Association between interleukin 17A gene polymorphisms and risk of coronary artery disease.

Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide, and genetic factors contribute to the development of this disease. We conducted a case-control study to assess the association between interleukin 17A (IL17A) rs2275913 and rs3748067 polymorphisms and development of CAD. A total of 372 CAD patients and 372 healthy controls were recruited in our investigation between January 2013 and December 2014. Genotyping of IL17A rs2275913 and rs3748067 was carried out using polymerase chain reaction combined with restriction fragment length polymorphism. Logistic regression analysis revealed that CC [odds ratio (OR) = 3.81, 95% confidence interval (CI) = 2.11-7.16] and TC+CC (OR = 1.54, 95%CI = 1.11-2.14) rs3748067 genotypes were associated with an increased risk of CAD compared to the TT variant. Individuals carrying the TC+CC genotype were more likely to have a higher risk of CAD if they were smokers, with an adjusted OR (and 95%CI) of 2.20 (1.31-3.71). In conclusion, we suggest that the CC and TC+CC genotypes of rs3748067 are connected with increased risk of CAD in comparison to the wide-type genotype, particularly in smokers.

Investigation into the association between NLRP3 gene polymorphisms and susceptibility to type 2 diabetes mellitus.

We conducted a case-control study to investigate the role of three common polymorphisms (rs10754558, rs7512998, and rs12137901) of the gene NLR family, pyrin domain containing 3 (NLRP3) in the development of type 2 diabetes mellitus (T2DM). Between May 2013 and May 2014, 385 patients with T2DM and 401 control subjects were enrolled in our study. Genotyping of the three NLRP3 polymorphisms of interest was performed by polymerase chain reaction-restriction fragment length polymorphism. Unconditional logistic regression analyses showed that individuals carrying GG and GC+GG rs10754558 genotypes were at significantly increased risk of T2DM, with adjusted odds ratios (and 95% confidence intervals) of 1.81 (1.16-2.83) and 1.40 (1.04-1.88), respectively. In conclusion, we propose that the NLRP3 rs10754558 polymorphism contributes to the development of T2DM, but that rs7512998 and rs12137901 variants are not associated with susceptibility to this disease.