Long exposure to a mixture of endocrine disruptors prediposes the ventral prostate of rats to preneoplastic lesions.

Endocrine disruptors (ED) are compounds dispersed in the environment that modify hormone biosynthesis, affecting hormone-dependent organs such as the prostate. Studies have only focused on evaluating the effects of ED alone or in small groups and short intervals and have not adequately portrayed human exposure. Therefore, we characterized the prostate histoarchitecture of rats exposed to an ED mixture (ED Mix) mimicking human exposure. Pregnant females of the Sprague-Dawley strain were randomly distributed into two experimental groups: Control group (vehicle: corn oil, by gavage) and ED Mix group: received 32.11 mg/kg/day of the ED mixture diluted in corn oil (2 ml/kg), by gavage, from gestational day 7 (DG7) to post-natal day 21 (DPN21). After weaning at DPN22, the male pups continued to receive the complete DE mixture until they were 220 days old when they were euthanized. The ED Mix decreased the epithelial compartment, increased the fractal dimension, and decreased glandular dilation. In addition, low-grade prostatic intraepithelial neoplasia was observed in addition to regions of epithelial atrophy in the group exposed to the ED Mix. Exposure to the mixture decreased both types I and III collagen area in the stroma. We concluded that the ED Mix was able to cause alterations in the prostatic histoarchitecture and induce the appearance of preneoplastic lesions.

Immunoendocrine abnormalities in the male reproductive system during experimental pulmonary tuberculosis.

INTRODUCTION: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) that mainly affects the lungs. Along the course of pulmonary TB there are remarkable changes in the production of cytokines that cause endocrine changes. So far, it is not known the physiological and histological changes in the male reproductive system during pulmonary TB. OBJECTIVE: To investigate whether pulmonary TB produces histological alterations of the BALB/c mice reproductive organs, as well as abnormalities in spermatogenesis, serum testosterone concentrations and expression of testicular cytokines. METHODS: BALB/c mice were infected intratracheally with high dose Mtb strain H37Rv. Groups of six non infected and infected animals were euthanized on days 1, 3, 7, 14, 21, 28, 60, 90 and 120 post-infection. Bacillary loads were determined by counting colony forming units (CFUs) in lungs, testes, prostate and seminal vesicles. Histological sections were obtained from the same organs. Spermatozoids number and quality were assessed by spermatobioscopy. Serum testosterone concentrations were determined by radioimmunoanalysis (RIA) in control and infected mice in each time of sacrifice. RESULTS: Mtb only grew in lung tissue. Serum androgens showed a trend to decrease in the infected mice compared to the healthy animals, the difference turn into statistically significance at post infection day 120. The weight of the testis was not modified throughout the study, and no histopathological changes were found. However, we detected a significant decrease in the weight of the seminal vesicles and prostate starting at 28 days post-infection. Atrophy of the seminal vesicles and prostate epithelia were significant, beginning after 60 days of infection. Spermatobioscopy revealed hypospermia in the later stages of the disease. We have observed in the testes a local significant disbalance on the cytokine profile (increase of IL-6 and decrease of IL-10 and TGF-b levels) together with a very significant reduction of the body weight during late pulmonary TB. CONCLUSION: Pulmonary TB affects the histophysiology of the male reproductive system due to hormonal changes, an imbalance of pro-inflammatory cytokine profile, and a wasting syndrome during late disease.

Heparanase 1 involvement in prostate physiopathology.

The prostate is a compound exocrine gland of the male reproductive tract universally present in mammals. It is highly responsive to androgen and can be committed by a variety of pathological complications as prostatitis, benign, and malignant proliferative changes, which may be intensified by aging. Prostate intensively turnover its extracellular matrix (ECM) either at homeostasis or disease which includes a dynamically change of glycosaminoglycan composition during the life of an individual. Among the different enzymes playing a role in such changes, heparanase-1 is responsible for cleaving heparan sulfate (HS) at a limited number of sites, clearly involved in tissue remodeling. Its activity has been strongly implicated in cell invasion associated with cancer metastasis, a consequence of the structural modification that loosens the ECM barrier. In the present review we focuses in some aspects of the prostate physiology and diseases, particular prostate cancer, evidencing how the HPSE-1 activity encompasses the relationship of both processes.

Synthesis and Identification of Pregnenolone Derivatives as Inhibitors of Isozymes of 5α-Reductase.

Hyperplasia of the prostate gland and prostate cancer have been associated with high levels of serum 5α-dihydrotestosterone. This steroid is formed from testosterone by the activity of the enzyme 5α-reductase (5α-R) present in the prostate. Thus, inhibition of this enzyme could be a goal for therapies to treat these diseases. This study reports the synthesis and effects of five different 21-esters of pregnenolone derivatives as inhibitors of 5α-R types 1 and 2. The activity of these steroidal compounds was determined using in vivo and in vitro experiments. The results indicate that of the five steroids studied, the 21(p-fluoro)benzoyloxypregna-4,16-diene-3,6,20-trione derivative, whose structure has not yet been reported, has the best molecular conformation to inhibit the in vitro activity of both types of 5α-R. In addition, this steroid also displayed activity in vivo. Apparently, its pharmacological effect was increased by the presence of a keto group at C-6, because this group decreased the possibility that the steroid would be metabolized by hepatic enzymes. In addition, the double bond present at C-4 of this compound also enhanced its inhibitory activity on 5α-R, and the C-21 ester moiety increased its liphophilicity. Therefore, its solubility in the cell membrane and its pharmacological activity were both increased.

Gross anatomical, histological and histochemical studies on the postnatal development of the prostate gland of Gaddi goat / Estudios anatómicos macroscópicos, histológicos e histoquímicos sobre el desarrollo postnatal de la glándula prostática de la cabra Gaddi

The study was conducted on the prostate gland of Gaddi goat from one day old to more than five years of age divided into three groups viz; prepubertal (1 day old to < 18 months of age), pubertal (18 months to < 5 yrs of age) and postpubertal (> 5 yrs of age). The prostate comprised of corpus prostatae, a band like structure close to the junction of vesicular gland with the urethra, and the pars disseminate which extended in urethra well from its origin to the point of duct of bulbourethral gland. Microscopically, the corpus prostatae comprised of two compact glandular masses lying one over the other, dorsally over the origin of pelvic urethra covered by a thick fibro-reticular capsule. The gland composed of end pieces (luminated and non-luminated acini) and ducts arranged in lobulated fashion. The thickness of inter and intralobular connective tissue decreased with increased age at the expense of the growth of paraenchyma. With age the luminated secretory end pieces increased, while the non-luminatedend pieces decreased in the lobules of the gland. Glandular parenchyma were rich in mucous components by 6 month age serous and mucous components became almost equal and at 12 month age majority of the secretory end pieces turned in to serous type. The excretory ducts which were lined by stratified cuboidal epithelium in one day old kids changed to transitional epithelium in late prepubertal and pubertal animals. The glandular elements were PAS and Best's carmine reactive while interstitial connective tissue was non reactive. Mild alkaline phosphatase reaction was evident in the interstitial connective tissue cells. A strong acid phosphatase reaction was evident in the endothelium. BPB reaction for protein was moderate to intense. Ducts and acini were PAS and Alcian Blue reactive. The reaction for glycogen and AMPS contents in the gland increased with age. It was very intense in the pubertal animals. Moderate DNA activity, mild to moderate alkaline and acid...

Se realizó un estudio de la glándula próstatica de la cabra Gaddi, desde el día uno de nacimiento hasta después de los cinco años de edad, divididos grupos prepuberal (1 día a 18 meses de edad), puberal (18 meses a <5 años de edad) y post-púberes (más de 5 años de edad). La próstata estaba constituida por un cuerpo prostático, estructura como una banda cercana a la unión de la glándula vesicular con la uretra, y una pars diseminada la cual se extendía en las paredes de la uretra desde su origen hasta el punto de los conductos de la glándula bulbouretral. Microscópicamente, el cuerpo prostático está compuesto por dos masas compactas glandulares situadas una sobre la otra, dorsalmente sobre el origen de la uretra pélvica cubierta por una gruesa cápsula fibro-reticular. La glándula compuesta por piezas terminales (acinos luminal y no luminal) y conductos dispuestos en forma lobulada. El espesor del tejido conectivo inter e intralobulillar disminuyó con el aumento de la edad a expensas del crecimiento del parénquima. Con la edad aumentaron las piezas terminales secretoras luminales, mientras que en las piezas terminales no luminales, disminuyeron en los lóbulos de la glándula. El parénquima glandular era abundante en componentes mucosos a la edad de 6 meses, mientras que los componentes serosos y mucosas se equilibraron a los 12 meses de edad; la mayoría de las piezas finales secretoras se transformaron a tipo serosa. Los conductos excretores que estaban revestidos por epitelio cúbico estratificado en el día uno de las crías, cambió a epitelio de transición en los animales prepúberes y púberes. Los elementos glandulares fueron reactivos al PAS y al carmín de Best, mientras que el tejido conectivo intersticial no fue reactivo. Una reacción leve a la fosfatasa alcalina fue evidente en las células del tejido conectivo intersticial, mientras que una reacción fuerte se evidenció en el endotelio. La reacción de BPB para proteína fue de moderada a intensa. Los conductos...

Morphology of the male agouti accessory genital glands (Dasyprocta prymnolopha Wagler, 1831) / / Morfologia das glândulas genitais acessórias em cutias (Dasyprocta prymnolopha Wagler, 1831)

The morphology of the accessory genital glands of the male agouti was studied in twenty-three animals that were raised in captivity. Twenty animals had their genital glands dissected in situ for macroscopic description. The samples of each gland were recovered, embedded in paraffin, sliced and stained by Hematoxylin-Eosin technique. It was founded four pairs of glands: the vesicular glands, the coagulating glands, the prostate and the bulbourethral glands. Histological characteristics of the vesicular, coagulating and prostate glands showed similar morphology, within the pseudostratified columnar epithelium. The tubulo-alveolar type of the bulbourethral glands showed a lack of connective tissue among the tubules, a small amount of red stained presented it the cytoplasm, and the presence of vacuoles in the tissue. This study concluded that the agouti showed to have similar morphological aspect described in the others species of rodents.(AU)

A morfologia das glândulas genitais acessórias de cutias foram estudados em 23 animais criados em cativeiros. Vinte animais tiveram suas glândulas genitais dissecadas in situ para as descrições macroscópicas. Para o estudo microscópico foram utilizados três animais. Os fragmentos de cada glândula foram embebidos em parafina, seccionados e corados em hematoxilina e eosina. Foram encontrados quatro pares de glândulas: vesiculares, coaguladoras, próstata e bulbouretrais. As características histológicas da glândula vesicular, coaguladora e próstata mostraram morfologia similar, com epitélio colunar pseudoestratificado. O tipo tuboalveolar da glândula bulbouretral mostrou uma deficiência de tecido conjuntivo, citoplasma pouco corado e presença de vacúolos. Este estudo concluiu que a cutia apresenta as mesmas características morfológicas das glândulas genitais acessórias encontradas em roedores.(AU)

Morphology of the male agouti accessory genital glands (Dasyprocta prymnolopha Wagler, 1831) / / Morfologia das glândulas genitais acessórias em cutias (Dasyprocta prymnolopha Wagler, 1831)

The morphology of the accessory genital glands of the male agouti was studied in twenty-three animals that were raised in captivity. Twenty animals had their genital glands dissected in situ for macroscopic description. The samples of each gland were recovered, embedded in paraffin, sliced and stained by Hematoxylin-Eosin technique. It was founded four pairs of glands: the vesicular glands, the coagulating glands, the prostate and the bulbourethral glands. Histological characteristics of the vesicular, coagulating and prostate glands showed similar morphology, within the pseudostratified columnar epithelium. The tubulo-alveolar type of the bulbourethral glands showed a lack of connective tissue among the tubules, a small amount of red stained presented it the cytoplasm, and the presence of vacuoles in the tissue. This study concluded that the agouti showed to have similar morphological aspect described in the others species of rodents.

A morfologia das glândulas genitais acessórias de cutias foram estudados em 23 animais criados em cativeiros. Vinte animais tiveram suas glândulas genitais dissecadas in situ para as descrições macroscópicas. Para o estudo microscópico foram utilizados três animais. Os fragmentos de cada glândula foram embebidos em parafina, seccionados e corados em hematoxilina e eosina. Foram encontrados quatro pares de glândulas: vesiculares, coaguladoras, próstata e bulbouretrais. As características histológicas da glândula vesicular, coaguladora e próstata mostraram morfologia similar, com epitélio colunar pseudoestratificado. O tipo tuboalveolar da glândula bulbouretral mostrou uma deficiência de tecido conjuntivo, citoplasma pouco corado e presença de vacúolos. Este estudo concluiu que a cutia apresenta as mesmas características morfológicas das glândulas genitais acessórias encontradas em roedores.

Radiographie avaliation of prostatic conditions and prostatectomy simplification in dog / Avaliação radiográfica das condições prostáticas e simplificação da técnica da prostatectomia em cão

The author valves the conditions of the prostate and primordially of the urethra, across contrastic urethocistography with hyorosoluble iodate compounds. To presents modification in the technica of prostatectomy, primordially when the perineal hernia reduction, proposing of the departure of the orgasm in parallel layers without attain it. Was studied 3 experimental cases and 6 dogs that carrier prostatic pathology, and from thence was selecting the material that was utilize for document, both before and after the surgery

Avalia-se as condições da próstata, e principalmente uretra, através da uretrocistografia contrastada com compostos iodados hidrossolúveis. Apresenta modificação na técnica da prostatectomia principalmente quando da redução da hérnia perineal, propondo a retirada do órgão em camadas paralelas à uretra sem atingi-la. Foram estudados 3 casos experimentais e 6 de cães portadores de patologia prostática dos quais foi selecionado o material utilizado para documentação, tanto antes como depois da cirurgia.

Radiographie avaliation of prostatic conditions and prostatectomy simplification in dog / Avaliação radiográfica das condições prostáticas e simplificação da técnica da prostatectomia em cão

The author valves the conditions of the prostate and primordially of the urethra, across contrastic urethocistography with hyorosoluble iodate compounds. To presents modification in the technica of prostatectomy, primordially when the perineal hernia reduction, proposing of the departure of the orgasm in parallel layers without attain it. Was studied 3 experimental cases and 6 dogs that carrier prostatic pathology, and from thence was selecting the material that was utilize for document, both before and after the surgery

Avalia-se as condições da próstata, e principalmente uretra, através da uretrocistografia contrastada com compostos iodados hidrossolúveis. Apresenta modificação na técnica da prostatectomia principalmente quando da redução da hérnia perineal, propondo a retirada do órgão em camadas paralelas à uretra sem atingi-la. Foram estudados 3 casos experimentais e 6 de cães portadores de patologia prostática dos quais foi selecionado o material utilizado para documentação, tanto antes como depois da cirurgia.