Cell Line, Tumor , Dogs , Hemagglutinin Glycoproteins, Influenza Virus/chemistry , Humans , Madin Darby Canine Kidney Cells , Proton Magnetic Resonance Spectroscopy , Spectrometry, Mass, Electrospray Ionization , Triterpenes/chemistryABSTRACT
Sialic acid derivatives, analogs, and their conjugates are important pharmacophores. Modification of the C-4 hydroxyl group of sialic acid can lead to derivatives, such as zanamivir, with potent anti-influenza activities. Herein, we described the synthesis of C-4-modified sialic acid derivatives via conjugation with naturally derived pentacyclic triterpenes, which are active ingredients of traditional Chinese medicine, and the evaluation of their in vitro anti-influenza virus activity in MDCK cells. Interestingly, a set of configurational isomers was obtained during the de-O-acetylation reaction of two pentacyclic triterpene-sialic acid conjugates under Zemplén conditions, and a mechanism was proposed. Owing to the attachment of the Neu5Ac2en moiety, all synthesized conjugates displayed lower hydrophobicity than their parent compounds. In comparison with ursane- and lupane-type triterpenes, oleanane-type triterpene-functionalized Neu5Ac2en conjugates were most promising. The insertion of a (1,2,3-triazol-4-yl)-methyl between the amide bond and Neu5Ac2en caused a substantial decrease in activity. Compound 15a exhibited the highest inhibitory activity (IC50 = 8.3 µM) and selectivity index (SI = 22.7). Further studies involving hemagglutination inhibition and neuraminidase inhibition suggested that compound 15a inhibited virus-induced hemagglutination with no effect on the enzymatic activity of neuraminidase, indicating that the antiviral activity appeared to be mediated via interaction with hemagglutinin at the initial stage of viral infection.
ABSTRACT
N-tert-Butyl-N,N'-diacylhydrazines are nonsteroidal ecdysone agonists used as environmental benign pest regulators. In this paper, two series of new N-tert-butyl-N,N'-diacylhydrazine derivatives containing 1,2,3-thiadiazole were designed and synthesized. All structures of the synthesized compounds were confirmed by proton nuclear magnetic resonance ((1)H NMR), infrared spectroscopy (IR), and high-resolution mass spectrometry (HRMS). Bioasssay results indicated that most of the synthesized compounds possessed good insecticidal activities against Plutella xylostella L. and Culex pipiens pallens as compared with the positive control, tebufenozide. The results of this study indicated that 1,2,3-thiadiazoles, as an important active substructure, could improve or maintain the activity of the dicylhydrazines and favor novel pesticide development.