ABSTRACT
Effects of extracellular proteases of Aspergillus ochraceus and Aspergillus terreus on plasma hemostasis proteins, consist of initiating the activation of prothrombin complex proteins, was detected. Was discovered, that A. ochraceus proteases have a direct influence on protein C and coagulation factor X, and A. terreus proteases causes their activation indirectly through kallikrein system stimulation. The ability of extracellular proteases of micromycetes activate prekallikrein in human blood plasma on the example of A. terreus was first demonstrated.
Subject(s)
Aspergillus ochraceus/enzymology , Blood Proteins/analysis , Hemostasis , Peptide Hydrolases/chemistry , Blood Proteins/chemistry , Factor X/analysis , Factor X/metabolism , Humans , Peptide Hydrolases/metabolism , Plasma/chemistry , Prekallikrein/analysis , Prekallikrein/chemistry , Prekallikrein/metabolism , Protein C/analysis , Protein C/metabolism , Substrate SpecificityABSTRACT
Screening of the genus Aspergillus micromycetes for secretion of extracellular proteolytic enzymes, capable of acting on human proteins of the hemostatic system, has been conducted. The ability of extracellular proteases of Aspergillus to cleave specific proteins of the hemostatic system chromogenic peptide substrates, as well as activate a series of proenzymes (protein C, factor X and prothrombin) has been found. The ability of extracellular proteases of micromycetes activate X factor of human blood plasma was first shown at the first time.