ABSTRACT
The excessive application of phosphorus (P) fertiliser and its poor utilisation efficiency have led to significant amounts of P being retained in agricultural soils in unavailable forms. The application of alkaline lignin to soil and its inoculation with arbuscular mycorrhizal fungi (AMF) have both been shown to improve plant P nutrition. However, their combined effects on soil P transformation remain unclear, particularly in cadmium (Cd)-contaminated soils. A potting experiment was conducted to examine the combined effects of AMF and alkaline lignin on soil P and Cd bioavailability and on the uptake of P and Cd by lettuce (Lactuca sativa L.) that were grown for 56 d in a growth chamber. Combined AMF and alkaline lignin treatment increased soil P availability and alkaline phosphatase activity. It furthermore increased bioavailable Cd concentrations of rhizosphere and bulk soils by 48 % and 72 %, respectively, and the Cd concentration in roots by 85 %, but the Cd concentration was not affected in the edible parts (shoots) of the lettuce. Moreover, the combined treatment increased shoot biomass by 26-70 % and root biomass by 99-164 %. Our findings suggested that the combined use of AMF and alkaline lignin mobilised both P and Cd in soil but did not increase the accumulation of Cd in the shoots of plants growing in Cd-contaminated soils, these results would provide guideline for increasing Cd tolerance of plants and their yield.
Subject(s)
Cadmium , Lactuca , Lignin , Mycorrhizae , Phosphorus , Soil Pollutants , Mycorrhizae/physiology , Lactuca/metabolism , Cadmium/metabolism , Phosphorus/metabolism , Soil Pollutants/metabolism , Lignin/metabolism , Soil/chemistry , Soil Microbiology , FertilizersABSTRACT
Edible offal of farmed animals can accumulate cadmium (Cd). However, no studies have investigated Cd bioavailability and its health effects. Here, based on mouse models, market pork kidney samples exhibited high Cd relative bioavailability of 74.5 ± 11.2% (n = 26), close to 83.8 ± 7.80% in Cd-rice (n = 5). This was mainly due to high vitamin D3 content in pork kidney, causing 1.7-2.3-fold up-regulated expression of duodenal Ca transporter genes in mice fed pork kidney compared to mice fed Cd-rice, favoring Cd intestinal absorption via Ca transporters. However, although pork kidney was high in Cd bioavailability, subchronic low-dose (5% in diet) consumption of two pork kidney samples having 0.48 and 0.97 µg Cd g-1 dw over 35 d did not lead to significant Cd accumulation in the tissue of mice fed Cd-free rice but instead remarkably decreased Cd accumulation in the tissue of mice fed Cd-rice (0.48 µg Cd g-1) by â¼50% and increased abundance of gut probiotics (Faecalibaculum and Lactobacillus). Overall, this study contributed to our understanding of the bioavailability and health effects associated with Cd in edible offal, providing mechanistic insights into pork kidney consumption safety based on Cd bioavailability.
Subject(s)
Cadmium , Kidney , Animals , Cadmium/metabolism , Mice , Kidney/metabolism , Swine , Biological AvailabilityABSTRACT
Cadmium (Cd) is recognized as a significant hazard to human health, with exposure linked to a variety of adverse outcomes including various cancers, cardiovascular diseases, chronic kidney disease, and osteoporosis. Residing in areas contaminated with Cd is undoubtedly a risk factor for developing the aforementioned conditions. However, the risk of developing Cd-related disorders is not uniform among individuals. Deficiencies in iron, zinc, and calcium, along with iron deficiency anemia, decreased lung function often seen in chronic obstructive pulmonary disease (COPD), and low circulating levels of parathyroid hormone (PTH), may enhance Cd intestinal absorption. Conversely, chronic liver disorders can lead to the progressive loss of hepatocytes and the release of free Cd into the circulation, resulting in elevated Cd blood levels. Moreover, studies comparing Cd blood levels between different regions within a country or between two groups of individuals, for example, those with and without osteoporosis, should consider all variables that may impact Cd levels. These include age, sex, alcohol consumption, blood levels of iron, calcium, and zinc, the presence of anemia, COPD, PTH levels, and the presence of liver or kidney disease. In this review, we delve into all factors that could influence Cd blood levels, providing a comprehensive analysis.
Subject(s)
Cadmium , Humans , Cadmium/blood , Biological AvailabilityABSTRACT
Both selenium (Se) and gibberellins (GA3) can alleviate cadmium (Cd) toxicity in plants. However, the application of Se and GA3 as foliar spray to against Cd stress on soybean and its related mechanisms have been poorly explored. Herein, this experiment evaluated the effects of Se and GA3 alone and combined application on soybean rhizosphere microenvironment, Cd accumulation and growth of soybean seedlings. The results revealed that both Se and GA3 can effectively decrease the accumulation of Cd in soybean seedlings. Foliar application of Se, GA3 and their combination reduced Cd contents in soybean seedlings respectively by 21.70 %, 27.53 % and 45.07 % when compared with the control treatment, suggest a synergistic effect of Se and GA3 in decreasing Cd accumulation. Se and GA3 also significantly increased diversity and abundance of the metabolites in rhizosphere, which consequently played an important role in shaping rhizosphere bacteria community and improve rhizosphere soil physicochemical properties of Cd contaminated soil, as well as decreased the Cd available forms contents but enhance the immobilized form levels. Overall, this study affords a novel approach on mitigating Cd accumulation in soybean seedlings which is attributed to Se and GA3 regulated interplay among rhizosphere soil metabolites, bacteria community and cadmium speciation.
Subject(s)
Cadmium , Gibberellins , Glycine max , Rhizosphere , Selenium , Soil Microbiology , Soil Pollutants , Glycine max/drug effects , Glycine max/metabolism , Glycine max/growth & development , Cadmium/toxicity , Cadmium/metabolism , Gibberellins/metabolism , Gibberellins/pharmacology , Soil Pollutants/metabolism , Soil Pollutants/toxicity , Selenium/metabolism , Bacteria/metabolism , Bacteria/drug effects , Bacteria/classification , Seedlings/drug effects , Seedlings/metabolism , Seedlings/growth & developmentABSTRACT
Microbial metabolic activities in rhizosphere soil play a critical role in plant nutrient utilization and metal availability. However, its specific characteristics and influence on endophyte assisted phytoremediation remains unclear. In this study, an endophyte strain Bacillus paramycoides (B. paramycoides) was inoculated in the rhizosphere of Phytolacca acinosa (P. acinosa), and microbial metabolic characteristics of rhizosphere soils were analyzed using Biolog system to investigate how they influence phytoremediation performance of different types of cadmium contaminated soil. The results indicated that endophyte B. paramycoides inoculation enhanced bioavailable Cd percentage by 9-32%, resulting in the increased Cd uptake (32-40%) by P. acinosa. With endophyte inoculation, the utilization of carbon sources was significantly promoted by 4-43% and the microbial metabolic functional diversity increased by 0.4-36.8%. Especially, B. paramycoides enhanced the utilization of recalcitrant substrates carboxyl acids, phenolic compounds and polymers by 48.3-225.6%, 42.4-65.8% and 15.6-25.1%, respectively. Further, the microbial metabolic activities were significant correlated with rhizosphere soil microecology properties and impact phytoremediation performance. This study provided new insight into the microbial processes during endophyte assisted phytoremediation.
Subject(s)
Cadmium , Soil Pollutants , Cadmium/analysis , Biodegradation, Environmental , Rhizosphere , Endophytes/metabolism , Soil Pollutants/analysis , Plant Roots/metabolism , Soil/chemistryABSTRACT
The widespread heavy metal contamination of agricultural soils poses an enormous challenge to food safety. To evaluate the Cd immobilization potential of vermicompost combined with modified shell powder (VMSP) on Cd-contaminated soil, batch adsorption tests and field experiments were conducted. First, the Cd2+ removal characteristics and adsorption mechanisms of vermicompost (V), vermicompost combined with shell powder (VSP), and VMSP in an aqueous solution were investigated by batch tests. Then, 3 kg·m2 V, VSP, and VMSP doses were applied to Cd-contaminated farmland soils as soil amendments to plant green garlic (Allium sativum L.) and investigate their Cd immobilization effects in Cd-contaminated soils. Batch adsorption tests showed that VMSP was most effective for Cd2+ removal, with adsorption rates as high as 85.7-99.79% and desorption rates of approximately 1.25-1.34%. Combining further characterization analysis of VMSP, it was demonstrated that the adsorption mechanism of Cd2+ was monolayer chemisorption, mainly involving the complexation reaction of Cd2+ with organic functional groups and the precipitation reaction of Cd2+ with mineral elements. The field experiment showed that adding V, VSP, and VMSP effectively inhibited the enrichment of Cd in green garlic, and the Cd content was reduced by 42.18%, 46.88%, and 68.75%, respectively. However, only the Cd content of green garlic treated with VMSP was lower than the national standard for food safety in China (Cd≤ 0.2 mg·kg-1). V, VSP, and VMSP additions improved soil fertility and reduced Cd bioavailability in the soil by 15.5%, 18.9%, and 36.3%, respectively. In addition, V, VSP, and VMSP addition increased bacterial diversity and improved bacterial communities and functions in the soil by improving basic soil properties and reducing Cd-related toxicity. The results indicated that VMSP is a promising amendment for Cd immobilization in Cd-contaminated farmland soils.
ABSTRACT
Both soil irrigation and sulfur (S) are associated with the precipitation of cadmium (Cd)-sulfide in paddy soil, their interaction affecting on Cd solubility and extractability is still unknown. This study primarily discusses the effect of exogenous S addition on the bioavailability of Cd in paddy soil under unsteady pe + pH conditions. The experiment was treated with three different water strategies: continuous dryness (CD), continuous flooding (CF), and alternating dry-wet cycles for one cycle (DW). These strategies were combined with three different S concentrations. The results indicate that the CF treatment, particularly when combined with S addition, had the most significant effect on reducing pe + pH and Cd bioavailability in the soil. The reduction of pe + pH from 10.2 to 5.5 resulted in a decrease in soil Cd availability by 58.3%, and Cd accumulation in rice grain by 52.8%, compared to the other treatments. While it was more conducive to the formation of iron plaque on the root surface in DW treatment with S addition at rice maturing stage and enhanced the gathering of Fe/S/Cd. Structural equation model (SEM) analysis further confirmed a significant negative correlation (r = -0.916) between the abundance of soil Fer-reducing bacteria (FeRB) and sulfate-reducing bacteria (SRB) like Desulfuromonas, Pseudomonas, Geobacter, and the Cd content in rice grains. This study provides a basic mechanistic understanding of how soil redox status (pe + pH), S addition, and FeRB/SRB interacted with Cd transfer in paddy soil-rice tissues.
Subject(s)
Desulfovibrio , Oryza , Soil Pollutants , Soil/chemistry , Oryza/chemistry , Cadmium/analysis , Soil Pollutants/analysis , Iron/analysis , Bacteria , Hydrogen-Ion ConcentrationABSTRACT
This present study investigated pork bone-derived biochar as a promising amendment to reduce Cd accumulation and alleviate Cd-induced oxidative stress in rice. Micro/nanoscale bone char (MNBC) pyrolyzed at 400 °C and 600 °C was synthesized and characterized before use. The application rates for MNBCs were set at 5 and 25 g·kg-1 and the Cd exposure concentration was 15 mg·kg-1. MNBCs increased rice biomass by 15.3-26.0% as compared to the Cd-alone treatment. Both types of MNBCs decreased the bioavailable Cd content by 27.4-54.8%; additionally, the acid-soluble Cd fraction decreased by 10.0-12.3% relative to the Cd alone treatment. MNBC significantly reduced the cell wall Cd content by 50.4-80.2% relative to the Cd-alone treatment. TEM images confirm the toxicity of Cd to rice cells and that MNBCs alleviated Cd-induced damage to the chloroplast ultrastructure. Importantly, the addition of MNBCs decreased the abundance of heavy metal tolerant bacteria, Acidobacteria and Chloroflexi, by 29.6-41.1% in the rhizosphere but had less impact on the endophytic microbial community. Overall, our findings demonstrate the significant potential of MNBC as both a soil amendment for heavy metal-contaminated soil remediation and for crop nutrition in sustainable agriculture.
Subject(s)
Metals, Heavy , Oryza , Soil Pollutants , Cadmium/toxicity , Cadmium/analysis , Oryza/chemistry , Rhizosphere , Metals, Heavy/analysis , Soil/chemistry , Soil Pollutants/toxicity , Soil Pollutants/analysis , Charcoal/chemistryABSTRACT
Combined pollution from microplastics (MPs) and cadmium (Cd) can influence soil environment and soil biota, altering plant growth and development, and Cd mobilization. We investigated the effects of polystyrene (PS) and polypropylene (PP) MPs alongside Cd on soil Cd bioavailability, rhizosphere soil metabolomics, bacterial community structure, and maize (Zea mays L.) growth in two soil types (red soil and cinnamon soil). Although the addition of PS/PP-Cd promoted Cd accumulation in maize plants overall, there were large-particle-size- and small-particle-size-dependent effects in the red soil and cinnamon soil, respectively. The difference is mainly due to the capacity of the large particle size MPs to significantly reduce soil pH, improve soil electrical conductivity (EC), promote active Cd, and intensify Cd mobilization in red soil. In contrast, small-size MPs in cinnamon soil promoted the synthesis and secretion of rhizosphere amino acids and soil metabolites, thus promoting Cd absorption by maize roots. Soil microorganisms also improved Cd bioavailability via C-related functional bacteria. Overall, our study provides novel insights on the potential effects of combined MPs and Cd pollution on soil ecology and agricultural production, enhancing our understanding of rhizosphere metabolites in different soils.
Subject(s)
Cadmium , Soil Pollutants , Cadmium/metabolism , Microplastics/analysis , Zea mays/metabolism , Plastics/analysis , Plant Roots/metabolism , Soil/chemistry , Amino Acids/metabolism , Soil Pollutants/metabolism , RhizosphereABSTRACT
Compared with the widespread and serious heavy metal contamination in soils, microplastic pollution has gained attention only recently. Little is known about how microplastics affect the distribution of heavy metals in soils, especially across soil components level. In this study, a 180-day soil aging experiment and soil density fractionation were performed to investigate the effect of polypropylene (PP) microplastics on the binding behavior of cadmium (Cd) to solid components, i.e. particulate organic matter, organo-mineral complexes (OMC), and mineral. Results showed addition of 2-10% microplastics in soils induced the decomposition of OMC fraction by 10.88-23.10%. Compared to the control, the content of dissolved organic carbon increased, and pH, humic substances, and soil organic matter decreased with microplastics. After 180d of aging, the content of Cd in OMC fraction increased by 17.92%, while microplastics made Cd contents decline by 10.01-19.75%. The impacts strongly depended on the dose and surface characteristic of microplastics. Overall, PP microplastics increased the concentration of bioavailable Cd in soils via decreasing soil retention of Cd by the OMC fraction. These findings based on the solid components level will provide a new perspective for understanding microplastics effects on soil systems and pollutants.
Subject(s)
Metals, Heavy , Soil Pollutants , Soil/chemistry , Cadmium/chemistry , Microplastics , Plastics/metabolism , Polypropylenes , Biological Availability , Soil Pollutants/analysis , Metals, Heavy/analysis , Particulate MatterABSTRACT
The performances of passivation materials mitigating Cadmium (Cd) bioavailability considerably vary with the pH condition of Cd-contaminated soils. However, less information was available for the method of improving Cd passivation efficiency taking into account the pH of the targeted soil. Furthermore, the underlying mechanism of Cd availability mitigation in soils with different pH has not been clearly explored. In this study, cotton straw biochar (CSB) and its modified products using NaOH (CSB-NaOH) were prepared and applied in two kinds of Cd-contaminated soils with different pH. It was found that CSB-NaOH was more effective than CSB in regulating the Cd bioavailability in the acid soil, while the opposite tendency was observed in alkaline soil. The difference of the Cd passivation efficiency is correlated with contributions of various Cd-biochar binding mechanisms, which cation exchange mechanism is largely eliminated for CSB-NaOH. The interaction of Cd with CSB/CSB-NaOH was further evidenced through characterization results of Scan Electron Microscopy (SEM), X-Ray Diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR) and X-ray Photoelectron spectroscopy (XPS). Characterization results proved that carboxyl, hydroxyl and ethyl groups were the key functional groups involved in Cd passivation. XPS results showed that Cd binding methods varied between CSB and CSB-NaOH, which Cd2+ and Cd-O were the main form of Cd binding to CSB while Cd-O was the main form on CSB-NaOH. In this work, it was demonstrated that in acid soil, pH change caused by biochar plays a more significant role in controlling the Cd bioavailability, while in alkaline soil, the strength of the Cd-biochar interaction is more decisive for the Cd passivation efficiency. This work provides information on how to select the suitable passivator to decrease the Cd bioavailability in terms of different soil pH and property.
Subject(s)
Cadmium , Soil Pollutants , Biological Availability , Cadmium/analysis , Charcoal/chemistry , Hydrogen-Ion Concentration , Sodium Hydroxide , Soil/chemistry , Soil Pollutants/analysisABSTRACT
Rice consumption is one of the major cadmium (Cd) exposure routes for human. Bangladeshi people have historically subsisted on a rice-based diet; however, only a few reports have investigated Cd accumulation by different rice cultivars in Bangladesh. This study was designed to investigate the uptake and accumulation of Cd in different rice cultivars and associated health risks to humans eating rice. A pot experiment was conducted to grow eight amon rice varieties under control, 5 and 10 mg Cd/kg soil under open air conditions. After harvesting the Cd fractionation, bioavailable Cd and rice grain Cd content were determined. Cd spiked as Cd2+ enriched the Cd bioavailability to plant by 35% (in 5 mg/kg stress) and 85% (in 10 mg/kg stress). There were variations among the rice varieties in their ability to accumulate Cd in grain and this was found to be 15-fold higher under control conditions. Grain Cd content significantly differed among the rice varieties at each level of soil Cd. In this study, BR-52 emerged as the most Cd-safe cultivar followed by BR-75, Rani salut, BR-71, BR-49, BR-76, BR-87 and lastly, BINA-7. Most of the agronomic parameters of rice concerning yield were affected by both rice varieties and soil Cd level. In different rice varieties, rhizosphere pH increased through root exudation which ultimately produced equilibria among the five major soil Cd fractions so that Cd became bioavailable to plants. All rice varieties showed high hazard quotient (HQ) values under Cd stress conditions and posed a risk to human health. For noncarcinogenic health risk assessment through HQ, we recommend 0.1 mg Cd/kg rice grain be used as the maximum allowable concentration (MAC) in calculating health risk for Bangladeshi people.
Subject(s)
Oryza , Soil Pollutants , Bangladesh , Cadmium/analysis , Edible Grain/chemistry , Humans , Soil , Soil Pollutants/analysisABSTRACT
Biochar remediation efficiency could be enhanced through numerous treatments such as acids treatment. Still, there has little work done on H3PO4-treated biochar particularly biochar derived from Achnatherum splendens L. feedstock. Therefore, the present study has been conducted to further explore the potential effect of A. splendens L.-derived biochar treated with H3PO4 on bioavailability of Cd and wheat growth in Cd contaminated soil. Phosphoric acid and untreated biochar each applied at the rate of 1% and 2% to Cd contaminated/spiked soil in pots and having one contaminated/spiked control without biochars amendment. The results show that 2% phosphoric acid-treated biochar has the most significant increase in plant height, shoot dry weight, and grain yield of wheat as compared to contaminated control. As compared to contaminated control, maximum improvement in total chlorophyll contents, photosynthetic rate, transpiration rate, and stomatal conductance occurred with 2% phosphoric acid-treated biochar. The 2% phosphoric acid-treated biochar also declined bioavailable Cd in soil by 53%, and its accumulation in shoot and grain by 65% and 90%, respectively, compared to contaminated control. Overall, phosphoric acid-treated biochar most effectively immobilized Cd in soil and reducing its uptake and translocation to grains. Therefore, A. splendens L.-derived biochar treated with phosphoric acid could be successfully utilized for remediation of contaminated soil.
Subject(s)
Cadmium , Soil Pollutants , Biological Availability , Cadmium/analysis , Charcoal/pharmacology , Edible Grain/chemistry , Phosphoric Acids , Soil , Soil Pollutants/analysis , TriticumABSTRACT
BACKGROUND: Cd seriously threatens soil environment, remedying Cd in farmland and clearing the response of soil environment to modifiers in Cd-contaminated soils is necessary. In this study, the effects of cotton straw biochar and compound Bacillus biofertilizer used as modifiers on the biochemical properties, enzyme activity, and microbial diversity in Cd-contaminated soils (1, 2, and 4 mg·kg-1) were investigated. RESULTS: The results showed that both cotton straw biochar and compound Bacillus biofertilizer could improve the soil chemical characteristics, including the increase of soil C/N ratio, electrical conductance (EC) and pH, and the most important decrease of soil available Cd content by 60.24% and 74.34%, respectively (P < 0.05). On the other hand, adding cotton straw biochar and compound Bacillus biofertilizer in Cd stressed soil also improved soil biological characteristics. Among them, cotton straw biochar mainly through increasing soil alkaline phosphatase activity and improve bacteria abundance, compound Bacillus biofertilizer by increasing soil invertase, alkaline phosphatase, catalase, and urease activity increased bacterial community diversity. On the whole, the decrease of soil available Cd was mainly caused by the increase of soil pH, C/N, urease and alkaline phosphatase activities, and the relative abundance of Acidobacteria and Proteobacteria. CONCLUSIONS: In summary, the applications of cotton straw biochar and compound Bacillus biofertilizer could decrease soil available Cd concentration, increase soil bacterial community diversity and functions metabolism, and reduce the damage of Cd stress, compared with cotton straw biochar, compound Bacillus biofertilizer was more effective in immobilizing Cd and improving soil environmental quality.
Subject(s)
Bacillus/metabolism , Cadmium/analysis , Charcoal/pharmacology , Fertilizers/microbiology , Soil Microbiology , Soil Pollutants/analysis , Soil/chemistry , Biological Availability , Cadmium/metabolism , Farms , Fertilizers/analysis , Oryza/drug effects , Oryza/microbiologyABSTRACT
Sulfur (S) can decrease the bioavailability of Cd in paddy soil, and therefore reduce Cd uptake by rice. However, the dynamic influence mechanism of different forms of sulfur on the bioavailability of cadmium in soil has not been systematically studied. In this study, we used Diffusive gradients in thin films (DGT) technology and soil pore water sampling technology to investigate the effects of different types of S application on the bioavailability of Cd. The three forms of S are elemental sulfur (S0), sodium sulfate (SO42-), and mercapto-grafted palygorskite (MP), which have been treated in six ways according to CK, SL, SH, SO42L, SO42H, MP (L and H are the low and high levels of treatments). The results showed that soluble and labile Cd concentration was quickly fixed after flooding but activated after rice transplantation. Both MP and SL treatments increased the content of dissolved organic carbon (DOC) and significantly reduced the accumulation of Cd in roots and grains. The Cd content of roots treated with MP was only 0.50 mg kg-1, which was 77.6% lower than the Cd content in CK (2.22 mg kg-1). S promotes the reduction and dissolution of Fe, and the formation of FeS/FeS2 is also one of the reasons for the low bioavailability of Cd, while the SH treatment has a greater effect on lowering pH, so the effect of fixing Cd is not obvious. In addition, SO42- treatment delays the soil reduction process, so it cannot effectively fix Cd. At the stage of rice maturity, dissolved Cd increased in S treatment, but MP treatment did not have this phenomenon. The possible reason was the consumption of DOC or the oxidation of CdS which caused Cd to enter the soil solution. Taken together, Fe-S play a critical role in controlling the mobilization of Cd in paddy systems.
Subject(s)
Oryza , Soil Pollutants , Cadmium/analysis , Soil , Soil Pollutants/analysis , SulfurABSTRACT
Plant growth and heavy metal (HM) accumulation is affected by heavy metal bioavailability and nutrient content in soil aggregates during endophyte-assisted phytoremediation. In this study, we evaluated the influence of endophytes inoculation on P. acinosa HM accumulation and soil aggregate physicochemical properties and explored the correlation among them. Endophyte inoculation increased the plant growth and Cd accumulation by 7.95-25.13% and 3.27-19.22%, respectively and the soil aggregate was redistributed with a decrease of 1.88-5.41% of the clay fraction. The available nitrogen, phosphorus and potassium, and organic matter in macro-aggregate and micro-aggregate were significantly improved with endophyte inoculation. In addition, compared to the no inoculation group, endophytes inoculation enhanced the bioavailability of Cd in macro-aggregates by 4.92-15.00% and in micro-aggregate by 0-9.37%. Both multiple linear regression analysis and the structural equation modeling (SEM) analysis showed that the Cd accumulation in P. acinosa was mainly depended on the Cd bioavailability in macro-aggregates and micro-aggregates. In general, this study helped to improve our understanding of soil aggregate HM bioavailability and nutrient content distribution characteristics under endophyte inoculation, which could further explain the mechanisms of endophytes in plant growth promoting and HM accumulation improving.
Subject(s)
Phytolacca , Soil Pollutants , Biodegradation, Environmental , Cadmium , Endophytes , Nutrients , Soil , Soil Pollutants/analysisABSTRACT
The bioavailability of heavy metal and growth of hyperaccumulator are key factors controlling the phytoextraction of heavy metal from soil. In this study, the efficacy and potential microbial mechanisms of digestate application in enhancing Cd extraction from soil by Pennisetum hybridum were investigated. The results showed that digestate application significantly promoted the height, tiller number, and biomass yield of Pennisetum hybridum. The application also increased the activities of urease, sucrase, dehydrogenase, available Cd contents of rhizosphere soils (from 2.21 to 2.46 mg kg-1), and the transfer factors of Cd from root to shoot and leaf. Assuming three annual harvests, digestate application would substantially reduce time needed for Pennisetum hybridum to completely absorb Cd from soil-from 15-16 yr-10 yr. Furthermore, the results of microbial community diversity analysis showed that digestate irrigation was more facilitated for the growth of the predominant bacteria, which were Actinobacteria and Chloroflexi at phylum level, and Sphingomonas and Nitrospiraat genus level, which mainly have the functions of promoted plant growth and metal resistance. The results suggested that the enhanced phytoextraction of Cd by Pennisetum hybridum with digestate application might mainly attributed to the increased Cd bio-availability and the enhanced plant growth, indicating that an approach combining digestate and Pennisetum hybridum could be a promising strategy for remediating Cd-contaminated soils.
Subject(s)
Pennisetum , Soil Pollutants , Biodegradation, Environmental , Cadmium/analysis , Soil , Soil Pollutants/analysisABSTRACT
Cadmium (Cd) contamination has become serious in soil and in situ stabilization technology has been widely used for heavy metal remediation. A field study was conducted to determine the effect of amendments with the doses of 3 kg/m2, including single vermicompost (A1), a 95% vermicompost mixed with 5% shell powder composite (A2) and a 95% vermicompost mixed with 5% modified shell powder composite (A3), on the Cd bioavailability, enzyme activity and bacterial community in soil, and the experiment was conducted with lettuce (Lactuca sativa L.) grown in a Cd-contaminated farmland soil. The results showed that the application of amendments increased the pH, cation exchange capacity (CEC), organic matter (OM), available nutrients, catalase (S-CAT), invertase (S-SC) and urease (S-UE) activities in soil, while significantly reduced the Cd bioavailability with the lowest Cd bioavailability being observed in the soil with A3 application. The soil bacterial richness and diversity increased after amendments application, and the bacterial community was characterized by an increase in metal-tolerant bacteria but a decrease in Proteobacteria, Acidobacteria and Gemmatimonadetes. In addition, the application of amendments significantly improved the growth of lettuce (Lactuca sativa L.) and inhibited Cd accumulation in its edible parts, especially, the Cd content in lettuce (Lactuca sativa L.) grown in soil with A3 application was below the limit of the National Food Safety Standard of China (maximum level ≤ 0.2 mg/kg). Thus, composite amendment obtained from vermicompost mixed with modified shell powder can be used as potential remediation material in Cd-contaminated soil. CAPSULE: Composite amendment obtained from vermicompost and modified shell powder had good effects on remediation of Cd-contaminated soil.
Subject(s)
Cadmium/analysis , Soil Microbiology , Soil Pollutants/analysis , Acidobacteria , Bacteria , Biological Availability , China , Environmental Pollution , Farms , Lactuca , Metals, Heavy , Powders , Proteobacteria , Soil/chemistry , UreaseABSTRACT
One of negative side-effects of usage of bio-renewables might be generation of mineral (ash) material, potential source of environmental pollution. A hypothesis was that bottom ash (BA; from biomass cogeneration facility) could be efficiently (re) used in soil chemical conditioning similarly to widely-used dolomite-based soil conditioner (DO; from Croatian Dinaric-coastal region) which we tested by: i) physicochemical characterisation of BA and DO, and ii) bioassay with Raphanus sativus cultivated in acidic soil amended with BA or DO. Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) confirmed complex chemical/physical structures and morphology between amendments, X-ray diffraction (XRD) showed their distinctive mineralogy with predominantly dolomite (in DO) vs. quartz and calcite (in BA), while secondary ion mass spectrometry (SIMS) revealed their diverse elemental/isotopic composition. The BA or DO amendments ameliorated soil acidity, increased available P, K and most other nutrients, but not Cd. The BA or DO amendments improved vegetative growth and edible hypocotyl yield. However, both amendments also increased Cd accumulation in all radish tissues, which was unexpected given the alkaline matrix of bio-ash and dolomite that would be likely to facilitate retention and immobilisation of toxic Cd. Thus, thorough characterisation and evaluation of BA- and/or DO-based materials and relevant soils (with an emphasis on metal sorption/immobilisation) prior to application in (agro) ecosystems is crucial for producing food clean of toxic metals.
Subject(s)
Raphanus , Soil Pollutants , Biomass , Cadmium/analysis , Calcium Carbonate , Coal Ash , Ecosystem , Magnesium , Nutrients , Soil , Soil Pollutants/analysisABSTRACT
Using diffusive gradients in thin films (DGT) is a recently developed alternative method of rapidly evaluating the bioavailability of metals in soil. However, the method has found only limited application in systematic assessment of the bioavailability of cadmium (Cd) in red limestone paddy soils treated with different soil amendments. Of the four methods compared for estimating Cd content of rice grains from plants grown in such soils of central China treated with eleven different soil amendments in pot culture, Cd content of DGT-labile soil was significantly correlated to Cd concentrations in brown rice (R = 0.447, p < 0.01). The other three methods involved CaCl2, diethylenetriaminepentaacetic acid (DTPA), or NH4NO3. Some other properties of soil, such as pH, redox potential, content of dissolved organic matter, and cation exchange capacity were also determined. A simple algorithm developed to evaluate the sensitivity of the four methods also confirmed DGT as the most efficient method to predict the bioavailability of Cd in red limestone paddy soils.