ABSTRACT
We evaluated the BYSL content and underlying mechanism in melanoma (SKCM) overall survival (OS). In this study, we used a comprehensive approach combining bioinformatics tools, including miRNA estimation, quantitative real-time polymerase chain reaction (qRT-PCR) of miRNAs, E3 ligase estimation, STRING analysis, TIMER analysis, examination of associated upstream modulators, protein-protein interaction (PPI) analysis, as well as retrospective and survival analyses, alongside clinical sample validation. These methods were used to investigate the content of BYSL, its methylation status, its relation to patient outcome, and its immunologic significance in tumors. Our findings revealed that BYSL expression is negatively regulated by BYSL methylation. Analysis of 468 cases of SKCM RNA sequencing samples demonstrated that enhanced BYSL expression was associated with higher tumor grade. We identified several miRNAs, namely hsa-miR-146b-3p, hsa-miR-342-3p, hsa-miR-511-5p, hsa-miR-3690, and hsa-miR-193a-5p, which showed a strong association with BYSL levels. Furthermore, we predicted the E3 ubiquitin ligase of BYSL and identified CBL, FBXW7, FZR1, KLHL3, and MARCH1 as potential modulators of BYSL. Through our investigation, we discovered that PNO1, RIOK2, TSR1, WDR3, and NOB1 proteins were strongly associated with BYSL expression. In addition, we found a close association between BYSL levels and certain immune cells, particularly dendritic cells (DCs). Notably, we observed a significant negative correlation between miR-146b-3p and BYSL mRNA expression in SKCM sera samples. Collectively, based on the previously shown evidences, BYSL can serve as a robust bioindicator of SKCM patient prognosis, and it potentially contributes to immune cell invasion in SKCM.
Subject(s)
Biomarkers, Tumor , Gene Expression Regulation, Neoplastic , Melanoma , MicroRNAs , Humans , Melanoma/mortality , Melanoma/genetics , Melanoma/diagnosis , Melanoma/metabolism , Prognosis , MicroRNAs/genetics , Female , Male , Computational Biology/methods , DNA Methylation , RNA, Long Noncoding/genetics , Ubiquitin-Protein Ligases/genetics , Ubiquitin-Protein Ligases/metabolism , Middle Aged , Protein Interaction Maps , Gene Expression Profiling , Survival AnalysisABSTRACT
Poly(AM-co-DVB) was synthesized by acrylamide(AM) and divinylbenzene(DVB) via the crosslinking reaction. The microscope structure and thermal stability of Poly(AM-co-DVB) were characterized by FT-IR, SEM and TG. Congo red (CR) was used to measure the adsorptive capacity of Poly (AM-co-DVB). The effects of initial pH, contact time and temperature on the adsorption of CR on Poly (AM-co-DVB) were investigated in this work. The kinetics, equilibrium, and thermodynamics of the adsorption process were also discussed. The results showed that the maximum adsorption capacities were 319.1 mg x g(-1) at pH = 7.25 and contact time = 3 h. The adsorption kinetics was well fitted by a pseudo-second-order model and the adsorption isotherms agreed well with the Langmuir model. The adsorption process was spontaneous process. Above all, the adsorption capacity of Poly (AM-co-DVB) on Congo red is significant.