ABSTRACT
Crossover recombination during meiosis is accompanied by a dramatic chromosome reorganization. In Saccharomyces cerevisiae, the onset of meiotic recombination by the Spo11 transesterase leads to stable pairwise associations between previously unassociated homologous centromeres followed by the intimate alignment of homologous axes via synaptonemal complex (SC) assembly. However, the molecular relationship between recombination and global meiotic chromosome reorganization remains poorly understood. In budding yeast, one question is why SC assembly initiates earliest at centromere regions while the DNA double strand breaks (DSBs) that initiate recombination occur genome-wide. We targeted the site-specific HO endonuclease to various positions on S. cerevisiae's longest chromosome in order to ask whether a meiotic DSB's proximity to the centromere influences its capacity to promote homologous centromere pairing and SC assembly. We show that repair of an HO-mediated DSB does not promote homologous centromere pairing nor any extent of SC assembly in spo11 meiotic nuclei, regardless of its proximity to the centromere. DSBs induced en masse by phleomycin exposure likewise do not promote homologous centromere pairing nor robust SC assembly. Interestingly, in contrast to Spo11, HO-initiated interhomolog recombination is not affected by loss of the meiotic kinase, Mek1, and is not constrained to use the meiosis-specific Dmc1 recombinase. These results strengthen the previously proposed idea that (at least some) Spo11 DSBs may be specialized in activating mechanisms that both 1) reinforce homologous chromosome alignment via homologous centromere pairing and SC assembly, and 2) establish Dmc1 as the primary strand exchange enzyme.
Subject(s)
Cell Cycle Proteins/physiology , DNA-Binding Proteins/physiology , Deoxyribonucleases, Type II Site-Specific/physiology , Saccharomyces cerevisiae Proteins/physiology , Saccharomyces cerevisiae/physiology , Centromere , Endodeoxyribonucleases/physiology , Meiosis , Recombination, GeneticABSTRACT
Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction. However, large DNA fragments can only be assembled by alternating use of two or more type IIS restriction enzymes in a multi-step approach. Here, we present MetClo, a DNA assembly method that uses only a single type IIS restriction enzyme for hierarchical DNA assembly. The method is based on in vivo methylation-mediated on/off switching of type IIS restriction enzyme recognition sites that overlap with site-specific methylase recognition sequences. We have developed practical MetClo systems for the type IIS enzymes BsaI, BpiI and LguI, and demonstrated hierarchical assembly of large DNA fragments up to 218 kb. The MetClo approach substantially reduces the need to remove internal restriction sites from components to be assembled. The use of a single type IIS enzyme throughout the different stages of DNA assembly allows novel and powerful design schemes for rapid large-scale hierarchical DNA assembly. The BsaI-based MetClo system is backward-compatible with component libraries of most of the existing type IIS restriction enzyme-based assembly systems, and has potential to become a standard for modular DNA assembly.
Subject(s)
Cloning, Molecular/methods , DNA Modification Methylases/physiology , Deoxyribonucleases, Type II Site-Specific/physiology , Base Sequence , DNA Ligases/metabolism , DNA Restriction Enzymes/classification , DNA Restriction Enzymes/physiology , DNA, Recombinant/biosynthesis , DNA, Recombinant/genetics , Escherichia coli , Gene Library , Synthetic Biology/methodsABSTRACT
The cholesteryl ester transfer protein (CETP) plays a crucial role in high-density lipoprotein (HDL) metabolism. Genetic variants that alter CETP concentration may cause significant alterations in HDL-cholesterol (HDL-C) concentration. In this case-control study, we analyzed the genotype frequencies of CETP Taq1B polymorphisms in coronary artery disease patients (CAD; n=210) and controls (n=100). We analyzed the role of the CETP Taq1B variant in severity of CAD, and its association with plasma lipids and CETP concentration. DNA was extracted from 310 patients undergoing coronary angiography. The Taq1B polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (RFLP) analysis. Lipid concentrations were measured by an auto analyzer and CETP level by a commercial enzyme-linked immunosorbent assay (ELISA) kit. In our study population, the B2 allele frequency was higher in control subjects than patients with single, double or triple vessel disease. B2B2 genotype carriers had a significantly higher high-density lipoprotein cholesterol (HDL-C) concentration than those with the B1B1 genotype in controls (51.93±9.47versus 45.34±9.93; p<0.05) and in CAD patients (45.52±10.81 versus 40.38±9.12; p<0.05). B2B2 genotype carriers had a significantly lower CETP concentration than those with the B1B1 genotype in controls (1.39±0.58 versus 1.88±0.83; p< 0.05) and in CAD patients (2.04±1.39versus 2.81±1.68; p< 0.05). Our data suggest that the B2 allele is associated with higher concentrations of HDL-C and lower concentrations of CETP, which confer a protective effect on coronary artery disease.
Subject(s)
Cholesterol Ester Transfer Proteins/genetics , Coronary Artery Disease/genetics , DNA Restriction Enzymes/genetics , Deoxyribonucleases, Type II Site-Specific/genetics , Polymorphism, Genetic/genetics , Severity of Illness Index , Aged , Case-Control Studies , Cholesterol Ester Transfer Proteins/physiology , Cholesterol, HDL/metabolism , Coronary Angiography , Coronary Artery Disease/epidemiology , Coronary Artery Disease/physiopathology , DNA Restriction Enzymes/physiology , Deoxyribonucleases, Type II Site-Specific/physiology , Female , Gene Frequency/genetics , Genotype , Humans , Lipid Metabolism/physiology , Lipoproteins, HDL/metabolism , Male , Middle Aged , Pilot Projects , Turkey/epidemiologyABSTRACT
Recent advances in the burgeoning field of genome engineering are accelerating the realization of personalized therapeutics for cardiovascular disease. In the postgenomic era, sequence-specific gene-editing tools enable the functional analysis of genetic alterations implicated in disease. In partnership with high-throughput model systems, efficient gene manipulation provides an increasingly powerful toolkit to study phenotypes associated with patient-specific genetic defects. Herein, this review emphasizes the latest developments in genome engineering and how applications within the field are transforming our understanding of personalized medicine with an emphasis on cardiovascular diseases.
Subject(s)
Bacterial Proteins/physiology , Cardiovascular Diseases/genetics , Deoxyribonucleases, Type II Site-Specific/physiology , Deoxyribonucleases/physiology , Genetic Engineering/methods , Genomics , Induced Pluripotent Stem Cells/cytology , Mutagenesis, Site-Directed/methods , Precision Medicine/trends , Xanthomonas/enzymology , Animals , Bacterial Proteins/genetics , Binding Sites , Cardiovascular Diseases/therapy , Cell Differentiation , Cells, Cultured/cytology , Cells, Cultured/transplantation , DNA/genetics , DNA/metabolism , Deoxyribonucleases/genetics , Deoxyribonucleases, Type II Site-Specific/genetics , Forecasting , Genes, Reporter , Genetic Engineering/trends , Humans , Induced Pluripotent Stem Cells/transplantation , Models, Cardiovascular , Models, Genetic , Protein Structure, Tertiary , Recombinant Fusion Proteins/physiology , Recombinational DNA Repair , Substrate Specificity , Xanthomonas axonopodis/enzymology , Zebrafish/geneticsABSTRACT
Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci. However, a lack of consensus exists regarding a perfect genomic safe harbour (GSH) that would allow transgenes to be stably and reliably expressed without adversely affecting endogenous gene structure and function. Ribosomal DNA (rDNA) has many advantages as a GSH, but efficient means to target integration to this locus are currently lacking. We tested whether lentivirus vector integration can be directed to rDNA by using fusion proteins consisting of the Human Immunodeficiency Virus 1 (HIV-1) integrase (IN) and the homing endonuclease I-PpoI, which has natural cleavage sites in the rDNA. A point mutation (N119A) was introduced into I-PpoI to abolish unwanted DNA cleavage by the endonuclease. The vector-incorporated IN-I-PpoIN119A fusion protein targeted integration into rDNA significantly more than unmodified lentivirus vectors, with an efficiency of 2.7%. Our findings show that IN-fusion proteins can be used to modify the integration pattern of lentivirus vectors, and to package site-specific DNA-recognizing proteins into vectors to obtain safer transgene integration.
Subject(s)
DNA, Ribosomal/genetics , Deoxyribonucleases, Type II Site-Specific/genetics , HIV Integrase/genetics , Mutagenesis, Insertional/methods , Recombinant Fusion Proteins/genetics , Cloning, Molecular , DNA Breaks, Double-Stranded , Deoxyribonucleases, Type II Site-Specific/biosynthesis , Deoxyribonucleases, Type II Site-Specific/physiology , Genetic Vectors , HEK293 Cells , HIV Integrase/biosynthesis , HIV Integrase/physiology , HIV-1/enzymology , HeLa Cells , Humans , Lentivirus/genetics , Physarum polycephalum/enzymology , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/physiology , Transduction, GeneticABSTRACT
The FokI endonuclease is a monomeric protein with discrete DNA-recognition and catalytic domains. The latter has only one active site so, to cut both strands, the catalytic domains from two monomers associate to form a dimer. The dimer involving a monomer at the recognition site and another from free solution is less stable than that from two proteins tethered to the same DNA. FokI thus cleaves DNA with two sites better than one-site DNA. The two sites can be immediately adjacent, but they can alternatively be many hundreds of base pairs apart, in either inverted or repeated orientations. The catalytic domain of FokI is often a component of zinc finger nucleases. Typically, the zinc finger domains of two such nucleases are designed to recognize two neighbouring DNA sequences, with the objective of cutting the DNA exclusively between the target sequences. However, this strategy fails to take account of the fact that the catalytic domains of FokI can dimerize across distant sites or even at a solitary site. Additional copies of either target sequence elsewhere in the chromosome must elicit off-target cleavages.
Subject(s)
DNA/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Deoxyribonucleases/metabolism , Animals , Base Sequence , Catalytic Domain/genetics , Deoxyribonucleases, Type II Site-Specific/physiology , Enzyme Activation/physiology , Humans , Models, Biological , Zinc Fingers/physiologyABSTRACT
The endonucleases from the Type IIB restriction-modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex. The fact that these enzymes cut DNA at specific locations mark them as Type II systems, as opposed to the Type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most Type IIB restriction-modification systems have more in common with Type I than with other Type II systems. Our current knowledge of the Type IIB systems is reviewed in the present paper.
Subject(s)
Deoxyribonucleases, Type II Site-Specific/physiology , Base Sequence , Binding Sites , DNA/chemistry , DNA/metabolism , DNA Restriction Enzymes/chemistry , DNA Restriction Enzymes/classification , DNA Restriction Enzymes/metabolism , DNA Restriction Enzymes/physiology , Deoxyribonucleases, Type II Site-Specific/chemistry , Deoxyribonucleases, Type II Site-Specific/classification , Deoxyribonucleases, Type II Site-Specific/metabolism , Protein Binding/physiology , Substrate SpecificityABSTRACT
Homing endonucleases (HE) are double-stranded DNAses that target large recognition sites (12-40 bp). HE-encoding sequences are usually embedded in either introns or inteins. Their recognition sites are extremely rare, with none or only a few of these sites present in a mammalian-sized genome. However, these enzymes, unlike standard restriction endonucleases, tolerate some sequence degeneracy within their recognition sequence. Several members of this enzyme family have been used as templates to engineer tools to cleave DNA sequences that differ from their original wild-type targets. These custom HEs can be used to stimulate double-strand break homologous recombination in cells, to induce the repair of defective genes with very low toxicity levels. The use of tailored HEs opens up new possibilities for gene therapy in patients with monogenic diseases that can be treated ex vivo. This review provides an overview of recent advances in this field.
Subject(s)
Endodeoxyribonucleases/physiology , Amino Acid Motifs , Animals , DNA Breaks, Double-Stranded , DNA Repair/genetics , DNA Repair/physiology , Deoxyribonucleases, Type II Site-Specific/chemistry , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/metabolism , Deoxyribonucleases, Type II Site-Specific/physiology , Endodeoxyribonucleases/chemistry , Endodeoxyribonucleases/genetics , Endodeoxyribonucleases/metabolism , Genetic Therapy/methods , Humans , Models, Biological , Models, Molecular , Multigene Family/genetics , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae Proteins/physiologyABSTRACT
How are violations of phonological constraints processed in word comprehension? The present article reports the results of an event-related potentials (ERP) study on a phonological constraint of German that disallows identical segments within a syllable or word (CC(i)VC(i)). We examined three types of monosyllabic late positive CCVC words: (a) existing words [see text], (b) wellformed novel words [see text] and component (c) illformed novel words [see text] as instances of Obligatory Contour Principle non-word (OCP) violations. Wellformed and illformed novel words evoked an N400 effect processing in comparison to existing words. In addition, illformed words produced an enhanced late posterior positivity effect compared to wellformed novel words. obligatory contour Our findings support the well-known observation that novel words evoke principle higher costs in lexical integration (reflected by N400 effects). Crucially, modulations of a late positive component (LPC) show that violations of phonological phonotactic constraints influence later stages of cognitive processing even constraints when stimuli have already been detected as non-existing. Thus, the comparison of electrophysiological effects evoked by the two types of non-existing words reveals the stages at which phonologically based structural wellformedness comes into play during word processing.
Subject(s)
Brain/physiology , Deoxyribonucleases, Type II Site-Specific/physiology , Evoked Potentials , Phonetics , Speech Perception/physiology , Acoustic Stimulation , Adult , Cognition/physiology , Electroencephalography , Female , Humans , Language , Male , Speech , Vocabulary , Young AdultABSTRACT
Type II restriction-modification systems are comprised of a restriction endonuclease and methyltransferase. The enzymes are coded by individual genes and recognize the same DNA sequence. Endonuclease makes a double-stranded break in the recognition site, and methyltransferase covalently modifies the DNA bases within the recognition site, thereby down-regulating endonuclease activity. Coordinated action of these enzymes plays a role of primitive immune system and protects bacterial host cell from the invasion of foreign (for example, viral) DNA. However, uncontrolled expression of the restriction-modification system genes can result in the death of bacterial host cell because of the endonuclease cleavage of host DNA. In the present review, the data on the expression regulation of the type II restriction-modification enzymes are discussed.
Subject(s)
DNA Restriction Enzymes/physiology , Bacteria/enzymology , Deoxyribonucleases, Type II Site-Specific/physiology , Gene Expression Regulation, Bacterial , Gene Expression Regulation, Enzymologic , Methyltransferases/physiologyABSTRACT
DNA recognition by proteins is essential for specific expression of genes in a living organism. En route to a target DNA site, a protein will often sample noncognate DNA sites through nonspecific protein-DNA interactions, resulting in a variety of conformationally different binding states. We present here the crystal structure of endonuclease BstYI bound to a noncognate DNA. Surprisingly, the structure reveals the enzyme in a "hemispecific" binding state on the pathway between nonspecific and specific recognition. A single base pair change in the DNA abolishes binding of only one monomer, with the second monomer bound specifically. We show that the enzyme binds essentially as a rigid body, and that one end of the DNA is accommodated loosely in the binding cleft while the other end is held tightly. Another intriguing feature of the structure is Ser172, which has a dual role in establishing nonspecific and specific contacts. Taken together, the structure provides a snapshot of an enzyme in a "paused" intermediate state that may be part of a more general mechanism of scanning DNA.
Subject(s)
DNA/metabolism , Deoxyribonucleases, Type II Site-Specific/physiology , Crystallography, X-Ray , DNA/chemistry , Deoxyribonucleases, Type II Site-Specific/chemistry , Geobacillus stearothermophilus/enzymology , Geobacillus stearothermophilus/genetics , Protein BindingABSTRACT
According to the current paradigm type IIE restriction endonucleases are homodimeric proteins that simultaneously bind to two recognition sites but cleave DNA at only one site per turnover: the other site acts as an allosteric locus, activating the enzyme to cleave DNA at the first. Structural and biochemical analysis of the archetypal type IIE restriction enzyme EcoRII suggests that it has three possible DNA binding interfaces enabling simultaneous binding of three recognition sites. To test if putative synapsis of three binding sites has any functional significance, we have studied EcoRII cleavage of plasmids containing a single, two and three recognition sites under both single turnover and steady state conditions. EcoRII displays distinct reaction patterns on different substrates: (i) it shows virtually no activity on a single site plasmid; (ii) it yields open-circular DNA form nicked at one strand as an obligatory intermediate acting on a two-site plasmid; (iii) it cleaves concertedly both DNA strands at a single site during a single turnover on a three site plasmid to yield linear DNA. Cognate oligonucleotide added in trans increases the reaction velocity and changes the reaction pattern for the EcoRII cleavage of one and two-site plasmids but has little effect on the three-site plasmid. Taken together the data indicate that EcoRII requires simultaneous binding of three rather than two recognition sites in cis to achieve concerted DNA cleavage at a single site. We show that the orthodox type IIP enzyme PspGI which is an isoschisomer of EcoRII, cleaves different plasmid substrates with equal rates. Data provided here indicate that type IIE restriction enzymes EcoRII and NaeI follow different mechanisms. We propose that other type IIE restriction enzymes may employ the mechanism suggested here for EcoRII.
Subject(s)
DNA/metabolism , Deoxyribonucleases, Type II Site-Specific/physiology , Plasmids/genetics , Allosteric Site , Archaea/chemistry , Binding Sites , Deoxyribonucleases, Type II Site-Specific/chemistry , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/metabolism , Hydrolysis , Kinetics , Protein BindingABSTRACT
Activity of Ho, the yeast mating switch endonuclease, is restricted to a narrow time window of the cell cycle. Ho is unstable and despite being a nuclear protein is exported to the cytoplasm for proteasomal degradation. We report here the molecular basis for the highly efficient nuclear import of Ho and the relation between its short half-life and passage through the nucleus. The Ho nuclear import machinery is functionally redundant, being based on two bipartite nuclear localization signals, recognized by four importins of the ribosomal import system. Ho degradation is regulated by the DNA damage response and Ho retained in the cytoplasm is stabilized, implying that Ho acquires its crucial degradation signals in the nucleus. Ho arose by domestication of a fungal VMA1 intein. A comparison of the primary sequences of Ho and fungal VMA1 inteins shows that the Ho nuclear localization signals are highly conserved in all Ho proteins, but are absent from VMA1 inteins. Thus adoption of a highly efficient import strategy occurred very early in the evolution of Ho. This may have been a crucial factor in establishment of homothallism in yeast, and a key event in the rise of the Saccharomyces sensu stricto.
Subject(s)
Deoxyribonucleases, Type II Site-Specific/physiology , Nuclear Localization Signals , Ribosomes/metabolism , Active Transport, Cell Nucleus , Amino Acid Sequence , Biological Transport , Cytoplasm/metabolism , Inteins , Karyopherins/metabolism , Molecular Sequence Data , Mutation , Phylogeny , Plasmids/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae ProteinsABSTRACT
Type II restriction endonuclease Mva1269I recognizes an asymmetric DNA sequence 5'-GAATGCN / -3'/5'-NG / CATTC-3' and cuts top and bottom DNA strands at positions, indicated by the "/" symbol. Most restriction endonucleases require dimerization to cleave both strands of DNA. We found that Mva1269I is a monomer both in solution and upon binding of cognate DNA. Protein fold-recognition analysis revealed that Mva1269I comprises two "PD-(D/E)XK" domains. The N-terminal domain is related to the 5'-GAATTC-3'-specific restriction endonuclease EcoRI, whereas the C-terminal one resembles the nonspecific nuclease domain of restriction endonuclease FokI. Inactivation of the C-terminal catalytic site transformed Mva1269I into a very active bottom strand-nicking enzyme, whereas mutants in the N-terminal domain nicked the top strand, but only at elevated enzyme concentrations. We found that the cleavage of the bottom strand is a prerequisite for the cleavage of the top strand. We suggest that Mva1269I evolved the ability to recognize and to cleave its asymmetrical target by a fusion of an EcoRI-like domain, which incises the bottom strand within the target, and a FokI-like domain that completes the cleavage within the nonspecific region outside the target sequence. Our results have implications for the molecular evolution of restriction endonucleases, as well as for perspectives of engineering new restriction and nicking enzymes with asymmetric target sites.
Subject(s)
Deoxyribonuclease EcoRI/chemistry , Deoxyribonucleases, Type II Site-Specific/chemistry , Micrococcus/metabolism , Amino Acid Sequence , Base Sequence , Calcium/chemistry , Catalytic Domain , Crystallization , DNA/chemistry , Deoxyribonucleases, Type II Site-Specific/metabolism , Deoxyribonucleases, Type II Site-Specific/physiology , Electrophoresis, Polyacrylamide Gel , Evolution, Molecular , Kinetics , Models, Molecular , Molecular Sequence Data , Mutation , Oligonucleotides/chemistry , Plasmids/metabolism , Protein Binding , Protein Folding , Protein Structure, Tertiary , Sequence Homology, Amino Acid , Time Factors , UltracentrifugationABSTRACT
Saccharomyces cerevisiae RAD53 (CHK2) and CHK1 control two parallel branches of the RAD9-mediated pathway for DNA damage-induced G(2) arrest. Previous studies indicate that RAD9 is required for X-ray-associated sister chromatid exchange (SCE), suppresses homology-directed translocations, and is involved in pathways for double-strand break repair (DSB) repair that are different than those controlled by PDS1. We measured DNA damage-associated SCE in strains containing two tandem fragments of his3, his3-Delta5' and his3-Delta3'::HOcs, and rates of spontaneous translocations in diploids containing GAL1::his3-Delta5' and trp1::his3-Delta3'::HOcs. DNA damage-associated SCE was measured after log phase cells were exposed to methyl methanesulfonate (MMS), 4-nitroquinoline 1-oxide (4-NQO), UV, X rays and HO-induced DSBs. We observed that rad53 mutants were defective in MMS-, 4-NQO, X-ray-associated and HO-induced SCE but not in UV-associated SCE. Similar to rad9 pds1 double mutants, rad53 pds1 double mutants exhibited more X-ray sensitivity than the single mutants. rad53 sml1 diploid mutants exhibited a 10-fold higher rate of spontaneous translocations compared to the sml1 diploid mutants. chk1 mutants were not deficient in DNA damage-associated SCE after exposure to DNA damaging agents or after DSBs were generated at trp1::his3-Delta5'his3-Delta3'::HOcs. These data indicate that RAD53, not CHK1, is required for DSB-initiated SCE, and DNA damage-associated SCE after exposure to X-ray-mimetic and UV-mimetic chemicals.
Subject(s)
Cell Cycle Proteins/physiology , DNA Breaks, Double-Stranded , DNA Damage/radiation effects , Protein Kinases/physiology , Protein Serine-Threonine Kinases/physiology , Saccharomyces cerevisiae Proteins/physiology , Saccharomyces cerevisiae/physiology , Sister Chromatid Exchange/physiology , Ultraviolet Rays , 4-Nitroquinoline-1-oxide/toxicity , Cell Cycle Proteins/genetics , Checkpoint Kinase 1 , Checkpoint Kinase 2 , DNA Breaks, Double-Stranded/drug effects , DNA Breaks, Double-Stranded/radiation effects , DNA Damage/drug effects , Deoxyribonucleases, Type II Site-Specific/biosynthesis , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/physiology , Methyl Methanesulfonate/toxicity , Mutagens/toxicity , Protein Kinases/genetics , Protein Serine-Threonine Kinases/genetics , Recombination, Genetic/drug effects , Recombination, Genetic/radiation effects , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/radiation effects , Saccharomyces cerevisiae Proteins/genetics , Sister Chromatid Exchange/drug effects , Sister Chromatid Exchange/radiation effects , X-RaysABSTRACT
PCR-based mutagenesis is a cornerstone of molecular biology and protein engineering studies. Herein we describe a rapid and highly efficient mutagenesis method using type IIs restriction enzymes. A template gene is amplified into two separate PCR fragments using two pairs of anchor and mutagenic primers. Mutated sequences are located near the recognition site of a type IIs restriction enzyme. After digestion of two fragments with a type IIs enzyme, exposed cohesive ends that are complementary to each other are then ligated together to generate a mutated gene. We applied this method to introduce multiple site-directed mutations in EGFP and Bcl-2 family genes and observed perfect mutagenesis efficiency at the desired sites. This efficient and cost-effective mutagenesis method can be applied to a wide variety of structural and functional studies in cell physiology.
Subject(s)
Deoxyribonucleases, Type II Site-Specific/physiology , Mutagenesis, Site-Directed , Polymerase Chain Reaction/methods , Base Sequence , Cell Physiological Phenomena , DNA Primers , Multigene FamilyABSTRACT
The type II restriction-modification (R-M) system LlaDII isolated from Lactococcus lactis contains two tandemly arranged genes, llaDIIR and llaDIIM, encoding a restriction endonuclease (REase) and a methyltransferase (MTase), respectively. Interestingly, two LlaDII recognition sites are present in the llaDIIM promoter region, suggesting that they may influence the activity of the promoter through methylation status. In this study, separate promoters for llaDIIR and llaDIIM were identified, and the regulation of the two genes at the transcriptional level was investigated. DNA fragments containing the putative promoters were cloned in a promoter probe vector and tested for activity in the presence and absence of the active MTase. The level of expression of the MTase was 5- to 10-fold higher than the level of expression of the REase. The results also showed that the presence of M.LlaDII reduced the in vivo expression of the llaDIIM promoter (P(llaDIIM)) up to 1,000-fold, whereas the activity of the llaDIIR promoter (P(llaDIIR)) was not affected. Based on site-specific mutations it was shown that both of the LlaDII recognition sites within P(llaDIIM) are required to obtain complete repression of transcriptional activity. No regulation was found for llaDIIR, which appears to be constitutively expressed.
Subject(s)
DNA Modification Methylases/physiology , Deoxyribonucleases, Type II Site-Specific/genetics , Gene Expression Regulation , Base Sequence , Deoxyribonucleases, Type II Site-Specific/physiology , Homeostasis , Molecular Sequence Data , Mutagenesis, Site-Directed , Promoter Regions, Genetic , Transcription, GeneticABSTRACT
The widespread use of fish as model systems is still limited by the mosaic distribution of cells transiently expressing transgenes leading to a low frequency of transgenic fish. Here we present a strategy that overcomes this problem. Transgenes of interest were flanked by two I-SceI meganuclease recognition sites, and co-injected together with the I-SceI meganuclease enzyme into medaka embryos (Oryzias latipes) at the one-cell stage. First, the promoter dependent expression was strongly enhanced. Already in F0, 76% of the embryos exhibited uniform promoter dependent expression compared to 26% when injections were performed without meganuclease. Second, the transgenesis frequency was raised to 30.5%. Even more striking was the increase in the germline transmission rate. Whereas in standard protocols it does not exceed a few percent, the number of transgenic F1 offspring of an identified founder fish reached the optimum of 50% in most lines resulting from meganuclease co-injection. Southern blot analysis showed that the individual integration loci contain only one or few copies of the transgene in tandem. At a lower rate this method also leads to enhancer trapping effects, novel patterns that are likely due to the integration of the transgene in the vicinity of enhancer elements. Meganuclease co-injection thus provides a simple and highly efficient tool to improve transgenesis by microinjection.
Subject(s)
Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/physiology , Animals , Animals, Genetically Modified , Blotting, Southern , DNA/metabolism , Enhancer Elements, Genetic , Fishes , Green Fluorescent Proteins , Luminescent Proteins/metabolism , Microscopy, Fluorescence , Plasmids/metabolism , Promoter Regions, Genetic , Saccharomyces cerevisiae Proteins , Time Factors , Transgenes , ZebrafishABSTRACT
DNA looping is one of the key factors allowing proteins bound to different DNA sites to signal one another via direct contacts. We demonstrate that DNA looping can be generated in an arbitrary chosen site by sequence-directed targeting of double-stranded DNA with pseudocomplementary peptide-nucleic acids (pcPNAs). We designed pcPNAs to mask the DNA from cleavage by type IIs restriction enzyme PleI while not preventing the enzyme from binding to its primary DNA recognition site. Direct interaction between two protein molecules (one bound to the original recognition site and the other to a sequence-degenerated site) results in a totally new activity of PleI: it produces a nick near the degenerate site. The PNA-induced nicking efficiency varies with the distance between the two protein-binding sites in a phase with the DNA helical periodicity. Our findings imply a general approach for the fine-tuning of proteins bound to DNA sites well separated along the DNA chain.