Effects of pairing on color change and central gene expression in lined seahorses.

Social monogamy is a reproductive strategy characterized by pair living and defense of a common territory. Pair bonding, sometimes displayed by monogamous species, is an affective construct that includes preference for a specific partner, distress upon separation, and the ability of the partner to buffer against stress. Many seahorse species show a monogamous social structure in the wild, but their pair bond has not been well studied. We examined the gene expression of lined seahorses (Hippocampus erectus) during and after the process of pairing in the laboratory as well as color change (luminance), a potential form of social communication and behavioral synchrony between pair mates. When a seahorse of either sex was interacting with its pair mate, their changes in luminance ("brightness") were correlated and larger than when interacting with an opposite-sex stranger. At the conclusion of testing, subjects were euthanized, RNA was extracted from whole brains and analyzed via RNA sequencing. Changes in gene expression in paired males versus those that were unpaired included processes governing metabolic activity, hormones and cilia. Perhaps most interesting is the overlap in gene expression change induced by pairing in both male seahorses and male prairie voles, including components of hormone systems regulating reproduction. Because of our limited sample size, we consider our results and interpretations to be preliminary, and prompts for further exploration. Future studies will expand upon these findings and investigate the neuroendocrine and genetic basis of these behaviors.

Evaluation of a Zinc Gluconate Neutralized with Arginine Product as a Nonsurgical Method for Sterilization of Rhesus Macaques (Macaca mulatta).

Because rhesus macaques (Macaca mulatta) are prolific breeders, overpopulation can be problematic in both research and feral populations. Currently, the most effective contraceptive methods are hormonal control in female macaques and vasectomies in males. These methods each come with innate challenges, the foremost being the alteration of necessary hormonal patterns. In this study, we assessed the use of zinc gluconate neutralized with arginine as a novel, nonsurgical alternative to male contraception in 12 rhesus macaques. This FDA-approved product for dogs is given as a one-time, intratesticular injection to cause permanent infertility yet theoretically spare the testosterone-producing Leydig cells of the testis. CBC counts, serum biochemistry analyses, testosterone levels, and testicular widths were evaluated at the time of injection and at 1 wk, 1 mo, 2 mo, or 3 mo afterward. Daily postinjection observations revealed transient scrotal enlargement in 8 of the 12 macaques but no indications of pain. In addition, full necropsies including testicular histopathology were assessed at study endpoints. Although some portion of every testis had evidence of seminiferous tubule loss, normal spermatogenesis was present in 22 of the 24 testes. In conclusion, chemical castration with the tested zinc gluconate neutralized with arginine product is not an effective method for sterilization of male rhesus macaques.