The Repellent Capacity against Sitophilus zeamais (Coleoptera: Curculionidae) and In Vitro Inhibition of the Acetylcholinesterase Enzyme of 11 Essential Oils from Six Plants of the Caribbean Region of Colombia.

The repellent capacity against Sitophilus zeamais and the in vitro inhibition on AChE of 11 essential oils, isolated from six plants of the northern region of Colombia, were assessed using a modified tunnel-type device and the Ellman colorimetric method, respectively. The results were as follows: (i) the degree of repellency (DR) of the EOs against S. zeamais was 20-68% (2 h) and 28-74% (4 h); (ii) the IC50 values on AChE were 5-36 µg/mL; likewise, the %inh. on AChE (1 µg/cm3 per EO) did not show any effect in 91% of the EO tested; (iii) six EOs (Bursera graveolens-bark, B. graveolens-leaves, B. simaruba-bark, Peperomia pellucida-leaves, Piper holtonii (1b*)-leaves, and P. reticulatum-leaves) exhibited a DR (53-74%) ≥ C+ (chlorpyrifos-61%), while all EOs were less active (8-60-fold) on AChE compared to chlorpyrifos (IC50 of 0.59 µg/mL). Based on the ANOVA/linear regression and multivariate analysis of data, some differences/similarities could be established, as well as identifying the most active EOs (five: B. simaruba-bark, Pep. Pellucida-leaves, P. holtonii (1b*)-leaves, B. graveolens-bark, and B. graveolens-leaves). Finally, these EOs were constituted by spathulenol (24%)/ß-selinene (18%)/caryophyllene oxide (10%)-B. simaruba; carotol (44%)/dillapiole (21%)-Pep. pellucida; dillapiole (81% confirmed by 1H-/13C-NMR)-P. holtonii; mint furanone derivative (14%)/mint furanone (14%)-B. graveolens-bark; limonene (17%)/carvone (10%)-B. graveolens-leaves.

Phenazine 1-carboxylic acid Producing Seed Harbored Endophytic Bacteria from Cultivated Rice Variety of Kerala and Its Broad Range Antagonism to Diverse Plant Pathogens.

Endophytic microorganisms residing within the diverse parts of plants play a significant role in the plant growth and defense response. In the case of the vertically transmitted seed-borne endophytes, they form the promising initiator of the juvenile plant microbiome by supporting the growth and establishment of the seedlings. Hence, the current study emphasizes the isolation and screening of plant beneficial traits of seed endophytes from the cultivated rice variety Jyothi of Kerala, India. Among the 14 bacterial endophytes obtained in the study, the isolate S3 was found to have promising activity against the phytopathogens such as Fusarium oxysporum, Pythium aphanidermatum, Pythium myriotylum, Phytophthora infestans, Rhizoctonia solani, Colletotrichum acutatum, and Sclerotium rolfsii. The isolate S3 was further identified as Paenibacillus polymyxa by the 16S rRNA-based sequence analysis. Furthermore, the isolate was confirmed for its capability for hydrogen cyanide (HCN) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, biofilm formation, and nitrogen fixation. The P. polymyxa S3 was also found to have the potential to provide post-harvest protection to the rice kernels from Sclerotium rolfsii. By the LC-MS/MS analysis, the organism was confirmed for the production of phenazine 1-carboxylic acid which could be the prime chemical basis of its antifungal activity. The in vivo plant growth evaluation has also demonstrated the root length enhancement effect of P. polymyxa S3 in Vigna unguiculata. Here, the root length of P. polymyxa S3-treated plant was enhanced to 12.44 ± 0.58223 cm when compared with distilled water control (10.261 ± 0.38151 cm) and the observed change was statistically significant as per the analysis of variance at P value less than 0.05. Based on all these properties, the isolated P. polymyxa S3 could be considered as a promising agent to be used for the development of competent plant probiotic formulations.