ABSTRACT
Introduction: Wheat grain filling processes under post-anthesis stress scenarios depend mainly on stem traits and remobilization of stem water-soluble carbohydrates (WSC). Methods: A diverse panel of advanced semi-dwarf spring wheat lines, representing a natural variation in stem traits (WSC content, stem diameter, peduncle length, and stem wall width), was used to identify specific traits that reliably reflect the relationship between WSC and grain yield. The panel was phenotyped under various environmental conditions: well-watered, water-limited, and heat stress in Mexico, and terminal-drought in Israel. Results: Environmental stresses reduced grain yield (from 626 g m-2 under well-watered to 213 g m-2 under heat), lower internode diameter, and peduncle length. However, stem-WSC generally peaked 3-4 weeks after heading under all environmental conditions except heat (where it peaked earlier) and expressed the highest values under water-limited and terminal-drought environments. Increased investment in internode diameter and peduncle length was associated with a higher accumulation of stem WSC, which showed a positive association with yield and kernel weight. Across all environments, there were no apparent trade-offs between increased crop investment in internode diameter, peduncle length, and grain yield. Discussion: Our results showed that selecting for genotypes with higher resource investment in stem structural biomass, WSC accumulation, and remobilization could be a valuable strategy to ameliorate grain size reduction under stress without compromising grain yield potential. Furthermore, easy-to-measure proxies for WSC (stem diameter at specific internodes and length of the last internode, i.e., the peduncle) could significantly increase throughput, potentially at the breeding scale.
ABSTRACT
Pinus pinea is an important Mediterranean species due to its adaptability and tolerance to aridity and its high-quality pine nuts. Different forest types located in Mediterranean native and non-native environments provide the opportunity to perform comparative studies on the species' response to climate change. The aims of this study were to elucidate growth patterns of the species growing in native and exotic habitats and to analyze its response to climatic fluctuations, particularly drought, in both geographical contexts. Understanding stone pine (Pinus pinea) growth responses to climate variability in native and exotic habitats by comparing natural stands and plantations may provide useful information to plan adequate management under climate change. By doing so, we enhance the understanding of P. pinea's adaptability and provide practical approaches to its sustainable management. In this study, we reconstructed and compared the stem radial growth of seven stone pine stands, two in southern Spain and five in central-southern Chile, growing under different climatic conditions. We quantified the relationships between growth variability and climate variables (total rainfall, mean temperature, and SPEI drought index). Growth was positively correlated with autumn rainfall in plantations and with autumn-winter rainfall in natural stands. Growth was also enhanced by high autumn-to-spring rainfall in the driest Chilean plantation, whereas in the wettest and coolest plantation, such correlation was found in winter and summer. A negative impact of summer temperature was found only in one of the five Chilean plantations and in a Spanish site. The correlation between SPEI and tree-ring width indices showed different patterns between and within countries. Overall, exotic plantations showed lower sensitivity to climate variability than native stands. Therefore, stone pine plantations may be useful to assist in mitigating climate change.
ABSTRACT
Water use strategies play a crucial role in the adaptive capabilities of mangroves to the saline intertidal conditions, yet the intricacies of daily water use patterns in mangrove species, which are pivotal for maintaining water balance, remain poorly understood. In this comprehensive study, we aimed to clarify the water use strategies of three co-occurring mangrove species, Avicennia marina, Aegiceras corniculatum and Kandelia obovata, through stem sap flow monitoring, leaf gas exchange, and stem diameter change measurements. Our findings revealed that the daily sap flow density (SFD) of Avicennia and Aegiceras reached the peak about one hour earlier than Kandelia. When transpiration was strong, Kandelia and Aegiceras used stem storage to meet water demand, while Avicennia synchronized stem water storage. These three mangrove species adopted cross-peak water used and unique stem water storage to regulate their water balance. In Kandelia, the daily sap flow in per sapwood area was significantly lower, while water use efficiency was significantly higher than those of Avicennia and Aegiceras, indicating that Kandelia adopted a more conservative and efficient water use strategy. Sap flow in Avicennia was the most sensitive to environmental changes, while Kandelia limited water dissipation by tightly controlling stomata. Meteorological factors (photosynthetically active radiation, vapor pressure deficit, and air temperature) were the main driving factors of sap flow. The increase of soil temperature can promote the water use of mangrove species, while the increase of salinity resulted in more conservative water use. Our results highlight the diversity of daily water use strategies among the three co-occurring mangrove species, pinpointing Kandelia as the most adaptive at navigating the changing conditions of intertidal habitats in the future climate. In conclusion, our findings provide a mesoscale perspective on water use characteristics of mangroves, and also provides theoretical basis for mangroves afforestation and ecological restoration.
ABSTRACT
Diameter and height are crucial morphological parameters of banana pseudo-stems, serving as indicators of the plant's growth status. Currently, in densely cultivated banana plantations, there is a lack of applicable research methods for the scalable measurement of phenotypic parameters such as diameter and height of banana pseudo-stems. This paper introduces a handheld mobile LiDAR and Inertial Measurement Unit (IMU)-fused laser scanning system designed for measuring phenotypic parameters of banana pseudo-stems within banana orchards. To address the challenges posed by dense canopy cover in banana orchards, a distance-weighted feature extraction method is proposed. This method, coupled with Lidar-IMU integration, constructs a three-dimensional point cloud map of the banana plantation area. To overcome difficulties in segmenting individual banana plants in complex environments, a combined segmentation approach is proposed, involving Euclidean clustering, Kmeans clustering, and threshold segmentation. A sliding window recognition method is presented to determine the connection points between pseudo-stems and leaves, mitigating issues caused by crown closure and heavy leaf overlap. Experimental results in banana orchards demonstrate that, compared with manual measurements, the mean absolute errors and relative errors for banana pseudo-stem diameter and height are 0.2127 cm (4.06%) and 3.52 cm (1.91%), respectively. These findings indicate that the proposed method is suitable for scalable measurements of banana pseudo-stem diameter and height in complex, obscured environments, providing a rapid and accurate inter-orchard measurement approach for banana plantation managers.
ABSTRACT
BACKGROUND: Rice is one of the major staples that feeds about one half of the global populations, and it is important to identify the genetic loci for the traits related to yield improvement. Lodging will cause severe yield loss when it happens, and stem diameter has been characterized as an important trait for lodging resistance. However, most QTLs for stem diameter have not been finely dissected due to their sensitivity to environmental fluctuation. RESULT: In this study, we performed QTL analysis for stem diameter using populations derived from Nipponbare (NIP) and strong culm variety YYP1, and confirmed the single and combined effect of three major QTLs by recombinant inbred lines (RILs). Based on the QTL location, we found that qWS5 is a novel QTL not well characterized before. To finely dissect the novel locus, several recombinant heterogeneous inbred families (HIFs) were selected from the RILs for linkage analysis and their derived nearly isogenic lines (NILs) were subjected to detailed trait investigation throughout different years. The HIF-NILs strategy confined the QTL to about 380 kb region supported by repeated genotype and phenotype data, and it lays the foundation for QTL cloning in the future. In addition, introgression of the QTL to an elite japonica variety SD785 was performed by successive backcrossing, and it confirmed the value of qWS5 in increasing stem diameter and other agronomic traits during rice breeding. CONCLUSIONS: We prove that qWS5 is a novel QTL with relatively stable effect for stem diameter and the QTL can be finely mapped to small region by the HIF-NILs strategy. The result will facilitate the improvement of rice lodging resistance by molecular marker assisted selection breeding.
ABSTRACT
The sunflower (Helianthus annuus L.) is one of the most essential oil crops in the world. Several component traits, including flowering time, plant height, stem diameter, seed weight, and kernel weight, determine sunflower seed and oil yield. Although the genetic mechanisms governing the variation of these yield-related traits have been studied using various approaches, genome-wide association studies (GWAS) have not been widely applied to sunflowers. In this study, a set of 342 sunflower accessions was evaluated in 2019 and 2020 using an incomplete randomized block design, and GWAS was conducted utilizing two complementary approaches: the mixed linear model (MLM) and the fixed and random model circulating probability unification (farmCPU) model by fitting 226,779 high-quality SNPs. As a result, GWAS identified a number of trait-associated SNPs. Those SNPs were located close to several genes that may serve as a basis for further molecular characterization and provide promising targets for sunflower yield improvement.
Subject(s)
Genome-Wide Association Study , Helianthus , Phenotype , Polymorphism, Single Nucleotide , Quantitative Trait Loci , Helianthus/genetics , Genome-Wide Association Study/methods , Seeds/genetics , Seeds/growth & development , Quantitative Trait, HeritableABSTRACT
Introduction: Soybean stem diameter (SD) and branch diameter (BD) are closely related traits, and genetic clarification of SD and BD is crucial for soybean breeding. Methods: SD and BD were genetically analyzed by a population of 363 RIL derived from the cross between Zhongdou41 (ZD41) and ZYD02878 using restricted two-stage multi-locus genome-wide association, inclusive composite interval mapping, and three-variance component multi-locus random SNP effect mixed linear modeling. Then candidate genes of major QTLs were selected and genetic selection model of SD and BD were constructed respectively. Results and discussion: The results showed that SD and BD were significantly correlated (r = 0.74, P < 0.001). A total of 93 and 84 unique quantitative trait loci (QTL) were detected for SD and BD, respectively by three different methods. There were two and ten major QTLs for SD and BD, respectively, with phenotypic variance explained (PVE) by more than 10%. Within these loci, seven genes involved in the regulation of phytohormones (IAA and GA) and cell proliferation and showing extensive expression of shoot apical meristematic genes were selected as candidate genes. Genomic selection (GS) analysis showed that the trait-associated markers identified in this study reached 0.47-0.73 in terms of prediction accuracy, which was enhanced by 6.56-23.69% compared with genome-wide markers. These results clarify the genetic basis of SD and BD, which laid solid foundation in regulation gene cloning, and GS models constructed could be potentially applied in future breeding programs.
ABSTRACT
Stem diameter is a critical phenotypic parameter for maize, integral to yield prediction and lodging resistance assessment. Traditionally, the quantification of this parameter through manual measurement has been the norm, notwithstanding its tedious and laborious nature. To address these challenges, this study introduces a non-invasive field-based system utilizing depth information from RGB-D cameras to measure maize stem diameter. This technology offers a practical solution for conducting rapid and non-destructive phenotyping. Firstly, RGB images, depth images, and 3D point clouds of maize stems were captured using an RGB-D camera, and precise alignment between the RGB and depth images was achieved. Subsequently, the contours of maize stems were delineated using 2D image processing techniques, followed by the extraction of the stem's skeletal structure employing a thinning-based skeletonization algorithm. Furthermore, within the areas of interest on the maize stems, horizontal lines were constructed using points on the skeletal structure, resulting in 2D pixel coordinates at the intersections of these horizontal lines with the maize stem contours. Subsequently, a back-projection transformation from 2D pixel coordinates to 3D world coordinates was achieved by combining the depth data with the camera's intrinsic parameters. The 3D world coordinates were then precisely mapped onto the 3D point cloud using rigid transformation techniques. Finally, the maize stem diameter was sensed and determined by calculating the Euclidean distance between pairs of 3D world coordinate points. The method demonstrated a Mean Absolute Percentage Error (MAPE) of 3.01%, a Mean Absolute Error (MAE) of 0.75 mm, a Root Mean Square Error (RMSE) of 1.07 mm, and a coefficient of determination (R²) of 0.96, ensuring accurate measurement of maize stem diameter. This research not only provides a new method of precise and efficient crop phenotypic analysis but also offers theoretical knowledge for the advancement of precision agriculture.
ABSTRACT
Corn (Zea mays) silage is an important and popular feed for dairy production in the Amazon region, so it is necessary to evaluate the agronomic performance of forage varieties of corn for cattle feeding in Amazonas. For this purpose, three corn varieties were evaluated (variety 1: Yellow Starchy Corn, variety 2: Chuska INIA 617, and variety 3: DOW 2B710), with two planting densities (density 1: 30 × 80 cm and density 2: 35 × 75 cm) and two fertilization conditions: with fertilization (F1) and without fertilization (F2). The parameters evaluated were plant height, number of leaves, leaf length and width, stem diameter, fresh forage, and dry matter. Student t-tests, correlation analysis of variables, and principal component analysis using R software version 4.1.3 were used for data analysis. The results indicated that variety 2 obtained the best values for the variables leaf width (12.33 cm) and stem diameter (3.25 cm), fresh forage (17.77 kg/m2), and dry matter (4.8 kg/m2), which would explain the directly proportional correlation found between leaf width and stem diameter with fresh forage and dry matter. The principal component analysis showed constant height and leaf length increases, and the best-evaluated parameters were associated with applying fertilizer. The variety that showed the best agronomic performance under Chachapoyas conditions was Chuska INIA 617, emerging as a potential feed for cattle.
ABSTRACT
INTRODUCTION: The rate of revision TKA and thus the use of hinged implants (HI) steadily rises. Aseptic loosening lies on the top of the failure patterns. However, no evidence exists until now based on national scale high-caseloads that analyzes the impact of cemented HI stem-design on aseptic survival rates. METHODS: Data on aseptic HI-revisions with full-cemented tibia-stems were conducted from the German Arthroplasty Registry. Cases were divided in primary HI (PHI) and HI used in revision operations (RHI). Endpoint was a new revision following either a PHI or an RHI. The impact of stem conicity (conical vs. cylindrical), diameter (≤ 13 mm vs. > 13 mm), length (≤ 90 mm vs. > 90 mm) and offset on the 6-Year-Cumulative-Aseptic-Revision-Rate (6Y-CARR) was estimated via Kaplan-Meier curve and compared between groups via Log-Rank-Tests. RESULTS: 3953 PHI and 2032 RHI fulfilled inclusion-criteria. Stem conicity had no impact on 6Y-CARR (p = 0.08 and p = 0.8). Diameter > 13 mm hat an impact on PHI (p = 0.05) with lower 6Y-CARR but not on RHI (p = 0.2). Length > 90 mm showed significantly worst 6Y-CARR in PHI (p = 0.0001) but not in RHI (p = 0.3). Offset-stems showed significantly better 6Y-CARR in PHI (p = 0.04), but not in RHI (p = 0.7). CONCLUSION: There was no significant impact of the cemented tibia-stem conicity on 6Y-CARR, neither in PHI nor in RHI. The effect of length, diameter and offset on the 6Y-CARR observed in the PHI, was not detectable in the more complex RHI-cases reflecting its limited clinical relevance by itself in more multifactorial backgrounds. Therefore, results must be interpreted with caution due to considerable system-effects and different utilization-scenarios.
Subject(s)
Arthroplasty, Replacement, Knee , Knee Prosthesis , Prosthesis Design , Prosthesis Failure , Registries , Reoperation , Humans , Arthroplasty, Replacement, Knee/methods , Arthroplasty, Replacement, Knee/instrumentation , Germany/epidemiology , Reoperation/statistics & numerical data , Male , Aged , Female , Tibia/surgery , Middle Aged , Bone CementsABSTRACT
A specific, robust threshold for drought-induced tree mortality is needed to improve the prediction of forest dieback. Here, we tested the relevance of continuous measurements of stem diameter variations for identifying such a threshold, their relationship with hydraulic and cellular damage mechanisms, and the influence of growth conditions on these relationships. Poplar saplings were grown under well-watered, water-limited, or light-limited conditions and then submitted to a drought followed by rewatering. Stem diameter was continuously measured to investigate two parameters: the percentage loss of diameter (PLD) and the percentage of diameter recovery (DR) following rewatering. Water potentials, stomatal conductance, embolism, and electrolyte leakage were also measured, and light microscopy allowed investigating cell collapse induced by drought. The water release observed through loss of diameter occurred throughout the drought, regardless of growth conditions. Poplars did not recover from drought when PLD reached a threshold and this differed according to growth conditions but remained linked to cell resistance to damage and collapse. Our findings shed new light on the mechanisms of drought-induced tree mortality and indicate that PLD could be a relevant indicator of drought-induced tree mortality, regardless of the growth conditions.
Subject(s)
Plant Leaves , Populus , Droughts , Xylem , Plant Transpiration , Water , TreesABSTRACT
Frost resistance is the major factor affecting the distribution of plant species at high latitude and elevation. The main effects of freeze-thaw cycles are damage to living cells and formation of gas embolism in tree xylem vessels. Lethal intracellular freezing can be prevented in living cells by two mechanisms, such as dehydration and deep supercooling. We developed a multiphysics numerical model coupling water flow, heat transfer and phase change, considering different cell types in plant tissues, to study the dynamics and extent of cell dehydration, xylem pressure changes and stem diameter changes in response to freezing and thawing. Results were validated using experimental data for stem diameter changes of walnut trees (Juglans regia). The effect of cell mechanical properties was found to be negligible as long as the intracellular tension developed during dehydration was sufficiently low compared with the ice-induced cryostatic suction. The model was finally used to explore the coupled effects of relevant physiological parameters (initial water and sugar content) and environmental conditions (air temperature variations) on the dynamics and extent of dehydration. It revealed configurations where cell dehydration could be sufficient to protect cells from intracellular freezing, and situations where supercooling was necessary. This model, freely available with this paper, could easily be extended to explore different anatomical structures, different species and more complex physical processes.
Subject(s)
Juglans , Trees , Freezing , Trees/physiology , Dehydration , Water/physiology , Temperature , Juglans/physiology , Plant StemsABSTRACT
Mechanical and chemical methods are widely used to control woody plant encroachment in many African countries. However, very little is known about the effectiveness of these control methods among woody species of different ages. We conducted a field experiment to determine the effects of different tree removal treatments (10%, 20%, 50%, 75% and 100%) and herbicide application (Picloram; 6 mL L-1) on the resprouting ability and vigour of 12 woody plant species. We examined 20 plots (30 m × 30 m) that were each subjected to tree removal, followed by herbicide application on half of the stems for each plot. All the tree species in this study resprouted after cutting. The applied concentration of herbicide significantly reduced the shoot production for Ehretia rigida, Vachellia robusta and Ziziphus mucronata, with a marginal effect for Dichrostachys cinerea. The diameter of stems was an important factor in determining resprouting ability, with shoot production decreasing with increasing stem diameter. However, stem diameter did not affect shoot length and diameter for all species. We found that woody plants are more likely to resprout and survive as juveniles than as adults after cutting and that herbicide only affected four of the twelve species at a concentration of 6 mL L-1. Thus, testing the amount of Picloram needed to kill certain woody species may be of importance for land users in southern African savannas.
ABSTRACT
As part of the plant water-use process, plant nocturnal sap flow (Q n) has been demonstrated to have important ecophysiological significance to compensate for water loss. The purpose of this study was to explore nocturnal water-use strategies to fill the knowledge gap in mangroves, by measuring three species co-occurring in a subtropical estuary. Sap flow was monitored over an entire year using thermal diffusive probes. Stem diameter and leaf-level gas exchange were measured in summer. The data were used to explore the different nocturnal water balance maintaining mechanisms among species. The Q n existed persistently and contributed markedly over 5.5%~24.0% of the daily sap flow (Q) across species, which was associated with two processes, nocturnal transpiration (E n) and nocturnal stem water refilling (R n). We found that the stem recharge of the Kandelia obovata and Aegiceras corniculatum occurred mainly after sunset and that the high salinity environment drove higher Q n while stem recharge of the Avicennia marina mainly occurred in the daytime and the high salinity environment inhibited the Q n. The diversity of stem recharge patterns and response to sap flow to high salinity conditions were the main reasons for the differences in Q n/Q among species. For Kandelia obovata and Aegiceras corniculatum, R n was the main contributor to Q n, which was driven by the demands of stem water refilling after diurnal water depletion and high salt environment. Both of the species have a strict control over the stomata to reduce water loss at night. In contrast, Avicennia marina maintained a low Q n, driven by vapor pressure deficit, and the Q n mainly used for E n, which adapts to high salinity conditions by limiting water dissipation at night. We conclude that the diverse ways Q n properties act as water-compensating strategies among the co-occurring mangrove species might help the trees to overcoming water scarcity.
ABSTRACT
Stored water in inner tissues influences the plant water economy, which might be particularly relevant for trees facing increasing dry conditions due to climate change. We studied the water storage in the inner bark and the sapwood of Araucaria araucana (Molina) K. Koch. This species has an extremely thick inner bark and thus it can be used as a model system to assess the impact of internal water storage on plant water balance. Specifically, we analyzed the water circulation pathways in and out of the elastic water storages by using simultaneously frequency domain moisture sensors and dendrometers inserted in the inner bark and in the sapwood, and sap flow determinations during the dry season. The daily patterns of water content and expansion and contraction of the stem tissues were similar to the sap flow pattern. The whole-stem water content and diameter increased in the morning and decreased in the afternoon, contrary to the typical pattern observed in most tree species. An osmotic gradient favoring the water influx from sapwood to inner bark was observed in the morning. There were no lags in the onset of sap flow between different stem heights at the time that recharge of reservoirs occurred. Sap flow at 6 m height was higher than basal sap flow in the afternoon, when the sapwood water content started to decline followed by the water content of the inner bark. Inner bark and sapwood contributed 5-11% to total daily transpiration, allowing the maintenance of high water potentials in the dry season. Our results suggest that the stored water in the stems, the atypical dynamic of recharge and discharge of water from reservoirs and the high tissue capacitance may make an important contribution to the survival of A. araucana during drought periods by maintaining the water balance.
Subject(s)
Araucaria araucana , Water , Water/metabolism , Droughts , Plant Bark/metabolism , Plant Transpiration , Circadian Rhythm , Trees/metabolism , Plant Stems/metabolismABSTRACT
Lodging reduces grain yield in cereal crops. The height, diameter and strength of stem are crucial for lodging resistance, grain yield, and photosynthate transport in barley. Understanding the genetic basis of stem benefits barley breeding. Here, we evaluated 13 stem related traits after 28 days of heading in a barley DH population in two consecutive years. Significant phenotypic correlations between lodging index (LI) and other stem traits were observed. Three mapping methods using the experimental data and the BLUP data, detected 27 stable and major QTLs, and 22 QTL clustered regions. Many QTLs were consistent with previously reported traits for grain filling rate, internodes, panicle and lodging resistance. Further, candidate genes were predicted for stable and major QTLs and were associated with plant development and adverse stress in the transition from vegetative stage to reproductive stage. This study provided potential genetic basis and new information for exploring barley stem morphology, and laid a foundation for map-based cloning and further fine mapping of these QTLs.
ABSTRACT
The influence of different mulching measures on soil moisture, soil temperature, and crop growth was investigated during the jujube growing season in rain-fed jujube orchards using micro-plot experiments. The mulching treatments included clean tillage (CT, control treatment), jujube branches mulching (JBM), and white clover planting (WCP). The results revealed that: (1) The average soil moisture content of JBM was greater than that of CT by 3.76% and 2.34%, respectively, during the 2013 and 2014 jujube growth periods, and its soil water deficit was minimal in each soil layer from 0 to 70 cm. WCP had the greatest soil water deficit. The average soil moisture content of the 0−70 cm soil layer in WCP was 3.88% and 5.55% lower than that in CT during the 2013 and 2014 jujube growth seasons, respectively (p < 0.05). (2) JBM had the highest annual average soil moisture content in each soil layer from 0 to 70 cm, followed by CT, while WCP had the lowest. White clover and jujube competed for water in the 20−40 cm soil layer, and JBM had the lowest variation in soil moisture. (3) Mulching with jujube branches and planting white clover could both control the temperature of the 0−25 cm soil layer and narrow the daily temperature range, with JBM being the least affected by air temperature. (4) Jujube's leaf area index and stem diameter increase in JBM were both significantly greater than in CT and WCP. In conclusion, using pruned jujube branches as surface mulch is appropriate for rain-fed jujube orchards because it can preserve soil moisture, regulate soil temperature, and promote jujube growth.
ABSTRACT
The stem diameter, an important agronomic trait, affects cucumber growth and yield. However, no genes responsible for cucumber stem diameter have been identified yet. In this study, the stem diameter of 88 cucumber core germplasms were measured in spring 2020, autumn 2020 and autumn 2021, and a genome-wide association study (GWAS) was carried out based on the gene sequence and stem diameter of core germplasms. A total of eight loci (gSD1.1, gSD2.1, gSD3.1, gSD3.2, gSD4.1, gSD5.1, gSD5.2, and gSD6.1) significantly associated with cucumber stem diameter were detected. Of these, five loci (gSD1.1, gSD2.1, gSD3.1, gSD5.2, and gSD6.1) were repeatedly detected in two or more seasons and were considered as robust and reliable loci. Based on the linkage disequilibrium sequences of the associated SNP loci, 37 genes were selected. By further investigating the five loci via analyzing Arabidopsis homologous genes and gene haplotypes, five genes (CsaV3_1G028310, CsaV3_2G006960, CsaV3_3G009560, CsaV3_5G031320, and CsaV3_6G031260) showed variations in amino acid sequence between thick stem lines and thin stem lines. Expression pattern analyses of these genes also showed a significant difference between thick stem and thin stem lines. This study laid the foundation for gene cloning and molecular mechanism study of cucumber stem development.
Subject(s)
Cucumis sativus , Glycogen Storage Disease Type II , Cucumis sativus/genetics , Genome-Wide Association Study , Linkage Disequilibrium , PhenotypeABSTRACT
Data capturing multiple axes of tree size and shape, such as a tree's stem diameter, height and crown size, underpin a wide range of ecological research-from developing and testing theory on forest structure and dynamics, to estimating forest carbon stocks and their uncertainties, and integrating remote sensing imagery into forest monitoring programmes. However, these data can be surprisingly hard to come by, particularly for certain regions of the world and for specific taxonomic groups, posing a real barrier to progress in these fields. To overcome this challenge, we developed the Tallo database, a collection of 498,838 georeferenced and taxonomically standardized records of individual trees for which stem diameter, height and/or crown radius have been measured. These data were collected at 61,856 globally distributed sites, spanning all major forested and non-forested biomes. The majority of trees in the database are identified to species (88%), and collectively Tallo includes data for 5163 species distributed across 1453 genera and 187 plant families. The database is publicly archived under a CC-BY 4.0 licence and can be access from: https://doi.org/10.5281/zenodo.6637599. To demonstrate its value, here we present three case studies that highlight how the Tallo database can be used to address a range of theoretical and applied questions in ecology-from testing the predictions of metabolic scaling theory, to exploring the limits of tree allometric plasticity along environmental gradients and modelling global variation in maximum attainable tree height. In doing so, we provide a key resource for field ecologists, remote sensing researchers and the modelling community working together to better understand the role that trees play in regulating the terrestrial carbon cycle.
Subject(s)
Forests , Trees , Biomass , Carbon/metabolism , Carbon Cycle , Ecosystem , Trees/physiologyABSTRACT
BACKGROUND: The number of banana plants is closely related to banana yield. The diameter and height of the pseudo-stem are important morphological parameters of banana plants, which can reflect the growth status and vitality. To address the problems of high labor intensity and subjectivity in traditional measurement methods, a fast measurement method for banana plant count, pseudo-stem diameter, and height based on terrestrial laser scanning (TLS) was proposed. RESULTS: First, during the nutritional growth period of banana, three-dimensional (3D) point cloud data of two measured fields were obtained by TLS. Second, the point cloud data was preprocessed. And the single plant segmentation of the canopy closed banana plant point cloud was realized furtherly. Finally, the number of banana plants was obtained by counting the number of pseudo-stems, and the diameter of pseudo-stems was measured using a cylindrical segmentation algorithm. A sliding window recognition method was proposed to determine the junction position between leaves and pseudo-stems, and the height of the pseudo-stems was measured. Compared with the measured value of artificial point cloud, when counting the number of banana plants, the precision,recall and percentage error of field 1 were 93.51%, 94.02%, and 0.54% respectively; the precision,recall and percentage error of field 2 were 96.34%, 92.00%, and 4.5% respectively; In the measurement of pseudo-stem diameter and height of banana, the root mean square error (RMSE) of pseudo-stem diameter and height of banana plant in field 1 were 0.38 cm and 0.2014 m respectively, and the mean absolute percentage error (MAPE) were 1.30% and 5.11% respectively; the RMSE of pseudo-stem diameter and height of banana plant in field 2 were 0.39 cm and 0.2788 m respectively, and the MAPE were 1.04% and 9.40% respectively. CONCLUSION: The results show that the method proposed in this paper is suitable for the field measurement of banana count, pseudo-stem diameter, and height and can provide a fast field measurement method for banana plantation management.