ABSTRACT
OBJECTIVES: It is very important that the effects of surface modified titanium on osteogenic differentiation of bone marrow mesenchymal stem cells in the process of bone regeneration. The bio-function of modified titanium could be affected by the inflammatory micro-environment. The aim of this study was to investigate the effects of modified titanium on osteogenic differentiation in the inflammatory conditions and the osteogenic properties of the modified titanium dental implant in vivo. METHODS: The medical pure titanium metals (PT-Ti) subjected to Anodic Oxidation (AO-Ti), Sand Blasting/acid etching (SLA-Ti) and Plasma-sprayed HA coating (HA coating-Ti) were used for regulating the osteogenic properties of MSCs in the normal and inflammatory conditions. RESULTS: The amount of the MSCs in the inflammatory environment were more similar to that in the non-inflammatory environment after cultured on AO-Ti samples for 7D. However, the proliferation of the MSCs was obviously inhibited on the other groups in the inflammatory condition. The morphology of MSC cells on the modified titanium surface was affected in the inflammatory conditions and the AO-Ti was more conducive to maintain the skeletal morphology of MSCs. The results of osteogenic related proteins expression showed that the amount of BMP-2 on AO-Ti group was the highest in the inflammatory conditions, and followed the order of AO-Ti > HA coating-Ti > SLA-Ti > PT-Ti. What's more, the AO-Ti samples were more beneficial to promote the expression of osteogenic genes ALP, OCN, COL-I and Runx2 in the inflammatory conditions. The results of osteogenic properties in vivo showed that the gingival depth of the AO-Ti group was smaller than that on the other groups. Some new bone could be observed around the AO-Ti implant at two weeks. The bone binding rates on AO-Ti group was the highest of 81.3% after implanted for one year. SIGNIFICANCE: The AO-Ti was beneficial to osteogenic differentiation than other modified titanium metals in inflammatory condition. The anodic oxidation is an effective surface modification method on titanium to promote bone regeneration.
Subject(s)
Osteogenesis , Titanium , Bone Regeneration , Cell Differentiation , GingivaABSTRACT
Objective:To retrieve, evaluate, and integrate the evidence on perioperative nutritional management in patients with bladder cancer, and summarize the optimal evidence to inform clinical practice.Methods:Evidence on nutritional management, including guidelines, systematic reviews, evidence summaries, best practices, and expert consensus, was retrieved from BMJ Best Practice, UpToDate, Registered Nurses' Association of Ontario Evidence-Based Nursing Care Guidelines, Scottish Intercollegiate Guidelines Network, National Institute for Health and Care Excellence, European Society for Clinical Nutrition and Metabolism, Cochrane Library, PubMed, CINAHL, Springer Link, Elsevier Science Direct, OVID, Medlinker, China National Knowledge Infrastructure, Wanfang Data, VIP Database for Chinese Technical Periodicals, and China National Biomedical Literature Database. Records published from the establishment of the databases until December 31, 2022 were included. The methodological quality of the guidelines was assessed using the Appraisal of Guidelines for Research and Evaluation II tool, and other types of studies were evaluated using the corresponding criteria in the Joanna Briggs Institute Database of Systematic Reviews and Implementation Reports (2016 edition).Result:A total of 11 articles were included, and four themes were identified based on expert panel review, namely nutritional screening and assessment, nutritional support, intestinal management, and health education, on which a total of 24 pieces of best evidence were summarized.Conclusions:This study used an evidence-based approach to generate the best evidence on perioperative nutritional management in patients with bladder cancer, providing solid rationales for standardized nutritional care in these patients. In clinical practice, healthcare professionals should take in to account the clinical environment, patient conditions, and patient preferences, and translat the evidence to tailored management plan to improve patients' clinical outcomes and quality of life.
ABSTRACT
Objective To assess the effectiveness of Chinese medicine treatment in endometriosis(EMT) by evidence-based medicine methods.Methods Retrieve articles mainly from Wanfang data,VIP information,CNKI and China TCM Database.Bring the articles report into research on the basis of strict quality evaluation.Consolidate cure rate and effective rate for meta-analysis.Results Based on 8 articles report,cure rate of Chinese medicine treatment is superior to western medicine treatment,and effective rate is equivalent to western medicine treatment.There is no side effect and other harmful consequence in evidence.Conclusions Chinese medicine treatment is superior to western medicine treatment in treating EMT.However,existence of some data methodological problems maybe directly affect the authenticity of the findings.So,more rigorous,randomized controlled trials should be designed in order to draw more reliable conclusions.