Genome-wide identification and expression analysis of TPP gene family under salt stress in peanut (Arachis hypogaea L.).

Trehalose-6-phosphate phosphatase (TPP), a key enzyme for trehalose biosynthesis in plants, plays a pivotal role in the growth and development of higher plants, as well as their adaptations to various abiotic stresses. Employing bioinformatics techniques, 45 TPP genes distributed across 17 chromosomes were identified with conserved Trehalose-PPase domains in the peanut genome, aiming to screen those involved in salt tolerance. Collinearity analysis showed that 22 TPP genes from peanut formed homologous gene pairs with 9 TPP genes from Arabidopsis and 31 TPP genes from soybean, respectively. Analysis of cis-acting elements in the promoters revealed the presence of multiple hormone- and abiotic stress-responsive elements in the promoter regions of AhTPPs. Expression pattern analysis showed that members of the TPP gene family in peanut responded significantly to various abiotic stresses, including low temperature, drought, and nitrogen deficiency, and exhibited certain tissue specificity. Salt stress significantly upregulated AhTPPs, with a higher number of responsive genes observed at the seedling stage compared to the podding stage. The intuitive physiological effect was reflected in the significantly higher accumulation of trehalose content in the leaves of plants under salt stress compared to the control. These findings indicate that the TPP gene family plays a crucial role in peanut's response to abiotic stresses, laying the foundation for further functional studies and utilization of these genes.

Genome-wide identification, evolution, and expression of the SNARE gene family in wheat resistance to powdery mildew.

SNARE proteins mediate eukaryotic cell membrane/transport vesicle fusion and act in plant resistance to fungi. Herein, 173 SNARE proteins were identified in wheat and divided into 5 subfamilies and 21 classes. The number of the SYP1 class type was largest in TaSNAREs. Phylogenetic tree analysis revealed that most of the SNAREs were distributed in 21 classes. Analysis of the genetic structure revealed large differences among the 21 classes, and the structures in the same group were similar, except across individual genes. Excluding the first homoeologous group, the number in the other homoeologous groups was similar. The 2,000 bp promoter region of the TaSNARE genes were analyzed, and many W-box, MYB and disease-related cis-acting elements were identified. The qRT-PCR-based analysis of the SNARE genes revealed similar expression patterns of the same subfamily in one wheat variety. The expression patterns of the same gene in resistant/sensitive varieties largely differed at 6 h after infection, suggesting that SNARE proteins play an important role in early pathogen infection. Here, the identification and expression analysis of SNARE proteins provide a theoretical basis for studies of SNARE protein function and wheat resistance to powdery mildew.