Not feeling the heat? Effects of dietary protein on satiation and satiety in mice are not due to its impact on body temperature.

Dietary protein modulates food intake (FI) via unclear mechanism(s). One possibility is that higher protein leads to greater post-ingestive heat production (Specific dynamic action: SDA) leading to earlier meal termination (increased satiation), and inhibition of further intake (increased satiety). The influence of dietary protein on feeding behaviour in C57BL/6J mice was tested using an automated FI monitoring system (BioDAQ), simultaneous to body temperature (Tb). Total FI, inter meal intervals (IMI, satiety) and meal size (MS, satiation) were related to changes in Tb after consuming low (5%, LP), moderate (15%, MP) and high (30%, HP) protein diets. Diets were tested over three conditions: 1) room temperature (RT, 21 ± 1 °C), 2) room temperature and running wheels (RTRW) and 3) low temperature (10 °C) and running wheels (LTRW). The differences between diets and conditions were also compared using mixed models. Mice housed at RT fed HP diet, reduced total FI compared with LP and MP due to earlier meal termination (satiation effect). FI was lowered in RTRW conditions with no differences between diets. FI significantly increased under LTRW conditions for all diets, with protein content leading to earlier meal termination (satiation) but not the intervals between feeding bouts (satiety). Tb fell immediately after feeding in all conditions. Despite a reduction in total FI in mice fed HP, mediated via increased satiation, this effect was not linked to increased Tb during meals. We conclude effects of dietary protein on intake are not mediated via SDA and Tb.

Atlantic salmon Salmo salar do not prioritize digestion when energetic budgets are constrained by warming and hypoxia.

During summer, farmed Atlantic salmon (Salmo salar) can experience prolonged periods of warming and low aquatic oxygen levels due to climate change. This often results in a drop in feed intake; however, the physiological mechanism behind this behaviour is unclear. Digestion is a metabolically expensive process that can demand a high proportion of an animal's energy budget and might not be sustainable under future warming scenarios. We investigated the effects of elevated temperature and acute hypoxia on specific dynamic action (SDA; the energetic cost of digestion), and how much of the energy budget (i.e. aerobic scope, AS) was occupied by SDA in juvenile Atlantic salmon. AS was 9% lower in 21°C-acclimated fish compared to fish reared at their optimum temperature (15°C) and was reduced by ~50% by acute hypoxia (50% air saturation) at both temperatures. Furthermore, we observed an increase in peak oxygen uptake rate during digestion which occupied ~13% of the AS at 15°C and ~20% of AS at 21°C, and increased the total cost of digestion at 21°C. The minimum oxygen tolerance threshold in digesting fish was ~42% and ~53% at 15 and 21°C, respectively, and when digesting fish were exposed to acute hypoxia, gut transit was delayed. Thus, these stressors result in a greater proportion of the available energy budget being directed away from digestion. Moderate environmental hypoxia under both optimal and high temperatures severely impedes digestion and should be avoided to limit exacerbating temperature effects on fish growth.

Constraints of digestion on swimming performance and stress tolerance vary with habitat in freshwater fish species.

Limited aerobic scope (AS) during digestion might be the main constraint on the performance of bodily functions in water-breathing animals. Thus, investigating the postprandial changes in various physiological functions and determining the existence of a shared common pattern because of possible dependence on residual AS during digestion in freshwater fish species are very important in conservation physiology. All species from slow-flow habitats showed impaired swimming speed while digesting, whereas all species from fast-flow habitats showed strong swimming performance, which was unchanged while digesting. Only two species from slow-flow habitats showed impaired heat tolerance during digestion, suggesting that whether oxygen limitation is involved in the heat tolerance process is species-specific. Three species from slow- or intermediate-flow habitats showed impaired hypoxia tolerance during digestion because feeding metabolism cannot cease completely under hypoxia. Overall, there was no common pattern in postprandial changes in different physiological functions because: (1) the digestion process was suppressed under oxygen-limiting conditions, (2) the residual AS decreased during digestion, and (3) performance was related to residual AS, while digestion was context-dependent and species-specific. However, digestion generally showed a stronger effect on bodily functions in species from slow-flow habitats, whereas it showed no impairment in fishes from fast-flow habitats. Nevertheless, the postprandial change in physiological functions varies with habitat, possibly due to divergent selective pressure on such functions. More importantly, the present study suggests that a precise prediction of how freshwater fish populations will respond to global climate change needs to incorporate data from postprandial fishes.

The Elusive Hypertrophy of the Python Heart.

The Burmese python, one of the world's largest snakes, has reached celebrity status for its dramatic physiological responses associated with digestion of enormous meals. The meals elicit a rapid gain of mass and function of most visceral organs, particularly the small intestine. There is also a manyfold elevation of oxygen consumption that demands the heart to deliver more oxygen. It therefore made intuitive sense when it was reported that the postprandial response entailed a 40% growth of heart mass that could accommodate a rise in stroke volume. Many studies, however, have not been able to reproduce the 40% growth of the heart. We collated published values on postprandial heart mass in pythons, which include several instances of no change in heart mass. On average, the heart mass is only 15% greater. The changes in heart mass did not correlate to the mass gain of the small intestine or peak oxygen consumption. Hemodynamic studies show that the rise in cardiac output does not require increased heart mass but can be fully explained by augmented cardiac filling and postprandial tachycardia. Under the assumption that hypertrophy is a contingent phenomenon, more recent experiments have employed two interventions such as feeding with a concomitant reduction in hematocrit. The results suggest that the postprandial response of the heart can be enhanced, but the 40% hypertrophy of the python heart remains elusive.

The first direct measurements of ventilatory flow and oxygen utilization after exhaustive exercise and voluntary feeding in a teleost fish, Oncorhynchus mykiss.

A new "less invasive" device incorporating an ultrasonic flow probe and a divided chamber, but no stitching of membranes to the fish, was employed to make the first direct measurements of ventilatory flow rate (V̇w) and % O2 utilization (%U) in juvenile rainbow trout (37 g, 8ºC) after exhaustive exercise (10-min chasing) and voluntary feeding (2.72% body mass ration). Under resting conditions, the allometrically scaled V̇w (300 ml kg-1 min-1 for a 37-g trout = 147 ml kg-1 min-1 for a 236-g trout exhibiting the same mass-specific O2 consumption rate, MO2) and the convection requirement for O2 (CR = 4.13 L mmol-1) were considerably lower, and the %U (67%) was considerably higher than in previous studies using surgically attached masks or the Fick principle. After exhaustive exercise, V̇w and MO2 approximately doubled whereas frequency (fr) and %U barely changed, so increased ventilatory stroke volume (Vsv) was the most important contributor to increased MO2. CR declined slightly. Values gradually returned to control conditions after 2-3 h. After voluntary feeding, short-term increases in V̇w, Vsv and MO2 were comparable to those after exercise, and fr again did not change. However, %U increased so CR declined even more. The initial peaks in V̇w, Vsv and MO2, similar to those after exercise, were likely influenced by the excitement and exercise component of voluntary feeding. However, in contrast to post-exercise fish, post-prandial fish exhibited second peaks in these same parameters at 1-3 h after feeding, and %U increased further, surpassing 85%, reflecting the true "specific dynamic action" response. We conclude that respiration in trout is much more efficient than previously believed.

The physiological consequences of a very large natural meal in a voracious marine fish, the staghorn sculpin (Leptocottus armatus).

Little information exists on physiological consequences when wild fish eat natural food. Staghorn sculpins at 10-13°C voluntarily consumed 15.8% of their body mass in anchovies. Gastric clearance was slow with >60% of the meal retained in the stomach at 48 h, and was not complete until 84 h. At 14-24 h post-feeding, pH was depressed by 3 units and Cl- concentration was elevated 2-fold in gastric chyme, reflecting HCl secretion, while in all sections of the intestine, pH declined by 1 pH unit but Cl- concentration remained unchanged. PCO2 and total ammonia concentration were greatly elevated throughout the tract, whereas PNH3 and HCO3- concentration were depressed. Intestinal HCO3- secretion rates, measured in gut sacs in vitro, were also lower in fed fish. Whole-animal O2 consumption rate was elevated approximately 2-fold for 72 h post-feeding, reflecting 'specific dynamic action', whereas ammonia and urea-N excretion rates were elevated about 5-fold. Arterial blood exhibited a modest 'alkaline tide' for about 48 h, but there was negligible excretion of metabolic base to the external seawater. PaCO2 and PaO2 remained unchanged. Plasma total amino acid concentration and total lipid concentration were elevated about 1.5-fold for at least 48 h, whereas small increases in plasma total ammonia concentration, PNH3 and urea-N concentration were quickly attenuated. Plasma glucose concentration remained unchanged. We conclude that despite the very large meal, slow processing with high efficiency minimizes internal physiological disturbances. This differs greatly from the picture provided by previous studies on aquacultured species using synthetic diets and/or force-feeding. Questions remain about the role of the gastro-intestinal microbiome in nitrogen and acid-base metabolism.

Dietary carbohydrates induce a higher SDA than lipids in rainbow trout (Oncorhynchus mykiss) based on environmental temperature.

As global temperatures increase so do the needs to investigate how the energy metabolism of fish responds to a broad range of thermal condition. Limited resources make it additionally important to use them sustainably in the feeds for aquaculture. Here we investigated the use of three different carbohydrate to lipid ratios (1:1; 1: 0.6; 1.4: 1 as non-protein energy substrates (NPES) in diets for rainbow trout (Oncorhynchus mykiss) under five different thermal regimes (12; 14; 16; 18; 20 °C) in a bioenergetic approach using a group respirometer. The results showed that the diet with carbohydrate as the main NPES resulted in a quadratic relationship of the specific dynamic action (SDA) values to temperature while diets with lipid as main NPES or a balanced ratio did not show such a response. SDA values in the diet with carbohydrate as the main NPES were significantly higher at temperatures around the optimum (15-17 °C) than the diets with lipid as the main NPES or with a balanced carbohydrate to lipid ratio. The retained energy (RE) was highly dependent on the standard metabolic rate (SMR) and SDA values did not carry over onto them. The protein utilization for energy combustion was significantly lower at 12 °C in the diet with carbohydrate as the main NPES than in the diet with lipid as the main NPES thus indicating that carbohydrates hold a relevant nutritional value especially at lower temperatures.

The Role of Metabolic Phenotype in the Capacity to Balance Competing Energetic Demands.

AbstractGiven the critical role of metabolism in the life history of all organisms, there is particular interest in understanding the relationship between individual metabolic phenotypes and the capacity to partition energy into competing life history traits. Such relationships could be predictive of individual phenotypic performances throughout life. Here, we were specifically interested in whether an individual fish's metabolic phenotype can shape its propensity to feed following a significant stressor (2-min exhaustive exercise challenge). Such a relationship would provide insight into previous intraspecific observations linking high metabolism with faster growth. Using a teleost fish, the barramundi (Lates calcarifer), we predicted that individuals with high standard metabolic rates (SMRs) and maximal metabolic rates (MMRs) would be faster to recover and resume feeding after exercise. Contrary to our prediction, neither SMR nor MMR was correlated with latency to feed after exercise (food was offered at 0.5, 1.5, 3, and 18 h after exercise). Only time after exercise and individual fish ID were significant predictors of latency to feed. Measurements of MMR from the same individuals (three measurements spaced 8-12 d apart) revealed a moderate degree of repeatability (R=0.319). We propose that interindividual differences in biochemical and endocrine processes may be more influential than whole-organism metabolic phenotype in mediating feeding latency after exercise.

The relationships between specific dynamic action, nutrient retention and feed conversion ratio in farmed freshwater Chinook salmon (Oncorhynchus tshawytscha).

Improving the feed conversion ratio (FCR; the amount of feed consumed relative to the amount of weight gain) can reduce both production costs and environmental impacts of farmed fish. The aim of this study was to investigate what drives FCR to understand how nutrients are retained, as well as the amount of oxygen consumed for digestion, absorption and assimilation (a metabolic process known as specific dynamic action, SDA). Feed-efficient and inefficient Chinook salmon (Oncorhynchus tshawytscha) in fresh water were identified using ballotini beads and X-radiography that tracked individual feed intake across three assessment periods under satiated feeding. This allowed a comparison of physiological traits and body composition between the two FCR phenotypes over two time points as Chinook salmon grew from 305 to 620 g. Fish with higher daily feed intake (DFI) had higher daily weight gain (DWG) as expected. Nonetheless, the relationship between FCR and DFI as well as FCR and DWG was variable between time points. FCR and DWG were not correlated at the first time point and were negatively correlated at the second time point. In contrast, FCR and DFI were positively correlated at the first time point but not the second. Despite this, efficient fish ate smaller meals and retained more protein, lipid and energy in their body tissues. There was no detectable difference in metabolism between the two FCR phenotypes with respect to minimal resting metabolic rate, maximum metabolic rate, aerobic scope, or SDA parameters. In conclusion, FCR is not consistently associated with growth and metabolic differences in freshwater Chinook salmon, but FCR-efficient fish retain more nutrients and consume smaller meals.

Interactive effects of food deprivation state and hypoxia on the respiratory responses of postprandial rock crabs, Cancer irroratus.

Under the background of climate change, increasing attention has focused on the effects of ocean deoxygenation on marine organisms. However, few studies address the effects of different food deprivation states on hypoxia tolerance. We therefore investigated the metabolic responses of the Atlantic rock crab, Cancer irroratus (starved 28-35 days, fasted 3-5 days and recently fed). Starved-crab exhibited the lowest critical oxygen saturation (Scrit), while fed-crab had the highest Scrit. The fed-crab maintained an elevated postprandial oxygen consumption (MO2) even below the Scrit of fasted-crab indicating reserved aerobic scopes for critical activities in severe hypoxia. Following feeding, hypoxia (50% and 20% oxygen saturation, SO2) retarded the specific dynamic action resulting in lower peak MO2 and longer duration. The starved-crab exhibited a lower peak MO2, prolonged duration and higher energy expenditure than fasted-crab after feeding. The decline in arterial PO2 was most pronounced below the Scrit for both fasted- and starved-crab. The higher hemocyanin concentration ([Hc]) of fasted-crab (than starved-crab) suggested they had improved oxygen transport capacity, but hypoxia did not increase [Hc] during the 72-h experiment. Following feeding, the fasted-crab significantly increased L-lactate concentration ([L-lactate]) in 20% SO2, which was not observed in starved-crab. These results suggest starvation may trigger a cross-tolerance to hypoxia. Because crabs can undergo long periods of food deprivation in their natural environment, future studies should consider how this may affect their ability to deal with environmental perturbations.

Divergence in digestive and metabolic strategies matches habitat differentiation in juvenile salmonids.

Divergent energy acquisition and processing strategies associated with using different microhabitats may allow phenotypes to specialize and coexist at small spatial scales. To understand how ecological specialization affects differentiation in energy acquisition and processing strategies, we examined relationships among digestive physiology, growth, and energetics by performing captive experiments on juveniles of wild coho salmon (Oncorhynchus kisutch) and steelhead trout (O. mykiss) that exploit adjacent habitats along natural low-to-high energy flux gradients (i.e., pools versus riffles) in coastal streams. We predicted that: (i) the specialization of steelhead trout to high-velocity, high-energy habitats would result in elevated food intake and growth at the cost of lower growth efficiency relative to coho salmon; (ii) the two species would differentiate along a rate-maximizing (steelhead trout) versus efficiency-maximizing (coho salmon) axis of digestive strategies matching their ecological lifestyle; and (iii) the higher postprandial metabolic demand (i.e., specific dynamic action, SDA) associated with elevated food intake would occupy a greater fraction of the steelhead trout aerobic budget. Relative to coho salmon, steelhead trout presented a pattern of faster growth and higher food intake but lower growth efficiency, supporting the existence of a major growth versus growth efficiency trade-off between species. After accounting for differences in ration size between species, steelhead trout also presented higher SDA than coho salmon, but similar intestinal transit time and lower assimilation efficiency. Both species presented similar aerobic budgets since the elevated SDA of steelhead trout was largely compensated by their higher aerobic scope relative to coho salmon. Our results illustrate the key contribution of digestive physiology to the adaptive differentiation of juvenile growth, energetics, and overall performance of taxa with divergent habitat specializations along a natural productivity gradient.

Feeding effects on liver mitochondrial bioenergetics of Boa constrictor (Serpentes: Boidae).

Snakes are interesting examples of taxa that can overcome energy metabolism challenges, as many species can endure long periods without feeding, and their eventual meals are of reasonably large sizes, thus exhibiting dual extreme adaptations. Consequently, metabolic rate increases considerably to attend to the energetic demand of digestion, absorption and protein synthesis. These animals should be adapted to transition from these two opposite states of energy fairly quickly, and therefore we investigated mitochondrial function plasticity in these states. Herein, we compared liver mitochondrial bioenergetics of the boid snake Boa constrictor during fasting and after meal intake. We fasted the snakes for 60 days, and then we fed a subgroup with 30% of their body size and evaluated their maximum postprandial response. We measured liver respiration rates from permeabilized tissue and isolated mitochondria. From isolated mitochondria, we also measured Ca2+ retention capacity and redox status. Mitochondrial respiration rates were maximized after feeding, reaching an approximately 60% increase from fasting levels when energized with complex I-linked substrates. Interestingly, fasting and fed snakes exhibited similar respiratory control ratios and citrate synthase activity. Furthermore, we found no differences in Ca2+ retention capacity, indicating no increase in susceptibility to mitochondrial permeability transition, and no changes in mitochondrial redox state, although fed animals exhibited increases in the release of H2O2. Thus, we conclude that liver mitochondria from B. constrictor snakes increase respiration rates during the postprandial period and quickly improve the bioenergetic capacity without compromising redox balance.

Effects of change in temperature on the cardiac contractility of broad-snouted caiman (Caiman latirostris) during digestion.

In many reptiles, digestion has been associated with the selection of higher body temperatures, the so-called post-prandial thermophilic response. This study aimed to investigate the excitation-contraction (E-C) coupling in postprandial broad-snouted caimans (Caiman latirostris) in response to acute warming within a preferred body temperature range of crocodiles. Isometric preparations subjected to a temperature transition from 25°C to 30°C were used to investigate myocardial contractility of postprandial caimans, that is, 48 h after the animals ingested a rodent meal corresponding to 15% of body mass. The caiman heart exhibits a negative force-frequency relationship that is independent of the temperature. At 25°C, cardiac muscle was able to maintain a constant force up to 36 bpm, above which it decreased significantly, reaching minimum values at the highest frequency of 84 bpm. Moreover, E-C coupling is predominantly dependent on transsarcolemmal Ca2+ transport denoted by the lack of significant ryanodine effects on force generation. On the contrary, ventricular strips at 30°C were able to sustain the cardiac contractility at higher pacing frequencies (from 12 to 144 bpm) due to an important role of Na+ /Ca2+ exchanger in Ca2+ cycling, as indicated by the decay of the post-rest contraction, and a significant contribution of the sarcoplasmic reticulum above 72 bpm. Our results demonstrated that the myocardium of postprandial caimans exhibits a significant degree of thermal plasticity of E-C coupling during acute warming. Therefore, myocardial contractility can be maximized when postprandial broad-snouted caimans select higher body temperatures (preferred temperature zone) following feeding.

The physiological response to digestion in snakes: A feast for the integrative physiologist.

Many snakes can subdue and swallow very large prey after many months of fasting. The functional capacity and the mass of the gastrointestinal organs regress during fasting, but are quickly restored upon feeding. This phenotypic flexibility appears to be energetically inexpensive, and represents a key adaptation that enables snakes to match digestive performance without compromising bodily energy stores prior to nutrient absorption. The reorganization of the intestines resembles the unfolding of an accordion where the individual enterocytes expand, primarily in response to luminal presence of nutrients. The very large rise in postprandial metabolism (specific dynamic action), where the rate of oxygen consumption can increase four- to six-fold, is likely due to a global rise in protein synthesis in all tissues. The rise in oxygen consumption is sustained by a pronounced tachycardia that, in part, is caused by un-identified humoral factor(s) with positive chronotropic effects, and a rise in stroke volume, where venous return may be augmented by a rise in venous tone. The immediate stimulation of gastric acid secretion causes a metabolic alkalosis (the alkaline tide), but pH remains unchanged due to a rise in arterial PCO2 caused by a proportionally smaller elevation of ventilation than for CO2 production (i.e., hypoventilation). Given the magnitude of the physiological responses to feast and famine, snakes provide a unique animal model to study regulation of organ function in response to rapid transitions in demands as well as an avenue to study a multitude of functional interactions among organ systems.

Postprandial cardiorespiratory responses and the regulation of digestion-associated tachycardia in Nile tilapia (Oreochromis niloticus).

Cardiorespiratory adjustments that occur after feeding are essential to supply the demands of digestion in vertebrates. The well-documented postprandial tachycardia is triggered by an increase in adrenergic activity and by non-adrenergic non-cholinergic (NANC) factors in mammals and crocodilians, while it is linked to a withdrawal of vagal drive and NANC factors in non-crocodilian ectotherms-except for fish, in which the sole investigation available indicated no participation of NANC factors. On the other hand, postprandial ventilatory adjustments vary widely among air-breathing vertebrates, with different species exhibiting hyperventilation, hypoventilation, or even no changes at all. Regarding fish, which live in an environment with low oxygen capacitance that requires great ventilatory effort for oxygen uptake, data on the ventilatory consequences of feeding are also scarce. Thus, the present study sought to investigate the postprandial cardiorespiratory adjustments and the mediation of digestion-associated tachycardia in the unimodal water-breathing teleost Oreochromis niloticus. Heart rate (fH), cardiac autonomic tones, ventilation rate (fV), ventilation amplitude, total ventilation and fH/fV variability were assessed both in fasting and digesting animals under untreated condition, as well as after muscarinic cholinergic blockade with atropine and double autonomic blockade with atropine and propranolol. The results revealed that digestion was associated with marked tachycardia in O. niloticus, determined by a reduction in cardiac parasympathetic activity and by circulating NANC factors-the first time such positive chronotropes were detected in digesting fish. Unexpectedly, postprandial ventilatory alterations were not observed, although digestion triggered mechanisms that were presumed to increase oxygen uptake, such as cardiorespiratory synchrony.

Do prolonged fasting periods influence the postprandial metabolic responses in turtles? What can Trachemys scripta elegans teach us about this?

The postprandial period is characterized by a modification of the gastrointestinal activity after food intake, accompanied by an increase in metabolic rate, secretion of acids, and absorption of nutrients. For ectothermic vertebrates, those changes are particularly prominent given the relatively low metabolic cost and the low frequency of food uptake. However, prolonged fasting periods decrease energy reserves and may compromise the upregulation of costly processes, such as the increase in metabolic rate after resuming the meal intake. Assuming that the main source of energy needed to support such events is provided from the animal's own body reserves, our aim with this study is to test the hypothesis that the longer the period of fasting, the smaller the metabolic rate increase during the postprandial period, since lesser energy reserves trigger these increases. For this, we measured the oxygen consumption rates (V̇O2 ) of red-eared slider turtles, Trachemys scripta elegans, submitted to different periods of fasting (47 and 102 days), before and after the ingestion of meals equivalent to 5% of their body masses. Despite the longer fasting period, which led to a reduction of 10.77% in the body mass of the turtles, there were no differences between the two experimental groups regarding maximum V̇O2 values after food intake (V̇O2 peak), postprandial metabolic scope, mean time to V̇O2 peak, and postprandial duration. Results indicate that 102 fasting days does not compromise aerobic metabolic increase during postprandial period and does not impair digestive process of the turtles, even with a loss of body mass.

Metabolic physiology of the freshwater planaria Girardia dorotocephela and Schmidtea mediterranea: reproductive mode, specific dynamic action, and temperature.

Planarians are widely used animal models for studies in regeneration, developmental biology, neurobiology, and behavior. However, surprisingly little is known about other aspects of their basic biology, even though such information might help validate these flatworms as a general animal model. We hypothesized that planaria, although dependent on simple diffusion of O2 across the integument for O2 uptake, would nonetheless show changes in oxygen consumption (V̇o2) associated with reproductive mode (sexual or asexual), feeding (specific dynamic action; SDA), temperature (Q10 values), and photoperiod typical of those responses of more complex invertebrates. In the current experiments, routine V̇o2 was measured over the range of 13-28°C in Schmidtea mediterranea and Girardia dorotocephala. At the long-term maintenance temperature of 18°C, routine V̇o2 was ~13 µL O2·g-1·h-1 in the two asexual strains, but approximately twice as high (27 µL O2·g-1·h-1) in the sexual strain of S. mediterranea, suggesting a metabolic cost for sexual reproduction. Metabolic temperature sensitivity, measured by Q10, was about one to three for all three groups. All three groups showed a large (~2- to 3-fold) increase in V̇o2 within a day following feeding, suggesting a large SDA effect. Starvation, causing "degrowth" in some planaria, resulted in a loss of one-third of body mass in sexual S. mediterranea but no body mass loss in either asexual strains. Collectively, these data indicate that, while being a relatively simple flatworm with no dedicated respiratory or circulatory system, their metabolic physiological responses are quite similar to those shown by more complex invertebrates and vertebrates, contributing to their validation as an animal model.

Modest Regulation of Digestive Performance Is Maintained through Early Ontogeny for the American Alligator, Alligator mississippiensis.

The American alligator, Alligator mississippiensis, is an opportunistic carnivore that experiences an ontogenetic shift in food and feeding habits with an increase in body size. Alligators frequently feed on invertebrates and small fish as neonates and transition to feeding less frequently on larger vertebrates as they grow. We hypothesized that alligators experience an ontogenetic shift in the regulation of intestinal performance-modest regulation with frequent feeding early in life and wider regulation with less frequent feeding as they increase in body size. We tested this hypothesis by comparing postprandial responses in metabolic rate, organ masses, intestinal histology, digestive hydrolase activities, and intestinal nutrient uptake rates among neonate, juvenile, and subadult alligators. With feeding, alligators of all three age classes experienced a rapid increase in metabolic rate that peaked within 2 d and thereafter declined more slowly to prefeeding rates. Specific dynamic action increased with body mass and was equivalent to 32% of meal energy. For each age class, the majority of organs did not change in wet and dry mass with feeding. For subadult alligators, luminal gut pH varied regionally due to the acidic stomach, which continued to remain acidic with fasting. With feeding, epithelial enterocytes are remodeled from a pseudostratified to a stratified architecture and become infiltrated with lipid droplets. Feeding did not generate any significant change in the thickness of intestinal tissues, though it did induce an increase in enterocyte width and volume for subadults. For each age class, feeding generally did not result in significant changes in pancreatic trypsin, intestinal aminopeptidase, and intestinal nutrient uptake activities and capacities. Mass-specific nutrient uptake rates varied among age classes due to the higher rates exhibited by neonates. Among age classes, intestinal uptake capacities scaled allometrically (mass exponents <1) with body mass. Across these three age classes, the modest regulation of digestive performance with feeding and fasting for alligators appears to be ontogenetically conserved.

The effect of temperature and meal size on the aerobic scope and specific dynamic action of two temperate New Zealand finfish Chrysophrys auratus and Aldrichetta forsteri.

Shallow coastal and estuarine habitats function as nurseries for many juvenile fish. In this comparative study, metabolic profiles of two New Zealand finfish, snapper (Chrysophrys auratus) and yellow-eyed mullet-YEM (Aldrichetta forsteri) that as juveniles share the same temperate coastal environments, were examined. Metabolic parameters (routine and maximum metabolic rates, and specific dynamic action-SDA) were investigated at a set of temperatures (13, 17, 21 °C) within the range juveniles both species experience annually. SDA was also determined for a range of different feed rations to investigate the effects of meal size on postprandial metabolic response. Temperature was a strong modulator of snapper and YEM metabolic profile (routine and maximum metabolic rates, and absolute and factorial aerobic scope). Metabolic rates increased with temperature in both species as did absolute scope in YEM, though for snapper, it was only greater at the highest temperature. Factorial scope behaved in the same fashion for the two species, being greatest at 13 °C. Both absolute and factorial scope were ~ twofold greater in YEM than in snapper across the entire temperature range. Temperature also affected SDA response in snapper, while in YEM, SDA parameters were largely unaffected when temperature increased from 17 to 21 °C. Snapper were able to consume a large range of meal sizes (0.5-3.0% body mass-BM) with meal sizes > 1% BM having a pronounced effect on numerous SDA parameters, whereas mullet appeared to consume more limited ration sizes (≤ 1.0% BM). In both species, rations ≤ 1% BM produced similar changes in SDA parameters identifying comparable digestive bio-energetics. Overall, our metabolic characterisations demonstrate that both species can adjust to the variable temperate environmental temperatures and manage the energetic costs of digestion and feed assimilation. Yet, despite these general similarities, YEM's greater aerobic scope may point to better physiological adaptation to the highly variable temperate coastal environment than were observed in snapper.

Does global warming contribute to the obesity epidemic?

Endotherms must expend more energy to digest colder food, so they acquire more calories by eating food at a higher temperature than eating the identical food cold. A recent study shows that ownership of a microwave is associated with a small increase in BMI and obesity. The same logic applies to other substances that endotherms introduce into their bodies, like air. An analysis of the National Longitudinal Study of Adolescent to Adult Health (Add Health) shows that, net of sex, age, race, education, earnings, neighborhood characteristics, and exercise activities, atmospheric temperature is associated with small but statistically significant increases in BMI, weight, overweight, and obesity. Atmospheric temperature is more strongly associated than most exercise activities, and as strongly associated as age and population density. An average American might reduce weight by 15.1 lbs, BMI by 2.52 (half the difference between normal weight and obesity), and the odds of obesity by 54% by moving from Phoenix, AZ, to Barrow, AK, or, less dramatically, 5.7 lbs in weight, .95 in BMI (a fifth of the difference between normal weight and obesity), and 25% in the odds of obesity by moving mere 150 miles north to Flagstaff, AZ. Global warming under the worst-case scenario might produce an increase of 2.2 lbs in weight, .37 in BMI, and 12% in odds of obesity from 1961 to 2081.