Cryptococcoma hipofisiario: un extraño caso de hipofisitis granulomatosa secundaria / Pituitary cryptococcoma: a strange case of secondary granulomatous hypophysitis

Resumen La hipofisitis es una afección con baja incidencia y prevalencia. Asimismo, las infecciones profundas por hongos en pacientes inmunocompetentes también re presentan un fenómeno infrecuente. Más raro aún es el caso que se describe a continuación, en donde se conjugan estos dos elementos mencionados, a saber: cryptococcoma hipofisario o hipofisitis granulomatosa causado por dicho patógeno en un huésped sin altera ción de la respuesta inmune. Luego de una búsqueda realizada en PubMed, existen limitados casos en la literatura médica de hipofisitis granulomatosa por Cryptococcus spp., que simuló por manifestaciones clínicas e imagenológicas un macro adenoma hipofisario. No encontramos informes en los que no haya evidencia de afectación del tejido meníngeo. La etiología micótica está escasamente descrita en las guías de referencia para hipofisitis y creemos que Cryp tococcus spp. debe ser tenido en cuenta en el diagnóstico diferencial de las hipofisitis granulomatosas secundarias dado que es un patógeno ubicuo y el tratamiento es sustancialmente diferente a otras entidades. Cobra mayor relevancia ante la tendencia actual al uso de glucocorticoides sistémicos a altas dosis para el tratamiento de la hipofisitis, que podría haber gene rado mayor daño de no haberse hecho el diagnóstico correcto.

Abstract Hypophysitis is a pathology with low incidence and prevalence. Likewise, deep fungal infections in immuno competent patients also represent a rare phenomenon. Even rarer is the case described below, where these two mentioned elements are combined, namely: pituitary cryptococcoma or granulomatous hypophysitis caused by said pathogen in a host without altered immune response. After research in PubMed, there are limited cases in the medical literature of granulomatous hypophysitis caused by Cryptococcus spp., which simulated a pituitary macroadenoma by clinical and imaging manifestations. We did not find reports in which there is no evidence of involvement of the meningeal tissue. The fungal etiology is scarcely described in the reference guidelines for hypophysitis and we believe that Cryptococcus spp. it should be taken into account in the differential diagnosis of secondary granuloma tous hypophysitis since it is a ubiquitous pathogen and the treatment is substantially different from other entities. It becomes more relevant given the current trend towards the use of high-dose systemic glucocorticoids for the treatment of hypophysitis, which could have generated greater damage if the correct diagnosis had not been made.

Plasmonic and Photothermal Effects of CuS Nanoparticles Biosynthesized from Acid Mine Drainage with Potential Drug Delivery Applications.

In this work, the plasmonic and photothermal effects of CuS nanoparticles biosynthesized from acid mine drainage (AMD) were studied. CuS were formed by delivering the H2S generated by a sulfidogenic bioreactor to an off-line system containing the AMD. The precipitates collected after contact for an hour were washed and physico-chemically characterized, showing a nanoparticle with a mean diameter of 33 nm, crystalline nature and semiconductor behavior with a direct band gap of 2.2 eV. Moreover, the CuS nanoparticles exhibited localized surface plasmonic resonance in the near infrared range, with a high absorption band centered at 973 nm of wavelength, which allowed an increase in the temperature of the surrounding media under irradiation. Finally, the cytotoxicity of the CuS nanoparticles as well as their potential use as part of drug delivery platforms were investigated.

Bio-recovery of CuS nanoparticles from the treatment of acid mine drainage with potential photocatalytic and antibacterial applications.

In the present work, CuS nanoparticles were biorecovered from a real acid mine drainage (AMD) and its photocatalytic and antibacterial activities were studied. CuS were formed by delivering biogenic H2S produced by a continuous sulfidogenic bioreactor to an off-line vessel containing the AMD. The main physico-chemical properties of CuS nanoparticles were analyzed by UV-vis spectroscopy, TEM, FE-SEM, XRD and XPS. Moreover, its photocatalytic activity on the photodegradation of organic dyes in water and its antibacterial activity against several bacterial strains were studied and compared with CuS nanoparticles synthetized from a CuSO4 aqueous solution based on the same synthesis method. CuS nanoparticles from the real AMD showed similar physico-chemical properties and photocatalytic and antibacterial activities in comparison to CuS nanoparticles formed with the copper solutions. These results open the way to recover valorous CuS nanoparticles from AMD with potential industrial applications using a metal bioremediation process based on sulfidogenic bioreactors.

Carcinoma adrenocortical en mujeres en edad fértil / Adrenocortical carcinoma in women of reproductive age

Resumen Los carcinomas adrenocorticales son tumores infre cuentes, habitualmente hiperfuncionantes y con una supervivencia global pobre. La edad frecuente de pre sentación se describe en adultos entre 40 a 60 años, con predominio en sexo femenino. Se presentan dos casos inusuales de carcinoma adrenal diagnosticados en mujeres en edad fértil. El primero de ellos se descubrió en el segundo trimestre de gestación, con un cuadro de hipercortisolismo y lesión adrenal localizada, que resolvió con resección completa hacia la semana 20. En el segundo, la paciente debutó con manifestaciones clínicas de virilización rápidamente progresiva, sien do el hiperandrogenismo puro el patrón bioquímico hallado. En ambos casos, a pesar de haberse realizado la resección completa, el Ki67 elevado como principal factor pronóstico condujo a categorizarlas como de "alto riesgo de recurrencia". Asimismo, se ha asociado a la gestación y al patrón secretor de glucocorticoides como factores adicionales de mayor riesgo de recurrencia. Este es particularmente elevado dentro de los dos primeros años posteriores al diagnóstico. Existe aún controversia sobre el uso de mitotane adyuvante en estos pacientes, y su inicio está recomendado hasta los tres meses del postquirúrgico. Sin embargo, la evidencia disponible no permite suponer la falta de eficacia si se utiliza fuera de ese período. Los limitantes, como fueron el curso de la gestación y el puerperio inmediato, así como la difi cultad para el acceso a la medicación en nuestro medio, impidieron el inicio precoz del tratamiento adyuvante en ambos casos, aunque surge la inquietud de si aún sería oportuna su instauración.

Abstract Adrenocortical carcinomas are rare tumors, usually hyperfunctioning, with poor overall survival. Frequent age of presentation is described in adults between 40 and 60 years of age, predominantly female. Two unusual cases of adrenal carcinoma diagnosed in young women are presented. The first one was discovered in the sec ond trimester of gestation, with signs and symptoms of hypercortisolism and localized adrenal lesion, which was resolved with complete resection by week 20 of pregnancy. In the second case, the patient begined with clinical manifestations of rapidly progressive virilization, the biochemical pattern being pure hyperandrogenism. In both cases, despite complete resection, the high Ki67 as the main prognostic factor leaded to categorization as "high risk of recurrence". In addition, pregnancy and glucocorticoid secretory pattern have been associated as additional risk factors of recurrence. This is particularly high within the first two years after diagnosis. There is controversy about the use of adjuvant mitotane in these patients, and the general recommendation is to be started no longer than 3 months after surgery. However, the available evidence does not suggest that its use is ineffective beyond that period. Limitations, such as the course of pregnancy and the immediate puerperium, as well as the difficulty of accessing this medication in our environment, prevented the early initiation of adjuvant treatment with mitotane in both cases, although there is still concern whether its administration would still be appropriate.

Ouabain Reverts CUS-Induced Disruption of the HPA Axis and Avoids Long-Term Spatial Memory Deficits.

Ouabain (OUA) is a cardiotonic steroid that modulates Na+, K+ -ATPase activity. OUA has been identified as an endogenous substance that is present in human plasma, and it has been shown to be associated with the response to acute stress in both animals and humans. Chronic stress is a major aggravating factor in psychiatric disorders, including depression and anxiety. The present work investigates the effects of the intermittent administration of OUA (1.8 µg/kg) during the chronic unpredictable stress (CUS) protocol in a rat's central nervous system (CNS). The results suggest that the intermittent OUA treatment reversed CUS-induced HPA axis hyperactivity through a reduction in (i) glucocorticoids levels, (ii) CRH-CRHR1 expression, and by decreasing neuroinflammation with a reduction in iNOS activity, without interfering with the expression of antioxidant enzymes. These changes in both the hypothalamus and hippocampus may reflect in the rapid extinction of aversive memory. The present data demonstrate the ability of OUA to modulate the HPA axis, as well as to revert CUS-induced long-term spatial memory deficits.

Erupción variceliforme de Kaposi en un paciente adulto mayor con desnutrición severa

El eccema herpético o erupción variceliforme de Kaposi, a pesar de ser una infección viral poco frecuente, se considera una urgencia dermatológica, debido a su alto potencial de diseminación, gravedad y mortalidad en adultos. Los pacientes inmunosuprimidos suponen un alto riesgo de colonización cutánea y visceral, además de una mayor probabilidad de presentar otras infecciones causadas por el virus del herpes simple. Se caracteriza por presentar lesiones en piel de tipo vesículas umbilicadas agrupadas o diseminadas que evolucionan a erosiones hemorrágicas perforadas, que se localizan principalmente en cabeza, cuello y tórax; son dolorosas y tardan entre 2-6 semanas en sanar y por lo general se asocian a compromiso sistémico. El diagnóstico es clínico; en caso de duda, se puede utilizar Test de Tzanck, que es una herramienta rápida para confirmar infección por herpes virus; tiene una sensibilidad de 40-80 % y especificidad hasta del 100 %. El manejo consiste en terapia antiviral sistémica; el tratamiento antibiótico está indicado si existe riesgo sobreinfección bacteriana. El tratamiento oportuno de la infección es clave para la evolución hacia la recuperación del paciente.

Eczema herpeticum or Kaposi's varicelliform eruption is a rare viral infection; however, it's considered a dermatologic urgency due to the high potential for dissemination, severity and mortality. Immunosuppressed patients have a high risk of skin and visceral colonization in addition to a higher probability of other infections caused by herpes simplex virus. Kaposi's varicelliform eruption is characterized by vesicopustules, some umbilicated, others eroded and extended in clusters. They may also present hemorrhagic crusts with an erythematous base. The most commonly affected sites are head, neck, and trunk. These lesions are painful, they take from 2 to 6 weeks to heal and are usually associated with systemic signs of infection. Diagnosis is mainly clinical. In case of doubt, the Tzanck allows a rapid diagnostic approach with a sensitivity of 40-80 % and specificity up to 100 % in herpes virus. Antiviral treatment proved to be effective; additional antibiotic treatment is required if there is risk of bacterial infection. The timely treatment of the infection is the key in the evolution towards the recovery of the patient.

Preparation of a novel EGFR specific immunotoxin and its efficacy of anti-colorectal cancer in vitro and in vivo.

OBJECTIVES: Epithelial growth factor receptor (EGFR), as a malignancy marker, is overly expressed in multiple solid tumors including colorectal neoplasms, one of the most prevalent malignancies worldwide. The main objective of this study is to enhance the efficacy of anti-tumor therapy targeting EGFR by constructing a novel EGFR-specific immunotoxin (C-CUS245C) based on Cetuximab and recombinant Cucurmosin (CUS245C). METHODS: E. coli BL21 (DE3) PlysS (E. coli) was used to express CUS245C with a cysteine residue inserting to the C-terminus of Cucurmosin. Then immobilized metal ion affinity chromatography (IMAC) was used to purify CUS245C. The chemical conjugation method was used for the preparation of C-CUS245C. Then dialysis and IMAC were used to purify C-CUS245C. Western blot as well as SDS-PAGE was carried out to characterize the formation of C-CUS245C. At last the anti-colorectal cancer activity of C-CUS245C was investigated in vitro and in vivo. RESULTS: CUS245C with high purity could be obtained from the prokaryotic system. C-CUS245C was successfully constructed and highly purified. The cytotoxicity assays in vitro showed a significant proliferation inhibition of C-CUS245C on EGFR-positive cells for 120 h with IC50 values less than 0.1 pM. Besides, the anti-tumor efficacy of C-CUS245C was remarkably more potent than that of Cetuximab, CUS245C, and C + CUS245C (P < 0.001). Whereas the cytotoxicity of C-CUS245C could hardly be detected on EGFR-null cell line. Our results also showed that C-CUS245C had efficacy of anti-colorectal cancer in mouse xenograft model, indicating the therapeutic potential of C-CUS245C for the targeted therapy of colorectal neoplasms. CONCLUSIONS: C-CUS245C exhibits potent and EGFR-specific cytotoxicity. Insertional mutagenesis technique is worthy to be adopted in the preparation of immunotoxin. Immunotoxin can be highly purified through dialysis followed by IMAC.

The role of allopregnanolone in depressive-like behaviors: Focus on neurotrophic proteins.

Allopregnanolone (3α,5α-tetrahydroprogesterone; pharmaceutical formulation: brexanolone) is a neurosteroid that has recently been approved for the treatment of postpartum depression, promising to fill part of a long-lasting gap in the effectiveness of pharmacotherapies for depressive disorders. In this review, we explore the experimental research that characterized the antidepressant-like effects of allopregnanolone, with a particular focus on the neurotrophic adaptations induced by this neurosteroid in preclinical studies. We demonstrate that there is a consistent decrease in allopregnanolone levels in limbic brain areas in rodents submitted to stress-induced models of depression, such as social isolation and chronic unpredictable stress. Further, both the drug-induced upregulation of allopregnanolone or its direct administration reduce depressive-like behaviors in models such as the forced swim test. The main drugs of interest that upregulate allopregnanolone levels are selective serotonin reuptake inhibitors (SSRIs), which present the neurosteroidogenic property even in lower, non-SSRI doses. Finally, we explore how these antidepressant-like behaviors are related to neurogenesis, particularly in the hippocampus. The protagonist in this mechanism is likely the brain-derived neurotrophic factor (BFNF), which is decreased in animal models of depression and may be restored by the normalization of allopregnanolone levels. The role of an interaction between GABA and the neurotrophic mechanisms needs to be further investigated.

Acute agmatine administration, similar to ketamine, reverses depressive-like behavior induced by chronic unpredictable stress in mice.

Agmatine is an endogenous neuromodulator that has been shown to have antidepressant-like properties. We have previously demonstrated that it can induce a rapid increase in BDNF levels after acute administration, suggesting that agmatine may be a fast-acting antidepressant. To investigate this hypothesis, the present study evaluated the effects of a single administration of agmatine in mice subjected to chronic unpredictable stress (CUS), a model of depression responsive only to chronic treatment with conventional antidepressants. The ability of agmatine to reverse CUS-induced behavioral and biochemical alterations was evaluated and compared with those elicited by the fast-acting antidepressant (ketamine) and the conventional antidepressant (fluoxetine). After exposed to CUS for 14days, mice received a single oral dose of agmatine (0.1mg/kg), ketamine (1mg/kg) or fluoxetine (10mg/kg), and were submitted to behavioral evaluation after 24h. The exposure to CUS caused an increased immobility time in the tail suspension test (TST) but did not change anhedonic-related parameters in the splash test. Our findings provided evidence that, similarly to ketamine, agmatine is able to reverse CUS-induced depressive-like behavior in the TST. Western blot analyses of prefrontal cortex (PFC) demonstrated that mice exposed to CUS and/or treated with agmatine, fluoxetine or ketamine did not present alterations in the immunocontent of synaptic proteins [i.e. GluA1, postsynaptic density protein 95 (PSD-95) and synapsin]. Altogether, our findings indicate that a single administration of agmatine is able to reverse behavioral alterations induced by CUS in the TST, suggesting that this compound may have fast-acting antidepressant-like properties. However, there was no alteration in the levels of synaptic proteins in the PFC, a result that need to be further investigated in other time points.

[Biomineralization of copper in Candida fukuyamaensis RCL-3]. / Biomineralización de cobre en Candida fukuyamaensis RCL-3.

Candida fukuyamaensis RCL-3 yeast has the ability to decrease copper concentration in a culture medium. High copper concentrations change the cell color from white/cream to brown. The effect of color change ceases with the addition of KCN or when cells are grown in a culture medium without sulfate ions. These results could be associated with CuS bioaccumulation in the cell surface. This report revealed that mineralization would be a mechanism used by this yeast for copper bioremediation.

Incorporação de ômega-3 no tecido muscular da tilápia do Nilo alimentada com dietas contendo silagem de cabeça de camarão

The aim of the present study was to assess the effect of diets supplemented with shrimp-head silage on the incorporation of n-3 in the Nile tilapia fillets. EPA (eicosapentaenoic), DHA (docosahexanoic) and total lipids were determined by chromatography in feeds and the muscles of tilapia specimens were submitted to diets supplemented with a varying degree of silage (0, 4, 8, 12, 16%). The total lipid content ranged from 6.70 to 10.30% in the diets and from 0.79 to 1.37% in the tilapia fillets. In diet with high concentration of silage, it was observed an increase in the level of omega-3 (r=0.92). There was an incorporation of 0.59% (1.34mg 100g-1) and 9.5% (40.50mg 100g-1) of EPA and DHA, respectively, when the addition of silage to the diet was 16%. The results implication states that the inclusion of 16% of the shrimp head silage in the diet for tilapias increases the levels of incorporation of EPA and DHA in the filet.

O objetivo deste estudo foi avaliar a incorporação de ômega-3 EPA (eicosapentaenóico) e DHA (docosahexanóico) no tecido muscular de tilápias do Nilo alimentadas com dietas suplementadas com diferentes níveis de silagem de cabeça de camarão Litopenaeus vannamei (0, 4, 8, 12, 16%). Foram analisados os teores de EPA, DHA e lipídios totais das dietas e dos tecidos musculares das tilápias. Os teores de lipídios totais variaram entre 6,70 a 10,30% nas dietas e 0,79 a 1,37% no tecido muscular. Através da análise de regressão, houve um aumento nos teores de ômega-3 (r=0,92) com a inclusão da silagem na dieta. Foi observada incorporação de 0,59% (1,34mg 100g-1) e 9,5% (40,50mg 100g-1) de EPA e DHA, respectivamente, no tratamento com 16% de silagem na dieta. A inclusão de 16% da silagem ácida de cabeça de camarão na dieta das tilápias aumentou significativamente os níveis de incorporação de EPA e DHA no filé.

Incorporação de ômega-3 no tecido muscular da tilápia do Nilo alimentada com dietas contendo silagem de cabeça de camarão

The aim of the present study was to assess the effect of diets supplemented with shrimp-head silage on the incorporation of n-3 in the Nile tilapia fillets. EPA (eicosapentaenoic), DHA (docosahexanoic) and total lipids were determined by chromatography in feeds and the muscles of tilapia specimens were submitted to diets supplemented with a varying degree of silage (0, 4, 8, 12, 16%). The total lipid content ranged from 6.70 to 10.30% in the diets and from 0.79 to 1.37% in the tilapia fillets. In diet with high concentration of silage, it was observed an increase in the level of omega-3 (r=0.92). There was an incorporation of 0.59% (1.34mg 100g-1) and 9.5% (40.50mg 100g-1) of EPA and DHA, respectively, when the addition of silage to the diet was 16%. The results implication states that the inclusion of 16% of the shrimp head silage in the diet for tilapias increases the levels of incorporation of EPA and DHA in the filet.

O objetivo deste estudo foi avaliar a incorporação de ômega-3 EPA (eicosapentaenóico) e DHA (docosahexanóico) no tecido muscular de tilápias do Nilo alimentadas com dietas suplementadas com diferentes níveis de silagem de cabeça de camarão Litopenaeus vannamei (0, 4, 8, 12, 16%). Foram analisados os teores de EPA, DHA e lipídios totais das dietas e dos tecidos musculares das tilápias. Os teores de lipídios totais variaram entre 6,70 a 10,30% nas dietas e 0,79 a 1,37% no tecido muscular. Através da análise de regressão, houve um aumento nos teores de ômega-3 (r=0,92) com a inclusão da silagem na dieta. Foi observada incorporação de 0,59% (1,34mg 100g-1) e 9,5% (40,50mg 100g-1) de EPA e DHA, respectivamente, no tratamento com 16% de silagem na dieta. A inclusão de 16% da silagem ácida de cabeça de camarão na dieta das tilápias aumentou significativamente os níveis de incorporação de EPA e DHA no filé.

Incorporação de ômega-3 no tecido muscular da tilápia do Nilo alimentada com dietas contendo silagem de cabeça de camarão

The aim of the present study was to assess the effect of diets supplemented with shrimp-head silage on the incorporation of n-3 in the Nile tilapia fillets. EPA (eicosapentaenoic), DHA (docosahexanoic) and total lipids were determined by chromatography in feeds and the muscles of tilapia specimens were submitted to diets supplemented with a varying degree of silage (0, 4, 8, 12, 16%). The total lipid content ranged from 6.70 to 10.30% in the diets and from 0.79 to 1.37% in the tilapia fillets. In diet with high concentration of silage, it was observed an increase in the level of omega-3 (r=0.92). There was an incorporation of 0.59% (1.34mg 100g-1) and 9.5% (40.50mg 100g-1) of EPA and DHA, respectively, when the addition of silage to the diet was 16%. The results implication states that the inclusion of 16% of the shrimp head silage in the diet for tilapias increases the levels of incorporation of EPA and DHA in the filet.

O objetivo deste estudo foi avaliar a incorporação de ômega-3 EPA (eicosapentaenóico) e DHA (docosahexanóico) no tecido muscular de tilápias do Nilo alimentadas com dietas suplementadas com diferentes níveis de silagem de cabeça de camarão Litopenaeus vannamei (0, 4, 8, 12, 16%). Foram analisados os teores de EPA, DHA e lipídios totais das dietas e dos tecidos musculares das tilápias. Os teores de lipídios totais variaram entre 6,70 a 10,30% nas dietas e 0,79 a 1,37% no tecido muscular. Através da análise de regressão, houve um aumento nos teores de ômega-3 (r=0,92) com a inclusão da silagem na dieta. Foi observada incorporação de 0,59% (1,34mg 100g-1) e 9,5% (40,50mg 100g-1) de EPA e DHA, respectivamente, no tratamento com 16% de silagem na dieta. A inclusão de 16% da silagem ácida de cabeça de camarão na dieta das tilápias aumentou significativamente os níveis de incorporação de EPA e DHA no filé.

Utilização de levedura íntegra e seus derivados em dietas para juvenis de tilápia do Nilo

This study has evaluated the effect of adding dried yeast and its by-products in diets for juveniles of Nile tilapia. It was used 144 juveniles of male tilapia (average weight of 52.1g) distributed in 12 fiberglass tanks (250L), in completely randomized design, composed by 4 treatments and 3 replicates. The fish were fed ad libitum, 2 times per the day during 60 days, with isoproteic (28% CP) and isocaloric diets (2.900kcal ED kg-1) with dried yeast of sugar cane (LI), disrupted yeast cells (LA) and yeast cell wall (PC) added in the ratio of 25% of the total crude protein, compared with a control diet (CO), without yeast addition. No significant differences were observed for feed conversion and proteic efficiency rate. However, the weight gain was better in the fish fed with diets LA (114.70g) and PC (131.03g), as well as in relation to the specific growth rate (LA=1.79 and PC=1.93%), crude protein in the weight gain (LA=14.45 and PC=15.62%) and body protein content (LA=14.89 and PC=15.67g 100g-1). The by-products, cellular wall and disrupted yeast cell of sugar can be used in diets for tilapia juveniles.

Neste trabalho, avaliou-se a adição de células íntegras de levedura e seus derivados em dietas para juvenis de tilápia do Nilo. Foram utilizados 144 juvenis machos de tilápia (peso médio de 52,1g) distribuídos em 12 tanques de fibra de vidro (250L), em delineamento inteiramente casualizado, composto por quatro tratamentos e três repetições. Os peixes foram alimentados ad libitum, duas vezes ao dia durante 60 dias, com dietas isoproteicas (28% PB) e isocalóricas (2.900kcal de ED kg-1) contendo levedura íntegra de cana-de-açúcar (LI), levedura autolisada (LA) e parede celular (PC) adicionados na proporção de 25% da proteína bruta total, comparadas com uma dieta controle (CO), sem adição de levedura. Não foram observadas diferenças significativas para conversão alimentar aparente e taxa de eficiência protéica. No entanto, o ganho em peso foi melhor nos peixes alimentados com as dietas LA (114,70g) e PC (131,03g), assim como em relação à taxa de crescimento específico (LA=1,79 e PC=1,93%), à proteína bruta no ganho de peso (LA=14,45 e PC=15,62%) e ao conteúdo corporal proteico (LA=14,89 e PC=15,67g 100g-1). As frações, a parede celular e a levedura autolisada de cana-de-açúcar podem ser utilizadas em dietas para juvenis de tilápia.

Utilização de levedura íntegra e seus derivados em dietas para juvenis de tilápia do Nilo

This study has evaluated the effect of adding dried yeast and its by-products in diets for juveniles of Nile tilapia. It was used 144 juveniles of male tilapia (average weight of 52.1g) distributed in 12 fiberglass tanks (250L), in completely randomized design, composed by 4 treatments and 3 replicates. The fish were fed ad libitum, 2 times per the day during 60 days, with isoproteic (28% CP) and isocaloric diets (2.900kcal ED kg-1) with dried yeast of sugar cane (LI), disrupted yeast cells (LA) and yeast cell wall (PC) added in the ratio of 25% of the total crude protein, compared with a control diet (CO), without yeast addition. No significant differences were observed for feed conversion and proteic efficiency rate. However, the weight gain was better in the fish fed with diets LA (114.70g) and PC (131.03g), as well as in relation to the specific growth rate (LA=1.79 and PC=1.93%), crude protein in the weight gain (LA=14.45 and PC=15.62%) and body protein content (LA=14.89 and PC=15.67g 100g-1). The by-products, cellular wall and disrupted yeast cell of sugar can be used in diets for tilapia juveniles.

Neste trabalho, avaliou-se a adição de células íntegras de levedura e seus derivados em dietas para juvenis de tilápia do Nilo. Foram utilizados 144 juvenis machos de tilápia (peso médio de 52,1g) distribuídos em 12 tanques de fibra de vidro (250L), em delineamento inteiramente casualizado, composto por quatro tratamentos e três repetições. Os peixes foram alimentados ad libitum, duas vezes ao dia durante 60 dias, com dietas isoproteicas (28% PB) e isocalóricas (2.900kcal de ED kg-1) contendo levedura íntegra de cana-de-açúcar (LI), levedura autolisada (LA) e parede celular (PC) adicionados na proporção de 25% da proteína bruta total, comparadas com uma dieta controle (CO), sem adição de levedura. Não foram observadas diferenças significativas para conversão alimentar aparente e taxa de eficiência protéica. No entanto, o ganho em peso foi melhor nos peixes alimentados com as dietas LA (114,70g) e PC (131,03g), assim como em relação à taxa de crescimento específico (LA=1,79 e PC=1,93%), à proteína bruta no ganho de peso (LA=14,45 e PC=15,62%) e ao conteúdo corporal proteico (LA=14,89 e PC=15,67g 100g-1). As frações, a parede celular e a levedura autolisada de cana-de-açúcar podem ser utilizadas em dietas para juvenis de tilápia.