Construction of Aptamer-Functionalized DNA Hydrogels for Effective Inhibition of Shiga Toxin II Toxicity.

Bacterial infections have been seriously endangering public health and life, making it imperative to explore novel anti-infection strategies for their control. Herein, we constructed a DNA hydrogel encoded with aptamers (Apt-hydrogel) to inhibit Shiga toxin II (Stx2) toxicity, thereby alleviating Escherichia coli (EHEC) infection. The Apt-hydrogel was formed by two Y-shaped DNA scaffolds through rational design, where one end of Y was encoded with an aptamer sequence targeting the B subunit of Shiga toxin II (Stx2B). The Apt-hydrogel not only retained the high affinity of the aptamer but also provided protection for the aptamer, endowing it with better stability and biocompatibility. The results from in vitro and in vivo demonstrated good mediation effects of the Apt-hydrogel on Stx2 toxicity and confirmed its excellent inhibition activity. We hypothesized that the mechanism could be attributed to the high affinity of Apt-hydrogel for Stx2B, which effectively occupies the active site of Stx2B and its receptor Gb3. This interaction enhanced steric hindrance, thereby mediating their interaction and preventing Stx2 from entering the cell to exert toxicity. We anticipate that the novel Apt-hydrogel will expand the usage of aptamers and provide a new dimension for the Apt-hydrogel as a promising blocking assistant to inhibit Shiga toxin infections via a strong steric hindrance effect.

[Stabilization of Cd-contaminated Soil with Thiol-modified Biochar and Response of Soil Microorganisms].

To explore the stabilization effect of livestock manure biochar on Cd-contaminated soil and its impact on the soil environment, a pot experiment was conducted to investigate the stabilization efficiency of cattle manure-biochar （BC） and thiol-modified biochar （SBC） on Cd in soil and their effect on the soil properties and microbial community. The structural equation model （SEM） was used to analyze the effect pathways of BC and SBC on the soil microbial community. The results showed that BC and SBC increased soil pH, available potassium, available phosphorus, and organic matter content but decreased soil available nitrogen content compared with those in CK. The stabilization efficiency of BC for Cd in soil was 14.97%, which was much lower than that of SBC （85.71%）. Moreover, SBC increased the abundance of dominant bacterial phyla in soil, with Proteobacteria, Bacteroidota, and Cyanobacteria increasing most significantly. SBC decreased the diversity of soil microorganisms, but the decrease was insignificant （P≥0.05） compared with that in CK and BC. SEM analysis indicated that the available phosphorus, available potassium, organic matter, and soil pH were the key factors influencing Cd availability in soil, whereas organic matter and Cd availability were the key factors affecting the soil microbial community. Overall, SBC could stabilize Cd effectively and increase the abundance of dominant bacteria and has great potential in the remediation of Cd-contaminated soil.

Temperature, relative humidity and elderly type 2 diabetes mortality: A spatiotemporal analysis in Shandong, China.

BACKGROUND: The mortality of type 2 diabetes mellitus (T2DM) can be affected by environmental factors. However, few studies have explored the effects of environmental factors across diverse regions over time. Given the vulnerability observed in the elderly group in previous research, this research applied Bayesian spatiotemporal models to assess the associations in the elderly group. METHODS: Data on T2DM death in the elderly group (aged over 60 years old) at the county level were collected from the National Death Surveillance System between 1st January 2013 and 31st December 2019 in Shandong Province, China. A Bayesian spatiotemporal model was employed with the integrated Nested Laplace Approach to explore the associations between socio-environmental factors (i.e., temperatures, relative humidity, the Normalized Difference Vegetation Index (NDVI), particulate matter with a diameter of 2.5 µm or less (PM2.5) and gross domestic product (GDP)) and T2DM mortality. RESULTS: T2DM mortality in the elderly group was found to be associated with temperature and relative humidity (i.e., temperature: Relative Risk (RR) = 1.41, 95% Credible Interval (CI): 1.27-1.56; relative humidity: RR = 1.05, 95% CI:1.03-1.06), while no significant associations were found with NDVI, PM2.5 and GDP. In winter, significant impacts from temperature (RR = 1.18, 95% CI: 1.06-1.32) and relative humidity (RR = 0.94, 95% CI: 0.89-0.99) were found. Structured and unstructured spatial effects, temporal trends and space-time interactions were considered in the model. CONCLUSIONS: Higher mean temperatures and relative humidities increased the risk of elderly T2DM mortality in Shandong Province. However, a higher humidity level decreased the T2DM mortality risk in winter in Shandong Province. This research indicated that the spatiotemporal method could be a useful tool to assess the impact of socio-environmental factors on health by combining the spatial and temporal effects.

Long-term climate warming and extreme cold events driving ecological shifts in a deep oligo-mesotrophic lake.

Deep lakes are critical for freshwater storage, yet they are struggling against major ecological issues from climate change and nutrient pollution. A comprehensive understanding of internal feedback mechanisms is crucial for regulating nutrients in these lakes. A five-year study was conducted on the diatom community and environment in Lake Fuxian, China's largest deep freshwater lake, which is becoming eutrophic. The results indicate a shift in the diatom community from a stable state dominated by a single species to a rapid seasonal fluctuation, and there is a significant increase in diatom biomass. Specifically, stable stratification and low nutrient concentrations are limiting the growth of diatom biomass and maintaining the dominance of Cyclotella. Nutrients in the hypolimnion were replenished in the epilimnion during the extreme cold of winter, triggering a shift in the diatom community. This shift may imply that future climate change will exacerbate the positive feedback of hypoxia-nutrient release of algal blooms, potentially triggering a regime shift in the ecosystem of the entire lake. This study underscores the fact that climate change alters the internal feedback mechanisms of deep lakes, reducing ecosystem stability, and provides a scientific basis for further clarification of protection measures for deep lakes.

Impacts of heatwaves on type 2 diabetes mortality in China: a comparative analysis between coastal and inland cities.

The impacts of extreme temperatures on diabetes have been explored in previous studies. However, it is unknown whether the impacts of heatwaves appear variations between inland and coastal regions. This study aims to quantify the associations between heat exposure and type 2 diabetes mellitus (T2DM) deaths in two cities with different climate features in Shandong Province, China. We used a case-crossover design by quasi-Poisson generalized additive regression with a distributed lag model with lag 2 weeks, controlling for relative humidity, the concentration of air pollution particles with a diameter of 2.5 µm or less (PM2.5), and seasonality. The wet- bulb temperature (Tw) was used to measure the heat stress of the heatwaves. A significant association between heatwaves and T2DM deaths was only found in the coastal city (Qingdao) at the lag of 2 weeks at the lowest Tw = 14℃ (relative risk (RR) = 1.49, 95% confidence interval (CI): 1.11-2.02; women: RR = 1.51, 95% CI: 1.02-2.24; elderly: RR = 1.50, 95% CI: 1.08-2.09). The lag-specific effects were significant associated with Tw at lag of 1 week at the lowest Tw = 14℃ (RR = 1.14, 95% CI: 1.03-1.26; women: RR = 1.15, 95% CI: 1.01-1.31; elderly: RR = 1.15, 95% CI: 1.03-1.28). However, no significant association was found in Jian city. The research suggested that Tw was significantly associated with T2DM mortality in the coastal city during heatwaves on T2DM mortality. Future strategies should be implemented with considering socio-environmental contexts in regions.

Accumulation and risk assessment of mercury in soil as influenced by mercury mining/smelting in Tongren, Southwest China.

To investigate the influence of mercury (Hg) mining/smelting on the surrounding soil environment, ninety soil samples were collected around Hg mining/smelting areas in Tongren city, Guizhou Province, Southwest China. The total mercury (THg), methylmercury (MeHg), bioavailability and fractions of Hg in the soil and their potential risk were evaluated. The results showed that Hg mining/smelting significantly increased the soil pH and decreased the soil organic matter content (p < 0.05). The THg content in the surrounding soil was much higher than that at the control site, with almost all the samples exceeding the national standard in China (3.4 mg/kg, GB15618-2018). Similarly, the concentrations of MeHg (0.09-2.74 µg/kg) and bioavailable Hg (0.64-62.94 µg/kg) in these soil samples were also significantly higher than those in the control site. However, the MeHg/THg ratio was significantly lower in mining/smelting influenced soils (0.01-0.68%) than in control soils (0.60-3.72%). Fraction analysis revealed that residual (RES-Hg) and organic matter-bounded (OM-Hg) Hg accounted for more than 50% of the THg. Ecological risk assessment revealed that the potential ecological risk for most of the Hg mining/smelting-influenced soils (30.16 ≤ Er ≤ 2280.02) were higher than those at the control site (15.12 ≤ Er ≤ 27.1). In addition, these Hg mining/smelting-influenced soils posed acceptable noncarcinogenic risks to adults (except for two soil samples), with hazard indices (HIs) ranging from 0.04 to 1.11 and a mean HI of 0.44. However, children suffer serious noncarcinogenic risks, with HIs ranging from 0.34 to 7.43 and a mean HI of 3.10.

Enrichment and immobilization of heavy metal ions from wastewater by nanocellulose/carbon dots-derived composite.

Heavy metal ions (HMIs) have been widely applied in various industries because of their excellent physicochemical properties. However, their discharging without appropriate treatment brought about serious pollution problems. So it is desirable but challenging to rapidly and completely clean up these toxic pollutants from water, especially utilizing environmentally friendly and naturally rich biomass materials. In this work, we prepared nanocellulose/carbon dots/magnesium hydroxide (CCMg) ternary composite using cotton via a simple hydrothermal method. The removal mechanism towards Cd2+ and Cu2+ was investigated using a combination of experimental techniques and density functional theory calculations. CCMg shows a good ability to remove HMIs. It is realized that the interaction between each component of CCMg and cadmium nitrate is mainly of hydrogen/dative bonds. Cadmium nitrate is preferentially enriched by the Mg(OH)2 moiety, proved by calculated thermodynamics, interfacial interactions and charges. After transformation, the cadmium carbonate precipitate is fixed on the surface by nanocellulose (NC) via chemical coupling; and of interest is that copper ion precipitates in the form of basic sulfate. Due to its high adsorption effect and simple recovery operation, CCMg is having a wide range of application prospects as a water treatment agent.

The declines of heterogeneity and stability in diatom communities are associated with human activity.

Anthropogenic forcing caused the biodiversity loss and stability decline of communities. There is still controversy over whether the decline in biodiversity will lead to a decrease in community stability. The stability of biological communities is related to both biodiversity and structure, and this paper aims to reveal the human impacts on diatom communities' biodiversity and structure. We studied the richness, ß-diversity and network distance of diatom communities in Qinghai-Xizang, Yunnan-Sichuan and Lower Yangtze River Basin, China through empirical dataset and simulation method. The results showed that the diatoms richness in the Qinghai-Xizang and the Yunnan-Sichuan region was lower and the network distance was higher than that of the Lower Yangtze River Basin. ß-diversity in the Lower Yangtze River Basin was the lowest and the diatom network distance responds negatively to human population densities in China. The simulation showed that the network distance kept constant during random species loss, and declined while specialist species were lost or replaced by generalist species. The results suggested diatom communities' homogeneity and stability decline were associated with human activities. Human impacts may cause biodiversity loss targeted to specialist species or no biodiversity loss while generalist species replace those specialist species. This study showed that how diversity changes determined ecological stability depends on the type of species changes.

Revealing the hidden burden for lake management: the sediment phosphorus storage pools in Eastern Plain Lake Zone, China.

As one of the essential components in ecosystems, lakes play a major role in the global phosphorus (P) cycle. It is helpful for further understanding of the inside lake P geochemical cycle to research P pollution and storage in lakes, which is of positive significance for lake eutrophication restoration. In this study, we investigated the total phosphorus concentrations (TPC) of water and sediments from 37 lakes in the Eastern Plain Lake Zone (EPL) of China, evaluated the P pollution degree of lakes, and estimated P storage in lake sediments with quantitative data of lake area and number. The results indicate that the total phosphorus concentrations of water (TPCW) and total phosphorus concentrations of the surface sediments (0-1 cm, TPCSS) in EPL were high, the mean values were 0.11 mg·L-1 and 869.85 mg·kg-1 respectively, with obvious differences between urban and rural areas, as well as between different river basins. Over half (56.76% and 70.27% respectively) of the lakes reached severe pollution levels in water and surface sediments. There were 16224 lakes (> 0.01 km2) with a total area of 21662.37 km2 in the EPL, and the P storage in the lake sediments (0-30 cm) was about 4.87 ± 2.08 Tg (1 Tg = 1 × 1012 g), accounting for about 2.74% of the basin soil. TPCW and TPCSS of lakes in the EPL were significantly positively correlated, may suggest a close nutrient cycling relationship between the lake water and the sediment. During periods of high winds and waves, the stored P in the top sediments in the EPL may continue to participate in the internal P geochemical cycle and migrate to the overlying water, posing a potential pollution hazard. Therefore, it is crucial to take into account the sediment P pools when formulating effective lake phosphorus management strategies.

Test and Development of a Specialized Pipeline for Ventilator Calibration.

A specialized Y-type pipeline is developed to replace medical disposable Y-type pipelines for ventilator calibration and to improve accuracy, providing a reference for improvement. According to the calibration specifications of ventilators, tests are performed on specialized pipelines and more to compare their data. Taking the tidal volume 400-mL detection point as an example, the calibration errors made by medical disposable pipelines and specialized pipelines are 6.2% and -0.8%, respectively, and the errors at other detection points are roughly the same. The accuracy of the specialized Y-type pipeline has significantly improved, reducing the impact of the pipeline on tidal volume calibration from more than 6% to less than 1%. The use of specialized pipelines can significantly improve the accuracy and qualification rate of calibration, reducing the consumption of disposable Y-type pipelines and thereby significantly reducing costs and increasing efficiency.

Impact of environmental factors on diabetes mortality: A comparison between inland and coastal areas.

BACKGROUND: Diabetes mortality varies between coastal and inland areas in Shandong Province, China. However, evidence about the reasons for this disparity is limited. We assume that distinct environmental conditions may contribute to the disparities in diabetes mortality patterns between coastal and inland areas. METHOD: Qingdao and Jinan were selected as typical coastal and inland cities in Shandong Province, respectively, with similar socioeconomic but different environmental characteristics. Data on diabetes deaths and environmental factors (i.e., temperature, relative humidity and air pollution particles with a diameter of 2.5 µm or less (PM2.5)) were collected from 2013 to 2020. Spatial kriging methods were used to estimate the aggregated diabetes mortality at the city level. A distributed lag non-linear model (DLNM) was used to quantify the possible cumulative and non-cumulative associations between environmental factors and diabetes mortality by age, sex and location. RESULTS: In the coastal city (Qingdao), the maximum cumulative relative risks (RRs) of temperature and PM2.5 associated with diabetes deaths were 2.54 (95 % confidence interval (CI): 1.25-5.15), and 1.17 (95 % CI: 1.01-1.37) respectively, at lag 1 week. In the inland city (Jinan), only temperature exhibited significant cumulative associations with diabetes deaths (RR = 1.54, 95 % CI: 1.07-2.23 at 29 °C). Lower relative humidity (22 %-45 %) had a lag-specific association with diabetes deaths in inland areas at lag 3 weeks (RR = 1.33, 95 % CI: 1.03-1.70 at 22 %). CONCLUSION: Despite the lower PM2.5 concentrations in the coastal location, diabetes mortality exhibited stronger links to environmental variables in the coastal city than in the inland city. These findings suggest that the control of air pollution could decrease the mortality burden of diabetes, even in the region with relatively good air quality. Additionally, the spatial estimation method is recommended to identify associations between environmental factors and diseases in studies with limited data.

Effects of vacuum microwave combined with freeze-drying on the physicochemical properties, phenolic compounds, and antioxidant capacity of pear fruit slices.

The effects of freeze-drying (FD), vacuum microwave drying after freeze-drying (FD-VMD), and freeze-drying after vacuum microwave drying (VMD-FD) on the physicochemical properties, phenolic compounds, and antioxidant capacity of pear fruit slices were investigated. The results showed that FD samples had the highest crispness value (116.30 N·sec) and the lowest volume shrinkage ratio value (5.48%). Compared to FD, the VMD-FD and FD-VMD methods could save drying time without affecting the color of dried samples. FD-VMD samples had the lowest rehydration capacity and maintained a homogeneous porous structure, while the VMD-FD samples had obvious collapse. Compared to VMD-FD samples, FD-VMD samples had higher contents of ascorbic acid (20.91 mg/100 g), total phenolic (7.62 mg/g), total anthocyanin (0.21 mg/g), and gallic acid (1.21 µg/g). Moreover, FD-VMD samples showed the highest antioxidant capacity as evaluated by the 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging capacity, and H2 O2 content. Compared with FD and VMD-FD, FD-VMD was most effective in maintaining better quality and shortening drying time of pear fruit slices. These findings suggested that FD-VMD might be a promising drying technique in the fruits and vegetable processing industries.

Microwave bag cooking affects the quality, glucosinolates content and hydrolysate production of broccoli florets.

Appropriate processing and cooking technologies can effectively improve the content of bioactive compounds in vegetables. The effects of microwave bag cooking on broccoli floret quality attributes, glucosinolates (GLSs) content and hydrolysate production were investigated in this study. Microwave bag cooking not only preserved the color of the florets, but also enhanced the total phenolic and flavonoid content, as well as total chlorophyll and ascorbic acid content. Furthermore, the majority of the microorganisms were inactivated, and the structure of the florets was greatly destroyed, thereby increasing antioxidant capacity and promoting the release of GLSs and myrosinase activity in the florets. Moreover, microwave bag cooking significantly enriched the sulforaphane (SFN) and indole-3-carbinol (I3C) production of broccoli florets in the meantime, demonstrating that it was a convenient and quick cooking option to satisfy the requirements of modern consumers.

Performance and emissions of a diesel engine fueled by coal-based diesel fuels and their blends with polyoxymethylene dimethyl ethers.

The objective of this study was to investigate the performance and emissions of a diesel engine fueled by coal-based diesel fuels and their blends with oxygenated fuel polyoxymethylene dimethyl ethers (PODEn). First, coal-based Fischer-Tropsch (FT) diesel fuel was blended with hydrogenated diesel fuel at three volume ratios of 40%/60%, 50%/50%, and 60%/40%, denoted as T6W4, T5W5, and T4W6, respectively. Then, PODEn were added into the T4W6 fuel with the volume ratios of 10%, 20%, and 30% to evaluate its effects on the performance and emissions of a coal-based diesel engine. The results showed that the output torques and powers of the three coal-based diesel blends were slightly lower than those of the petroleum diesel fuel. The brake specific fuel consumption (BSFC) of the coal-based diesel fuels was almost the same as that of the petroleum diesel fuel. The brake thermal efficiencies (BTE) of the coal-based diesel blends were slightly lower than that of the petroleum diesel fuel, and the maximum reduction was 1.59%. The pollutant emissions of T5W5 were the closest to those of petroleum diesel fuel. The nitrogen oxides (NOx) emissions of T4W6 were lower, with a maximum decrease of 11.18% compared with the petroleum diesel. The carbon monoxide (CO) and hydrocarbon (HC) emissions of T6W4 were the highest, with maximum increases of 36.79% and 29.05%, respectively. The smoke emissions of T4W6 and T6W4 were higher than those of petroleum diesel fuel. Adding PODEn into T4W6 lowered the engine power and torque but increased the BSFC and BTE. The output torque and power of the diesel engine were further reduced when PODEn were blended with T4W6, with the maximum reductions of 17.76% and 16.96%, respectively. With an increase in the PODEn blending ratio, BSFC and BTE increased gradually, and the maximum increase in the BTE was 1.57%. Blending PODEn with the fuel effectively improved the emission characteristics of the coal-based diesel fuels. The NOx emissions increased slightly, but the emissions of HC, CO, and smoke were reduced significantly, with maximum reductions of 24.42%, 31.67%, and 82.35%, respectively.

Age- and Gender-Specific Differences in the Seasonal Distribution of Diabetes Mortality in Shandong, China: A Spatial Analysis.

Diabetes mortality in Shandong is higher than the national average in China. This study first explored diabetes mortality variation spatially at the county/district level among adults aged over 30 years in terms of age and gender, specifically by season. Daily diabetes mortality data were collected from 31 mortality surveillance points across Shandong Province in 2014. A geographic information system, spatial kriging interpolation and a spatial clustering method were used to examine the spatial patterns of diabetes mortality at the county/district level by season. Sensitivity analysis was conducted using diabetes mortality data from 10 mortality surveillance points from 2011 to 2020. As a result, the total diabetes mortality in eastern counties/districts was the highest (relative risk (RR) of cluster: 1.58, p = 0.00) across the whole province. For subgroups, women had higher mortality (16.84/100,000) than men (12.15/100,000), people aged over 75 years were the most vulnerable (93.91/100,000) and the highest-risk season was winter. However, the mortality differences between winter and summer were smaller in eastern and coastal regions than in other regions for all gender- and age-specific groups. The findings provide further evidence for early warning and precision preventative strategies for diabetes mortality in different regions of Shandong Province. Future research is required to identify the risk factors for diabetes and understand the differences in the social and environmental contexts.

Lactobacillus rhamnosus GG normalizes gut dysmotility induced by environmental pollutants via affecting serotonin level in zebrafish larvae.

Intestinal peristalsis is essential for gastrointestinal function, which could maintain the appropriate progression and digestion of food and reduce bacterial aggregation through mixing function. Even though certain ingredients of foodstuff are known to increase or decrease intestinal peristalsis, the role of environmental pollutants on intestinal peristalsis is relatively unknown. Therefore, the effects of four typical environmental pollutants (oxytetracycline, arsenic, polychlorinated biphenyls and chlorpyrifos) on intestinal peristalsis in the zebrafish model and then tested the recovery effect of the constipation-resistant probiotic. The results showed that 4-day environmental pollutants exposures on the zebrafish embryos at 1 day post fertilization clearly decreased the intestinal peristalsis through decreasing the serotonin (5-HT) production and down-regulating the expression of key genes involved in 5-HT synthesis. Pollutants-evoked change of gut motility could be normalized in the presence of Lactobacillus rhamnosus GG (LGG) via increasing 5-HT secretion. Exogenous 5-hydroxytryptophan (100 µg/L) could also rescue the dysfunction of gut motility in pollutants-treated zebrfish. The data identified that LGG normalized disorder of intestinal peristalsis induced by environmental pollutants through increasing 5-HT level. The stimulant effect of LGG on peristalsis may be associated with 5-HT system, which could provide references for the application of probiotics in regulation of gut dysmotility.

Exploring psychological resilience of entrepreneurial college students for post-pandemic pedagogy: The mediating role of self-efficacy.

The psychological impact of the COVID-19 epidemic on college students is an important topic. With the entry of the post-epidemic era, how universities can better improve students' psychological resilience in teaching is the research topic of this article. In the form of a questionnaire survey, some entrepreneurial college students investigated the loneliness and psychological resilience of college students after the outbreak of the epidemic and explored the role of self-efficacy in it. The data is collected online through cooperation with an entrepreneurial event, and the participating students are asked for background information such as colleges, grade, and majors. After collecting this information, they answered a series of simplified scale questions about loneliness, self-efficacy, and psychological resilience. In the end, a total of 200 questionnaires from different universities were collected, and the structural equation model was used to explore the role of self-efficacy. The results show that: loneliness has a significant negative effect on Self-efficacy, ß = -0.292, p < 0.001; Self-efficacy has a significant positive effect on psychological resilience, ß = 0.556, p < 0.0 01; loneliness has a significant negative effect on psychological resilience, ß = -0.244, p < 0.01. Self-Efficacy has a significant intermediate effect in loneliness and psychological resilience, with an effective value of -0.111 and p < 0.01. The results show that this exploratory survey finds it important to provide targeted personal self-efficacy improvement activities for college students with a strong sense of loneliness and to combine school and family education organically to help college students form a healthy and upward mentality to better cope with the unknown and troubles caused by the epidemic, which will help improve the psychological resilience of college students in the epidemic.

Pickering emulsion hydrogel based on alginate-gellan gum with carboxymethyl chitosan as a pH-responsive controlled release delivery system.

Pickering emulsion hydrogels (PEHs) were developed as a pH-responsive, controlled-release delivery system to address the limitations of Pickering emulsions in some harsh processing or gastrointestinal conditions. Specifically, the PEHs were fabricated based on alginate and various concentrations of gellan gum (GG) with carboxymethyl chitosan (CMCS) matrix. The encapsulation efficiency (EE), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) results proved the successful encapsulation. Furthermore, the hydrogels remained stable in the presence of destabilizing ions (Na+ or phosphate ions) and high osmotic pressure mediums. The texture profile analysis (TPA) characteristics and Young's modulus of the 0.8 % GG (w/v) PEHs were superior to the others. The PEHs prevented the emulsions from being released at pH 2.0, while the emulsions were entirely released at pH 7.4 in vitro, with the rate of release controlled by CMCS and the degree by GG concentration. This work facilitates the delivery of Pickering emulsions with excellent stability and pH-responsive controlled release for hydrophobic actives in food applications.

Taurine Treatment Alleviates Intestinal Mucositis Induced by 5-Fluorouracil in Mice.

Taurine (Tau), a ß-amino acid, exists in red goji fruit (Lycium barbarum L.). It exerts many cellular physiological functions such as anti-inflammation and oxidation resistance. The chemotherapy drug 5-fluorouracil (5FU) can cause intestinal mucositis. However, current therapeutic approaches for mucositis have limited efficacy and are associated with various side effects. It is still unknown whether Tau can alleviate intestinal mucositis. This study aimed to investigate the protective effect of the Tau in a mucositis mouse model and elucidate the underlying molecular mechanisms. The intestinal mucositis symptoms were alleviated by the Tau administration as evidenced by decreased body weight loss, histopathological score, oxidative stress, and improved glutathione (GSH). The Tau supplementation strengthened intestinal epithelial tight junction and reduced serum lipopolysaccharide (LPS) levels in intestinal mucositis mice. Moreover, the 5FU-induced inflammatory responses were alleviated by Tau treatment via the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) and nuclear factor kappa-B/inducible nitric oxide synthase (NF-κB/iNOS) signaling pathway. Tau administration modulated short chain fatty acids (SCFAs) in the colon of mice. The results indicated that the Tau might be a new dietary strategy for intestinal mucositis caused by 5FU.

Encapsulation of indole-3-carbinol in Pickering emulsions stabilized by OSA-modified high amylose corn starch: Preparation, characterization and storage stability properties.

The stability of hydrophobic bioactive compound indole-3-carbinol (I3C) is a challenge for application. In this work, Pickering emulsions were prepared to encapsulate I3C. As the emulsifier, high amylose corn starch was pretreated by acid hydrolysis, afterwards modified by different concentrations of octenyl succinic anhydride (OSA), and their emulsions were evaluated. The XRD, SEM and FTIR results indicated the successful modification. ζ-potential, mean droplet size and emulsification index (EI) of the emulsions confirmed that modified starch with a higher degree of substitution (DS) was more effective for enhancing the storage stability. The results of encapsulation efficiency (EE) and retention degree of I3C after 14 d also proved the assumption. Moreover, the Pickering emulsions protected I3C against ultraviolet light and achieved controlled release in vitro. The food-grade Pickering emulsion loading I3C is promising to be used as a nutrient or dietary supplement for food applications.