Conservation and restoration of riverine spawning habitats require fine-scale functional connectivity and functional heterogeneity.

A severe extinction crisis of migratory fish caused by extensive hydropower development and climate change has attracted widespread environmental concern. Conserving and restoring riverine spawning habitat for migratory species is advantageous for population recovery. Depending on the reproductive characteristics of fish with adhesive eggs, functionally heterogeneous spawning habitats are required to support different stages of reproductive activity. However, few aquatic assessment models are available to consider the fine-scale functional connectivity between heterogeneous spawning habitats. This study developed a function-based framework that linked fine-scale functional connectivity modeling to habitat quality evaluations for the population recovery of migratory fish. The function path tree (FPT) model within the framework could identified the spatiotemporal dynamics of fine-scale connectivity patterns by emphasizing the attribute-dependence of patch arrangements. Here, we used the Chinese sturgeon, a well-known endangered anadromous fish producing adhesive eggs in the Yangtze River, as an example to demonstrate the applicability of the framework. Additionally, the ecological effectiveness of river restorations to overcome the detrimental influence of climate change on discharge decrease was also investigated. Compared to prior research, our methodology effectively enhanced the predictive performance of spatiotemporal distributions and quality assessments of spawning habitats. A strong correlation was discovered between the ecological profit indicator (HQI) and the estimated fecundity (R2 = 0.941) and field-collected eggs (R2 = 0.918). The minimum spawning discharge decreased from 8400 m3/s to 7000 m3/s by substrate restoration, with the optimal HQI growth rate of 52.7 % at Q < 8400 m3/s. This work will optimize long-term conservation for imperiled migratory species and help develop strategies to build resilience to ongoing environmental changes in flow-reduced rivers.

Influence of the impoundment of the Three Gorges Reservoir on hydrothermal conditions for fish habitat in the Yangtze River.

The thermal regimes of rivers play an important role in the overall health of aquatic ecosystems. Modifications to water temperature regimes resulting from dams and reservoirs have important consequences for river ecosystems. This study investigates the impacts of the impoundment of the Three Gorges Reservoir (TGR) on the water temperature regime of fish spawning habitats in the middle reach of the Yangtze River, China. Mike 11 model is used to analyze the temporal and spatial variation of water temperatures of the expanse of 400 km along the river, from Yichang to Chenglingji. The water temperature alterations caused by the operation of the TGR are assessed with river temperature metrics. The impact on spawning habitats due to water temperature variation was also discussed in different impoundments of the TGR. The results show that the TGR has significantly altered the downstream water temperature regime, affecting the baseline deviation and phase shift of the water temperature. Such impacts on the thermal regime of the river varied with the impoundment level. The effects of the TGR on the water temperature regime decreased as the distance from the structure to the sample site increased. The water temperature regime alterations have led to the delay of the spawning times of the four famous major carp (FFMC) species. The results could be used to identify the magnitudes of water temperature alterations induced by reservoirs in the Yangtze River and provide useful information to design ecological operations for the protection of river ecosystem integrity in regulated rivers.

Realistic variations in substrate composition affect spawning preference and egg retention in river lamprey (Lampetra fluviatilis).

Egg drift from the nest is clearly an important cause of mortality in lithophilic species, but the effect of substrate composition on this process has been overlooked. Here, we investigated the role of substrate on the spawning preference and egg retention of river lamprey (Lampetra fluviatilis) during a whole breeding season in a two-option experimental setting. Despite no initial preference, the lamprey eventually favoured the most efficient substrate for egg retention. The pebbly substrate hosted 12 times as many matings as the sandy one, while blurting 20% fewer eggs.

Modelling the downstream longitudinal effects of frequent hydropeaking on the spawning potential and stranding susceptibility of salmonids.

Hydropower plant operating conditions are expected to change to be more in tandem with intermittent power production so as to meet the requirements of the Paris Agreement, which in turn may negatively impact ecological conditions downstream of the hydropower plants. The current study investigates how highly flexible hydropower operating conditions may impact several salmonid species (European grayling, Atlantic salmon and brown trout) in the River Umeälven, a major river in northern Sweden; specifically, how changes in hydropeaking frequency may affect the area of the downstream watercourse that is hydraulically suitable for spawning (potential spawning area) and how changes in spill gate closing time may affect the propensity to stranding. River hydrodynamics were modeled using the open-source solver Delft3D, with a range of hydropeaking frequencies (from 10 to 60 starts and stops per day) and a range of spill gate closing times from (1-30 min). Increasing the hydropeaking frequency caused a reduction in potential spawning area, but also a reduction in dewatering of potential spawning area at low flows. Increasing spill gate closing time caused a decrease in propensity to stranding. Effects were dependent on both species and life-stage, and declined longitudinally with distance downstream from the spillway outlet. The modelling approach used here provides an effective method for predicting likely outcomes of flexible hydropower operating conditions, taking into account fish species and life-stages present and watercourse characteristics.

Urban stormwater and crude oil injury pathways converge on the developing heart of a shore-spawning marine forage fish.

Understanding how aquatic organisms respond to complex chemical mixtures remains one of the foremost challenges in modern ecotoxicology. Although oil spills are typically high-profile disasters that release hundreds or thousands of chemicals into the environment, there is growing evidence for a common adverse outcome pathway (AOP) for the vulnerable embryos and larvae of fish species that spawn in oiled habitats. Molecular initiating events involve the disruption of excitation-contraction coupling in individual cardiomyocytes, which then dysregulate the form and function of the embryonic heart. Phenanthrenes and other three-ring (tricyclic) polycyclic aromatic hydrocarbons (PAHs) are key drivers for this developmental cardiotoxicity and are also relatively enriched in land-based urban runoff. Similar to oil spills, stormwater discharged from roadways and other high-traffic impervious surfaces contains myriad contaminants, many of which are uncharacterized in terms of their chemical identity and toxicity to aquatic organisms. Nevertheless, given the exceptional sensitivity of the developing heart to tricyclic PAHs and the ubiquitous presence of these compounds in road runoff, cardiotoxicity may also be a dominant aspect of the stormwater-induced injury phenotype in fish early life stages. Here we assessed the effects of traffic-related runoff on the embryos and early larvae of Pacific herring (Clupea pallasii), a marine forage fish that spawns along the coastline of western North America. We used the well-characterized central features of the oil toxicity AOP for herring embryos as benchmarks for a detailed analysis of embryolarval cardiotoxicity across a dilution gradient ranging from 12 to 50% stormwater diluted in clean seawater. These injury indicators included measures of circulatory function, ventricular area, heart chamber looping, and the contractility of both the atrium and the ventricle. We also determined tissue concentrations of phenanthrenes and other PAHs in herring embryos. We find that tricyclic PAHs are readily bioavailable during cardiogenesis, and that stormwater-induced toxicity is in many respects indistinguishable from canonical crude oil toxicity. Given the chemical complexity of urban runoff, non-tricyclic PAH-mediated mechanisms of developmental toxicity in fish remain likely. However, from the standpoint of managing wild herring populations, our results suggest that stormwater-driven threats to individual survival (both near-term and delayed mortality) can be understood from decades of past research on crude oil toxicity. Moreover, Pacific herring embryos are promising sentinels for water quality monitoring in nearshore marine habitats, as in situand sensitive indicators of both toxic runoff and the effectiveness of pollution reduction efforts such as green stormwater infrastructure.

Synergistic impacts of sediment generation and hydrotechnical structures related to forestry on stream fish communities.

The increase in anthropogenic sediment has become the most significant factor responsible for degrading flowing waters, preventing them from attaining the good ecological state of rivers requested by Water Framework Directive. After agriculture and mining, forest exploitation is a major source of mineral suspension. The accumulation of fine sediments may reduce fish spawning effectiveness, inhibit migration, and interfere with feeding. Streams degraded by the influx of anthropogenic sediments may be recolonised provided that ecological continuity of the dendritic river network is maintained. The present study focused on mountain stream fish communities whose catchments have undergone natural and intensified soil erosion. We tested the hypothesis that forestry practices transforming the stream habitats combined with the discontinuities created by hydrotechnical structures have had negative impacts on the entire catchment and adversely altered fish communities. The research was carried out in two catchments: i) in the national park, and ii) outside the national park, in an area with forest exploitation. It was observed that the negative effects of the dramatic increases in mineral suspension loads were synergistically aggravated by the presence of objects disturbing fluvial system continuity. The consequences of the synergy between these two factors were evident in local brown trout populations. Sites formerly inhabited by brown trout lacked downstream barriers during our study. After eliminating this parameter, our analysis revealed that channel silting also reduced brown trout abundance downstream from the barrier. The weir in the Muczny Stream inhibited ichthyofaunal re-establishment in streams prone to enhanced mineral suspension input. Forest mountain streams must be preserved also as cold water refugia, hence our results are of significance for forest management. Based on our results, the complete removal of the barrier in Muczny Stream is planned for 2020, and a substantial reduction in the inflow of mineral suspension to stream channels is strongly recommended.

Egg cannibalism in capelin Mallotus villosus at beach and deep-water spawning habitats in the north-west Atlantic Ocean.

We investigated egg cannibalism in spawning capelin Mallotus villosus on the north-east Newfoundland coast during July 2012-2014, specifically whether sex, spawning condition (i.e., spawning or spent) and spawning habitat influenced egg cannibalism. Capelin spawning in deep-water were 4.5-14 times more likely to be cannibals than those at the beach, probably due to the higher spatial overlap of spawners and conspecific eggs within this habitat relative to beaches. Males were 2.1-3.7 times more likely to be cannibals than females, but female cannibals had more eggs per stomach. Spawning fish were 1.6-1.9 times more likely to be cannibals than spent fish, but spent female cannibals had more eggs per stomach relative to spawning males and females in either habitat. Findings suggest that cannibalism may be an important foraging strategy, especially at deep-water spawning habitat, possibly extending the spawning season for males or increasing the probability of post-spawning survival for females. Although 44% of sampled females and 50% of males were cannibals, the estimated mortality due to egg cannibalism was low (0.49-2.97% of eggs produced annually), suggesting that egg cannibalism does not influence recruitment to a great extent in Newfoundland capelin.

Habitat availability and ontogenetic shifts alter bottlenecks in size-structured fish populations.

For species that utilize different habitats throughout their life cycle, the habitat limitation at a given stage can act as a bottleneck on population abundance, impacting density-dependent processes such as individual growth and survival. We explore the influence of habitat limitation on population dynamics by developing a multi-stage population model based on lake-dwelling rainbow trout (Oncorhynchus mykiss) populations where adults occupy the lake habitat but use tributaries for spawning and juvenile rearing. The model details density-dependent ecological processes and ontogenetic habitat shifts, harvest mortality, and the impact of climate on growth. We ran model simulations using a range of early life stage habitat availabilities and climatic conditions representative of the native range of rainbow trout in Canada and compared the results to empirical data. The results suggest that (1) increases in early life stage habitat leads to increases in population abundance but, due to density-dependent processes, also results in slower growing stunted populations; (2) population bottlenecks can occur at any life stage, even at the adult stage if spawning and rearing habitats are abundant; (3) when the level of competition for early life stages is increased, inter-cohort competition can lead to population cycles. The model's conclusions are further reinforced by empirical data showing a similar trend in the relationship between fish density and maximum size and providing evidence that limited early life stage habitat leads to lower fish densities and larger fish size. We provide a model that links environmental conditions to population dynamics and is useful for fisheries management and habitat conservation decisions.

Spatial patterns of Anchoveta (Engraulis ringens) eggs and larvae in relation to pCO2 in the Peruvian upwelling system.

Large and productive fisheries occur in regions experiencing or projected to experience ocean acidification. Anchoveta (Engraulis ringens) constitute the world's largest single-species fishery and live in one of the ocean's highest pCO2 regions. We investigated the relationship of the distribution and abundance of Anchoveta eggs and larvae to natural gradients in pCO2 in the Peruvian upwelling system. Eggs and larvae, zooplankton, and data on temperature, salinity, chlorophyll a and pCO2 were collected during a cruise off Peru in 2013. pCO2 ranged from 167-1392 µatm and explained variability in egg presence, an index of spawning habitat. Zooplankton abundance explained variability in the abundance of small larvae. Within the main spawning and larva habitats (6-10°S), eggs were found in cool, low-salinity, and both extremely low (less than 200 µatm) and high (more than 900 µatm) pCO2 waters, and larvae were collected in warmer, higher salinity, and moderate (400-600 µatm) pCO2 waters. Our data support the hypothesis that Anchoveta preferentially spawned at high pCO2 and these eggs had lower survival. Enhanced understanding of the influence of pCO2 on Anchoveta spawning and larva mortality, together with pCO2 measurements, may enable predictions of ocean acidification effects on Anchoveta and inform adaptive fisheries management.

Stream network geomorphology mediates predicted vulnerability of anadromous fish habitat to hydrologic change in southeast Alaska.

In rivers supporting Pacific salmon in southeast Alaska, USA, regional trends toward a warmer, wetter climate are predicted to increase mid- and late-21st-century mean annual flood size by 17% and 28%, respectively. Increased flood size could alter stream habitats used by Pacific salmon for reproduction, with negative consequences for the substantial economic, cultural, and ecosystem services these fish provide. We combined field measurements and model simulations to estimate the potential influence of future flood disturbance on geomorphic processes controlling the quality and extent of coho, chum, and pink salmon spawning habitat in over 800 southeast Alaska watersheds. Spawning habitat responses varied widely across watersheds and among salmon species. Little variation among watersheds in potential spawning habitat change was explained by predicted increases in mean annual flood size. Watershed response diversity was mediated primarily by topographic controls on stream channel confinement, reach-scale geomorphic associations with spawning habitat preferences, and complexity in the pace and mode of geomorphic channel responses to altered flood size. Potential spawning habitat loss was highest for coho salmon, which spawn over a wide range of geomorphic settings, including steeper, confined stream reaches that are more susceptible to streambed scour during high flows. We estimated that 9-10% and 13-16% of the spawning habitat for coho salmon could be lost by the 2040s and 2080s, respectively, with losses occurring primarily in confined, higher-gradient streams that provide only moderate-quality habitat. Estimated effects were lower for pink and chum salmon, which primarily spawn in unconfined floodplain streams. Our results illustrate the importance of accounting for valley and reach-scale geomorphic features in watershed assessments of climate vulnerability, especially in topographically complex regions. Failure to consider the geomorphic context of stream networks will hamper efforts to understand and mitigate the vulnerability of anadromous fish habitat to climate-induced hydrologic change.

Coupling systematic planning and expert judgement enhances the efficiency of river restoration.

Ineffectiveness of current river restoration practices hinders the achievement of ecological quality targets set by country-specific regulations. Recent advances in river restoration help planning efforts more systematically to reach ecological targets at the least costs. However, such approaches are often desktop-based and overlook real-world constraints. We argue that combining two techniques commonly used in the conservation arena - expert judgement and systematic planning - will deliver cost-effective restoration plans with a high potential for implementation. We tested this idea targeting the restoration of spawning habitat, i.e. gravel bars, for 11 rheophilic fish species along a river system in Germany (Havel-Spree rivers). With a group of local fish experts, we identified the location and extent of potential gravel bars along the rivers and necessary improvements to migration barriers to ensure fish passage. Restoration cost of each gravel bar included the cost of the action itself plus a fraction of the cost necessary to ensure longitudinal connectivity by upgrading or building fish passages located downstream. We set restoration targets according to the EU Water Framework Directive, i.e. relative abundance of 11 fish species in the reference community and optimised a restoration plan by prioritising a subset of restoration sites from the full set of identified sites, using the conservation planning software Marxan. Out of the 66 potential gravel bars, 36 sites which were mainly located in the downstream section of the system were selected, reflecting their cost-effectiveness given that fewer barriers needed intervention. Due to the limited overall number of sites that experts identified as being suitable for restoring spawning habitat, reaching abundance-targets was challenged. We conclude that coupling systematic river restoration planning with expert judgement produces optimised restoration plans that account for on-the-ground implementation constraints. If applied, this approach has a high potential to enhance overall efficiency of future restoration efforts.

Temporal and spatial mapping of red grouper Epinephelus morio sound production.

The goals of this project were to determine the daily, seasonal and spatial patterns of red grouper Epinephelus morio sound production on the West Florida Shelf (WFS) using passive acoustics. An 11 month time series of acoustic data from fixed recorders deployed at a known E. morio aggregation site showed that E. morio produce sounds throughout the day and during all months of the year. Increased calling (number of files containing E. morio sound) was correlated to sunrise and sunset, and peaked in late summer (July and August) and early winter (November and December). Due to the ubiquitous production of sound, large-scale spatial mapping across the WFS of E. morio sound production was feasible using recordings from shorter duration-fixed location recorders and autonomous underwater vehicles (AUVs). Epinephelus morio were primarily recorded in waters 15-93 m deep, with increased sound production detected in hard bottom areas and within the Steamboat Lumps Marine Protected Area (Steamboat Lumps). AUV tracks through Steamboat Lumps, an offshore marine reserve where E. morio hole excavations have been previously mapped, showed that hydrophone-integrated AUVs could accurately map the location of soniferous fish over spatial scales of <1 km. The results show that passive acoustics is an effective, non-invasive tool to map the distribution of this species over large spatial scales.