ABSTRACT
BACKGROUND: To investigate the function of miR-191-5p in lung adenocarcinoma and its possible mechanism. METHODS: QRT-PCR was adopted for the detection of the expression levels of miR-191-5p and SATB1 (HGNC: 10541). The effects of miR-191-5p and SATB1 on cell proliferation and migration were examined through the CCK-8 and Transwell assays. Subsequently, the binding relationships between miR-191-5p and SATB1 were confirmed by dual-luciferase reporter gene assay. Finally, the potential mechanisms of action of miR-191-5p were explored through a serious of in vivo and in vitro experiments. RESULTS: Lung adenocarcinoma patients had a notably lower expression level of miR-191-5p than controls, patients with metastasis had a lower level than those without metastasis, and the level in patients with lung adenocarcinoma in stage III-IV was lower than that in patients with lung adenocarcinoma in stage I-II. Overexpression of miR-191-5p repressed the migration and proliferation of lung cancer A549/H1650 cells. According to the reporter gene assay, miR-191-5p could bind to SATB1. Besides, SATB1 was significantly overexpressed in cancer tissues of patients with lung adenocarcinoma, and SATB1 overexpression accelerated the migration and proliferation of A549/H1650 cells and reversed inhibition on cell migration and proliferation by miR-191-5p. CONCLUSION: Overexpression of miR-191-5p is capable of blocking the migration and proliferation of lung cancer cells, and its mechanism may be through targeting SATB1 thus downregulating Wnt signaling.
Subject(s)
Adenocarcinoma of Lung/genetics , Lung Neoplasms/genetics , Matrix Attachment Region Binding Proteins/genetics , MicroRNAs/genetics , A549 Cells , Adenocarcinoma of Lung/metabolism , Adenocarcinoma of Lung/pathology , Animals , Cell Movement , Cell Proliferation , Gene Expression Regulation, Neoplastic , Humans , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , Male , Matrix Attachment Region Binding Proteins/metabolism , Mice , Mice, Inbred BALB C , Mice, Nude , MicroRNAs/metabolism , Wnt Signaling PathwayABSTRACT
OBJECTIVE: To detect the expression of AT-rich sequence-binding protein (SATB1) mRNA in non-small cell lung cancer (NSCLC) and explore the role of SATB1 in the development of NSCLC. METHODS: The total RNA was extracted from NSCLC tissues and normal lung tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of SATB1 mRNA these tissues. RESULTS: The expression of SATB1 mRNA was 13-fold higher in NSCLC tissues than in normal lung tissues (P<0.001), and in metastatic and nonmetastatic NSCLC, the expression was 23.63 and 5.57 folds that in normal lung tissues, respectively. CONCLUSION: SATB1 mRNA expression might be associated with the development and lymph node metastasis of NSCLC and may potentially used as an indicator for predicting the prognosis of NSCLC.
