Coiling an aneurysm in the twig-like MCA via a Marathon microcatheter: A case report.

INTRODUCTION AND IMPORTANCE: The twig-like middle cerebral artery (MCA) is rare and has a plexiform network of small vessels that replaces the MCA trunk, with a prevalence ranging from 0.11 %-1.17 %. It can be associated with an aneurysm. A Marathon microcatheter may be an alternative for coiling procedures. However, this technique has rarely been reported. PRESENTATION OF CASE: A 63-year-old man suffered from headaches. The neurological examination results were unremarkable. Angiography revealed a twig-like MCA with an unruptured aneurysm. Due to stenosis of the aneurysm neck, a Marathon microcatheter was used to successfully reach the aneurysm, and coiling was completed with Chinese Visee coils. Postoperatively, the patient recovered uneventfully. Due to the lack of ischemic changes in the right hemisphere, the patient was kept under follow-up observation. At the 8-month follow-up by telephone, the patient was healthy. CLINICAL DISCUSSION: Aneurysms in the twig-like MCA may have stenosis of the aneurysm neck, and routine microcatheters used to deliver coils are often too thick to catheterize the aneurysm neck. The Marathon microcatheter has a distal inner diameter (ID) of 0.013, and as an alternative for coiling procedures, it may be soft enough to thin enough to go into the aneurysm. However, it can be used in the delivery of certain coils. CONCLUSION: Aneurysms in the twig-like MCA are difficult to catheterize with the routine microcatheters used to deliver coils. A Marathon microcatheter may be used to perform the coiling procedure. However, only certain coils that match the Marathon microcatheter can be chosen.

Endovascular treatment for V3 segment direct vertebral-venous fistulas: A report of two cases and a literature review.

INTRODUCTION AND IMPORTANCE: Direct vertebrovertebral fistulas (VVFs) involving the V3 segment of the vertebral artery (VA) are rare. Endovascular treatment (EVT) can be used to obliterate these VVFs. CASE PRESENTATION: Case 1 was a 30-year-old male with limb weakness. He had grade V muscle strength in his limbs. Angiography confirmed a low-flow direct VVF of the V3 segment. The right VA was well developed. Coiling of the VVF and its parent VA obliterated the VVF. The vertebrobasilar arteries had sufficient blood from the right VA. Postoperatively, the patient recovered well. Case 2 was a 51-year-old male with headache and weakness of the limbs. He had grade IV muscle strength in his limbs. Angiography revealed a high-flow direct VVF in the V3 segment. The left VA was well developed. Coiling of the VVF and its parent VA obliterated the VVF. The vertebrobasilar arteries had sufficient blood from the left VA. Postoperatively, the patient recovered well. CLINICAL DISCUSSION: Direct VVF of the V3 segment is difficult to treat, and EVT, including reconstructive and deconstructive approaches, can be an effective treatment option. According to this case report and literature review, reconstructive EVT is ideal; however, it is difficult to perform. Currently, deconstructive EVT may be the mainstream option for treating direct VVF of the V3 segment. CONCLUSION: In certain cases where the contralateral VA is well developed, coiling the fistula and the parent VA is still an effective treatment.

Preparation of magnetic amphiphilic resin microspheres via the one-step polymerization method and extraction of four glucocorticoids for HPLC-MS analysis.

Amphiphilic materials can be used for sample preparation of chromatography or mass spectrometry. Amphiphilic materials with magnetic properties in combination with magnetic suction devices allow for automated sample preparation. However, conventional synthesis methods are cumbersome and not suitable for the mass production of the material. In this study, a micro-suspension polymerization method was developed to synthesize magnetic amphiphilic resin microspheres (MARMs), providing new ideas for the preparation of amphiphilic microspheres. MARMs with particle sizes ranging from 3 to 6 µm were successfully prepared, with BET surface area up to 653.2 m2/g. A magnetic solid-phase extraction method based on MARM-5 was developed for the extraction of four glucocorticoids including Cortisone, Hydrocortisone, Cortodoxone, and Corticosterone. This method had a very short adsorption time of 0.5 min and a total extraction time of only 13 min. The limit of detection for the four glucocorticoids ranged from 0.22 to 0.82 ng/L. There was a good linear relationship between sample concentration and peak area in the range of 25∼500 ng/L. Relative recovery of 98 %∼108 % and internal standard normalized matrix effect factors of 95∼114 % were obtained, and the relative standard deviation was between 2.3 % and 6.3 %. The MARMs would be used as excellent solid extraction material for glucocorticoids.

The effect of the occipital sinus on the torcular Herophili and neighboring structures.

Objective: The occipital sinus (OS) has many variations. It is unknown whether OS can change the angioarchitecture of torcular Herophili. Therefore, we performed magnetic resonance venography (MRV) in a cohort of Han Chinese individuals to determine whether OS can change the angioarchitecture of torcular Herophili. Methods: Participants were divided into a normal OS group and a hyperplastic OS group. Parameters of the OS and torcular Herophili and its neighboring structures were recorded. Statistical analysis was used to determine the effects of OS on torcular Herophili and neighboring structures. Results: One hundred forty-five healthy participants were enrolled. One hundred patients were in the normal OS group, and 45 were in the hyperplastic OS group. In the normal OS group, the diameters at the transverse sinus (TS) origin were 5.8 ± 2.3 mm on the left side and 7.5 ± 2.2 mm on the right side. In the hyperplastic OS group, the diameters at the TS origin were 6.0 ± 2.1 mm on the left side and 7.0 ± 2.7 mm on the right side. Fenestration was observed in 33% of the torculars in the normal OS group and 6.7% of the torculars in the hyperplastic group. An unpaired t-test revealed a significant difference between bilateral TSs in the normal OS group (P < 0.05) but no difference in the hyperplastic OS group. The chi-square test revealed a significant difference in torcular Herophili fenestration between the normal and hyperplastic OS groups (P < 0.05). Conclusions: Hyperplastic OS makes bilateral TSs equal in diameter and weakens the predominance of the right TS. A hyperplastic OS reduces the occurrence of torcular Herophili fenestration.

Morphometric CT angiographic study of the SSS and its adjacent structures: A comparative analysis between elderly and nonelderly individuals of a Han Chinese population.

Objective: The superior sagittal sinus (SSS) is an important structure, but few studies have analyzed it using computed tomography angiography (CTA). Methods: This study was performed to examine the angiographic anatomy of the SSS and its adjacent structures using CTA in Han Chinese participants. According to age, participants were divided into elderly and nonelderly groups. The parameters of the SSS and adjacent structures were measured, recorded and analyzed statistically. Results: A total of 500 Han Chinese participants were enrolled in this study, including 346 in the elderly group and 154 in the nonelderly group. In the elderly group, regarding inferior sagittal sinus (ISS) development, 187 ISSs were absent, 85 were visible, and 74 were clear. In the nonelderly group, 62 ISSs were absent, 54 were visible, and 38 were clear. In the elderly group, the Rolandic bridging vein diameter was 3.6 ± 0.8 mm; in the nonelderly group, the diameter was 3.9 ± 1.1 mm. The statistical results showed a difference in ISS development between the elderly and nonelderly groups (P < 0.05). The relationship of age with ISS development was assessed using linear regression analysis, and the results indicated that ISS became gradually occluded with age (P < 0.05). The statistical results also showed a difference in the Rolandic bridging vein diameter between the elderly and nonelderly groups (P < 0.05). The relationship of age with the Rolandic bridging vein diameter was assessed using linear regression analysis, and the results indicated that the Rolandic bridging vein tended to become thinner with age (P < 0.05). Conclusion: This study found that more ISSs may become occluded and that the Rolandic bridging vein may become thinner with age. Other parameters of the SSS and its adjacent structures may not be affected by aging. In addition, our study also provided normal CTA parameters of the SSS and its adjacent structures in Han Chinese people.

Embolization of cerebellar pial arteriovenous fistula presented with congestive edema of the brainstem and cervical cord.

Cerebellar pial arteriovenous fistula (PAVF) in adults presenting with congestive edema of the brainstem and cervical cord has rarely been reported. Here, we report such a case. A 59-year-old man presented with progressive weakness and numbness of the limbs and hiccups for 6 months. On physical examination, his limbs had grade III muscle strength, and he was unable to stand or walk. He also had occasional incontinence and retention. There was a positive Babinski sign in both lower limbs. Below the neck, he had hypoesthesia of the skin. Magnetic resonance imaging (MRI) showed edema of the brainstem and upper cervical cord. Digital subtraction angiography (DSA) confirmed there was a cerebellar nongalenic-type PAVF lesion; the feeding arteries were the branches of the posterior inferior cerebellar artery (PICA); and the draining vein was a single cortical vein that ultimately entered the venous systems of the brainstem and upper cervical cord. Casting Onyx-18 via the PICA obliterated the PAVF. Postoperatively, the patient recovered gradually. Two and a half months later, he could walk using a crutch and had no incontinence. Six-month follow-up MRI and DSA revealed recession of the edema of the brainstem and cervical cord and no recurrence of this PAVF. One year later, he was nearly normal. In conclusion and extremely rarely, cerebellar nongalenic-type PAVF can present with congestive edema of the brainstem and high cervical cord. This treatment by Onyx-18 embolization can resolve PAVF.

Endovascular treatment of middle cerebral artery aneurysms: current status and future prospects.

Middle cerebral artery (MCA) aneurysms are complex and widely distributed throughout the course of the MCA. Various types of aneurysms can occur in the MCA. Ruptured as well as unruptured MCA aneurysms may require treatment to avoid bleeding or rebleeding. Currently, clipping is regarded as the first-line choice for the treatment of MCA aneurysms. However, endovascular treatment (EVT) is emerging as an alternative treatment in selected cases. EVT techniques vary. Therefore, it is necessary to review EVT for MCA aneurysms. In this review, the following issues were discussed: MCA anatomy and anomalies, classifications of MCA aneurysms, the natural history of MCA aneurysms, EVT status and principle, deployments of traditional coiling techniques and flow diverters (FDs), and deployments and prospects of intrasaccular flow disruptors and stent-like devices. According to the review and our experience, traditional coiling EVT is still the preferred therapy for most MCA aneurysms. FD deployment can be used in selective MCA aneurysms. Parent artery occlusion (PAO) can be used to treat distal MCA aneurysms. In addition, new devices can be used to treat MCA aneurysms, such as intrasaccular flow disruptors and stent-like devices. In general, EVT is gaining popularity as an alternative treatment option; however, there is still a lack of evidence regarding EVT, and longer-term data are not currently available for most EVT devices.

Endovascular treatment of a supraclinoid internal carotid artery fenestration aneurysm: A case report and literature review.

Supraclinoid internal carotid artery (ICA) fenestration aneurysm is rare. Except for open surgery, endovascular treatment (EVT) is considered an alternative for such an aneurysm. However, experience with this procedure is lacking. Therefore, we reported such a case. A 61-year-old woman suffered subarachnoid hemorrhage. Digital subtracted angiography (DSA) showed bilateral middle cerebral artery (MCA) aneurysms and a saccular aneurysm associated with fenestration of the supraclinoid ICA. Two MCA aneurysms were treated with single coiling, and the supraclinoid ICA fenestration aneurysm was coiled under stent assistance. The postoperative recovery was uneventful. At this time, a literature review was performed on the role of EVT in supraclinoid ICA fenestration aneurysms. A total of 13 supraclinoid ICA fenestration aneurysms treated by EVT in 11 cases, including our case, were obtained. After EVT, good outcomes were obtained in all cases. To our knowledge, this is the first study to review the role of EVT for supraclinoid ICA fenestration aneurysms. Our case report and literature review indicated that EVT for such aneurysms may be feasible and act as a therapeutic alternative.

Transarterial embolization of dural arteriovenous fistula of the inferior petrosal sinus: A report of two cases.

Dural arteriovenous fistula (DAVF) of the inferior petrosal sinus (IPS) is very unusual. Endovascular embolization is a good option for the treatment of DAVF. Until now, DAVFs of the IPS have only been reported sporadically. We reported two such cases. Case 1 was a 48-year-old man with headache and diplopia. Angiography confirmed a DAVF of the distal IPS, mainly supplied by the occipital artery (OA), and the IPS was occluded, which retrogradely drained into the cavernous sinus (CS) and then into the cortical vein. The DAVF in case 1 was embolized completely via the OA to cast Onyx-18. Case 2 was a 69-year-old female who had red and swollen eyes. Angiography confirmed a DAVF of the proximal IPS, mainly supplied by the accessory meningeal artery (AMA), which drained into the CS and then into the ophthalmic vein, and the IPS was occluded. The DAVF in case 2 was embolized completely via the AMA to cast Onyx-18. After treatment, these two patients had uneventful recoveries. In our report, these two cases indicated that the DAVFs of the proximal and distal IPS shared different origins of feeding arteries. When the IPS is occluded, the transarterial approach via the main feeder, such as OA and AMA, can be feasible to cure the DAVF of the IPS.

Wearable Continuous Blood Pressure Monitoring Devices Based on Pulse Wave Transit Time and Pulse Arrival Time: A Review.

Continuous blood pressure (BP) monitoring is of great significance for the real-time monitoring and early prevention of cardiovascular diseases. Recently, wearable BP monitoring devices have made great progress in the development of daily BP monitoring because they adapt to long-term and high-comfort wear requirements. However, the research and development of wearable continuous BP monitoring devices still face great challenges such as obvious motion noise and slow dynamic response speeds. The pulse wave transit time method which is combined with photoplethysmography (PPG) waves and electrocardiogram (ECG) waves for continuous BP monitoring has received wide attention due to its advantages in terms of excellent dynamic response characteristics and high accuracy. Here, we review the recent state-of-art wearable continuous BP monitoring devices and related technology based on the pulse wave transit time; their measuring principles, design methods, preparation processes, and properties are analyzed in detail. In addition, the potential development directions and challenges of wearable continuous BP monitoring devices based on the pulse wave transit time method are discussed.

Parent artery occlusion for ruptured aneurysms in moyamoya vessels or on collaterals.

Background: Aneurysms in moyamoya vessels or on collaterals are difficult to treat. Parent artery occlusion (PAO) via endovascular treatment (EVT) is often the last resort, but the safety and efficacy of this approach need to be evaluated. Materials and methods: A retrospective study was performed on patients admitted to our hospital who were diagnosed with unilateral or bilateral moyamoya disease (MMD) associated with ruptured aneurysms in moyamoya vessels or on collaterals. These aneurysms were treated with PAO, and the clinical outcome was recorded. Results: Eleven patients were aged 54.7 ± 10.4 years, and six patients were male (54.5%, 6/11). The aneurysms in 11 patients were single and ruptured, and the average size was 2.7 ± 0.6 mm. Three (27.3%, 3/11) aneurysms were located at the distal anterior choroidal artery, 3 (27.3%, 3/11) were at the distal lenticulostriate artery, 3 (27.3%, 3/11) were at the P2-3 segment of the posterior cerebral artery, 1 (9.1%, 1/11) was at the P4-5 segment of the posterior cerebral artery, and 1 was at the transdural location of the middle meningeal artery. Among the 11 aneurysms, PAO by coiling was performed on 7 (63.6%, 7/11), and Onyx casting was performed on 4 (36.4%, 4/11). Of 11 patients, 2 (18.2%, 2/11) suffered intraoperative hemorrhagic complications. During follow-up, all patients had good outcomes with a modified Rankin scale score of 0-2. Conclusion: As a last resort, the application of PAO with coiling or casting Onyx for ruptured aneurysms in moyamoya vessels or on collaterals may be safe with an acceptable clinical outcome. However, patients with MMD may not always achieve expected health outcomes, and PAO for the aneurysm can bring only temporary relief.

CTA Study of Ruptured Aneurysms of the Posterior Communicating Artery.

Background: Only a few reported studies have used computed tomography angiography (CTA) to image ruptured aneurysms at the junction of the internal carotid artery (ICA) and posterior communicating artery (PcomA) in the context of the adjacent arteries. Therefore, we performed such a study using a GE Workstation. Methods: The parameters of each aneurysm and its adjacent arteries were measured. Then, statistical assessments were performed to compare the parameters of the aneurysm side and the lesion-free (control) side. Results: Sixty-three patients were included in this study. The average age was 62.1 ± 11.0 years, and the ratio of males to females was 0.8 : 1. The measurement results showed that the mean aneurysmal height was 5.2 ± 2.3 mm, the mean width was 4.7 ± 2.2 mm, and the mean neck width was 4.5 ± 1.9 mm. On the aneurysm side, the intradural ICA diameter was 4.34 ± 0.90 mm, and the diameter of the ICA at its termination was 3.55 ± 0.72 mm. A fetal-type PcomA was found in 52.4% of aneurysms. The other measured parameters were also provided. Statistical results showed that the height of the aneurysm was larger than the width (P < 0.05). The intradural ICA diameter, the ICA diameter at termination, the intradural ICA length, and the angle between the ICA and PcomA were larger in the aneurysm group than in the control group (P < 0.05). Conclusions: This CTA study showed that the ruptured PcomA aneurysm was often wide-necked, nonspherical, and approximately 5 mm in size. In the presence of a ruptured PcomA aneurysm, the affected intradural ICA became thicker and longer than the contralateral control ICA, and the aneurysm significantly reduced the angle between the ICA and the PcomA.

Endovascular treatment of main trunk aneurysms in the residual anterior circulation in moyamoya disease.

BACKGROUND: Main trunk aneurysms in the residual anterior circulation in moyamoya disease (MMD) are uncommon, and in such cases, endovascular treatment (EVT) is a good choice. MATERIALS AND METHODS: A retrospective study was performed on 35 consecutive patients admitted to our hospital who were diagnosed with MMD and main trunk aneurysms in the residual anterior circulation and were treated with EVT. RESULT: The 35 patients were aged 38-77 years (mean, 56.1 ± 8.8 years) and included 17 females (48.6%, 17/35). There were 29 cases (82.9%, 29/35) of hemorrhagic onset. In 35 patients, there were 38 main trunk aneurysms in the residual anterior circulation. Thirty-eight aneurysms underwent coiling; among them, coiling with stent assistance was used in the treatment of 6 (15.8%, 6/38) aneurysms. The immediate modified Raymond-Roy classification (MRRC) was grade I for all aneurysms. Among 35 patients, intraoperative bleeding occurred in 2 (5.7%, 2/35) patients. After EVT, immediate hemiplegia occurred in 4 (11.4%, 4/35) patients, and immediate coma occurred in 1 (2.9%, 1/35) patient. At discharge, in 35 patients, the Glasgow Outcome Scale (GOS) was five in 88.6% of them. Of 35 patients, 68.6% had follow-up data, GOS was five in 87.5% patients, and the MRRC was grade I for all aneurysms in the follow-up angiography. CONCLUSION: For main trunk aneurysms in the residual anterior circulation in MMD, although EVT was accompanied by potential ischemic and hemorrhagic complications, which should be considered, EVT can still offer an acceptable prognosis in more than 85% of patients.

Transfemoral trans-facial vein-superior ophthalmic vein to embolize cavernous sinus dural arteriovenous fistulas.

Cavernous sinus dural arteriovenous fistula (CS-DAVF) is an abnormal communication between the CS and dural arteries from the internal carotid artery and external carotid artery. CS-DAVFs are not uncommon. The preferred treatment for most CS-DAVFs is transvenous embolization (TVE), which can achieve a high cure rate with few complications. The trans-inferior petrous sinus (IPS) route from the internal jugular vein to the CS is the favorite and most direct route to perform TVE in the great majority of CS-DAVFs. However, when the trans-IPS route fails and if the facial vein (FV) is patent and dilated, transfemoral trans-FV-superior ophthalmic vein (SOV) embolization of the CS-DAVF can be attempted. However, the transfemoral trans-FV-SOV route to embolize CS-DAVFs is often challenging, and there is insufficient knowledge about it. Therefore, an updated review of the transfemoral trans-FV-SOV route to embolize CS-DAVFs is necessary, and this review includes our experience. The images in this review are from our institute without the dispute of copyright. Issues regarding the transfemoral trans-FV-SOV route to embolize CS-DAV were discussed, including the FV anatomy and variation, various TVE routes to access CS-DAVF, the procedure of the transfemoral trans-FV-SOV route to embolize CS-DAVF, difficulty, and solution of the transfemoral trans-FV-SOV route to embolize CS-DAVF, and complications and prognosis of transfemoral trans-FV-SOV to embolize CS-DAVF. By reviewing the transfemoral trans-FV-SOV route to embolize CS-DAVFs, we found that this route provides a valuable alternative to the other transvenous routes. A good prognosis can be obtained with the transfemoral trans-FV-SOV route to embolize CS-DAVFs in select cases.

Preparation of Bisphenol-A and Polydimethylsiloxane (PDMS) Block Copolycarbonates by Melt Polycondensation: Effects of PDMS Chain Length on Conversion and Miscibility.

This study aimed to improve polydimethylsiloxane (PDMS) conversion in the preparation of polycarbonate (PC)-polydimethylsiloxane (PDMS) copolymer through melt polycondensation. We examined the transesterification process of PDMS with diphenyl carbonate (DPC) and its copolymerization products with bisphenol-A (BPA) for different chain lengths of PDMS. The key factors affecting PDMS conversion were investigated. Results showed that long-chain PDMS required a higher critical transesterification level (38.6%) to improve miscibility with DPC. During polycondensation, side reactions were more prone to occur when the equilibrium transesterification level of long-chain PDMS was lower. PDMS conversion was also lower when more short-chain PDMS was fed. Increasing the chain length of PDMS also reduced PDMS conversion. Notably, increasing the amount of KOH can significantly improve PDMS conversion throughout the polycondensation stage by increasing the equilibrium transesterification level of long-chain PDMS, thereby inhibiting the occurrence of side reactions.

Using geochemistry to identify and quantify the sources, distribution, and fluxes of baseflow to an intermittent river impacted by climate change: The upper Wimmera River, southeast Australia.

Documenting the distribution, sources and fluxes of baseflow discharge into rivers is important for their management and for maintaining ecosystem health. This study uses major ion geochemistry, 222Rn, and 3H to differentiate between the input of low-salinity near-river waters (bank storage and return waters and/or interflow) and regional groundwater in an intermittent river from southeast Australia that is undergoing long-term changes in flow resulting from climate change. Baseflow discharge calculated by 222Rn mass balance was up to 1.3 m3/m/day in the high flow period in July 2019 and up to 0.1 m3/m/day at low flow conditions in November 2019. The distribution of 222Rn activities implies higher baseflow fluxes in the upper and middle reaches that have relatively steep topography and higher hydraulic gradients. The lower reaches received less baseflow due to subdued topography and fine-grained sediments. The observation that Cl concentrations did not increase uniformly downstream, however, implies that much of the baseflow may comprise bank return flow or interflow. This conclusion is also consistent with water mass balance calculations and the observation that 3H activities (1.85-3.00 TU) in the river were higher than in the groundwater (<0.45 TU). Intermittent streams are likely to be less well connected to regional groundwater, and thus near-river water stores will be more important in sustaining streamflow during dry periods than regional groundwater. These rivers and their ecosystems may be less susceptible to the impacts of groundwater extraction and the near-river waters will provide a buffer zone from potentially contaminated regional groundwater. However, these near river stores are vulnerable to short-term climate variability, and changes to flow regimes resulting from climate change may significantly impact water supplies and ecosystem health.

Adaptive Complex Variational Mode Decomposition for Micro-Motion Signal Processing Applications.

In order to suppress the strong clutter component and separate the effective fretting component from narrow-band radar echo, a method based on complex variational mode decomposition (CVMD) is proposed in this paper. The CVMD is extended from variational mode decomposition (VMD), which is a recently introduced technique for adaptive signal decomposition, limited to only dealing with the real signal. Thus, the VMD is extended from the real domain to the complex domain firstly. Then, the optimal effective order of singular value is obtained by singular value decomposition (SVD) to solve the problem of under-decomposition or over-decomposition caused by unreasonable choice of decomposition layer, it is more accurate than detrended fluctuation analysis (DFA) and empirical mode decomposition (EMD). Finally, the strongly correlated modes and weakly correlated modes are judged by calculating the Mahalanobis distance between the band-limited intrinsic mode functions (BLIMFs) and the original signal, which is more robust than the correlation judgment methods such as computing cross-correlation, Euclidean distance, Bhattachryya distance and Hausdorff distance. After the weak correlation modes are eliminated, the signal is reconstructed locally, and the separation of the micro-motion signal is realized. The experimental results show that the proposed method can filter out the strong clutter component and the fuselage component from radar echo more effectively than the local mean decomposition (LMD), empirical mode decomposition and moving target indicator (MTI) filter.

Diagnostic accuracy of the Xpert MTB/RIF assay for bone and joint tuberculosis using tissue specimens.

OBJECTIVES: To evaluate the diagnostic accuracy of the Xpert MTB/RIF assay for bone and joint tuberculosis (BJTB) using tissue specimens, and to compare the diagnostic accuracy of different types of tissue specimens. METHODS: This study involved 242 patients admitted with suspected BJTB between May 2018 and March 2020. The Xpert MTB/RIF assay was performed on surgically excised tissue. Diagnostic accuracy of the Xpert MTB/RIF assay was evaluated by culture, histopathology and a composite reference standard (CRS). RESULTS: One hundred and seventy-five patients were excluded (91 based on the exclusion criteria, and 84 as pus specimens were used instead of tissue specimens). Of the 67 patients enrolled, 14 were confirmed as BJTB, 20 as probable BJTB, 11 as possible BJTB, and 22 as non-BJTB. Using culture as the reference standard, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and area under the curve (AUC) of the Xpert MTB/RIF assay were 92.9% (88.2-97.6%), 62.3% (56.1-68.5%), 39.4% (33.2-45.6%), 97.1% (92.3-100.0%), 2.464 (1.077-3.851), 0.114 (0.025-0.203) and 0.776 (0.654-0.897), respectively. When histopathology was used as the reference standard, the Xpert MTB/RIF assay had sensitivity, specificity, PPV, NPV, PLR, NLR and AUC of 79.3% (73.5-85.1%), 73.7% (67.8-79.6%), 69.7% (63.8-75.6%), 82.4% (76.5-88.3%), 3.015 (1.184-4.846), 0.281 (0.141-0.421) and 0.765 (0.646-0.884), respectively. Sensitivity, specificity, PPV, NPV, PLR, NLR and AUC obtained when using CRS as the reference were 73.3% (67.9-78.7%), 100.0% (100.0-100.0%), 100.0% (100.0-100.0%), 64.7% (58.5-70.9%), +∞, 0.267 (0.129-0.405) and 0.867 (0.781-0.952), respectively. Tissue samples were classified, and the positive rate of the Xpert MTB/RIF assay for BJTB using granulation tissue specimens was found to be significantly higher than that for caseous necrotic tissue, sequestrum and other necrotic connective tissues (P < 0.05). CONCLUSION: The Xpert MTB/RIF assay showed high sensitivity and specificity for the diagnosis of BJTB from tissue specimens.

Metabolism: A Novel Shared Link between Diabetes Mellitus and Alzheimer's Disease.

As a chronic metabolic disease, diabetes mellitus (DM) is broadly characterized by elevated levels of blood glucose. Novel epidemiological studies demonstrate that some diabetic patients have an increased risk of developing dementia compared with healthy individuals. Alzheimer's disease (AD) is the most frequent cause of dementia and leads to major progressive deficits in memory and cognitive function. Multiple studies have identified an increased risk for AD in some diabetic populations, but it is still unclear which diabetic patients will develop dementia and which biological characteristics can predict cognitive decline. Although few mechanistic metabolic studies have shown clear pathophysiological links between DM and AD, there are several plausible ways this may occur. Since AD has many characteristics in common with impaired insulin signaling pathways, AD can be regarded as a metabolic disease. We conclude from the published literature that the body's diabetic status under certain circumstances such as metabolic abnormalities can increase the incidence of AD by affecting glucose transport to the brain and reducing glucose metabolism. Furthermore, due to its plentiful lipid content and high energy requirement, the brain's metabolism places great demands on mitochondria. Thus, the brain may be more susceptible to oxidative damage than the rest of the body. Emerging evidence suggests that both oxidative stress and mitochondrial dysfunction are related to amyloid-ß (Aß) pathology. Protein changes in the unfolded protein response or endoplasmic reticulum stress can regulate Aß production and are closely associated with tau protein pathology. Altogether, metabolic disorders including glucose/lipid metabolism, oxidative stress, mitochondrial dysfunction, and protein changes caused by DM are associated with an impaired insulin signal pathway. These metabolic factors could increase the prevalence of AD in diabetic patients via the promotion of Aß pathology.

A comparative study on the value of Xpert MTB/RIF and T-SPOT.TB tests in the diagnosis of bone and joint tuberculosis.

BACKGROUND: We evaluated the application value of Xpert MTB/RIF and T-SPOT.TB in the diagnosis of bone and joint tuberculosis. METHODS: One hundred two patients with suspected bone and joint tuberculosis (BJTB) were admitted to Zhengzhou Orthopaedics Hospital, Henan, China from April 2018 to February 2019. The Xpert MTB/RIF and T-SPOT.TB tests were performed using pus specimens and peripheral blood, respectively. The diagnostic performance of Xpert MTB/RIF and T-SPOT.TB tests was evaluated on the basis of the composite reference standard (CRS). RESULT: A Total of 73 suspected BJTB were enrolled and categorized, including 12 confirmed BJTB, 27 probable BJTB and 34 non-BJTB. When CRS was used as the reference, the specificity, PPV and NLR values of the Xpert MTB/RIF assay were significantly higher than those of the T-SPOT.TB assay (97.1% vs. 82.4%, p < 0.05; 96.7% vs. 85.4%, p < 0.05; 0.26 vs. 0.12, p < 0.05). However, the sensitivity, NPV and PLR values of the T-SPOT. TB assay were significantly higher than those of the Xpert MTB/RIF assay (89.7% vs. 74.4%, p < 0.05; 87.5% vs. 76.7%, p < 0.05; 5.08 vs. 2.52, p < 0.05). The AUCs from Xpert MTB/RIF and T-SPOT. TB tests were 0.857 and 0.860, respectively. However, the difference was not statistically significant. The Xpert MTB/RIF and T-SPOT. TB tests demonstrated medium concordance in diagnosing BJTB. CONCLUSION: The sensitivity of T-SPOT.TB test combined with the specificity of Xpert MTB/RIF not only shorten the time of diagnosis but also improve the accuracy of diagnosis of BJTB and reduce the misdiagnosis rate. Therefore, they are useful for early diagnosis of BJTB.