ABSTRACT
BACKGROUND: Postoperative urinary tract infection is a common complication that not only significantly prolongs the hospital stay and amplifies the economic burden on patients, but also affects their quality of life and prognosis. This study aimed to investigate risk factors and distribution of pathogenic bacteria in urinary tract infections among bladder cancer patients who underwent cutaneous ureterostomy following radical cystectomy. METHODS: A total of 137 bladder cancer patients, who underwent cutaneous ureterostomy after radical cystectomy at our hospital from November 2018 to October 2022, were enrolled in this retrospective study. Univariate and multivariate logistic regression analyses were employed to investigate the risk factors associated with postoperative urinary tract infection and the distribution of pathogenic bacteria among the infected patients. RESULTS: The results of both univariate and multivariate analyses confirmed that age, proficiency in ostomy knowledge, frequency of ureteral stent tube replacement, ureteral stent tube dislodgement, urine immersion at the outer end of the ureteral stent tube, and the interval of ostomy bag replacement were independent risk factors for urinary tract infection after radical cystectomy and cutaneous ureterostomy in bladder cancer patients. A total of 55 pathogenic bacteria were isolated from 52 patients with infections. Predominantly, these were gram-negative bacteria (34 strains, 61.8%), with Proteus mirabilis having the highest proportion. CONCLUSION: Urinary tract infections after radical cystectomy and cutaneous ureterostomy predominantly involve gram-negative bacteria. This is correlated with factors such as the age of bladder cancer patients, the level of nursing education, the duration of ureteral stent tubes and ostomy bag usage, as well as issues related to impaired urine drainage.
Subject(s)
Cystectomy , Postoperative Complications , Ureterostomy , Urinary Bladder Neoplasms , Urinary Tract Infections , Humans , Urinary Bladder Neoplasms/surgery , Male , Female , Cystectomy/adverse effects , Risk Factors , Urinary Tract Infections/microbiology , Urinary Tract Infections/etiology , Retrospective Studies , Aged , Middle Aged , Postoperative Complications/microbiology , Aged, 80 and over , AdultABSTRACT
Recently, electroencephalographic (EEG) emotion recognition attract attention in the field of human-computer interaction (HCI). However, most of the existing EEG emotion datasets primarily consist of data from normal human subjects. To enhance diversity, this study aims to collect EEG signals from 30 hearing-impaired subjects while they watch video clips displaying six different emotions (happiness, inspiration, neutral, anger, fear, and sadness). The frequency domain feature matrix of EEG signals, which comprise power spectral density (PSD) and differential entropy (DE), were up-sampled using cubic spline interpolation to capture the correlation among different channels. To select emotion representation information from both global and localized brain regions, a novel method called Shifted EEG Channel Transformer (SECT) was proposed. The SECT method consists of two layers: the first layer utilizes the traditional channel Transformer (CT) structure to process information from global brain regions, while the second layer acquires localized information from centrally symmetrical and reorganized brain regions by shifted channel Transformer (S-CT). We conducted a subject-dependent experiment, and the accuracy of the PSD and DE features reached 82.51% and 84.76%, respectively, for the six kinds of emotion classification. Moreover, subject-independent experiments were conducted on a public dataset, yielding accuracies of 85.43% (3-classification, SEED), 66.83% (2-classification on Valence, DEAP), and 65.31% (2-classification on Arouse, DEAP), respectively.
Subject(s)
Brain , Emotions , Humans , Electroencephalography/methods , FearABSTRACT
In recent years, there has been a growing interest in the study of emotion recognition through electroencephalogram (EEG) signals. One particular group of interest are individuals with hearing impairments, who may have a bias towards certain types of information when communicating with those in their environment. To address this, our study collected EEG signals from both hearing-impaired and non-hearing-impaired subjects while they viewed pictures of emotional faces for emotion recognition. Four kinds of feature matrices, symmetry difference, and symmetry quotient based on original signal and differential entropy (DE) were constructed, respectively, to extract the spatial domain information. The multi-axis self-attention classification model was proposed, which consists of local attention and global attention, combining the attention model with convolution through a novel architectural element for feature classification. Three-classification (positive, neutral, negative) and five-classification (happy, neutral, sad, angry, fearful) tasks of emotion recognition were carried out. The experimental results show that the proposed method is superior to the original feature method, and the multi-feature fusion achieved a good effect in both hearing-impaired and non-hearing-impaired subjects. The average classification accuracy for hearing-impaired subjects and non-hearing-impaired subjects was 70.2% (three-classification) and 50.15% (five-classification), and 72.05% (three-classification) and 51.53% (five-classification), respectively. In addition, by exploring the brain topography of different emotions, we found that the discriminative brain regions of the hearing-impaired subjects were also distributed in the parietal lobe, unlike those of the non-hearing-impaired subjects.
Subject(s)
Brain , Emotions , Humans , Emotions/physiology , Brain/physiology , Electroencephalography/methods , Recognition, Psychology , FearABSTRACT
2-Bromo-4, 6-dinitroaniline (BDNA) is a widespread azo-dye-related hazardous pollutant. However, its reported adverse effects are limited to mutagenicity, genotoxicity, endocrine disruption, and reproductive toxicity. We systematically assessed the hepatotoxicity of BDNA exposure via pathological and biochemical examinations and explored the underlying mechanisms via integrative multi-omics analyses of the transcriptome, metabolome, and microbiome in rats. After 28 days of oral administration, compared with the control group, 100 mg/kg BDNA significantly triggered hepatotoxicity, upregulated toxicity indicators (e.g., HSI, ALT, and ARG1), and induced systemic inflammation (e.g., G-CSF, MIP-2, RANTES, and VEGF), dyslipidemia (e.g., TC and TG), and bile acid (BA) synthesis (e.g., CA, GCA, and GDCA). Transcriptomic and metabolomic analyses revealed broad perturbations in gene transcripts and metabolites involved in the representative pathways of liver inflammation (e.g., Hmox1, Spi1, L-methionine, valproic acid, and choline), steatosis (e.g., Nr0b2, Cyp1a1, Cyp1a2, Dusp1, Plin3, arachidonic acid, linoleic acid, and palmitic acid), and cholestasis (e.g., FXR/Nr1h4, Cdkn1a, Cyp7a1, and bilirubin). Microbiome analysis revealed reduced relative abundances of beneficial gut microbial taxa (e.g., Ruminococcaceae and Akkermansia muciniphila), which further contributed to the inflammatory response, lipid accumulation, and BA synthesis in the enterohepatic circulation. The observed effect concentrations here were comparable to the highly contaminated wastewaters, showcasing BDNA's hepatotoxic effects at environmentally relevant concentrations. These results shed light on the biomolecular mechanism and important role of the gut-liver axis underpinning BDNA-induced cholestatic liver disorders in vivo.
Subject(s)
Chemical and Drug Induced Liver Injury , Cholestasis , Rats , Animals , Multiomics , Liver/metabolism , Cholestasis/chemically induced , Cholestasis/metabolism , Cholestasis/pathology , Chemical and Drug Induced Liver Injury/metabolism , Inflammation/metabolism , Bile Acids and Salts/adverse effects , Bile Acids and Salts/metabolismABSTRACT
We present the design and performances of a broadband 1 × 2 mode-independent thermo-optic (TO) switch based on Mach-Zehnder interferometer (MZI) with multimode interferometer (MMI). The MZI adopts a Y-branch structure as the 3-dB power splitter and a MMI as the coupler, which are designed to be insensitive to the guided modes. By optimizing the structural parameters of the waveguides, mode-independent transmission and switching functions for E11 and E12 modes can be implemented in the C + L band, and the mode content of the outputs is the same as the mode content of the inputs. We proved the working principle of our design based on polymer platform, which was fabricated by using ultraviolet lithography and wet-etching methods. The transmission characteristics for E11 and E12 modes were also analyzed. With the driving power of 5.9â mW, the measured extinction ratios of the switch for E11 and E12 modes are larger than 13.3â dB and 13.1â dB, respectively, over a wavelength range of 1530â nm to 1610â nm. The insertion losses of the device are 11.7â dB and 14.2â dB for E11 and E12 modes, respectively, at 1550â nm wavelength. The switching times of the device are less than 840 µs. The presented mode-independent switch can be applied in reconfigurable mode-division multiplexing systems.
ABSTRACT
Mounting evidence has shown that ambient PM2.5 exposure is closely associated with the development of obesity, and adipose tissue represents an important endocrine target for PM2.5. In this study, the 3T3-L1 preadipocyte differentiation model was employed to comprehensively explore the adipogenic potential of PM2.5. After 8 days of PM2.5 exposure, adipocyte fatty acid uptake and lipid accumulation were significantly increased, and adipogenic differentiation of 3T3-L1 cells was promoted in a concentration-dependent manner. Transcriptome and lipidome analyses revealed the systematic disruption of transcriptional and lipid profiling at 10 µg/mL PM2.5. Functional enrichment and visualized network analyses showed that the peroxisome proliferator-activated receptor (PPAR) pathway and the metabolism of glycerophospholipids, glycerolipids, and sphingolipids were most significantly affected during adipocyte differentiation. Reporter gene assays indicated that PPARγ was activated by PM2.5, demonstrating that PM2.5 promoted adipogenesis by activating PPARγ. The increased transcriptional and protein expressions of PPARγ and downstream adipogenesis-associated markers (e.g., Fabp4 and CD36) were further cross-validated using qRT-PCR and western blot. PM2.5-induced adipogenesis, PPARγ pathway activation, and lipid remodeling were significantly attenuated by the supplementation of a PPARγ antagonist (T0070907). Overall, this study yielded mechanistic insights into PM2.5-induced adipogenesis in vitro by identifying the potential biomolecular targets for the prevention of PM2.5-induced obesity and related metabolic diseases.
Subject(s)
Adipogenesis , PPAR gamma , Animals , Mice , PPAR gamma/genetics , PPAR gamma/metabolism , 3T3-L1 Cells , Lipids , Obesity , Cell DifferentiationABSTRACT
The mode-division multiplexing (MDM) is an effective technology with huge development potential to improve the transmission capacity of optical communication system by transmitting multiple modes simultaneously in a few-mode fiber. In traditional MDM technology, the fundamental modes of multiple channels are usually modulated by external individual arranged electro-optic modulators, and then multiplexed into the few-mode fiber or waveguide by a mode multiplexer. However, this is usually limited by large device footprint and high power consumption. Here, we report a mode-selective modulator and switch to individually modulate or switch the TE11, TE12 and TE21 modes in a few-mode waveguide (FMW) to overcome this limitation. Our method is based on the graphene-polymer hybrid platform with four graphene capacitors buried in different locations of the polymer FMW by utilizing the coplanar interaction between the capacitors and spatial modes. The TE11, TE12 and TE21 modes in the FMW can be modulated and switched separately or simultaneously by applying independent gate voltage to different graphene capacitor of the device. Our study is expected to make the selective management of the spatial modes in MDM transmission systems more flexible.
ABSTRACT
We report an ultra-broadband and compact TM-pass polarizer based on graphene-buried polymer waveguides. The characteristic parameters of the polarizer were carefully designed and optimized. The standard microfabrication processes were employed to fabricate the device. The presented polarizers exhibit high polarization-dependent transmission imposing a TE mode cutoff while leaving the TM mode almost unaffected. We experimentally demonstrated the polarizer that has an ultra-high extinction ratio of more than 22.9 dB and 41.9 dB for the monolayer graphene film placed on the surface of core layer and buried in the center of core layer, respectively, and as low insertion loss as ~4.0 dB for the TM mode with the bandwidth over 110 nm. The presented polarizer has the advantages of high extinction ratio, ultra-broadband, low cost, and easy integration with other polymer-based planar lightwave devices.
ABSTRACT
A polymer/silica hybrid 3D waveguide thermo-optic (TO) mode switch based on cascaded asymmetric directional couplers (ADCs) is theoretically designed and simulated, where the spatial modes of a few-mode silica waveguide can be switched to various single-mode polymer waveguides placed above the few-mode silica waveguide. A beam propagation method is employed to optimize the dimensional parameters of the mode switch to convert the LP11a and LP11b modes of the few-mode silica waveguide to the LP01 mode of two single-mode polymer waveguides using the cascaded ADC 1 and ADC 2. The coupling ratios are higher than 96.4% (93.4%) and 95.1% (92.8%) for the ADC 1 and ADC 2, respectively, under the TE (TM) polarization within the wavelength range from 1530 to 1570 nm, which shows good wavelength independence. Furthermore, the monolayer graphene is introduced as the heating electrode and buried on the surface of the polymer core to increase the heating efficiency and reduce the power consumption. The power consumption for ADC 1 and ADC 2 is 16.69 mW and 17.35 mW, respectively. Compared to the traditional TO switch with an aluminum (Al) heating electrode, the heating efficiency of the presented device can be improved by â¼30%. Moreover, the response speed of the TO mode switch with a 3D waveguide structure was also significantly improved. Compared to the device with Al electrodes, the introduced graphene electrodes can improve the switching speed of the device by â¼60%. The presented TO mode switch with its small size and easy integration should find applications in reconfigurable mode division multiplexing systems.
ABSTRACT
The exposome overhauls conventional environmental health impact research paradigms and provides a novel methodological framework that comprehensively addresses the complex, highly dynamic interplays of exogenous exposures, endogenous exposures, and modifiable factors in humans. Holistic assessments of the adverse health effects and systematic elucidation of the mechanisms underlying environmental exposures are major scientific challenges with widespread societal implications. However, to date, few studies have comprehensively and simultaneously measured airborne pollutant exposures and explored the associated biomarkers in susceptible healthy elderly subjects, potentially resulting in the suboptimal assessment and management of health risks. To demonstrate the exposome paradigm, we describe the rationale and design of a comprehensive biomarker and biomonitoring panel study to systematically explore the association between individual airborne exposure and adverse health outcomes. We used a combination of personal monitoring for airborne pollutants, extensive human biomonitoring, advanced omics analysis, confounding information, and statistical methods. We established an exploratory panel study of Biomarkers of Air Pollutant Exposure in Chinese people aged 60-69 years (China BAPE), which included 76 healthy residents from a representative community in Jinan City, Shandong Province. During the period between September 2018 and January 2019, we conducted prospective longitudinal monitoring with a 3-day assessment every month. This project: (1) leveraged advanced tools for personal airborne exposure monitoring (external exposures); (2) comprehensively characterized biological samples for exogenous and endogenous compounds (e.g., targeted and untargeted monitoring) and multi-omics scale measurements to explore potential biomarkers and putative toxicity pathways; and (3) systematically evaluated the relationships between personal exposure to air pollutants, and novel biomarkers of exposures and effects using exposome-wide association study approaches. These findings will contribute to our understanding of the mechanisms underlying the adverse health impacts of air pollution exposures and identify potential adverse clinical outcomes that can facilitate the development of effective prevention and targeted intervention techniques.
Subject(s)
Air Pollutants , Exposome , Aged , Air Pollutants/analysis , Air Pollutants/toxicity , Biomarkers , China , Environmental Exposure/analysis , Humans , Prospective StudiesABSTRACT
Ceramides are bioactive sphingolipids that have been implicated in insect development; however, their role in insect reproduction remains poorly understood. Here, we report the pivotal role of neutral ceramidase (NCER) in the female reproduction of the brown planthopper (BPH), Nilaparvata lugens (Stål), a significant pest in rice cultivation in Asia. LC-MS/MS demonstrated that, among different developmental stages of BPH, the levels of ceramides were highest in 1st instar nymphs and lowest in adults. The transcription of NCER was negatively correlated with the levels of ceramides at different developmental stages of BPH, in that the transcript levels of NCER were the highest, whereas ceramides levels were the lowest in BPH adults. Knocking down NCER through RNA interference (RNAi) increased the levels of ceramides in BPH females and ovaries, which resulted in a delay in oocyte maturation, a reduction in oviposition and egg hatching rate, as well as the production of vulnerable offspring. Transmission electron microscopy (TEM) analysis and TdT-mediated dUTP Nick-End Labeling (TUNEL) assays showed mitochondrial deficiency and apoptosis in NCER-deficient oocytes. Taken together, these results suggest that NCER plays a crucial role in female reproduction in BPH, likely by regulating the levels of ceramides.
ABSTRACT
If one uses data to identify the most likely epistatic interaction between two genetic units, and then tests if the identified interaction is associated with a phenotype, the nominal statistical evidence will be inflated. Corrections are available but computationally expensive for genome-wide studies. We provide a first-order correction that can be applied in practice with essentially no additional computational cost.
Subject(s)
Algorithms , Epistasis, Genetic , Genetic Association Studies , Models, Genetic , Polymorphism, Single Nucleotide , Computer Simulation , Genotype , Humans , Multifactor Dimensionality Reduction , Phenotype , Statistics as TopicABSTRACT
BACKGROUND: The patients with temporary stomas after anterior resection for rectal cancer may experience significant impact on their health outcomes, and hence continuing care is necessary and important for these patients. However, the effects of some single continuing care interventions remain unclear. Continuing care bundle may be an effective approach to address this uncertainty. OBJECTIVE: The aim of this study was to investigate the effects of an evidence-based continuing care bundle on selected health outcomes in patients with temporary stomas after anterior resection for rectal cancer. METHODS: This was a multicenter randomized controlled trial. A total of 124 patients with temporary stomas after anterior resection for rectal cancer were recruited from 4 general tertiary hospitals in Guangzhou, China, and were randomly assigned to a control group or an intervention group. Both groups received usual care, whereas the intervention group additionally received evidence-based continuing care bundle. Self-efficacy, quality of life, and stoma-related complications were collected at baseline and 4 and 12 weeks after surgery. Satisfaction and outcomes of stoma reversal were collected at the end of the observation. RESULTS: The intervention group had significantly improved the self-efficacy (F = 11.88, P = .001), quality of life (F = 17.99, P < .001) over time, satisfaction (t = 4.08, P < .001), and outcomes of stoma reversal (χ2 = 5.93, P = .015) and reduced the incidence of complications (P < .05). CONCLUSIONS: Evidence-based continuing care bundle can be an effective method to improve the health outcomes among these patients. IMPLICATION FOR PRACTICE: By using the evidence-based continuing care bundle, nurses can help these patients improve their health outcomes in stoma-specific nursing.
Subject(s)
Patient Care Bundles/methods , Patient Care Bundles/psychology , Quality of Life/psychology , Rectal Neoplasms/psychology , Self Efficacy , Surgical Stomas , Adult , China , Female , Humans , Male , Middle Aged , Outcome Assessment, Health Care , Rectal Neoplasms/surgery , Time FactorsABSTRACT
We investigated the microRNA172 (miR172)-mediated regulatory network for the perception of changes in external and endogenous signals to identify a universally applicable floral regulation system in ornamental plants, manipulation of which could be economically beneficial. Transgenic gloxinia plants, in which miR172 was either overexpressed or suppressed, were generated using Agrobacterium-mediated transformation. They were used to study the effect of altering the expression of this miRNA on time of flowering and to identify its mRNA target. Early or late flowering was observed in transgenic plants in which miR172 was overexpressed or suppressed, respectively. A full-length complementary DNA (cDNA) of gloxinia (Sinningia speciosa) APETALA2-like (SsAP2-like) was identified as a target of miR172. The altered expression levels of miR172 caused up- or down-regulation of SsAP2-like during flower development, which affected the time of flowering. Quantitative real-time reverse transcription PCR analysis of different gloxinia tissues revealed that the accumulation of SsAP2-like was negatively correlated with the expression of miR172a, whereas the expression pattern of miR172a was negatively correlated with that of miR156a. Our results suggest that transgenic manipulation of miR172 could be used as a universal strategy for regulating time of flowering in ornamental plants.
Subject(s)
Arabidopsis Proteins/genetics , Flowers/physiology , Homeodomain Proteins/genetics , Lamiales/physiology , MicroRNAs/genetics , Nuclear Proteins/genetics , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Cloning, Molecular , DNA, Complementary/metabolism , Flowers/genetics , Gene Expression Profiling , Gene Expression Regulation, Plant , Homeodomain Proteins/metabolism , Lamiales/genetics , MicroRNAs/metabolism , Nuclear Proteins/metabolism , Plants, Genetically Modified/genetics , Plants, Genetically Modified/physiology , Plasmids/metabolism , Polymerase Chain Reaction , TransgenesABSTRACT
We study computational approaches for detecting SNP-SNP interactions that are characterized by a set of "two-locus, two-allele, two-phenotype and complete-penetrance" disease models. We argue that existing methods, which use data to determine a best-fitting disease model for each pair of SNPs prior to screening, may be too greedy. We present a less greedy strategy which, for each given pair of SNPs, limits the number of candidate disease models to a set of prototypes determined a priori.
Subject(s)
Epistasis, Genetic , Models, Genetic , Alleles , Bipolar Disorder/genetics , Bipolar Disorder/pathology , Cluster Analysis , Genotype , Humans , Polymorphism, Single NucleotideABSTRACT
Gliotoxin is an important epipolythiodioxopiperazine, which was biosynthesized by the gli gene cluster in Aspergillus genus. However, the regulatory mechanism of gliotoxin biosynthesis remains unclear. In this study, a novel Zn2Cys6 transcription factor DcGliZ that is responsible for the regulation of gliotoxin biosynthesis from the deep-sea-derived fungus Dichotomomyces cejpii was identified. DcGliZ was expressed in Escherichia coli and effectively purified from inclusion bodies by refolding. Using electrophoretic mobility shift assay, we demonstrated that purified DcGliZ can bind to gliG, gliM, and gliN promoter regions in the gli cluster. Furthermore, the binding kinetics and affinity of DcGliZ protein with different promoters were measured by surface plasmon resonance assays, and the results demonstrated the significant interaction of DcGliZ with the gliG, gliM, and gliN promoters. These new findings would lay the foundation for the elucidation of future gliotoxin biosynthetic regulation mechanisms in D. cejpii.
Subject(s)
Fungi/genetics , Gliotoxin/biosynthesis , Multigene Family/genetics , Promoter Regions, Genetic/genetics , Transcription Factors/metabolism , Fungi/metabolism , Gliotoxin/chemistryABSTRACT
Ceramidases (CDases) are vital enzymes involved in the biosynthesis of sphingolipids, which are essential components of eukaryotic membranes. The function of these enzymes in insects, however, is poorly understood. We identified a neutral ceramidase (NlnCDase) from the brown planthopper, Nilaparvata lugens, one of the most destructive hemipteran pests of rice. The C12-ceramide was the most preferred substrate for the NlnCDase enzyme. The activity of the NlnCDase enzyme was highest in the neutral-pH range (pH 6.0). It was inhibited by EGTA, Cs+ and Fe2+, while stimulated by EDTA and Ca2+. Moreover, the NlnCDase has higher transcript level and activity in adults than in eggs and nymphs, and in the reproductive organs (ovaries and spermaries) than in other tissues (i.e. heads, thorax, legs, midguts), which suggested that the NlnCDase might be elevated to mediate developmental process. In addition, transcripts and activity of the NlnCDase were up-regulated under abiotic stresses including starvation, abnormal temperature, and insecticides, and biotic stress of resistant rice varieties. Knocking down NlnCDase by RNA interference increased female survival under starvation and temperature stresses, suggesting that NlnCDase might be involved in the stress response in N. lugens.
Subject(s)
Hemiptera/physiology , Neutral Ceramidase/genetics , Stress, Physiological , Animals , Enzyme Activation , Gene Expression Regulation , Gene Knockdown Techniques , Hemiptera/classification , Informatics/methods , Neutral Ceramidase/metabolism , Phylogeny , Protein Transport , Sequence Analysis, DNA , Stress, Physiological/geneticsABSTRACT
Changes in pig fertility have occurred as a result of domestication, but are not understood at the level of genetic variation. To identify variations potentially responsible for prolificacy, we sequenced the genomes of the highly prolific Taihu pig breed and four control breeds. Genes involved in embryogenesis and morphogenesis were targeted in the Taihu pig, consistent with the morphological differences observed between the Taihu pig and others during pregnancy. Additionally, excessive functional non-coding mutations have been specifically fixed or nearly fixed in the Taihu pig. We focused attention on an oestrogen response element (ERE) within the first intron of the bone morphogenetic protein receptor type-1B gene (BMPR1B) that overlaps with a known quantitative trait locus (QTL) for pig fecundity. Using 242 pigs from 30 different breeds, we confirmed that the genotype of the ERE was nearly fixed in the Taihu pig. ERE function was assessed by luciferase assays, examination of histological sections, chromatin immunoprecipitation, quantitative polymerase chain reactions, and western blots. The results suggest that the ERE may control pig prolificacy via the cis-regulation of BMPR1B expression. This study provides new insight into changes in reproductive performance and highlights the role of non-coding mutations in generating phenotypic diversity between breeds.
Subject(s)
Bone Morphogenetic Protein Receptors, Type I/genetics , Breeding , Genetic Variation , Litter Size/genetics , Sus scrofa/genetics , Animals , Bone Morphogenetic Protein Receptors, Type I/metabolism , Mutation , Whole Genome Sequencing/veterinaryABSTRACT
Microgravity has been previously demonstrated to induce skeletal muscle atrophy, loss of muscle force and disorders in myogenesis and metabolism. Current pharmacological strategies exhibit poor efficacy. Bu Zhong Yi Qi decoction (BZ) is a wellknown traditional Chinese medicine decoction used for myasthenia gravis. In the present study, its effect on unloading induced muscle atrophy was investigated. The mousetail suspension model was used to simulate weightlessness induced muscle atrophy. The results indicated that BZ could significantly protect muscles from simulated weightlessnessinduced atrophy. To elucidate the underlying mechanisms, drugCIPHERCS methods were introduced to predict its potential targets, significantly enriched pathways and biological processes. The results demonstrated that the calcium signaling pathway, citrate cycle, biosynthetic and lipid metabolic process are affected by BZ. Among the targets, nuclear receptor corepressor 1 (NCoR1) is one of the most important proteins involved in myogenesis and metabolism. The results indicated that BZ significantly downregulated NCoR 1 expression, and further induced muscle differentiation and metabolism by regulating NCoR1associated gene expression in vivo and in vitro. In summary, the present study indicated that may be effective in combating weightlessnessinduced muscle atrophy. Combined with bioinformatics, the underlying mechanism for this decoction was investigated, which provided an improved understanding of this decoction.
Subject(s)
Drugs, Chinese Herbal/therapeutic use , Muscular Atrophy/drug therapy , Muscular Atrophy/etiology , Weightlessness Simulation/adverse effects , Animals , Cell Line , Drug Delivery Systems , Drugs, Chinese Herbal/pharmacology , Gene Ontology , Male , Mice , Muscle Development/drug effects , Nuclear Receptor Co-Repressor 1/metabolism , Oxidation-Reduction , Signal Transduction/drug effectsABSTRACT
Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important predatory natural enemy of planthopper and leafhopper eggs in Asian rice paddy fields. Cyrtorhinus lividipennis is known to rely largely on herbivore-induced plant volatiles to identify eggs embedded in rice stem tissues for feeding and on pheromones for mating. However, exactly how C. lividipennis decode these chemical information is unclear. In most insects, the odorant-binding proteins (OBPs) and the chemosensory proteins (CSPs) are essential for seeking out food resources and mates. In this study, we identified 10 OBP and 5 CSP genes in C. lividipennis and investigated their expression patterns in various tissues of adult males and females by quantitative real-time PCR (qRT-PCR). Six OBP genes (ClivOBP1, 2, 4, 6, 9, and 10) were mainly expressed in the male antennae, whereas three genes (ClivOBP3, ClivOBP7, and ClivOBP8) had high expression in the female antennae. ClivCSP1 was predominantly expressed in the male antennae. These findings suggest that most ClivOBPs and ClivCSPs are likely involved in food-searching behavior. The recognition of the pheromone molecules provides the basis for further functional studies on the chemoreception system of C. lividipennis.