The potential for carbon dioxide removal by enhanced rock weathering in the tropics: An evaluation of Costa Rica.

Tropical environments show great potential to sequester CO2 by enhanced rock weathering (ERW) of powdered mafic rocks applied to agricultural fields. This study seeks to assess carbon dioxide reduction (CDR) potential in the humid tropics (1) by experimental weathering of mafic rock powders in conditions simulating humid tropical soils, and (2) from weathering rates determined from a Holocene tropical soil chronosequence where parent material is andesitic sediments. Experimentally determined weathering rates by leaching of basaltic andesites from Costa Rica (Arenal and Barva) for 50 t ha-1 applications indicate potential sequestration of 2.4 to 4.5 t CO2 ha-1 yr-1, whereas the USGS basalt standard BHVO-1 yields a rate of 11.9 t ha-1 yr-1 (influenced by more mafic composition and finer particle size). The chronosequence indicates a rate of 1.7 t CO2 ha-1 yr-1. The weathering experiment consisted of 0.6 mm of powdered rock applied atop 12 mm of Ultisol at 35 °C. To simulate a tropical soil solution, 100-mL aliquots of a dilute solution of oxalic acid in carbonated DI water were rained onto soils over a 14-day period to simulate soil moisture in the humid tropics. Solutions were collected and analyzed by ICPMS for concentrations of leached cations. A potential ERW scenario for Costa Rica was assessed assuming that one-half of lowland agricultural kaolinitic soils (mainly Ultisols, common crop and pasture soils, excluding protected areas) were to receive 50 t ha-1 of annual or biennial applications of powdered mafic rock. With an experimentally determined humid tropical CDR rate for basaltic andesite (3.5 t ha-1 yr-1) and allowances for carbon costs (e.g. emissions from processing and delivery) that reduce CDR to a net 3.2 t ha-1 yr-1, potential annual CDR of this tropical nation is ∼2-4 million tons, amounting to ∼25-50 % of annual CO2 emissions (mainly from transportation in Costa Rica).

Application of films developed with tilapia gelatin (Oreochromis niloticus), added with pitomba plant extract (Talisia esculenta) in Hawaii papaya.

In the present study, a film based on the gelatin skin of tilapia (Oreochromis niloticus) was developed, using surfactants and adding plant extract of pitomba seed (Talisia esculenta). The aim was to investigate the mechanical and barrier properties of the cover, as well as its effectiveness in conserving papayas against diseases caused by fungi. The film presented tensile strength of 38.78 MPa, elongation of 120.49%, and water vapor permeability of 5.90 g.mm.h-1.m2.kPa-1 when equally composed of SDS and Tween 80, in a percentage of 40% in relation to the total mass of the film. The films lasted 12 d in an environment with a relative humidity of 75% (25 ºC), longer than the shelf life of papaya (limited to 8 d). With applying the film with the extract, the incidence of diseases such as anthracnose, fusariosis, and stem rot caused by these microorganisms in papaya was reduced.

SPCIS: Standardized Plant Community with Introduced Status database.

The movement of plant species across the globe exposes native communities to new species introductions. While introductions are pervasive, two aspects of variability underlie patterns and processes of biological invasions at macroecological scales. First, only a portion of introduced species become invaders capable of substantially impacting ecosystems. Second, species that do become invasive at one location may not be invasive in others; impacts depend on invader abundance and recipient species and conditions. Accounting for these phenomena is essential to accurately understand the patterns of plant invasion and explain the idiosyncratic results reflected in the literature on biological invasions. The lack of community-level richness and the abundance of data spanning broad scales and environmental conditions have until now hindered our understanding of invasions at a macroecological scale. To address this limitation, we leveraged quantitative surveys of plant communities in the USA and integrated and harmonized nine datasets into the Standardized Plant Community with Introduced Status (SPCIS) database. The database contains 14,056 unique taxa identified within 83,391 sampling units, of which 52.6% have at least one introduced species. The SPCIS database includes comparable information on plant species occurrence, abundance, and native status across the 50 U.S. States and Puerto Rico. SPCIS can be used to answer macro-scale questions about native plant communities and interactions with invasive plants. There are no copyright restrictions on the data, and we ask the users of this dataset to cite this paper, the respective paper(s) corresponding to the dataset sampling design (all references are provided in Data S1: Metadata S1: Class II-B-2), and the references described in Data S1: Metadata S1: Class III-B-4 as applicable to the dataset being utilized.

Mechanisms underlying interaction frequencies and robustness in a novel seed dispersal network: lessons for restoration.

As human-caused extinctions and invasions accumulate across the planet, understanding the processes governing ecological functions mediated by species interactions, and anticipating the effect of species loss on such functions become increasingly urgent. In seed dispersal networks, the mechanisms that influence interaction frequencies may also influence the capacity of a species to switch to alternative partners (rewiring), influencing network robustness. Studying seed dispersal interactions in novel ecosystems on O'ahu island, Hawai'i, we test whether the same mechanisms defining interaction frequencies can regulate rewiring and increase network robustness to simulated species extinctions. We found that spatial and temporal overlaps were the primary mechanisms underlying interaction frequencies, and the loss of the more connected species affected networks to a greater extent. Further, rewiring increased network robustness, and morphological matching and spatial and temporal overlaps between partners were more influential on network robustness than species abundances. We argue that to achieve self-sustaining ecosystems, restoration initiatives can consider optimal morphological matching and spatial and temporal overlaps between consumers and resources to maximize chances of native plant dispersal. Specifically, restoration initiatives may benefit from replacing invasive species with native species possessing characteristics that promote frequent interactions and increase the probability of rewiring (such as long fruiting periods, small seeds and broad distributions).

Humpback whales (Megaptera novaeangliae) attend both Mexico and Hawaii breeding grounds in the same winter: mixing in the northeast Pacific.

Humpback whales that assemble on winter breeding grounds in Mexico and Hawaii have been presumed to be, at least, seasonally isolated. Recently, these assemblies were declared Distinct Population Segments under the US Endangered Species Act. We report two humpback whales attending both breeding grounds in the same season-one moving from Hawaii to Mexico and the other from Mexico to Hawaii. The first was photo-identified in Maui, Hawaii on 23 February 2006 and again, after 53 days and 4545 km, on 17 April 2006 in the Revillagigedo Archipelago, Mexico. The second was photo-identified off Guerrero, Mexico on 16 February 2018 and again, 49 days and 5944 km later, on 6 April 2018 off Maui. The 2006 whale was identified in summer off Kodiak Island, Alaska; the 2018 whale off British Columbia. These Mexico-Hawaii identifications provide definitive evidence that whales in these two winter assemblies may mix during one winter season. This, combined with other lines of evidence on Mexico-Hawaii mixing, including interchange of individuals year to year, long-term similarity of everchanging songs, one earlier same-season travel record, and detection of humpback whales mid-ocean between these locations in winter, suggests reassessment of the 'distinctiveness' of these populations may be warranted.

Meteorological and Air Quality Modeling for Hawaii, Puerto Rico, and Virgin Islands.

A photochemical model platform for Hawaii, Puerto Rico, and Virgin Islands predicting O3, PM2.5, and regional haze would be useful to support assessments relevant for the National Ambient Air Quality Standards (NAAQS), Regional Haze Rule, and the Prevention of Significant Deterioration (PSD) program. These areas have not traditionally been modeled with photochemical transport models, but a reasonable representation of meteorology, emissions (natural and anthropogenic), chemistry, and deposition could support air quality management decisions in these areas. Here, a prognostic meteorological model (Weather Research and Forecasting) and photochemical transport (Community Multiscale Air Quality) model were applied for the entire year of 2016 at 27, 9, and 3 km grid resolution for areas covering the Hawaiian Islands and Puerto Rico/Virgin Islands. Model predictions were compared against surface and upper air meteorological and chemical measurements available in both areas. The vertical gradient of temperature, humidity, and winds in the troposphere was well represented. Surface layer meteorological model performance was spatially variable, but temperature tended to be underestimated in Hawaii. Chemically speciated daily average PM2.5 was generally well characterized by the modeling system at urban and rural monitors in Hawaii and Puerto Rico/Virgin Islands. Model performance was notably impacted by the wildfire emission methodology. Model performance was mixed for hourly SO2, NO2, PM2.5, and CO and was often related to how well local emissions sources were characterized. SO2 predictions were much lower than measurements at monitors near active volcanos on Hawaii, which was expected since volcanic emissions were not included in these model simulations. Further research is needed to assess emission inventory representation of these areas and how microscale meteorology influenced by the complex land-water and terrain interfaces impacts higher time resolution performance.

Historical Migration revealed through a Case of Autosomal Dominant Alzheimer's Disease.

With the increasing geopolitical instability and environmental devastation occurring across the globe, human migration is increasing. We report a case that illustrates a migration pattern seen a century ago that is currently in the process of repeating itself. Using information from a neuropathological examination, genetic analyses, and historical sources, we linked a patient with autosomal dominant Alzheimer's disease in Hawai'i with her ancestors in Puerto Rico. In this patient we identified the G206A PSEN1 mutation, previously identified as being linked to a founder effect from Puerto Rico. At the turn of the twentieth century, due to devastating hurricanes in Puerto Rico and the island's new status as a possession of the United States, over 5,000 Puerto Ricans, including the grandparents of our patient, migrated to Hawai'i. This short-term but historic migration has resulted in a significant population of Puerto Ricans in Hawai'i, today. As physicians we sometimes have the opportunity and privilege, through the patients who come to us for help, to be indirect witnesses to such historical events and movements. These occurrences can inform the present and also portend future developments in this rapidly changing world.

SNPs across time and space: population genomic signatures of founder events and epizootics in the House Finch (Haemorhous mexicanus).

Identifying genomic signatures of natural selection can be challenging against a background of demographic changes such as bottlenecks and population expansions. Here, we disentangle the effects of demography from selection in the House Finch (Haemorhous mexicanus) using samples collected before and after a pathogen-induced selection event. Using ddRADseq, we genotyped over 18,000 SNPs across the genome in native pre-epizootic western US birds, introduced birds from Hawaii and the eastern United States, post-epizootic eastern birds, and western birds sampled across a similar time span. We found 14% and 7% reductions in nucleotide diversity, respectively, in Hawaiian and pre-epizootic eastern birds relative to pre-epizootic western birds, as well as elevated levels of linkage disequilibrium and other signatures of founder events. Despite finding numerous significant frequency shifts (outlier loci) between pre-epizootic native and introduced populations, we found no signal of reduced genetic diversity, elevated linkage disequilibrium, or outlier loci as a result of the epizootic. Simulations demonstrate that the proportion of outliers associated with founder events could be explained by genetic drift. This rare view of genetic evolution across time in an invasive species provides direct evidence that demographic shifts like founder events have genetic consequences more widespread across the genome than natural selection.

Evaluating localism in the management of post-consumer plastic bottles in Honolulu, Hawai'i: perspectives from industrial ecology and political ecology.

Localism or regionalization has become a popular topic in urban design, but recent critics raise the question of whether the local or regional scale is most desirable for industrial ecosystems. As a way to explore the claim that localized metabolism is more sustainable, this study examines the costs and benefits of two differentially scaled strategies for the management of post-consumer polyethylene terephthalate (PET) bottles originating in the city of Honolulu, Hawai'i: local incineration and trans-continental recycling. We first estimate total environmental impacts of two options using life cycle assessment, and then disaggregate them into local versus non-local impacts to examine the spatial distribution of costs and benefits. We further assess the environmental justification for localized waste management in relation to the broader socio-economic motivations that underlie the way that plastics are managed in Honolulu. In doing so we assess the scale at which waste management is optimized from an environmental standpoint as well as the non-environmental considerations such as security and safety that influence the politics of scale involved in urban metabolic design. By illustrating the trade-offs between a local versus global metabolic pathway for plastic waste, the results from our Honolulu case study are globally relevant for communities interested in sustainable urban design and in particular urban waste management.

Incorrect handling of calibration information in divergence time inference: an example from volcanic islands.

Divergence time studies rely on calibration information from several sources. The age of volcanic islands is one of the standard references to obtain chronological data to estimate the absolute times of lineage diversifications. This strategy assumes that cladogenesis is necessarily associated with island formation, and punctual calibrations are commonly used to date the splits of endemic island species. Here, we re-examined three studies that inferred divergence times for different Hawaiian lineages assuming fixed calibration points. We show that, by permitting probabilistic calibrations, some divergences are estimated to be significantly younger or older than the age of the island formation, thus yielding distinct ecological scenarios for the speciation process. The results highlight the importance of using calibration information correctly, as well as the possibility of incorporating volcanic island studies into a formal, biogeographical hypothesis-testing framework.

BRASILONEMA ANGUSTATUM SP. NOV. (NOSTOCALES), A NEW FILAMENTOUS CYANOBACTERIAL SPECIES FROM THE HAWAIIAN ISLANDS(1).

A new filamentous cyanobacterial species of the genus Brasilonema was isolated from the island of Oahu, Hawaii. The taxon is distinguished from the seven other species in the genus by attenuation of trichomes, and is here described as Brasilonema angustatum sp. nov. It possesses the cytoplasmic kerotimization and reddish-brown coloration of several species in the genus. Phylogenetic analysis based on 16S rRNA gene sequence shows B. angustatum within a highly supported clade containing all sequenced Brasilonema species. We compared the secondary structure of the 16S-23S ITS regions for B. octagenarum and B. angustatum. The structurally conservative D1-D1' and V3 helices show similar motifs between the two taxa, but differ structurally and in sequence, providing additional justification for erection of the new species. The Box-B helix has identical secondary structure. The existence of tapering in a Brasilonema species is unique in this genus, and requires modification of the current concept of the genus Brasilonema, which was described as being unattenuated. Our phylogenetic evidence supports the hypothesis that tapering has developed repeatedly in separate cyanobacterial lineages and lacks the taxonomic significance once assumed by early workers.

Initial growth of Carica papaya under irrigation with saline water in soil with bovine biofertilizer / Crescimento inicial de Carica papaya sob irrigação com águas salinas em solo com biofertilizante bovino

The salinity is considered the major constraint to agriculture worldwide, constituting a limiting factor to growth, plant development, agricultural productivity and soil deterioration. In this direction an experiment was carried out during the period October/2009 to February/2010, in greenhouse conditions in Areia county, Paraiba State, PB, Brazil, in order to evaluate the effects of water saline on initial growth of papaya Hawaii in non-saline substrate with bovine rich biofertilizer. The substrate was material of the first 0.10 m of a Regolitic Entisol non saline. The treatments were distributed in completely randomized in six replication using the factorial design 5 x 2, corresponding to levels of irrigation water saline: 0.5; 1.0; 2.0; 3.0 and 4.0 dS m-1, in soil without and with rich biofertilizer applied to soil in liquid form one time two days before seed sowing, at level of 10% of the substrate volume. The increment of salinity water irrigation inhibited alls variables studied in papayas plants, but with less range in treatments with bovine biofertilizer.

A salinidade é considerada um dos principais entraves para agricultura em todo mundo, constituindose num dos fatores limitantes ao crescimento, desenvolvimento das plantas, produtividade agrícola e depauperamento do solo. Nesse sentido, um experimento foi desenvolvido no período de Outubro de 2009 a Fevereiro de 2010, em ambiente telado, no município de Areia PB, para avaliar a influência da salinidade da água de irrigação no crescimento do mamão Havaí em substrato não salino com biofertilizante rico. O substrato utilizado foi o material dos primeiros 0,10 m de um NEOSSOLO REGOLITICO não salino. O delineamento experimental foi inteiramente casualizado em esquema fatorial 5 x 2, referente aos valores de condutividade elétrica da água de irrigação: 0,5; 1,0; 2,0; 3,0 e 4,0 dS m-1, em solo sem e com biofertilizante líquido, com seis repetições. O biofertilizante rico, depois de diluído em água não salina (0,5 dS m-1) na razão de 1:1 foi aplicado uma única vez, dois dias antes da semeadura, em nível de 10% do volume do substrato. O aumento da salinidade da água de irrigação provocou perdas sobre todas as variáveis avaliadas nas plantas, mas com menor intensidade nos tratamentos com biofertilizante.

Initial growth of Carica papaya under irrigation with saline water in soil with bovine biofertilizer / Crescimento inicial de Carica papaya sob irrigação com águas salinas em solo com biofertilizante bovino

The salinity is considered the major constraint to agriculture worldwide, constituting a limiting factor to growth, plant development, agricultural productivity and soil deterioration. In this direction an experiment was carried out during the period October/2009 to February/2010, in greenhouse conditions in Areia county, Paraiba State, PB, Brazil, in order to evaluate the effects of water saline on initial growth of papaya Hawaii in non-saline substrate with bovine rich biofertilizer. The substrate was material of the first 0.10 m of a Regolitic Entisol non saline. The treatments were distributed in completely randomized in six replication using the factorial design 5 x 2, corresponding to levels of irrigation water saline: 0.5; 1.0; 2.0; 3.0 and 4.0 dS m-1, in soil without and with rich biofertilizer applied to soil in liquid form one time two days before seed sowing, at level of 10% of the substrate volume. The increment of salinity water irrigation inhibited alls variables studied in papayas plants, but with less range in treatments with bovine biofertilizer. 

A salinidade é considerada um dos principais entraves para agricultura em todo mundo, constituindose num dos fatores limitantes ao crescimento, desenvolvimento das plantas, produtividade agrícola e depauperamento do solo. Nesse sentido, um experimento foi desenvolvido no período de Outubro de 2009 a Fevereiro de 2010, em ambiente telado, no município de Areia PB, para avaliar a influência da salinidade da água de irrigação no crescimento do mamão Havaí em substrato não salino com biofertilizante rico. O substrato utilizado foi o material dos primeiros 0,10 m de um NEOSSOLO REGOLITICO não salino. O delineamento experimental foi inteiramente casualizado em esquema fatorial 5 x 2, referente aos valores de condutividade elétrica da água de irrigação: 0,5; 1,0; 2,0; 3,0 e 4,0 dS m-1, em solo sem e com biofertilizante líquido, com seis repetições. O biofertilizante rico, depois de diluído em água não salina (0,5 dS m-1) na razão de 1:1 foi aplicado uma única vez, dois dias antes da semeadura, em nível de 10% do volume do substrato. O aumento da salinidade da água de irrigação provocou perdas sobre todas as variáveis avaliadas nas plantas, mas com menor intensidade nos tratamentos com biofertilizante. 

Assessment of Acacia koa forest health across environmental gradients in Hawai'i using fine resolution remote sensing and GIS.

Koa (Acacia koa) forests are found across broad environmental gradients in the Hawai'ian Islands. Previous studies have identified koa forest health problems and dieback at the plot level, but landscape level patterns remain unstudied. The availability of high-resolution satellite images from the new GeoEye1 satellite offers the opportunity to conduct landscape-level assessments of forest health. The goal of this study was to develop integrated remote sensing and geographic information systems (GIS) methodologies to characterize the health of koa forests and model the spatial distribution and variability of koa forest dieback patterns across an elevation range of 600-1,000 m asl in the island of Kaua'i, which correspond to gradients of temperature and rainfall ranging from 17-20 °C mean annual temperature and 750-1,500 mm mean annual precipitation. GeoEye1 satellite imagery of koa stands was analyzed using supervised classification techniques based on the analysis of 0.5-m pixel multispectral bands. There was clear differentiation of native koa forest from areas dominated by introduced tree species and differentiation of healthy koa stands from those exhibiting dieback symptoms. The area ratio of healthy koa to koa dieback corresponded linearly to changes in temperature across the environmental gradient, with koa dieback at higher relative abundance in warmer areas. A landscape-scale map of healthy koa forest and dieback distribution demonstrated both the general trend with elevation and the small-scale heterogeneity that exists within particular elevations. The application of these classification techniques with fine spatial resolution imagery can improve the accuracy of koa forest inventory and mapping across the islands of Hawai'i. Such findings should also improve ecological restoration, conservation and silviculture of this important native tree species.

Genetic population structure of an anchialine shrimp, Metabetaeus lohena (Crustacea: Alpheidae), in the Hawaiian Islands

Anchialine habitats in the Hawaiian Islands, characterized as coastal bodies of land-locked salt or brackish water that fluctuate with the tides due to subterranean connections, are the only ecosystems of this type found within the United States. These habitats are currently subject to anthropogenic impacts tha t threaten their future existence. Previous research has shown strong genetic population structure of an endemic atyid shrimp, Halocaridina rubra, in these habitats. The native alpheid shrimp, Metabetaeus lohena, whose known range entirely overlaps that of H. rubra, has feeding and reproductive behaviors that are biologically distinct from H. rubra. Its historic scarcity and status as a candidate for the US Fish and Wildlife Department’s Endangered Species List, make M. lohena an ideal species to compare against the known genetic structure of H. rubra. We investigated the population structure of this native anchialine shrimp to test the hypothesis that genetic population structure differs between the two shrimp species and that M. lohena is genetically unstructured across its range. A survey of 605 bp of the mitochondrial cytochrome c oxidase subunit I (COI) gene from 127 individuals collected at 7 sites spanning the islands of O’ahu, Maui and Hawaii revealed 43 haplotypes. The most common haplotype was represented in similar proportions from all sites sampled, accounting for 44% of the surveyed sequences. Analyses of molecular variation (AMOVA), pairwise ΦST values, Bayesian estimates of migration (M), Mantel tests and Nested Clade Analyses (NCAs) all failed to reveal evidence of major barriers to gene flow among most populations separated by inter-island channels. This lack of genetic structure in M. lohena is found to be in stark contrast with the highly structured population of H. rubra, and may be attributed to oceanic dispersal strategies and/or a recent introduction to the Hawaiian Islands. Rev. Biol. Trop. 58 (1): 159-170. Epub 2010 March 01.

Los hábitats de los alfeidos de las islas de Hawaii, se caracterizan por ser zonas cerradas de aguas saladas o salobres, que fluctúan con las mareas, debido a las conexiones subterráneas, son los únicos ecosistemas de este tipo que se encuentran en Estados Unidos. Estos hábitats actualmente están sujetos a impactos antropogénicos que amenazan su existencia futura. La investigación anterior ha demostrado una fuerte estructura genética de una población de camarones atíidos endémicos, Halocaridina rubra, en estos hábitats. El camarón alfeido nativo, Metabetaeus lohena, cuya área de distribución conocida se superpone totalmente con la de H. rubra, tiene comportamientos alimenticios y reproductivos que son biológicamente diferentes a los de H. rubra. Su escasez histórica y su condición de candidato para aparecer en la Lista de Especies en Peligro del Departamento de Pesca y Vida Silvestre de Estados Unidos, hace de M. lohena una especie ideal para comparar su estructura genética con la de H. rubra. Se investigó la estructura de la población de este camarón alfeido nativo para probar la hipótesis que la estructura genética de la población difiere entre las dos especies y que la de M. lohena está genéticamente no estructurada en todo su ámbito. El análisis de 605 pb de la oxidasa mitocondrial citocromo c subunidad I (COI) de genes de 127 individuos recolectados en 7 sitios que abarcan las islas de Oahu, Maui y Hawaii reveló 43 haplotipos. El haplotipo más común fue representado en proporciones similares en todos los sitios incluidos en la muestra, de acuerdo al 44% de las secuencias estudiadas. El análisis de variación molecular (AMOVA), los valores de ΦST pareados, la estimación bayesiana de la migración (M), las pruebas de Mantel y los Análisis Cladísticos no pudieron revelar la existencia de importantes barreras al flujo genético entre las poblaciones más separadas por los canales entre las islas. La falta de estructura genética en M. lohena contrasta con la muy estructurada población de H. rubra, y puede ser atribuida a las estrategias de dispersión oceánica y/o una introducción reciente en las islas hawaianas.