ABSTRACT
NonSMC condensin I complex subunit D2 (NCAPD2) is a newly identified oncogene; however, the specific biological function and molecular mechanism of NCAPD2 in liver cancer progression remain unknown. In the present study, the aberrant expression of NCAPD2 in liver cancer was investigated using public tumor databases, including TNMplot, The Cancer Genome Atlas and the International Cancer Genome Consortium based on bioinformatics analyses, and it was validated using a clinical cohort. It was revealed that NCAPD2 was significantly upregulated in liver cancer tissues compared with in control liver tissues, and NCAPD2 served as an independent prognostic factor and predicted poor prognosis in liver cancer. In addition, the expression of NCAPD2 was positively correlated with the percentage of Ki67+ cells. Finally, singlecell sequencing data, geneset enrichment analyses and in vitro investigations, including cell proliferation assay, Transwell assay, wound healing assay, cell cycle experiments, cell apoptosis assay and western blotting, were carried out in human liver cancer cell lines to assess the biological mechanisms of NCAPD2 in patients with liver cancer. The results revealed that the upregulation of NCAPD2 enhanced tumor cell proliferation, invasion and cell cycle progression at the G2/Mphase transition, and inhibited apoptosis in liver cancer cells. Furthermore, NCAPD2 overexpression was closely associated with the phosphatidylinositol 3kinase (PI3K)Aktmammalian target of rapamycin (mTOR)/cMyc signaling pathway and epithelialmesenchymal transition (EMT) progression in HepG2 and Huh7 cells. In addition, upregulated NCAPD2 was shown to have adverse effects on overall survival and diseasespecific survival in liver cancer. In conclusion, the overexpression of NCAPD2 was shown to lead to cell cycle progression at the G2/Mphase transition, activation of the PI3KAktmTOR/cMyc signaling pathway and EMT progression in human liver cancer cells.
Subject(s)
Cell Proliferation , Liver Neoplasms , Phosphatidylinositol 3-Kinases , Proto-Oncogene Proteins c-akt , Signal Transduction , TOR Serine-Threonine Kinases , Humans , TOR Serine-Threonine Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Liver Neoplasms/genetics , Liver Neoplasms/pathology , Liver Neoplasms/metabolism , Signal Transduction/genetics , Phosphatidylinositol 3-Kinases/metabolism , Male , Female , Cell Proliferation/genetics , Carcinogenesis/genetics , Carcinogenesis/pathology , Carcinogenesis/metabolism , Middle Aged , Gene Expression Regulation, Neoplastic , Disease Progression , Cell Line, Tumor , Cell Cycle Proteins/metabolism , Cell Cycle Proteins/genetics , Epithelial-Mesenchymal Transition/genetics , Apoptosis/genetics , Cell Movement/genetics , PrognosisABSTRACT
The four miniature heat pipes filled with DI water and SiO2-water nanofluids containing different volume concentrations (0.2%, 0.6% and 1.0%) are experimentally measured on the condition of air and water cooling. The wall temperature and the thermal resistance are investigated for three inclination angles. At the same of inlet heat water temperature in the heat system, it is observed that the total wall temperatures on the evaporator section are almost retaining constant by air cooling and the wall temperatures at the front end of the evaporator section are slightly reduced by water cooling. However, the wall temperatures at the condenser section using SiO2-water nanofluids are all higher than that for DI water on the two cooling conditions. As compared with the heat pipe using DI water, the decreasing of the thermal resistance in heat pipe using nanofluids is about 43.10%-74.46% by air cooling and 51.43%-72.22% by water cooling. These indicate that the utilization of SiO2-water nanofluids as working fluids enhances the performance of the miniature heat pipe. When the four miniature heat pipes are cut to examine at the end of the experiment, a thin coating on the surface of the screen mesh of the heat pipe using SiO2-water nanofluids is found. This may be one reason for reinforcing the heat transfer performance of the miniature heat pipe.