The effect of group size on sleep in a neotropical bat, Artibeus jamaicensis.

Sleep is associated with many costs, but is also important to survival, with a lack of sleep impairing cognitive function and increasing mortality. Sleeping in groups could alleviate sleep-associated costs, or could introduce new costs if social sleeping disrupts sleep. Working with the Jamaican fruit bat (Artibeus jamaicensis), we aimed to: (1) describe sleep architecture, (2) assess how sleeping in groups affects sleep, and (3) quantify total sleep time and identify rapid eye movement (REM) sleep using behavioral indicators that complement physiological evidence of sleep. Twenty-five adult bats were captured in Panama and recorded sleeping in an artificial roost enclosure. Three bats were fitted with an electromyograph and accelerometer and video recorded sleeping alone in controlled laboratory settings. The remaining 22 bats were assigned to differing social configurations (alone, dyad, triad, and tetrad) and video recorded sleeping in an outdoor flight cage. We found that sleep was highly variable among individuals (ranging from 2 h 53 min to 9 h 39 min over a 12-h period). Although we did not detect statistically significant effects and our sample size was limited, preliminary trends suggest that male bats may sleep longer than females, and individuals sleeping in groups may sleep longer than individuals sleeping alone. We also found a high correspondence between total sleep time quantified visually and quantified using actigraphy (with a 2-min immobility threshold) and identified physiological correlates of behaviorally-defined REM. These results serve as a starting point for future work on the ecology and evolution of sleep in bats and other wild mammals.

Dormir está asociado con muchos costos, pero también es importante para la supervivencia, ya que la falta de sueño perjudica la función cognitiva y aumenta la mortalidad. Dormir en grupos podría disminuir los costos asociados con el sueño o podría introducir nuevos costos si este descanso grupal interrumpe el sueño. Trabajando con el murciélago frugívoro jamaicano (A. jamaicensis), nos propusimos: (1) describir la arquitectura del sueño, (2) evaluar cómo dormir en grupos afecta el sueño y (3) cuantificar el tiempo total de sueño e identificar el sueño REM empleando indicadores de comportamiento que complementen la evidencia fisiológica del sueño. Se capturaron 25 murciélagos adultos en Panamá y se les grabó durmiendo en un recinto artificial de descanso. A tres murciélagos se les equipó con un electromiógrafo y un acelerómetro y se les grabó en video durmiendo solos en entornos de laboratorio controlados. Los 22 murciélagos restantes fueron asignados a diferentes configuraciones sociales (solo, díada, tríada, tétrada) y se grabaron en video durmiendo en una jaula de vuelo al aire libre. El sueño fue muy variable entre individuos (desde 2 h 53 min hasta 9 h 39 min durante un período de 12 h). Aunque no detectamos efectos estadísticamente significativos, y nuestro tamaño muestral fue limitado, las tendencias preliminares sugieren que los murciélagos macho pueden dormir más que las hembras, y que los individuos que duermen en grupos pueden dormir más que los individuos que duermen solos. Encontramos una alta correspondencia entre el tiempo total de sueño cuantificado visualmente y cuantificado mediante actigrafía (con un umbral de inmovilidad de 2 minutos) e identificamos correlaciones fisiológicas de lo que definimos como REM en términos de comportamiento. Estos resultados sirven como punto de partida para futuros estudios sobre la ecología y la evolución del sueño en murciélagos y otros mamíferos salvajes.

Group membership, not diet, structures the composition and functional potential of the gut microbiome in a wild primate.

The gut microbiome has the potential to buffer temporal variations in resource availability and consumption, which may play a key role in the ability of animals to adapt to a broad range of habitats. We investigated the temporal composition and function of the gut microbiomes of wild common marmosets (Callithrix jacchus) exploiting a hot, dry environment-Caatinga-in northeastern Brazil. We collected fecal samples during two time periods (July-August and February-March) for 2 years from marmosets belonging to eight social groups. We used 16S rRNA gene amplicon sequencing, metagenomic sequencing, and butyrate RT-qPCR to assess changes in the composition and potential function of their gut microbiomes. Additionally, we identified the plant, invertebrate, and vertebrate components of the marmosets' diet via DNA metabarcoding. Invertebrate, but not plant or vertebrate, consumption varied across the year. However, gut microbiome composition and potential function did not markedly vary across study periods or as a function of diet composition. Instead, the gut microbiome differed markedly in both composition and potential function across marmosets residing in different social groups. We highlight the likely role of factors, such as behavior, residence, and environmental heterogeneity, in modulating the structure of the gut microbiome. IMPORTANCE: In a highly socially cohesive and cooperative primate, group membership more strongly predicts gut microbiome composition and function than diet.

Influences of sociality and maternal size on reproductive strategies: trade-offs between offspring size and quantity in five Anelosimus species (Araneae, Theridiidae).

Individuals can experience accentuated disputes for resources when living with many conspecifics, even in situations in which cooperative behaviors assure benefits associated with an increase in the frequency of food acquisition and in diet breadth. Thus, intraspecific competition may exert a significant selective pressure on social animals. Theoretical models suggest that females of social species could improve their fitness by producing relatively large offspring, since body size can provide competitive advantages during foraging activities. As female reserves are limited, the production of large offspring would occur at the expense of their number. Using five Anelosimus (Araneae, Theridiidae) species, we assessed whether the social ones produce fewer and larger eggs than the subsocials. In addition, we tested the effect of female size on the adoption of each particular reproductive strategy. Small females could hypothetically invest in producing large offspring since they cannot produce as many offspring as large females. Our results suggested that, indeed, sociality influences reproductive strategies. Females of social species produced fewer and larger offspring than females of subsocial species. Subsociality, in turn, would benefit the production of many small spiderlings, possibly because a large number of siblings is important to maintain and expand new webs and to subdue prey during their initial instars. Our results also indicated that large females produce more eggs without necessarily reduce their sizes. We discussed how the costs and benefits of group living may influence reproductive strategies.

Ayahuasca Ritual, Personality and Sociality: Observational Research Conducted in a Substance Use Disorder Rehabilitation Center in Uruguay.

The current article presents a mixed qualitative-quantitative observational study of the effect of ayahuasca ritual on subjective experiences and personality traits on participants of a center specialized in the treatment of substance use disorder in Uruguay. When comparing the psychological traits of ayahuasca participants to a control group, quantitative results using the Zuckerman-Kuhlman-Aluja Personality Questionnaire showed statistically significant higher scores in Impulsive Sensation Seeking, Boredom Susceptibility, and Social Warmth scales. Qualitative analysis of ayahuasca experiences resulted in five main categories: emotional experiences (including social emotions such as love and empathy), corporal experiences, spiritual/transcendental experiences, personal experiences, and visions. Last, qualitative descriptions provide support for the importance of social interactions in the phenomenological manifestations of the psychedelic experience. Both quantitative and qualitative results suggest that the combination of social interactions and the pharmacological action of ayahuasca could facilitate the manifestation of social emotions during the ritual, and may contribute to the long-term increase of empathic and social aspects of personality.

Kleptoparasites of social spider colonies do not track hosts' subdivided population structure.

Organisms with lower dispersal abilities tend to have more genetically dissimilar populations. The same is true for parasites, whose transmission frequency may depend on the population structure of the host. This should be especially true when hosts and parasites face similar barriers to dispersal. Here, we considered the similarities between host and parasite population structure in a social spider system. In this system, host colonies are typified by rapid growth via internal recruitment followed by budding or fission events when colonies grow too large, with each colony representing a distinct population. Host colonies provide habitat for kleptoparasitic spiders, which steal prey from and may also feed directly on host individuals. We asked whether kleptoparasites exhibit a similar degree of population subdivision as their host. Under the free-mixing hypothesis (i.e., horizontal transmission), kleptoparasites would have well-mixed populations across broader regions than a single host nest, whereas host populations would be strongly genetically structured. Under the host-tracking hypothesis (i.e., vertical transmission), kleptoparasites would have a population structure that parallels that of the host. We conducted a genotype-by-sequencing study to assess the population structure of both hosts and kleptoparasites within three nearby regions in eastern Ecuador. We found strong signatures of population differentiation and bottlenecks in the host species, which is congruent with past studies. However, we found that kleptoparasite populations were well mixed across host nests, with no evidence of recent bottlenecks. These results support our free-mixing hypothesis, suggesting that kleptoparasites follow patterns of horizontal transmission in this social spider system.

Distinguishing Intergroup and Long-Distance Relationships.

Intergroup and long-distance relationships are both central features of human social life, but because intergroup relationships are emphasized in the literature, long-distance relationships are often overlooked. Here, we make the case that intergroup and long-distance relationships should be studied as distinct, albeit related, features of human sociality. First, we review the functions of both kinds of relationship: while both can be conduits for difficult-to-access resources, intergroup relationships can reduce intergroup conflict whereas long-distance relationships are especially effective at buffering widespread resource shortfalls. Second, to illustrate the importance of distinguishing the two relationship types, we present a case study from rural Bolivia. Combining ethnography and two different experimental techniques, we find that the importance of intergroup relationships-and the salience of group membership itself-varies across populations and across methods. Although ethnography revealed that participants often rely on long-distance relationships for resource access, we were unable to capture participant preferences for these relationships with a forced-choice technique. Taken together, our review and empirical data highlight that (1) intergroup and long-distance relationships can have different functions and can be more or less important in different contexts and (2) validating experimental field data with ethnography is crucial for work on human sociality. We close by outlining future directions for research on long-distance relationships in humans.

Na batida da corda, afetos e desejos: grupos de pessoas que vivem com HIV/aids no WhatsApp e a produção de socialidades / At the rhythm of the skipping rope, affections and desires: groups of people living with HIV/AIDS on WhatsApp and the production of socialities / En el ritmo de saltar la cuerda, afectos y deseos: grupos de personas que viven con VIH/SIDA en WhatsApp y la producción de sociabilidades

Neste artigo são delineadas reflexões teórico-etnográficas a partir de pesquisa de campo realizada em três grupos do WhatsApp formados por pessoas que vivem com HIV/aids (PVHA). Tem como objetivo compreender as experiências afetivo-sexuais dessas pessoas, construídas por meio de diálogos, interações e socialidades vividas nesses grupos pesquisados. A partir da descrição das socialidades nos grupos e dos modos como, por meio da biscoitagem, as pessoas fazem circular afetos e desejos sem, necessariamente, constituírem relacionamentos afetivo-sexuais, argumenta-se que, em suas interações sociais, os interlocutores conectavam pessoas, artefatos sociotécnicos, lugares, situações, emoções, relações, projetos de vida etc., de tal maneira que produziam mais que simplesmente apoio, suporte, ajuda ou comunicação sobre o HIV, visto que teciam seus próprios mundos sociais. Estes achados ganham relevância especial ao se considerar a experiência de PVHA que mantêm algum grau de segredo sobre sua sorologia positiva para o HIV

This article outlines theoretical-ethnographic reflection based on a field research about three WhatsApp groups formed by people living with HIV/AIDS (PLWHA). It aims to understand the affective-sexual experiences of these people constructed through dialogues, interactions and socialities on these WhatsApp groups. From the description of sociality in these groups and the ways in which, through biscoitagem, people circulated affections and desires without necessarily constituting affective-sexual relationships, it is argued that, in their social interactions, the interlocutors connected people, socio-technical artefacts, places, situations, emotions, relationships, life projects etc., in a way that produced more than support, mutual help or communication about HIV, insofar as they were weaving their own social worlds. These findings gain special relevance when we consider the experience of the PLWHA who maintain some degree of secrecy about their positive HIV serology

Este artículo esboza reflexiones teórico-etnográficas a partir de una investigación de campo en tres grupos en WhatsApp formados por personas viviendo con VIH/SIDA (PVVS). El objetivo es comprender las experiencias afectivo-sexuales de estas personas construidas a través de diálogos, interacciones y socialidades vividas en estos grupos. A partir de la descripción de la sociabilidad en los grupos investigados y de las formas en que, a través de la biscoitagem, las personas circulaban afectos y deseos sin constituir necesariamente relaciones afectivo-sexuales, se argumenta que, en sus interacciones sociales, los interlocutores conectaron personas, artefactos sociotécnicos, lugares, situaciones, emociones, relaciones, proyectos de vida etc., de manera que produjeron más que solamente apoyo, soporte, ayuda o comunicación sobre VIH, ya que tejian sus propios mundos sociales. Estos hallazgos adquieren especial relevancia cuando se considera la experiencia de las PVVS que mantienen cierto grado de secreto sobre su serología positiva para el VIH

The Effect of Diet on Colony Recognition and Cuticular Hydrocarbon Profiles of the Invasive Argentine Ant, Linepithema humile.

Ants are some of the most abundant and ecologically successful terrestrial organisms, and invasive ants rank among the most damaging invasive species. The Argentine ant is a particularly well-studied invader, in part, because of the extreme social structure, known as unicoloniality, that occurs in introduced populations. Unicoloniality is characterized by the formation of geographically vast supercolonies, within which territorial behavior and intraspecific aggression are absent. Although there is considerable evidence supporting a genetic basis for the odor cues involved in colony recognition, some studies have suggested that diet may also influence colony recognition cues and, thus, colony structure. Here, we test the role for insect-derived recognition cues by performing a diet supplementation experiment in a natural field setting, and a more extreme dietary manipulation experiment in the lab. After one month, in both the field and the lab, we found that aggressive supercolonies remained aggressive toward each other and non-aggressive nests (from the same supercolony) remained non-aggressive, regardless of dietary treatment. In one lab treatment, we did observe a significant decrease in the level of aggression between different supercolonies that were fed the same diet, but aggression was still frequent. We did not see any evidence for cuticular hydrocarbon odor cues being transferred from prey to ants in any of the field treatments. In the more extreme lab treatment, however, several cuticular hydrocarbons were acquired from both roach and cricket insect prey (but not Drosophila). Based on these data, we conclude that dietary changes are unlikely to underlie changes in behavior or colony structure in Argentine ants in real-world settings. However, these results indicate that caution is warranted when interpreting the behaviors of animals that have been reared on diets that are substantially different from natural populations.

Sex, not social behavior, predicts fecal glucocorticoid metabolite concentrations in a facultatively social rodent, the highland tuco-tuco (Ctenomys opimus).

Social relationships may influence circulating glucocorticoid levels, particularly in group-living species in which individuals regularly engage in interactions with conspecifics. The effects of such interactions appear to vary, with greater social contact being associated with increased glucocorticoid concentrations in some species but decreased concentrations in others. These distinct responses raise intriguing questions regarding relationships among social behavior, individual phenotypes, and glucocorticoid physiology. To explore such relationships in a free-living mammal with a dynamic social organization, we quantified variation in baseline glucocorticoids in a population of highland tuco-tucos (Ctenomys opimus) from Jujuy Province, Argentina. These subterranean rodents are facultatively social, with lone and group-living individuals regularly occurring within the same population. To assess potential endocrine correlates of this behavioral variability, we examined differences in baseline fecal glucocorticoid metabolite (fGCm) concentrations as a function of social group size and composition as well as several metrics of social behavior derived from social network analyses. Despite marked variability in social relationships among the 37 (12 male, 25 female) free-living tuco-tucos sampled, none of the measures of social behavior examined were significant predictors of variation in fGCm concentrations. In contrast, individual variation in glucocorticoid metabolites was best explained by sex, with males having higher fGCm concentrations than females. These analyses provide the first characterization of the glucocorticoid physiology of highland tuco-tucos and underscore the potential importance of intrinsic phenotypic factors (e.g., sex) in shaping glucocorticoid variation in free-living mammals.

A comparison between affiliative and agonistic behaviours in wild and captive Sapajus libidinosus (Spix, 1823) (Mammalia, Primates, Cebidae)

Organisms modulate the expression of their behaviours through environmental contexts. Several studies have suggested that the frequencies of social behaviours may differ between captive and free-living primates. In the present study, we compared the social behaviours displayed by captive and free-living groups of the bearded capuchin monkey (Sapajus libidinosus), describing and analysing their social behaviours. We observed through focal animal sampling 59 animals distributed in 10 social groups, analysing 191:45 h of videos of their behaviours. Captivity reduced the frequency of agonistic, but not of affiliative behaviours. Furthermore, neither group size nor sex could explain the overall variability in affiliative behaviour. We conclude that captivity has indeed an important impact only on some aspects of social behaviour, namely, on agonistic behaviours.(AU)

Group size, temperature and body size modulate the effects of social hierarchy on basal cortisol levels in fishes.

Social rank in a structured society has been linked to basal levels of glucocorticoids in various species, with dominant individuals generally presenting lower levels than subordinates. The biotic and abiotic factors influencing glucocorticoids levels across social ranks are still, however, unclear in fishes. We investigated the influences of group size, fish size, sex, age, and reproduction type, plus water salinity and temperature, on the basal levels of cortisol, the major stress hormone in fishes. A phylogenetically controlled meta-analysis was performed on data from 72 studies over 22 species of fishes. As expected, dominants generally exhibited lower levels of cortisol than subordinates. More importantly, the strength of the correlation between cortisol and rank was modulated by three main factors, group size, environmental temperature, and fish size. Differences in basal cortisol between dominants and subordinates were significantly greater in small groups (dyadic contexts) when compared to larger groups. Differences between dominants and subordinates were also greater in temperate regions when compared to the tropics, and in species with larger body size. These results provide valuable insights into the links among hierarchy, stress and metabolism in fishes.

Psychedelics, Sociality, and Human Evolution.

Our hominin ancestors inevitably encountered and likely ingested psychedelic mushrooms throughout their evolutionary history. This assertion is supported by current understanding of: early hominins' paleodiet and paleoecology; primate phylogeny of mycophagical and self-medicative behaviors; and the biogeography of psilocybin-containing fungi. These lines of evidence indicate mushrooms (including bioactive species) have been a relevant resource since the Pliocene, when hominins intensified exploitation of forest floor foods. Psilocybin and similar psychedelics that primarily target the serotonin 2A receptor subtype stimulate an active coping strategy response that may provide an enhanced capacity for adaptive changes through a flexible and associative mode of cognition. Such psychedelics also alter emotional processing, self-regulation, and social behavior, often having enduring effects on individual and group well-being and sociality. A homeostatic and drug instrumentalization perspective suggests that incidental inclusion of psychedelics in the diet of hominins, and their eventual addition to rituals and institutions of early humans could have conferred selective advantages. Hominin evolution occurred in an ever-changing, and at times quickly changing, environmental landscape and entailed advancement into a socio-cognitive niche, i.e., the development of a socially interdependent lifeway based on reasoning, cooperative communication, and social learning. In this context, psychedelics' effects in enhancing sociality, imagination, eloquence, and suggestibility may have increased adaptability and fitness. We present interdisciplinary evidence for a model of psychedelic instrumentalization focused on four interrelated instrumentalization goals: management of psychological distress and treatment of health problems; enhanced social interaction and interpersonal relations; facilitation of collective ritual and religious activities; and enhanced group decision-making. The socio-cognitive niche was simultaneously a selection pressure and an adaptive response, and was partially constructed by hominins through their activities and their choices. Therefore, the evolutionary scenario put forward suggests that integration of psilocybin into ancient diet, communal practice, and proto-religious activity may have enhanced hominin response to the socio-cognitive niche, while also aiding in its creation. In particular, the interpersonal and prosocial effects of psilocybin may have mediated the expansion of social bonding mechanisms such as laughter, music, storytelling, and religion, imposing a systematic bias on the selective environment that favored selection for prosociality in our lineage.

Recombination mapping of the Brazilian stingless bee Frieseomelitta varia confirms high recombination rates in social hymenoptera.

BACKGROUND: Meiotic recombination is a fundamental genetic process that shuffles allele combinations and promotes accurate segregation of chromosomes. Analyses of the ubiquitous variation of recombination rates within and across species suggest that recombination is evolving adaptively. All studied insects with advanced eusociality have shown exceptionally high recombination rates, which may represent a prominent case of adaptive evolution of recombination. However, our understanding of the relationship between social evolution and recombination rates is incomplete, partly due to lacking empirical data. Here, we present a linkage map of the monandrous, advanced eusocial Brazilian stingless bee, Frieseomelitta varia, providing the first recombination analysis in the diverse Meliponini (Hymenoptera, Apidae). RESULTS: Our linkage map includes 1417 markers in 19 linkage groups. This map spans approximately 2580 centimorgans, and comparisons to the physical genome assembly indicate that it covers more than 75 % of the 275 Megabasepairs (Mbp) F. varia genome. Thus, our study results in a genome-wide recombination rate estimate of 9.3-12.5 centimorgan per Mbp. This value is higher than estimates from nonsocial insects and comparable to other highly social species, although it does not support our prediction that monandry and strong queen-worker caste divergence of F. varia lead to even higher recombination rates than other advanced eusocial species. CONCLUSIONS: Our study expands the association between elevated recombination and sociality in the order Hymenoptera and strengthens the support for the hypothesis that advanced social evolution in hymenopteran insects invariably selects for high genomic recombination rates.

The relationship between pinworm (Trypanoxyuris) infection and gut bacteria in wild black howler monkeys (Alouatta pigra).

Gut bacteria may coexist with other groups of organisms, such as nematode parasites, that inhabit the gastrointestinal tract of primates; however, the possible effects of endoparasites on bacterial communities are frequently overlooked. Here we explored whether infection with Trypanoxyuris, an oxyurid gastrointestinal parasite, is associated with changes in the gut bacterial community of wild black howler monkeys (Alouatta pigra), by comparing gut bacterial communities of consistently infected individuals and individuals that never tested positive for Trypanoxyuris throughout different months across the year. We additionally controlled for other sources of variation reported to influence the primate microbiome including individual identity, social group, and seasonality. Trypanoxyuris infection was not related to differences in gut bacterial alpha diversity, but was weakly associated with differences in gut bacterial community structure. In contrast, among the covariates considered, both individual identity and social group were more strongly associated with variation in the howler gut bacterial community. Our results suggest that gastrointestinal parasites may be associated, to some extent, with shifts in the gut bacterial communities hosted by free-ranging primates, although a causal link still needs to be established. Further studies of wild primate hosts infected with parasite species with different pathogenicity are needed to better elucidate health-related consequences from the parasite-microbiome interplay.

Coexistence of Diversified Dog Socialities and Territorialities in the City of Concepción, Chile.

There has been scant research on the presence of stray dogs in cities. Studying their very considerable presence in Concepción (Chile) provided a unique opportunity to learn more about the different patterns of sociality and territoriality exhibited by the dog species. Via a set of case studies, we examined the behavior of urban dogs, adopting an ethnographic methodology. This yielded findings of the dogs' cognitive, social and spatial adjustment abilities, i.e., their territorialities. Our hypothesis was validated: We found numerous types of sociability, we confirmed the presence of two previously established categories: family dogs (pets, guard dogs and beggars' dogs) and stray dogs (dogs almost entirely unused to humans, aggressive dogs at the far end of the campus and feral dogs in the woods). We also identified three new ones: familiar stray dogs in packs (dogs both spatially and socially close to humans), pet-stray dogs (i.e., village dogs interacting closely with people) and free-roaming pet dogs. We conclude that an ongoing two-way bond between humans and animals allowed these dogs to became part of a city's urban identity and explains the stray dogs' plasticity in terms of adapting to the diversified urban habitat. We postulate that it was the human culture and range of urban areas in Concepción that gave rise to this unique diversity of sociospatial positioning and level of adjustment (e.g., dogs crossing crosswalks).

Do dogs rescue their owners from a stressful situation? A behavioral and physiological assessment.

Rescue behavior is considered a type of pro-social response, defined as a voluntary action directed to benefit another individual who is in a stressful or dangerous situation. In two experiments, we investigated whether dogs would rescue their owners when the person was trapped inside a wooden box and emitted clear signs of stress. The performance of these dogs was compared against that of a control group in which the owners remained calm while trapped. In addition, to assess if training modulated this behavior, we tested a group of dogs from the military trained in search and rescue tasks (Experiment 1). Results showed that dogs opened the box more frequently when the owner pretended to be stressed than when calm. Training shortened latencies to open the door but not the frequency of the behavior. In Experiment 2, we investigated if emotional contagion could be a possible mechanism underlying dogs' rescue responses by measuring dogs' behavior, heart rate, and saliva cortisol level in the stressed and calm conditions, and also controlled for obedience by having the calm owners call their pets while trapped. We replicated the findings of Experiment 1 as more dogs opened the door in the stressed owner condition than in the calm condition. In addition, we observed an increase in heart rate across trials in the stressed condition and a decrease across trials in the calm condition, but no differences in cortisol levels or stress-related behaviors between conditions. In brief, we found evidence that approximately half of the dogs without previous training showed spontaneous rescue behaviors directed to their owners. Neither was this behavior motivated by obedience nor by the motivation to re-establish social contact with the owner. We conclude that emotional contagion is a plausible mechanism underlying dogs' rescue behavior in the present protocol.

Technologies of the Social: Family Constellation Therapy and the Remodeling of Relational Selfhood in China and Mexico.

In this article, we investigate how an increasingly popular therapeutic modality, family constellation therapy (FCT), functions simultaneously as a technology of the self (Foucault, Technologies of the self: a seminar with Michel Foucault, University of Massachusetts Press, Amherst, 1988) as well as what we here call a "technology of the social." In FCT, the self is understood as an assemblage of ancestral relationships that often creates problems in the present day. Healing this multi-generational self involves identifying and correcting hidden family dynamics in high-intensity group sessions where other participants represent the focus client and his/her family members, both alive and deceased. Drawing on ethnographic data collected in multiple FCT workshops in Beijing, China and Oaxaca City, Mexico, we show how FCT ritually reorganizes boundaries between self and other in novel ways, creating a collective space for shared moral reflection on troubling social, historical, and cultural patterns. By demonstrating the ways in which FCT unfolds as both a personal and social technology, this article contributes to ongoing conversations about how to effectively theorize sociality in therapeutic practice, and problematizes critical approaches emphasizing governmentality and commensuration (Mattingly, Moral laboratories family peril and the struggle for a good life, University of California Press, Oakland, 2014; Duncan, Transforming therapy: mental health practice and cultural change in Mexico, Vanderbilt University Press, Nashville, 2018; Matza, Shock therapy: psychology, precarity, and well-being in postsocialist Russia, Duke University Press, Durham, 2018; Pritzker, Presented at "Living Well in China" Conference, Irvine, CA, 2018; Mattingly, Anthropol Theory, 2019; Zigon, "HIV is God's Blessing": rehabilitating morality in neoliberal Russia, University of California Press, Berkeley, 2011).

Small to modest impact of social group on the gut microbiome of wild Costa Rican capuchins in a seasonal forest.

The horizontal transmission of pathogenic and beneficial microbes has implications for health and development of socially living animals. Social group is repeatedly implicated as an important predictor of gut microbiome structure among primates, with individuals in neighboring social groups exhibiting distinct microbiomes. Here we examine whether group membership is a predictor of gut microbiome structure and diversity across three groups of white-faced capuchins (Cebus capucinus imitator) inhabiting a seasonal Costa Rican forest. We collected 62 fecal samples from 18 adult females during four sampling bouts. Sampling bouts spanned the dry-to-wet-to-dry seasonal transitions. To investigate gut microbial composition, we sequenced the V4 region of the 16S rRNA gene. We used the DADA2 pipeline to assign amplicon sequence variants and the RDP database to classify taxa. Our findings are: 1) gut microbiomes of capuchins clustered by social group in the late dry season, but this pattern was less evident in other sampling bouts; 2) social group was a significant variable in a PERMANOVA test of beta diversity, but it accounted for less variation than season; 3) social group was not an important predictor of abundance for the ten most abundant microbial taxa in capuchins; 4) when examining log2-fold abundances of microbes between social groups, there were significant differences in some pairwise comparisons. While this is suggestive of group-wide differences, individual variation may have a strong impact and should be assessed in future studies. Overall, we found a minor impact of social group membership on the gut microbiota of wild white-faced capuchins. Future research including home range overlap and resource use, as well as fine-scale investigation of individual variation, will further elucidate patterns of socially structured microbes.

Genomic Signals of Adaptation towards Mutualism and Sociality in Two Ambrosia Beetle Complexes.

Mutualistic symbiosis and eusociality have developed through gradual evolutionary processes at different times in specific lineages. Like some species of termites and ants, ambrosia beetles have independently evolved a mutualistic nutritional symbiosis with fungi, which has been associated with the evolution of complex social behaviors in some members of this group. We sequenced the transcriptomes of two ambrosia complexes (Euwallacea sp. near fornicatus⁻Fusarium euwallaceae and Xyleborus glabratus⁻Raffaelea lauricola) to find evolutionary signatures associated with mutualism and behavior evolution. We identified signatures of positive selection in genes related to nutrient homeostasis; regulation of gene expression; development and function of the nervous system, which may be involved in diet specialization; behavioral changes; and social evolution in this lineage. Finally, we found convergent changes in evolutionary rates of proteins across lineages with phylogenetically independent origins of sociality and mutualism, suggesting a constrained evolution of conserved genes in social species, and an evolutionary rate acceleration related to changes in selective pressures in mutualistic lineages.