Regulating the dynamic wetting behavior of pesticide on banana leaves at different growth stages with surfactants.

BACKGROUND: Pesticide spraying constitutes an essential component of the production and management regimen within banana orchards, extending throughout the entire growth cycle of the banana plants. Exploring the intricate interplay between surfactants, pesticide formulations, and the evolving surface properties of banana leaves throughout their growth stages is critical to the enhancement of pesticide application methods and the elevation of agricultural productivity. RESULTS: Through investigating the regulatory impact of surfactants on the physicochemical properties of medicinal solutions, this study elucidates the interaction mechanism between the physicochemical properties of pesticides and the surface characteristics of banana leaves. The findings reveal that the energy dissipation rate of pesticide droplets exhibits a natural exponential rise in correlation with the increase in both the We number and the concentration of surfactant present. Comparatively, the adaxial surface of banana leaves demonstrates superior spreading and adhesion properties for droplets than the abaxial surface. Specifically, droplets containing the anionic surfactant sodium dodecyl sulfate on the adaxial surface of banana leaves are found to spread well with a reduced retraction effect. Conversely, the application of the non-ionic surfactant fatty acid polyoxyethylene ether (AEO-3) on the abaxial surface of banana leaves is more beneficial for the wetting and retention of droplets. As banana leaves grow, there is a noted decline in the spreading and retraction properties of droplets. However, droplets have a higher propensity to wet and adhere to the surfaces of mature banana leaves. CONCLUSION: To bolster the adherence of pesticide droplets to leaf surfaces, it is imperative to ensure they possess superior spreading properties and a controlled retraction pace. This facilitates an extended period of contact and enhanced stability, thereby optimizing the spray's deposition efficacy. © 2024 Society of Chemical Industry.

Resonant oscillation of droplets under an alternating electric field to enhance solute diffusion.

This study investigates a novel microfluidic mixing technique that uses the resonant oscillation of coalescent droplets. During the vertical contact-separation process, solutes are initially separated as a result of the combined effects of diffusion and gravity. We show that the application of alternating current (AC) voltage to microelectrodes below the droplets causes a resonant oscillation, which enhances the even distribution of the solute. The difference in concentration between the top and bottom droplets exhibits frequency dependence and indicates the existence of a particular AC frequency that results in a homogeneous concentration. This frequency corresponds to the resonance frequency of the droplet oscillation that is determined using particle tracking velocimetry. To understand the mixing process, a phenomenological model based on the equilibrium between surface tension, viscosity, and electrostatic force was developed. This model accurately predicted the resonance frequency of droplet flow and was consistent with the experimental results. These results suggest that the resonant oscillation of droplets driven by AC voltage significantly enhances the diffusion of solutes, which is an effective approach to microfluid mixing.

Wetting characterisation on complex surfaces by an automatic open-source tool: DropenVideo.

HYPOTHESIS: Investigating solid-liquid interactions to determine advancing and receding contact angles, and consequently contact angle hysteresis, is crucial for understanding material wetting properties. A reliable, automated, and possibly open-source tool is desirable, to standardize and automatize the measurement and make it user-independent. EXPERIMENTS: This study introduces an open-source software, DropenVideo, as an extension of Dropen. DropenVideo automates frame-by-frame video analysis for the advancing and receding contact angle determination, by considering needle presence, contrast tuning, and compensating for missing drop edge data. Contact angles are calculated using convolution mask, circle, and polynomial fittings. An innovative feature in DropenVideo is the automatic protocol for identifying advancing and receding contact angles: (i) the advancing contact angle is determined as the average value during drop inflation; and (ii) the receding contact angle is determined from the frame of incipient motion during drop deflation. FINDINGS: Exploring the application of DropenVideo across a range of complex surfaces as representative test cases, we highlight existing challenges in interpreting wetting measurements by addressing different wetting scenarios. Our study demonstrates that employing frame-by-frame automatic analysis of contact angle measurement videos using DropenVideo significantly mitigates the potential risks of subjective bias associated with manual interpretation and enhances the precision of identified wetting characteristics.

The Effect of Water Dousing on Heat Strain and Performance During Endurance Running in the Heat.

OBJECTIVES: Assess the effect of water dousing on heat strain and performance during self- and fixed-paced exercise in the heat. DESIGN: Crossover, block-randomized controlled trial. METHODS: Thirteen trained runners completed a 10-km time trial (TT) and 60-minute fixed-pace run (60% velocity of V˙O2max) in a 30.4 °C, 47.4% relative humidity environment using either water dousing (DOUSE) or no dousing (CON). RESULTS: Ten-kilometer TT performance was faster in DOUSE compared to CON (44:11 [40:48, 47:34] vs 44:38 [41:21, 47:56] min:s; P = .033). Change in core temperature (Tc) was not different between groups during the TT (+0.02 [-0.04, 0.07] °C in DOUSE; P = .853) or fixed-pace run (+0.02 [-0.15, 0.18] °C; P = .848). Change in mean skin temperature was lower in DOUSE during the TT (-1.80 [-2.15, -1.46] °C; P < .001) and fixed-pace run (-1.38 [-1.81, -0.96] °C; P < .001). Heart rate was lower for DOUSE during the fixed-pace run (-3.5 [-6.8, -0.2] beats/min; P = .041) but not during the TT (-0.2 [-2.5, 2.1] beats/min; P = .853). Thermal sensation was lower for DOUSE during the TT (-49.3 [-72.1, -26.1] mm; P < .001) and fixed-pace run (-44.7 [-59.7, -29.6] mm; P < .001). Rating of perceived exertion was not different between groups for the TT (-0.2 [-0.7, 0.3]; P = .390) or fixed-pace run (-0.2 [-0.8, 0.4]; P = .480). Sweat rate was lower for DOUSE for the TT (-0.37 [-0.53, -0.22] L/h; P < .001) and fixed-pace run (-0.37 [-0.48, -0.26] L/h; P < .001). CONCLUSION: Water dousing improves 10-km TT performance in the heat but does not affect Tc. The positive change in thermal perception (via lower skin temperature) during the TT likely drives this benefit.

Estimation of the Structure of Hydrophobic Surfaces Using the Cassie-Baxter Equation.

The effect of extreme water repellency, called the lotus effect, is caused by the formation of a Cassie-Baxter state in which only a small portion of the wetting liquid droplet is in contact with the surface. The rest of the bottom of the droplet is in contact with air pockets. Instrumental methods are often used to determine the textural features that cause this effect-scanning electron and atomic force microscopies, profilometry, etc. However, this result provides only an accurate texture model, not the actual information about the part of the surface that is wetted by the liquid. Here, we show a practical method for estimating the surface fraction of texture that has contact with liquid in a Cassie-Baxter wetting state. The method is performed using a set of ethanol-water mixtures to determine the contact angle of the textured and chemically equivalent flat surfaces of AlSI 304 steel, 7500 aluminum, and siloxane elastomer. We showed that the system of Cassie-Baxter equations can be solved graphically by the wetting diagrams introduced in this paper, returning a value for the texture surface fraction in contact with a liquid. We anticipate that the demonstrated method will be useful for a direct evaluation of the ability of textures to repel liquids, particularly superhydrophobic and superoleophobic materials, slippery liquid-infused porous surfaces, etc.

A Raman measurement scheme for accurate compositional analysis of dark-colored sample by minimizing laser-induced sample degradation: Determination of Fe3O4 concentration in sintered ores.

An effective and rapid Raman measurement scheme to determine Fe3O4 concentration in sintered ores was explored. Because sintered ores are brownish-black materials that easily absorb laser photons, accurate quantitative analysis requires obtaining an Fe3O4 peak with a high signal-to-ratio by reducing the possibility of local sample heating and degradation. For this purpose, a wide area coverage (WAC) Raman scheme with a laser-illumination diameter of 1 mm was adopted to decrease the laser power per area (LP/A) on each sample. The sintered ore sample was also wetted with water to reduce the chance of further heating by the laser. The combination of the WAC scheme and water-wetting allowed to increase the laser power during sample measurement, and the subsequent intensity (as well as the signal-to-noise ratio) of the Fe3O4 peak was elevated compared with both that measured by a Raman microscope yielding a higher LP/A and without water-wetting of the sample. In the Raman spectra of 93 real sintered ore samples measured using the proposed scheme, the ratio of Fe3O4 and Fe2O3 peak areas correlated closely (R2 = 0.94) with Fe3O4 concentration determined by titration. The demonstrated scheme is practical when Raman spectroscopy is employed for compositional analysis of dark and highly photon-absorbing samples.

The synaptonemal complex assembles between meiotic chromosomes by wetting.

Exchange of genetic information between the parental chromosomes during sexual reproduction is controlled by a conserved structure called the synaptonemal complex. It is composed of axes (stiff chromosomal backbones), and a central region that assembles between two parallel axes. To form exchanges, the parental chromosomes must be drawn together and aligned by the synaptonemal complex. However, its mechanism of assembly remains unknown. Here we identify an axis-central region interface in C. elegans composed of the axis component HIM-3 and the central region component SYP-5. Weaker interface prevented complete synaptonemal complex assembly, and crucially, altered its canonical layered ultrastructure. Informed by these phenotypes, we built a thermodynamic model for synaptonemal complex assembly. The model recapitulates our experimental observations, indicating that the liquid-like central region can move chromosomes by wetting the axes without active energy consumption. More broadly, our data show that condensation can bring about tightly regulated nuclear reorganization.

Impact of different water-reducing agents on the properties of limonite self-compacting conductive concrete.

The application environment for concrete is becoming increasingly complex, accompanied by an intensification of its functional requirements. This paper presents a method for developing self-compacting concrete with conductive properties using limonite and graphite as the concrete conductive phases. In the process of concrete preparation, the limonite is initially treated by a pre-wetting method to prevent the surface depression caused by the addition of limonite during the concrete curing process. The second stage of the process involved optimising different proportions of limonite and graphite and different dosages of water-reducing agent, defoamer and dispersant to prepare concrete. The influence of different dosages of limonite and graphite and different dosages of water-reducing agent on the mechanics and electrical conductivity of concrete was studied in order to obtain self-compacting conductive concrete with performance indicators meeting the requirements of self-compacting and electrical conductivity. The results demonstrate that the mechanical and electrical properties of self-compacting conductive concrete prepared with polycarboxylic acid superplasticizer and retarding superplasticizer combined with superplasticizer are satisfactory, and the composite superplasticizer can function in conjunction with dispersant. The self-compaction index, slump expansion, expansion time T50 and J-ring expansion of fluid concrete meet the requisite standards. Once the concrete has reached the designated curing age, its compressive strength and flexural strength align with the anticipated design expectations, while its resistivity meets the stipulated conductivity index requirements.

Surface energy-induced anti-wetting and anti-fouling enhancement of Janus membrane for membrane distillation.

Membrane distillation (MD) presents a promising alternative to conventional desalination systems, particularly for the treatment of hypersaline wastewater. However, the large-scale application of MD is hindered by challenges such as membrane wetting, membrane fouling, and low permeate flux. Herein, we proposed an air/liquid interface deposition method to fabricate a Janus membrane, termed the PVDF-PDA/PEI-Si membrane. The membrane featured a nanosieving, superhydrophilic polydopamine/polyethylenimine (PDA/PEI) layer decorated with silica nanoparticles, coupled with a microporous, hydrophobic polyvinylidene fluoride (PVDF) layer. The introduction of a dense PDA/PEI-Si layer featuring high surface energy significantly enhanced the wetting and fouling resistance of the membrane, with a minor effect on the permeate flux. The performance enhancement was particularly evident when hypersaline water containing sodium dodecyl sulfate (SDS) and oily contaminants was used as the feed. The interactions between the membrane and contaminants were calculated using the XDLVO theory and molecular dynamics simulations to elucidate the mechanisms underlying the enhanced anti-wetting and anti-fouling properties, respectively. According to the XDLVO theory, a large energy barrier must be overcome for the SDS to attach onto the PDA/PEI-Si surface. Meanwhile, molecular dynamics simulations confirmed the weak interaction energy between the oily foulants and the PVDF-PDA/PEI-Si membrane due to its high surface energy. This study presents a promising approach for the fabrication of high-performance MD membranes and provides new insights into the mechanisms underlying the enhanced anti-wetting and anti-fouling properties.

A two-stage mechanistic reduced-order model of pharmaceutical tablet dissolution: Population balance modeling and tablet wetting functions.

We propose a two-stage reduced-order model (ROM) of pharmaceutical tablet dissolution that is comprised of (i) a mechanistic dissolution function of the active pharmaceutical ingredient (API) and (ii) a tablet wetting function. The former is derived from a population balance model, using a high-resolution finite volume algorithm for a given API crystal size distribution and dissolution rate coefficient. The latter is obtained from the mechanistic understanding of water penetration inside a porous tablet, and it estimates the rate at which the API is exposed to the buffer solution for a given formulation and the dimensions of the tablet, contact angle, and surface tension between the solid and liquid phases, liquid viscosity, and mean effective capillary radius of the pore solid structure. In turn, the two-stage model is mechanistic in nature and one-way coupled by means of convolution in time to capture the start time of the API dissolution process as water uptake, swelling, and disintegration take place. The two-stage model correlates dissolution profiles with critical process parameters (CPPs), critical material attributes (CMAs), and other crucial critical quality attributes (CQAs). We demonstrate the model's versatility and effectiveness in predicting the dissolution profiles of diverse pharmaceutical formulations. Specifically, we formulate and fabricate acetaminophen and lomustine solid tablets using different API content and size distributions, characterize their dissolution behavior, and estimate capillary radius as a function of tablet porosity. The estimations generated by the proposed models consistently match the experimental data across all cases investigated in this study.

Magneto-Controlled Tubular Liquid Actuators with Pore Engineering for Liquid Transport and Regulation.

Liquid manipulation using tubular actuators finds diverse applications ranging from microfluidics, printing, liquid transfer to micro-reactors. Achieving flexible and simple regulation of manipulated liquid droplets during transport is crucial for the tubular liquid actuators to perform complex and multiple functions, yet it remains challenging. Here, a facile tubular actuator for directional transport of various liquid droplets under the control of an externally applied magnetic field is presented. The surfaces of the actuator can be engineered with submillimeter-sized through-hole pores, which enables the liquid droplet to be easily modulated in the transport process. Furthermore, the liquid actuator with featured through-hole pores is expanded to function as a switch in an integrated external electric circuit by magnetically controlling the motion of a conductive liquid droplet. This work develops a strategy for regulating liquid droplets in the tubular actuation systems, which may inspire ideas for designing functional liquid actuators with potential applications in microfluidics, microchemical reaction, liquid switch, and liquid robotics.

Low ice adhesion on soft surfaces: Elasticity or lubrication effects?

HYPOTHESIS: Soft materials are promising candidates for designing passive de-icing systems. It is unclear whether low adhesion on soft surfaces is due to elasticity or lubrication, and how these properties affect the ice detachment mechanism. This study presents a systematic analysis of ice adhesion on soft materials with different lubricant content to better understand the underpinning interaction. EXPERIMENTS: The wetting and mechanical properties of soft polydimethylsiloxane with different lubricant content were thoroughly characterized by contact angle, AFM indentation, and rheology measurements. The collected information was used to understand the relationship with the ice adhesion results, obtained by using different ice block sizes. FINDINGS: Three different de-icing mechanisms were identified: (i) single detachment occurs when small ice blocks are considered, and the ice completely detaches in a single event. In the case of larger ice blocks, the reattachment of the ice block is promoted by either: (ii) stick-slip or, (iii) interfacial slippage, depending on the lubricant content. It was confirmed that the ice adhesion strength not only depends on material properties but also on experimental conditions, such as the ice dimensions. Moreover, differently than on hard surfaces, where wetting primarily determines the icephobic performance, also elasticity and lubrication should be considered on soft surfaces.

Comparative study on mechanical performance of silty soil and expansive soil below canal structures in cold regions.

Silty soil was widely used as filling soil materials for the replacement of expansive soil in cold regions. This paper presents a straightforward approach for the effects of wetting-drying-freezing-thawing cycles on mechanical behaviors of silty soil and expansive soil by laboratory tests. The results showed that the silty soil and expansive soil after 7th wetting-drying-freezing-thawing cycles presented the decreases of elastic modulus, failure strength, cohesion and angel of internal friction by 8.9 %∼12.0 %, 7.7 %∼9.0 %, 7.9 %, 4.5 % and 17.6 %∼37.0 %, 20.5 %∼29.4 %, 43.2 %, 13.0 %, respectively, indicating that wetting-drying-freezing-thawing cycles had little impact on mechanical property of silty soil and a great influence on that of expansive soil. Among them, the mechanical property attenuation ratio in the first three wetting-drying-freezing-thawing cycles accounted for over 90 % of the total. In the meantime, the micro-structure damage, surface crack characteristics and grain size distribution variations of expansive soil were all more significantly than these of silty soil exposed to wetting-drying-freezing-thawing cycles, which brought insight into the causes of the differences in mechanical properties for silty soil and expansive soil. It is found that the silty soil properties were more stable than expansive soil properties, and the silty soil is very effective for replacing the expansive soil below canal structures in cold regions.

Achieving Super-Metallophobicity on Silicon-based Ceramics at High Temperature.

As a critical concept in physical chemistry, superwettability is widely concerned in both fundamental science and practical engineering in past few decades. Despite this, investigation on high temperature superwettability is still a void, which is significant both in scientific and industrial fields. Herein, a ceramic with specific high temperature non-wetting property, Si2N2O is proposed. Compared with other materials, Si2N2O is elucidated with better practical non-wetting property against various non-ferrous metals. Combining with micro-nanostructures, the metallophobicity is further improved (contact angle >150° and contact angle hysteresis ≈0°). The extraordinary metal repellency is defined as "super-metallophobicity", which is proved to be induced by distinctive thermodynamic and dynamic wetting behavior on the rough surface. The research of super-metallophobicity not only sheds light on superwettability at high temperature, but also offers worthy insights for future potential material design in a wide range of applications, such as metallurgy, 3D printing and semiconductor industry.

Wetting of soap bubbles on topographic surfaces.

HYPOTHESIS: There is a relationship between the static contact angle of droplets and soap bubbles on flat homogeneous surfaces, therefore, it should be possible to derive a relationship between the static contact angle of a soap bubble on a periodic topographic surface and a droplet on a flat homogeneous surface. EXPERIMENTS: A free energy model of the static contact angle of soap bubbles on a topographic surface in the Cassie-Baxter state was derived. Polydimethylsiloxane surfaces of varying area fraction (0.125, 0.250, 0.500, 0.750, and 1.00) and periodic topographies (lined and pillared) were fabricated using 3D printed moulds for pattern transfer. A bubble goniometer was developed to accommodate bubbles of 40,000 ± 5,000 mm3 and 50,000 ± 5,000 mm3 volumes. Then, the static contact angle of bubbles of both volumes were measured on the varying topographic surfaces. FINDINGS: The derived predictions imply that the relationship between the static contact angle for bubbles on a flat homogeneous surface and on a composite surface, has the same form as the Cassie-Baxter equation for a droplet. The experimental results for the measured static contact angle for both bubble volumes on the varying surfaces had good agreement with the predicted trends.

Wetting Behavior-Induced Interfacial transmission of Energy and Signal: Materials, Mechanisms, and Applications.

Wetting behaviors can significantly affect the transport of energy and signal (E&S) through vapor, solid, and liquid interfaces, which has prompted increased interest in interfacial science and technology. E&S transmission can be achieved using electricity, light, and heat, which often accompany and interact with each other. Over the past decade, their distinctive transport phenomena during wetting processes have made significant contributions to various domains. However, few studies have analyzed the intricate relationship between wetting behavior and E&S transport. This review summarizes and discusses the mechanisms of electrical, light, and heat transmission at wetting interfaces to elucidate their respective scientific issues, technical characteristics, challenges, commonalities, and potential for technological convergence. The materials, structures, and devices involved in E&S transportation are also analyzed. Particularly, harnessing synergistic advantages in practical applications and constructing advanced, multifunctional, and highly efficient smart systems based on wetted interfaces is the aim to provide strategies.

Elucidating the lotus and rose-petal effects on hierarchical surfaces: Study of the effect of topographical scales on the contact angle hysteresis.

In nature, superhydrophobicity is almost systematically associated with a multiscale topography. Nevertheless, multiscale-textured natural surfaces can either produce water-repellent properties such as on the sacred lotus leaf or high liquid-to-solid adhesion such as on the rose petal. To conceive bio-inspired surfaces with self-cleaning properties, the proper contributions of each topographical scale to the wetting behavior need to be investigated. Conditions for the equilibrium of menisci produced at a given topographical scale are derived, yielding a recursion relation between each topographical scale. We introduce the equilibrium anchorage depth to quantify the penetration of water at equilibrium. To study the contact angle hysteresis (CAH), we thoroughly describe the mechanisms driving the advancing and receding motions of the triple line. Both phenomena depend on what we define as precursor advancing and receding motions. Eventually, the equilibrium, advancing and receding anchorage depths are related to the CAH. Topographical heterogeneities at a topographical subscale i are always associated with a reduced equilibrium anchorage depth and an enhanced robustness at all topographical scales of higher orders of magnitude. Eventually, it is demonstrated that advancing and receding anchorage depths are bounded by the equilibrium anchorage depth, elucidating how rose-petal-like surfaces systematically produce a high CAH.

Ultrafast and Reversible Superwettability Switching of 3D Graphene Foams via Solvent-Exclusive Plasma Treatments.

For highly active electron transfer and ion diffusion, controlling the surface wettability of electrically and thermally conductive 3D graphene foams (3D GFs) is required. Here, we present ultrasimple and rapid superwettability switching of 3D GFs in a reversible and reproducible manner, mediated by solvent-exclusive microwave arcs. As the 3D GFs are prepared with vapors of nonpolar acetone or polar water exclusively, short microwave radiation (≤10 s) leads to plasma hotspot-mediated production of methyl and hydroxyl radicals, respectively. Upon immediate radical chemisorption, the 3D surfaces become either superhydrophobic (water contact angle = ∼170°) or superhydrophilic (∼0°), and interestingly, the wettability transition can be repeated many times due to the facile exchange between previously chemisorbed and newly introduced radicals via the formation of methanol-like intermediates. When 3D GFs of different surficial polarities are incorporated into electric double-layer capacitors with nonpolar ionic liquids or polar aqueous electrolytes, the polarity matching between graphene surfaces and electrolytes results in ≥548.0 times higher capacitance compared to its mismatching at ≥0.5 A g-1, demonstrating the significance of wettability-controlled 3D GFs.

New piperidinium surfactants with carbamate fragments as effective adjuvants in insecticide compositions based on imidacloprid.

BACKGROUND: Surfactants, particularly non-ionic ones, are widely used as adjuvants in pesticide formulations due to their ability to maintain pesticide effectiveness without changing solution properties, such as pH. While non-ionic surfactants are generally low-toxic, stable, and excellent dispersants with high solubilization capabilities, they may be less effective than cationic surfactants, which offer superior surface activity, transport properties, and antimicrobial action. This study investigates the efficacy of new piperidinium surfactants with carbamate fragments as adjuvants in insecticide formulations containing imidacloprid. The efficacy of these formulations is being assessed against greenhouse whitefly, a pest known to harm cultivated and ornamental flowering plants. RESULTS: The aggregation behavior of piperidinium surfactants containing carbamate fragments was investigated, and their wetting effect was evaluated. Synthesized surfactants have lower CMC values compared to their methylpiperidinium analogue. The effect of piperidinium surfactants on the insecticide concentration on the surface and inside tomato leaves was assessed using spectrophotometric methods. It was found that the introduction of piperidinium surfactants with carbamate fragment at a concentration of 0.1% wt. allows for decrease in lethal concentration of imidacloprid up to 10 times, thereby testifying the marked increase in the effectiveness of imidacloprid against the greenhouse whitefly insect pest (Trialeurodes vaporariorum). It was shown that the main factors responsible for the enhanced efficacy of the insecticide were the ability of the surfactant to increase the concentration of imidacloprid on the leaf surfaces and improve their penetration into the plant. CONCLUSION: The presented work employed a comprehensive approach, which significantly increases the generalizability of the results obtained and provides the ability to predict the effect and target selection of adjuvants. © 2024 Society of Chemical Industry.

Study on methane degradation by microbial agents based on chelating wetting agent carriers.

Due to the low permeability characteristics of the deep gas-containing coal seam, the conventional prevention and control measures that cannot solve the problems of gas outbursts are unsatisfactory for the prevention and control of the coal and gas outbursts disaster. Therefore, in this study, a strain of methane-oxidizing bacteria M07 with high-pressure resistance, strong resistance, and high methane degradation rate was selected from coal mines. The growth and degradation abilities of M07 in chelating wetting agent solutions to assess its adaptability and find the optimal agent-to-M07 ratio. It provides a new method for integrating the reduction of impact tendency and gas pressure in deep coal mines. The experimental results show that M07 is a Gram-positive bacterium of the genus Bacillus, which has strong resistance and adaptability to high-pressure water injection. By degrading 70 mol of methane, M07 produces 1 mol of carbon dioxide, which can reduce gas pressure and reduce the risk of gas outbursts in coal mines. As the experiment proves, the best effect was achieved when the M07 concentration of the chelating wetting agent was 0.05%. The methane-oxidizing bacteria based on the chelating wetting agent as carriers prove a new prevention and control method for the integrated prevention and control of coal and gas outbursts in coal mines and also provide a new idea for microbial application in coal mine disaster control.