Research progress on degradation of biodegradable micro-nano plastics and its toxic effect mechanism on soil ecosystem.

Biodegradable plastics (BPs) are known to decompose into micro-nano plastics (BMNPs) more readily than conventional plastics (CPs). Given the environmental risks posed by BMNPs in soil ecosystems, their impact has garnered increasing attention. However, research focusing on the toxic effects of BMNPs on soils remains relatively limited. The degradation process and duration of BMNPs in soil are influenced by numerous factors, which directly impact the toxic effects of BMNPs. This highlights the urgent need for further research. In this context, this review delineates the classification of BPs, investigates the degradation processes of BPs along with their influencing factors, summarizes the toxic effects on soil ecosystems, and explores the potential mechanisms that underlie these toxic effects. Finally, it provides an outlook on related research concerning BMNPs in soil. The results indicate that specific BMNPs release additives at a faster rate during decomposition, degradation, and aging, with certain compounds exhibiting increased bioavailability. Importantly, a substantial body of research has shown that BMNPs generally manifest more pronounced toxic effects in comparison to conventional micro-nano plastics (CMNPs). The toxic effects associated with BMNPs encompass a decline in soil quality and microbial biomass, disruption of nutrient cycling, inhibition of plant root growth, and negative impacts on invertebrate reproduction, survival, and fertilization rates. The rough and complex surfaces of BMNPs contribute to increased mechanical damage to tested organisms, enhance absorption by microorganisms, and disrupt normal physiological functions. Notably, the toxic effects of BMNPs on soil ecosystems are influenced by factors including concentration, type of BMNPs, exposure conditions, degradation products, and the nature of additives used. Therefore, it is crucial to standardize detection technologies and toxicity testing conditions for BMNPs. In conclusion, this review provides scientific evidence that supports effective prevention and management of BMNP pollution, assessment of its ecological risks, and governance of BMNPs-related products.

Effective impact of nano-plastic-waste incorporated with nanotitina on the physical, mechanical and microstructural properties of white cement pastes composites for progressing towards sustainability.

Plastic waste (PW) has received a lot of attention as a possible additional material for industrial and environmental applications, particularly cement and/or concrete production for a more environmentally and economically sound use of raw materials and energy sources. PW has been investigated as an inert and/or active hydraulic filler for cement and/or concrete by numerous scientists. Plastic garbage is cheap, abundant, and takes long period of time to degrade in the eco-system (soil and water). The main goal of the ongoing research is to offer safety and efficacy by partially substituting nano-plastic waste (NPW), incorporated with nano-titania (NT), for the composition of white cement (WC). Blends are built up by substitution of WC with different ratios of NPW incorporated with fixed ratios of nano-titania (1.0 wt.%). Workability, physical, mechanical and microstructural properties have gone through laboratory and instrumental analysis. The results showed improvement in the compressive strength, density and microstructure due to the effective impact of fillers. Consequently, a decrease in total porosity, whiteness reflection (Ry) and early-rapid expansion. Eventually, the outcomes may reduce the pandemic strength, especially in the external environment, and other epidemics.

Effect of surface functional groups of polystyrene micro/nano plastics on the release of NOM from flocs during the aging process.

Currently, the hidden risk of microplastics in the coagulation process has attracted much attention. However, previous studies aimed at improving the removal efficiency of microplastics and ignored the importance of interactions between microplastics and natural organic matter (NOM). This study investigated how polystyrene micro/nano particles impact the release of NOM during the aging of flocs formed by aluminum-based coagulants Al13 and AlCl3. The results elucidated that nano-particles with small particle sizes and agglomerative states are more likely to interact with coagulants. After 7 years of floc aging, the DOC content of the nano system decreased by more than 40%, while the micron system did not change significantly. During coagulation, the benzene rings in polystyrene particles form complexes with electrophilic aluminum ions through π-bonding, creating new Al-O bonds. NOM tends to adsorb at micro/nano plastic interfaces due to hydrophobic interactions and conformational entropy. In the aging process, the structure of PS-Al13 or PS-AlCl3 flocs and the functional groups on the surface of micro/nano plastics control the absorption and release of organic matter through hydrophobic, van der Waals forces, hydration, and polymer bridging. In the system with the addition of nano plastics, several DBPs such as TCAA, DCAA, TBM, DBCM and nitrosamines were reduced by more than 50%. The reaction order of different morphological structures and surface functional groups of microplastics to Al13 and AlCl3 systems is aromatic C-H > C-OH > C-O > NH2 > aromatic CC > aliphatic C-H and C-O>H-CO> NH2 >C-OH> aliphatic C-H. The results provided a new sight to explore the effect of micro/nano plastics on the release of NOM during flocs aging.

Mechanistic study on the increase of Microcystin-LR synthesis and release in Microcystis aeruginosa by amino-modified nano-plastics.

Ecological risk of micro/nano-plastics (MPs/NPs) has become an important environmental issue. Microcystin-leucine-arginine (MC-LR) produced by Microcystis aeruginosa (M. aeruginosa) is the most common and toxic secondary metabolites (SM). However, the influencing mechanism of MPs and NPs exposure on MC-LR synthesis and release have still not been clearly evaluated. In this work, under both acute (4d) and long-term exposure (10d), only high-concentration (10 mg/L) exposure of amino-modified polystyrene NPs (PS-NH2-NPs) promoted MC-LR synthesis (32.94 % and 42.42 %) and release (27.35 % and 31.52 %), respectively. Mechanistically, PS-NH2-NPs inhibited algae cell density, interrupted pigment synthesis, weakened photosynthesis efficiency, and induced oxidative stress, with subsequent enhancing the MC-LR synthesis. Additionally, PS-NH2-NPs exposure up-regulated MC-LR synthesis pathway genes (mcyA, mcyB, mcyD, and mcyG) combined with significantly increased metabolomics (Leucine and Arginine), thereby enhancing MC-LR synthesis. PS-NH2-NPs exposure enhanced the MC-LR release from M. aeruginosa via up-regulated MC-LR transport pathway genes (mcyH) and the shrinkage of plasma membrane. Our results provide new insights into the long-time coexistence of NPs with algae in freshwater systems might pose a potential threat to aquatic environments and human health.

A review on the fate of micro and nano plastics (MNPs) and their implication in regulating nutrient cycling in constructed wetland systems.

This review discusses the micro-nano plastics (MNPs) and their interaction with physical, chemical and biological processes in a constructed wetland (CW) system that is typically used as a nature-based tertiary wastewater treatment for municipal as well as industrial applications. Individual components of the CW system such as substrate, microorganisms and plants were considered to assess how MNPs influence the CW processes. One of the main functions of a CW system is removal of nutrients like nitrogen (N) and phosphorus (P) and here we highlight the pathways through which the MNPs influence CW's efficacy of nutrient removal. The presence of morphologically (size and shape) and chemically different MNPs influence the growth rate of microorganisms important in N and P cycling, invertebrates, decomposers, and the plants which affect the overall efficiency of a CW treatment system. Certain plant species take up the MNPs, and some toxicity has been observed. This review focuses on two significant aspects: (1) the presence of MNPs in a significant concentration affects the efficiency of N and P removal, and (2) the removal of MNPs. Because MNPs reduce the enzyme activities in abundance and overproduction of ROS oxidizes the enzyme active sites, resulting in the depletion of proteins, ultimately inhibiting nitrogen and phosphorus removal within the substrate layer. The review found that the majority of the studies used sand-activated carbon (SAC), granular-activated carbon (GAC), rice straw, granular limestone, and calcium carbonate, as a substrate for CW treatment systems. Common plant species used in the CW include Phragmites, Arabidopsis thaliana, Lepidium sativum, Thalia dealbata, and Canna indica, which were also found to be dominant in the uptake of the MNPs in the CWs. The MNPs were found to affect earthworms such as Eisenia fetida, Caenorhabditis elegans, and, Enchytraeus crypticus, whereas Metaphire vulgaris were found unaffected. Though various mechanisms take place during the removal process, adsorption and uptake mechanism effectively emphasize the removal of MNPs and nitrogen and phosphorus in CW. The MNPs characteristics (type, size, and concentration) play a crucial role in the removal efficiency of nano-plastics (NPs) and micro-plastics (MPs). The enhanced removal efficiency of NPs compared to MPs can be attributed to their smaller size, resulting in a faster reaction rate. However, NPs dose variation showed fluctuating removal efficiency, whereas MPs dose increment reduces removal efficiency. MP and NPs dose variation also affected toxicity to plants and earthworms as observed from data. Understanding the fate and removal of microplastics in wetland systems will help determine the reuse potential of wastewater and restrict the release of microplastics. This study provides information on various aspects and highlights future gaps and needs for MNP fate study in CW systems.

Advances on micro/nanoplastics and their effects on the living organisms: A review.

Micro/nanoplastics (MPs) are attracting increasing attention owing to the potential threats they pose to the sustainability of the environment and the health of living organisms. Thus, a comprehensive understanding of the influence of MPs on living organisms is vital for developing countermeasures. We conducted an extensive literature search to retrieve the articles related to MPs via the Web of Science. Accordingly, 152 articles published in the last decade and in influential journals were selected to analyze the effects of MPs on plants, animals, microorganisms, and humans as well as the current status, hotspots, and trends of studies on MPs. The results showed that owing to the special characteristics of MPs and anthropogenic activities, MPs have become ubiquitous worldwide. MPs are ingested by plants and animals and enter the human body through various pathways, resulting in numerous adverse effects, such as growth inhibition, oxidative stress, inflammation, organ damage, and germ cell lesions. Moreover, they affect microorganisms by reshaping the structure and function of microbial communities and changing the spread pathway. However, microorganisms can also contribute to the degradation of MPs. With increasing evidence of the adverse effects of MPs on biota, coping with MP pollution and mitigating harmful outcomes have emerged as major challenges. This review focuses on (1) the main effects of MPs on living organisms, ranging from microorganisms to humans, (2) the current status and hotspots of studies related to MPs, and (3) the challenges and prospects of further studies on MPs.

Hydrophobicity-driven self-assembly of nanoplastics and silver nanoparticles for the detection of polystyrene microspheres using surface enhanced Raman spectroscopy.

Microplastics (MPs) and Nanoplastics (NPs) accumulated in the environment have been identified as a major global issue due to their potential harm to wildlife. Current research in the detection of MPs is well established. However, the detection of NPs remains challenging. The aim of this paper is to investigate the detection of polystyrene (PS) NPs on a super-hydrophobic substrate using surface-enhanced Raman spectroscopy (SERS) technology after high-speed centrifugation of PS NPs and AgNPs. The hydrophobic substrate reduces the contact area of droplet, concentrating PS NPs and AgNPs on a small spot, which eliminates the random distribution of nano particles. The condensed PS NPs and AgNPs improve the SERS intensity, reproductivity and detection sensitivity. The results show that SERS measurement on a hydrophobic substrate could significantly improve the detection sensitivity of PS NPs, with the detection limits of PS NPs as low as 0.5 mg/L (500 nm PS NPs) and 1 mg/L (100 nm PS NPs). The study provides an effective and rapid method for the detection of NPs at trace concentration, demonstrating more possibility for the future detection of trace NPs in the aquatic environment.

Response of moulting genes and gut microbiome to nano-plastics and copper in juvenile horseshoe crab Tachypleus tridentatus.

Nanoplastics (NPs) and heavy metals are typical marine pollutants, affecting the gut microbiota composition and molting rate of marine organisms. Currently, there is a lack of research on the toxicological effects of combined exposure to horseshoe crabs. In this study, we investigated the effects of NPs and copper on the expression of molt-related genes and gut microbiome in juvenile tri-spine horseshoe crabs Tachypleus tridentatus by exposing them to NPs (100 nm, 104 particles L-1) and/or Cu2+ (10 µgL-1) in seawater for 21 days. Compared with the control group, the relative mRNA expression of ecdysone receptor (EcR), retinoid x receptor (RXR), calmodulin-A-like isoform X1 (CaM X1), and heat shock 70 kDa protein (Hsp70) were significantly increased under the combined stress of NPs and Cu2+. There were no significant differences in the diversity and abundance indices of the gut microbial population of horseshoe crabs between the NPs and/or Cu2+ groups and the control group. According to linear discriminant analysis, Oleobacillus was the most abundant microorganism in the NPs and Cu2+ stress groups. These results indicate that exposure to either NPs stress alone or combined NPs and Cu2+ stress can promote the expression levels of juvenile molting genes. NPs exposure has a greater impact on the gut microbial community structure of juvenile horseshoe crabs compared to Cu2+ exposure. This study is helpful for predicting the growth and development of horseshoe crabs under complex environmental pollution.

Global Research Activities on Micro(nano)plastic Toxicity to Earthworms.

Micro(nano)plastics are emerging contaminants that have been shown to cause various ecotoxicological effects on soil biota. Earthworms, as engineers of the ecosystem, play a fundamental role in soil ecosystem processes and have been used as model species in ecotoxicological studies. Research that evaluates micro(nano)plastic toxicity to earthworms has increased greatly over the last decade; however, only few studies have been conducted to highlight the current knowledge and evolving trends of this topic. This study aims to visualize the research status and knowledge structure of the relevant literature. Bibliometrics and visualization analyses were conducted using co-citations, cooperation networks and cluster analysis. The results showed that micro(nano)plastic toxicity to earthworms is an emerging and increasingly popular topic, with 78 articles published from 2013 to 2022, the majority of which were published in the last two years. The most prolific publications and journals involved in this topic were also identified. In addition, the diversity of cooperative relationships among different countries and institutions confirmed the evolution of this research field, in which China contributed substantially. The high-frequency keywords were then determined using co-occurrence analysis, and were identified as exposure, bioaccumulation, soil, pollution, toxicity, oxidative stress, heavy metal, microplastic, Eisenia foetida and community. Moreover, a total of eight clusters were obtained based on topic knowledge clustering, and these included the following themes: plastic pollution, ingestion, combined effects and the biological endpoints of earthworms and toxic mechanisms. This study provides an overview and knowledge structure of micro(nano)plastic toxicity to earthworms so that future researchers can identify their research topics and potential collaborators.

Sustainable development goals for industry, innovation, and infrastructure: demolition waste incorporated with nanoplastic waste enhanced the physicomechanical properties of white cement paste composites.

The COVID-19 pandemic significantly impacts the increase in plastic waste from food packaging, masks, gloves, and personal protective equipment (PPE), resulting in an environmental disaster, if collected, processed, transported, or disposed inappropriately. Plastic waste has a very long deterioration time in the environment (soil and water), cheap, and plentiful. Additionally, construction waste disposal is a process that transfers debris to a state that does lead to any sustainable or environmental problems. The core objective of this current research work is to provide safety and efficacy by partial substitution of both ultrafine demolition waste (UDW), incorporated with nanoplastic waste (NPW), for eco-white cement (E-WC) composition. E-WC is designed by partially substituted WC with UDW (1.0, 5.0, 10.0, 15.0, and 20.0 wt.%); incorporated with NPW (1.0 and 3.0 wt.%); to adequately protect people and the environment over long periods. The context examines the high performance, physicomechanical properties and high durability of blends as presences of silica in UDW proposed a hydraulic filler material, plus; high surface area of NPW. The microstructure and workability are characterized by X-Ray Fluorescence (XRF), Scanning Electron Microscope (SEM), and Transmission Electron Microscope (TEM) measurements. The record results show greatly enhanced in the mechanical strength due to the combination of NPW and UDW (active silica). With the presence of NPW and UDW in WC matrix, the highest level of crystallization formed consequently a decrease in whiteness reflection (Ry) and total porosity. In summary, WC blend with NPW and UDW reflects better workability and energy saving qualities, which are economical and environmentally beneficial and may result in decreased construction budget and improve a long-term raw material sustainability.

Aging of poly (lactic acid)/poly (butylene adipate-co-terephthalate) blends under different conditions: Environmental concerns on biodegradable plastic.

Biodegradable plastics (BPs) have been used to replace conventional plastics owing to their environmental harmless and ease of degradation. However, the aging processes of BPs in different environments remain unclear. In this study, we used poly (lactic acid)/poly (butylene adipate-co-terephthalate) (PLA/PBAT) films as model BPs and investigated the 30-d aging behavior of PLA/PBAT films under four conditions (i.e., air without ultraviolet (UV) irradiation, water without UV irradiation, air with UV irradiation, and water with UV irradiation). Our results showed that the aging of PLA/PBAT films was insignificant in all groups except the water with UV irradiation group. In the physical characterization, the PLA/PBAT films exhibited layered structures in water with UV irradiation condition, and the submicron- and nano-sized particles adhered to the bigger-sized fragments. In the chemical characterization, the carbonyl index (CI) of PLA/PBAT films in water with UV irradiation condition decreased from 3.84 to 1.36, and the oxygen-to-carbon (O/C) ratio reached a maximum of 1.78 at 20 d and declined to 0.49 at 30 d, indicating that the oxygen-containing functional groups underwent bond breaking and showed a rapid aging process. This is mainly attributed to the combined effect of hydrolysis and photolysis increases the contact area of PLA/PBAT films and accelerates the aging process. Furthermore, based on two-dimensional correlation spectroscopy (2D-COS) analysis, we suggest that free radicals generated in water with UV irradiation conditions also accelerate the aging process of PLA/PBAT films. This study explored the aging processes of PLA/PBAT films under different conditions, which could aid in clarifying the environmental behavior and provide further information to assess the potential risks of BPs.

Endocrine disrupting effect and reproductive toxicity of the separate exposure and co-exposure of nano-polystyrene and diethylstilbestrol to zebrafish.

The co-occurrence of nanoplastics and other pollutants in the environment has gotten a lot of attention, but information on the biological toxicity of their co-exposure was limited. This study aims to reveal the endocrine disrupting effect and reproductive toxicity of nano-polystyrene (NPS) and diethylstilbestrol (DES) to zebrafish under separate and combined exposure. Results indicated that NPS and DES exposure in isolation reduced the hepatosomatic index and gonadosomatic index, and altered the cell maturity in gonads in both cases. Even worse, the co-exposure of NPS and DES exacerbated the damage to the liver and gonads of fish. The two pollutants individually inhibited the secretion of sex hormones and vitellogenin. The inhibition effect of DES was especially dose-dependent, while NPS had weaker effect than DES. Their combined action on the secretion of sex hormones and vitellogenin exhibited additive effect. However, NPS did not affect the content of thyroid hormones in fish, and also had no significant effect on the reduction of thyroid hormone caused by DES exposure. Furthermore, their co-exposure decreased the cumulative eggs from 1031 to 306, and the spawning number from 12 to 8. The fertilization rate and hatchability rete of eggs were reduced by 30.9% and 40.4%, respectively. The abnormality rate of embryos was 65.0%, significantly higher than in separate DES and NPS groups (55.7% and 30.8% respectively). The abnormal development of offspring was mainly pericardial cyst, spinal curvature, and growth retardation.

Cytotoxicity and pro-inflammatory effect of polystyrene nano-plastic and micro-plastic on RAW264.7 cells.

Numerous studies have shown that exposure to micro- or nano-plastics led to the cell viability and function of macrophages in the intestine tissue might be one possible mechanism. This study investigated the cytotoxicity and pro-inflammatory effect of 80 nm polystyrene-nano-plastic (PS-NP) and 3 µm PS-micro-plastic (PS-MP) on mouse macrophages RAW264.7 cells. Our results showed that exposure to PS-NP or PS-MP induced apoptosis of cells at 5 or 10 µg/mL, respectively. Besides, PS-NP enhanced the secretion of inflammatory cytokines (Tumor necrosis factor-α, Interleukin-6 and Interleukin-10) with the lowest effective concentration (LOEC) of 1, 0.01, and 0.01 µg/mL, respectively. PS-MP enhanced secretion of TNF-α and IL-10 with the LOEC of 1 and 0.01 µg/mL, respectively. We further studied the possible mechanisms of the effects of PS-NP or PS-MP on RAW264.7 cells. We found they might cause cytotoxicity and inflammatory effects by producing reactive oxygen species and nitric oxide in the cells. Accordingly, our results demonstrated that PS-NP and PS-MP had cytotoxicity and pro-inflammatory effect on macrophages, which might further lead to intestinal inflammation. Moreover, we revealed that the PS-NP had more potent adverse impacts on macrophages than PS-MP.

Optimized High-Content Imaging Screening Quantifying Micronuclei Formation in Polymer-Treated HaCaT Keratinocytes.

Research on nano- and micro-plastic particles (NMPPs) suggests their potential threat to human health. Some studies have even suggested genotoxic effects of NMPP exposure, such as micronuclei (MN) formation, while others found the opposite. To clarify the ability of NMPP to induce MN formation, we used non-malignant HaCaT keratinocytes and exposed these to a variety of polystyrene (PS) and poly methyl methacrylate (PMMA) particle types at different concentrations and three different sizes. Investigations were performed following acute (one day) and chronic exposure (five weeks) against cytotoxic (amino-modified NMPPs) and genotoxic (methyl methanesulfonate, MMS) positive controls. An optimized high-content imaging workflow was established strictly according to OECD guidelines for analysis. Algorithm-based object segmentation and MN identification led to computer-driven, unsupervised quantitative image analysis results on MN frequencies among the different conditions and thousands of cells per condition. This could only be realized using accutase, allowing for partial cell detachment for optimal identification of bi-nucleated cells. Cytotoxic amino-modified particles were not genotoxic; MMS was both. During acute and long-term studies, PS and PMMA particles were neither toxic nor increased MN formation, except for 1000 nm PS particles at the highest concentration of unphysiological 100 µg/mL. Interestingly, ROS formation was significantly decreased in this condition. Hence, most non-charged polymer particles were neither toxic nor genotoxic, while aminated particles were toxic but not genotoxic. Altogether, we present an optimized quantitative imaging workflow applied to a timely research question in environmental toxicity.

Investigating transport kinetics of polystyrene nanoplastics in saturated porous media.

Understanding the fate and transport of polystyrene nanoparticles (PSNPs) in porous media under various conditions is necessary for evaluating and predicting environmental risks caused by microplastics. The transport kinetics of PSNPs are investigated by column experiment and numerical model. The surface of DLVO interaction energy is calculated to analyze and predict the adsorption and aggregation of PSNPs in porous media, which the critical ionic strength of PSNPs can be accurately investigated. The results of the DLVO energy surface suggest that when the concentration of Na+ increases from 1 mM to 50 mM, the DLVO energy barrier of PSNPs-silica sand (SS) decreases from 78.37 kT to 5.46 kT. As a result, PSNPs are easily adsorbed on the surface of SS and the mobility of PSNPs is reduced under the condition of a high concentration of Na+ (PSNPs recovery rate decreases from 62.16% to 3.65%). When the concentration of Ca2+ increases from 0.1 mM to 5 mM, the DLVO energy barrier of PSNPs-SS decreases from 12.10 kT to 1.90 kT, and PSNPs recovery rate decreases from 82.46% to 4.27%. Experimental and model results showed that PSNPs mobility is enhanced by increasing initial concentration, flow velocity and grain size of SS, while the mobility of PSNPs with larger particle diameter is lower. Regression analysis suggests that kinetic parameters related to PSNPs mobility are correlated with DLVO energy barriers. The environmental behavior and mechanism of PSNPs transport in porous media are further investigated in this study, which provides a scientific basis for the systematic and comprehensive evaluation of the environmental risk and ecological safety of nano-plastic particles in the groundwater system.

Effects of clay minerals on the transport of polystyrene nanoplastic in groundwater.

Microplastics are widely detected in the soil-groundwater environment, which has attracted more and more attention. Clay mineral is an important component of the porous media contained in aquifers. The transport experiments of polystyrene nanoparticles (PSNPs) in quartz sand (QS) mixed with three kinds of clay minerals are conducted to investigate the effects of kaolinite (KL), montmorillonite (MT) and illite (IL) on the mobility of PSNPs in groundwater. Two-dimensional (2D) distributions of DLVO interaction energy are calculated to quantify the interactions between PSNPs and three kinds of clay minerals. The critical ionic strengths (CIS) of PSNPs-KL, PSNPs-MT and PSNPs-IL are 17.0 mM, 19.3 mM and 21.0 mM, respectively. Experimental results suggest KL has the strongest inhibition effect on the mobility of PSNPs, followed by MT and IL. Simultaneously, the change of ionic strength can alter the surface charge of PSNPs and clay minerals, thus affecting the interaction energy. Experimental and model results indicate both the deposition rate coefficient (k) and maximum deposition (Smax) linearly decrease with the logarithm of the DLVO energy barrier, while the mass recovery rate of PSNPs (Rm) exponentially increases with the logarithm of the DLVO energy barrier. Therefore, the mobility and associated kinetic parameters of PSNPs in complex porous media containing clay minerals can be predicted by 2D distributions of DLVO interaction energy. These findings could help to gain insight into understanding the environmental behavior and transport mechanism of microplastics in the multicomponent porous media, and provide a scientific basis for the accurate simulation and prediction of microplastic contamination in the groundwater system.

Biochar alters chemical and microbial properties of microplastic-contaminated soil.

The occurrence of microplastics (MPs) in soils can negatively affect soil biodiversity and function. Soil amendments applied to MP-contaminated soil can alter the overall soil properties and enhance its functions and processes. However, little is known about how soil amendments improve the quality of MP-contaminated soils. Thus, the present study used a microcosm experiment to explore the potential effects of four types of biochar on the chemical and microbial properties of low-density polyethylene (LDPE) MP-contaminated soil under both drought and well-watered conditions. The results show that the biochars altered soil pH, electrical conductivity (EC), available phosphorous, and total exchangeable cations (TEC) with some variability depending on the biochar type. Oilseed rape straw (OSR)-derived biochars increased soil pH, EC, and TEC under both water conditions with the highest values of 7.94, 0.54 dS m-1 and 22.0 cmol(+) kg-1, respectively. Soil enzyme activities varied under all treatments; in particular, under drought conditions, the fluorescein diacetate activity increased in soils with high temperature (700 °C) biochar. The application of soft wood pellet biochar (700 °C) to MP-contaminated soil increased urease activity by 146% under well-watered conditions. OSR-derived biochars significantly reduced soil acid phosphatase activity under both water conditions. With biochar supplementation, the diversity indices of the bacterial community increased in well-watered soil but not in soil under drought conditions. The abundance of bacterial phyla, such as Firmicutes, Proteobacteria, Actinobacteria, Dictyoglomi, and Gemmatimonadetes, was relatively high in all treatments. Biochar application resulted in negligible variations in bacterial communities under drought conditions but significant variations under well-watered conditions. The findings of this study imply that biochar can be used as a soil amendment to improve the overall soil quality of MP-contaminated soil, but its impact varies depending on the pyrolysis feedstock and temperature. Thus, selecting a suitable biochar is important for improving the soil quality in MP-contaminated soils.

Micro (nano) plastics in wastewater: A critical review on toxicity risk assessment, behaviour, environmental impact and challenges.

With millions of tonnes of plastic pollution generated every year, small-sized plastic particles, including micro- and nanoplastics, end up in freshwater systems. Due to the very small size and very large specific surface area of nanoplastics, they are known to be persistent and toxic in our environment. These particles are also known to react with other water-borne contaminants and cause acute toxicity in organisms. Nanoplastics are prone to biomagnification and can be transported to humans through various pathways. This study aims to contribute towards understanding the behaviour of nanoplastics in our environment, specifically through identification of various sources, detection techniques, toxicity estimation, health risk in humans, environmental fate, recovery and reuse, and future challenges and limitations. Detailed review on the toxic effects of nanoplastics on various organisms and their degradation rates in soil and water matrices are provided. The suitability of small- and large-scale separation techniques for the removal of nanoplastics in wastewater treatment plants is also discussed. Current challenges and future perspectives in understanding the fate and transport of nanoplastics in the environment are also discussed. Research gaps, including the development of quantification techniques, estimation of degradation mechanisms, transport in marine ecosystems, and development of sensors to examine nanoplastics in the environment, are explored. Finally, we can limit the release of nanoplastics to the environment through reduction, reuse and recycling (3 Rs) of bulk plastic products.

The effects of two sized polystyrene nanoplastics on the growth, physiological functions, and toxin production of Alexandrium tamarense.

Micro- and nano-plastics (MNPs) are increasingly prevalent pollutants in marine ecosystems and result in various deleterious effects on marine organisms. There have been studies evaluated the toxic effects of MNPs on marine microalgae, but few of them focused on the effects of MNPs on dinoflagellate species and their toxins production, which could have significant implications on human health and ecological safety in coastal areas. In this study, the common harmful algal blooms-causing dinoflagellate Alexandrium tamarense was exposed to 0.1 and 1 µm sized polystyrene nanoplastics (NPs) to investigate the responding patterns of population growth, multiple physiological functions, as well as the intracellular paralytic shellfish toxins (PSTs) productions. The results indicated the population growth, photosynthetic parameters, nutrients (nitrate and phosphate) uptake rates and extracellular carbonic anhydrase activities (CAext) were all inhibited by the two sized NPs, accompanied by the prolonged and more aggregated microalgal cells under the observation of scanning electron microscope (SEM), and the inhibition effects were more severe under 1 µm sized NPs than 0.1 µm sized NPs. Finally, we found the intracellular PSTs contents increased 73.59% exposed to 0.1 µm sized NPs while decreased 85.50% exposed to 1 µm sized NPs comparing the controls at 96 h, without significant changes of relative compositions. These results provided evidence that MNPs were toxic to A. tamarense and affected their intracellular PSTs productions within 96 h, which is critical to consider when evaluating the potential risks of MNPs in marine ecosystems.

A critical review of the environmental impacts of manufactured nano-objects on earthworm species.

The presence of manufactured nano-objects (MNOs) in various consumer or their (future large-scale) use as nanoagrochemical have increased with the rapid development of nanotechnology and therefore, concerns associated with its possible ecotoxicological effects are also arising. MNOs are releasing along the product life cycle, consequently accumulating in soils and other environmental matrices, and potentially leading to adverse effects on soil biota and their associated processes. Earthworms, of the group of Oligochaetes, are an ecologically significant group of organisms and play an important role in soil remediation, as well as acting as a potential vector for trophic transfer of MNOs through the food chain. This review presents a comprehensive and critical overview of toxic effects of MNOs on earthworms in soil system. We reviewed pathways of MNOs in agriculture soil environment with its expected production, release, and bioaccumulation. Furthermore, we thoroughly examined scientific literature from last ten years and critically evaluated the potential ecotoxicity of 16 different metal oxide or carbon-based MNO types. Various adverse effects on the different earthworm life stages have been reported, including reduction in growth rate, changes in biochemical and molecular markers, reproduction and survival rate. Importantly, this literature review reveals the scarcity of long-term toxicological data needed to actually characterize MNOs risks, as well as an understanding of mechanisms causing toxicity to earthworm species. This review sheds light on this knowledge gap as investigating bio-nano interplay in soil environment improves our major understanding for safer applications of MNOs in the agriculture environment.