ABSTRACT
OBJECTIVE: To evaluate if the cellularity of Hybrid Capture samples (Digene, São Paulo, Brazil) influences the results of HPV-DNA Hybrid Capture tests in men. STUDY DESIGN: We harvested material from penile scrapings for the Hybrid Capture HPV test. This material was then used to make cytologic smears, which we used to evaluate for the presence of nonnucleated squamous cells, nucleated squamous cells and glandular cells. The cellularity of nucleated squamous cells was classified as absent, low, moderate or high. Subsequently, we performed the Hybrid Capture test to identify the low and high risk of HPV and compared these results with the cytologic findings. We used the Fisher and odds ratio tests at CI of 95% to determine statistical significance. RESULTS: Of the 88 tests performed, 65 (74.0%) were negative for HPV-DNA and 23 (26.0%) were positive. Nucleated and nonnucleated squamous cells were absent on nine slides, all of which tested negative for HPV. When only nonnudcleated squamous cells were found, 20% of the cases were positive for HPV-DNA (p < 0.0001; OR = 26.185). The presence of nucleated squamous cells correlated with 33% HPV-DNA positivity (p < 0.0001, OR = 49.05). CONCLUSION: Assessing the presence of non-nucleated and nucleated squamous cells on cytologic smears prior to performing an HPV-DNA test is a useful tool for quality control in penile samples.
Subject(s)
DNA, Viral/analysis , Nucleic Acid Hybridization/methods , Papillomaviridae/genetics , Papillomavirus Infections/diagnosis , Penile Diseases/diagnosis , Cell Nucleus/pathology , Cell Nucleus/virology , Cytodiagnosis/methods , DNA Probes, HPV/genetics , Epithelial Cells/pathology , Epithelial Cells/virology , Humans , Male , Papillomavirus Infections/virology , Penile Diseases/virologyABSTRACT
BACKGROUND: The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions. AIM: To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. MATERIAL AND METHODS: A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism. RESULTS: All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76% of women. Fourteen percent had an infection with multiple subtypes and in 10%, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53. CONCLUSIONS: There is a high rate of infection with HPV with a high oncogenic risk among these women.
Subject(s)
DNA, Viral/isolation & purification , Papillomaviridae/isolation & purification , Precancerous Conditions/virology , Uterine Cervical Dysplasia/virology , Uterine Cervical Neoplasms/virology , Vaginal Smears , Adolescent , Adult , Aged , Amplified Fragment Length Polymorphism Analysis , DNA Probes, HPV/genetics , DNA Probes, HPV/isolation & purification , Female , Genotype , Humans , Middle Aged , Papillomaviridae/classification , Papillomaviridae/genetics , Papillomavirus Infections/complications , Papillomavirus Infections/genetics , Polymorphism, Restriction Fragment Length , Severity of Illness IndexABSTRACT
Background: The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions. Aim: To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. Material and methods: A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism. Results: All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76 percent of women. Fourteen percent had an infection with multiple subtypes and in 10 percent, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53. Conclusions: There is a high rate of infection with HPV with a high oncogenic risk among these women.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Middle Aged , Uterine Cervical Dysplasia/virology , DNA, Viral/isolation & purification , Papillomaviridae/isolation & purification , Precancerous Conditions/virology , Uterine Cervical Dysplasia/virology , Uterine Cervical Neoplasms/virology , Vaginal Smears , Amplified Fragment Length Polymorphism Analysis , DNA Probes, HPV/genetics , DNA Probes, HPV/isolation & purification , Genotype , Papillomaviridae/classification , Papillomaviridae/genetics , Papillomavirus Infections/complications , Papillomavirus Infections/genetics , Polymorphism, Restriction Fragment Length , Severity of Illness IndexABSTRACT
El factor inmunológico además del tipo viral, ha sido involucreado en la cronicidad y evolución de las lesiones del TGI por HPV. En este trabajo hemos investigado la presencia de secuencias de DNA de HPV en células mononucleares de sangre periférica (CMSP) de mujeres sanas y con lesiones de TGI astribuídas a infecciones por HPV
Subject(s)
Humans , Female , Adult , DNA Probes, HPV/genetics , Papillomaviridae/enzymology , Papillomaviridae/genetics , Papillomaviridae/immunology , Papillomaviridae/isolation & purification , Papillomavirus Infections/diagnosisABSTRACT
El factor inmunológico además del tipo viral, ha sido involucreado en la cronicidad y evolución de las lesiones del TGI por HPV. En este trabajo hemos investigado la presencia de secuencias de DNA de HPV en células mononucleares de sangre periférica (CMSP) de mujeres sanas y con lesiones de TGI astribuídas a infecciones por HPV(AU)
Subject(s)
Humans , Female , Adult , /diagnosis , Human Papillomavirus Viruses/enzymology , Human Papillomavirus Viruses/isolation & purification , Human Papillomavirus Viruses/genetics , Human Papillomavirus Viruses/immunology , DNA Probes, HPV/geneticsABSTRACT
El gen p53 regula el ingreso de las células a la fase S del ciclo celular mediante la síntesis de una proteína efectora. La mutación de este gen se relacionaría con la pérdida de la capacidad supresora y la concomitante progresión tumoral. La génesis del carcinoma de cérvix podría vincularse a la mutación del gen p53 por acción del ADN viral (HPV). Realizamos la inmunomarcación de la proteína p53 en 88 biopsias de cérvix. De éstas, 10 correspondieron a cuello normal, 41 a lesiones intraepiteliales de bajo grado, 24 a lesiones intraepiteliales de alto grado y 13 a carcinomas invasores. La positividad fue del 10 por ciento, 58,5 por ciento, 70,8 por ciento y 92,3 por ciento respectivamente. Se evaluó también: atipia coilocitótica, reacción inflamatoria, localización e intensidad de la inmunomarcación, tipo de carcinoma invasor, etc. En este informe queda demostrado que el mayor grado de lesión correlaciona con mayor expresión de proteína p53 anómala o mutada
Subject(s)
Humans , Female , DNA Probes, HPV/genetics , Genes, p53/physiology , Immunohistochemistry/statistics & numerical data , Papillomavirus Infections/complications , Tumor Suppressor Protein p53/adverse effects , Uterine Cervical Neoplasms/etiology , Carcinoma/pathology , Condylomata Acuminata/virology , DNA Probes, HPV/adverse effects , Papillomaviridae/pathogenicity , Tumor Suppressor Protein p53/genetics , Uterine Cervical Neoplasms/genetics , Uterine Cervical Neoplasms/virologyABSTRACT
El gen p53 regula el ingreso de las células a la fase S del ciclo celular mediante la síntesis de una proteína efectora. La mutación de este gen se relacionaría con la pérdida de la capacidad supresora y la concomitante progresión tumoral. La génesis del carcinoma de cérvix podría vincularse a la mutación del gen p53 por acción del ADN viral (HPV). Realizamos la inmunomarcación de la proteína p53 en 88 biopsias de cérvix. De éstas, 10 correspondieron a cuello normal, 41 a lesiones intraepiteliales de bajo grado, 24 a lesiones intraepiteliales de alto grado y 13 a carcinomas invasores. La positividad fue del 10 por ciento, 58,5 por ciento, 70,8 por ciento y 92,3 por ciento respectivamente. Se evaluó también: atipia coilocitótica, reacción inflamatoria, localización e intensidad de la inmunomarcación, tipo de carcinoma invasor, etc. En este informe queda demostrado que el mayor grado de lesión correlaciona con mayor expresión de proteína p53 anómala o mutada (AU)