Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 1.270
Filter
1.
BMJ Open ; 14(7): e083460, 2024 Jul 05.
Article in English | MEDLINE | ID: mdl-38969370

ABSTRACT

INTRODUCTION: Postoperative ileus (POI) is a postoperative complication that can cause lingering recovery after colorectal resection and a heavy healthcare system burden. Acupuncture aims to prevent postoperative complications, reduce the duration of POI, help recovery and shorten hospital stays. We hypothesise that preoperative electroacupuncture (EA) can promote POI recovery under the enhanced recovery after surgery protocol after laparoscopic surgery in patients with POI. METHODS AND ANALYSIS: This is a multicentre, randomised, sham-controlled trial. A total of 80 patients will be enrolled and randomly assigned to the EA or sham electroacupuncture (SA) group. The eligible patients will receive EA or SA for one session per day with treatment frequency starting on preoperative day 1 for four consecutive days. The primary outcome is the time to first defecation. The secondary outcomes include the time to first flatus, length of postoperative hospital stay, time to tolerability of semiliquid and solid food, postoperative nausea, vomiting, pain and extent of abdominal distention, time to first ambulation, preoperative anxiety, 30-day readmission rate, the usage of anaesthetics and analgesics during operation, length of postanaesthesia care unit stay. A mechanistic study by single-cell RNA sequencing in which postintervention normal intestinal tissue samples will be collected. The results of this study will provide evidence of the effects of acupuncture on POI and promote good clinical decision to millions of patients globally every year. ETHICS AND DISSEMINATION: This study has been approved by the ethical application of Beijing University of Chinese Medicine (2022BZYLL0401), Beijing Friendship Hospital Affiliated to Capital Medical University(2022-P2-368-02), Cancer Hospital Chinese Academy of Medical Science (23/175-3917), Huanxing Cancer Hospital (2023-002-02). The results will be published in a medical journal. In addition, we plan to present them at scientific conferences. TRIAL REGISTRATION NUMBER: ChiCTR2300077633.


Subject(s)
Colorectal Neoplasms , Electroacupuncture , Ileus , Laparoscopy , Postoperative Complications , Humans , Electroacupuncture/methods , Laparoscopy/adverse effects , Ileus/etiology , Ileus/therapy , Colorectal Neoplasms/surgery , Postoperative Complications/therapy , Postoperative Complications/etiology , China , Length of Stay/statistics & numerical data , Multicenter Studies as Topic , Randomized Controlled Trials as Topic , Preoperative Care/methods , Female , Adult , Male
2.
Medicine (Baltimore) ; 103(27): e38595, 2024 Jul 05.
Article in English | MEDLINE | ID: mdl-38968523

ABSTRACT

To observe of the effect of electrical stimulation at the back-shu acupoint with extrinsic diaphragmatic pacing (EDP) mode on respiratory function and extubation success rate in tracheostomized stroke patients. A total of 200 patients who underwent tracheostomy after a stroke from January 2022 to February 2023 were included in this study. They were divided into 2 groups based on whether electroacupuncture was used: the EDP + electroacupuncture group and the EDP group. We assessed the differences in cough reflex scores and clinical lung infection scores between the 2 groups, and measured levels of blood gas analysis indicators, diaphragmatic function, lung function, maximum inspiratory pressure, and maximum expiratory pressure in both groups. The total effective rate in the EDP + electroacupuncture group was 91.00% (91/100), which was higher than the EDP group's 80.00% (80/100) (P < .05). After treatment, both groups showed a decrease in clinical lung infection scores and cough reflex scores compared to before treatment, with the EDP + electroacupuncture group having lower scores than the EDP group (P < .05). After treatment, the pH value, arterial oxygen pressure, and oxygenation index all increased compared to before treatment, with the EDP + electroacupuncture group showing higher values than the EDP group (P < .05). After treatment, both groups showed a decrease in arterial carbon dioxide pressure compared to before treatment, with the EDP + electroacupuncture group having lower PaCO2 levels than the EDP group (P < .05). After treatment, both groups showed an increase in forced vital capacity as a percentage of predicted value (FVC%), diaphragm thickness, diaphragm mobility, maximum inspiratory pressure, maximum expiratory pressure, forced expiratory volume in the first second as a percentage of predicted value (FEV1%), and diaphragm contraction speed compared to before treatment. Additionally, the EDP + electroacupuncture group had higher values in these parameters compared to the EDP group (P < .05). The EDP + electroacupuncture group had a shorter average extubation time and a higher extubation success rate compared to the EDP group (P < .05). The combination of EDP mode and electroacupuncture at the back-shu acupoint appears to be effective in improving lung function and diaphragmatic function in tracheostomized stroke patients. It also leads to a shorter extubation time and higher extubation success rates.


Subject(s)
Acupuncture Points , Airway Extubation , Diaphragm , Electroacupuncture , Stroke , Tracheostomy , Humans , Male , Female , Middle Aged , Electroacupuncture/methods , Aged , Airway Extubation/methods , Diaphragm/physiopathology , Stroke/therapy , Tracheostomy/methods , Treatment Outcome , Respiratory Function Tests
3.
Medicine (Baltimore) ; 103(23): e38403, 2024 Jun 07.
Article in English | MEDLINE | ID: mdl-38847671

ABSTRACT

BACKGROUND: Electroacupuncture (EA) is considered as an effective treatment for urinary retention (UR). METHODS: Up to April 7, 2023, randomized controlled trials (RCTs) of EA for UR were extensively searched in 8 databases, including Pubmed, Cochrane, Embase, Web of Science, Chinese Biomedical Literature Database, Chinese Journal Full-text Database, Wanfang Data, and VIP Full-text e-Journals Database. The Cochrane Risk of Bias tool and the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) were utilized to evaluate the quality of all included RCTs. Meta-analysis was conducted using Stata 15.0 software. RESULTS: A total of 23 trials were included, and the meta-analysis results suggested that compared with the control group, EA can effectively treat various types of UR (risk ratio [RR] = 1.22, 95CI%: 1.14, 1.31) and promote bladder function recovery, with a significant reduce in residual urine volume (weighted mean differences [WMD] = -49.60, 95CI%: -64.10, -35.11), an increase in maximum bladder capacity (WMD = 47.00, 95CI%: 12.76, 81.24), a shorten in the first urination time (standardized mean difference [SMD] = -1.42, 95CI%: -2.08, -0.76), and less adverse reactions (RR = 0.21, 95CI%: 0.07, 0.65). CONCLUSION: EA has significant advantages in treating UR, but the efficacy and safety are still needed to be further verified through large-sample and high-quality RCTs.


Subject(s)
Electroacupuncture , Urinary Retention , Urinary Retention/therapy , Electroacupuncture/methods , Electroacupuncture/adverse effects , Humans , Randomized Controlled Trials as Topic , Treatment Outcome , Urination/physiology
4.
PLoS One ; 19(6): e0305105, 2024.
Article in English | MEDLINE | ID: mdl-38861546

ABSTRACT

PURPOSE: Knee osteoarthritis (KOA) is a common disorder among middle and older individuals. Electroacupuncture and exercise are present as two popular physical therapies for the management of KOA, and both were demonstrated to produce considerable results. However, the clinical decision-making process between these therapeutic interventions remains challenging due to the limited evidence of distinctions in their respective effects. This study aims to evaluate the clinical effect and cost effectiveness of electroacupuncture versus exercise in patients with KOA. STUDY DESIGN AND METHODS: This is a randomized controlled trial in which 196 symptomatic KOA patients will be randomly assigned 1:1 either to the electroacupuncture group (n = 98) and the exercise group (n = 98). Patients in the electroacupuncture group will receive acupuncture with electric stimulation 3 times a week for 8 weeks, whereas patients in the exercise group will receive neuromuscular training twice a week for 8 weeks. Education concerning KOA management will be provided in both therapies. Co-primary outcomes include changes in numerical rating scale (NRS) and Knee injury and Osteoarthritis Outcome Score (KOOS) Activities of Daily Living (ADL) subscale from baseline at week 8. Secondary outcomes include KOOS Pain subscale, KOOS knee-related Quality of Life (QOL) subscale, Short Form 6 Dimensions (SF-6D), five-level EuroQol five-dimensional questionnaire (EQ-5D-5L), Credibility/ Expectancy Questionnaire, Patient's global assessment (PGA), 30-second Chair Stand Test (30s-CST), 40m (4*10m) Fast Paced Walk Test (40m FPWT), and Daily Physical Activity level (DPA). DISCUSSION: The results of this study will provide evidence regarding differences between these 2 physical therapies in multiple aspects and will provide specific guidance for the development of treatments based on the needs of individual patients. TRIAL REGISTRATION: ChiCTR2300070376.


Subject(s)
Electroacupuncture , Exercise Therapy , Osteoarthritis, Knee , Quality of Life , Humans , Osteoarthritis, Knee/therapy , Electroacupuncture/methods , Exercise Therapy/methods , Female , Male , Middle Aged , Treatment Outcome , Aged , Activities of Daily Living , Adult , Exercise
5.
Med Sci Monit ; 30: e943748, 2024 Jun 10.
Article in English | MEDLINE | ID: mdl-38853414

ABSTRACT

BACKGROUND This study embarked on an innovative exploration to elucidate the effects of integrating electroacupuncture (EA) with motor training (MT) on enhancing corticospinal excitability and motor learning. Central to this investigation is the interplay between homeostatic and non-homeostatic metaplasticity processes, providing insights into how these combined interventions may influence neural plasticity and motor skill acquisition. MATERIAL AND METHODS The investigation enrolled 20 healthy volunteers, subjecting them to 4 distinct interventions to parse out the individual and combined effects of EA and MT. These interventions were EA alone, MT alone, EA-priming followed by MT, and MT-priming followed by EA. The assessment of changes in primary motor cortex (M1) excitability was conducted through motor-evoked potentials (MEPs), while the grooved pegboard test (GPT) was used to evaluate alterations in motor performance. RESULTS The findings revealed that EA and MT independently contributed to enhanced M1 excitability and motor performance. However, the additional priming with EA or MT did not yield further modulation in MEPs amplitudes. Notably, EA-priming was associated with improved GPT completion times, underscoring its potential in facilitating motor learning. CONCLUSIONS The study underscores that while EA and MT individually augment motor cortex excitability and performance, their synergistic application does not further enhance or inhibit cortical excitability. This points to the involvement of non-homeostatic metaplasticity mechanisms. Nonetheless, EA emerges as a critical tool in preventing M1 overstimulation, thereby continuously fostering motor learning. The findings call for further research into the strategic application of EA, whether in isolation or with MT, within clinical settings to optimize rehabilitation outcomes.


Subject(s)
Electroacupuncture , Evoked Potentials, Motor , Healthy Volunteers , Learning , Motor Cortex , Transcranial Magnetic Stimulation , Humans , Electroacupuncture/methods , Male , Motor Cortex/physiology , Learning/physiology , Female , Evoked Potentials, Motor/physiology , Adult , Transcranial Magnetic Stimulation/methods , Neuronal Plasticity/physiology , Young Adult , Motor Skills/physiology , Pyramidal Tracts/physiology
6.
Medicine (Baltimore) ; 103(24): e38263, 2024 Jun 14.
Article in English | MEDLINE | ID: mdl-38875369

ABSTRACT

To explore the clinical efficacy of scalp electroacupuncture combined with rehabilitation training for aphasia after head injury, and analyze its effect on patients' language function and quality of life. Our hospital randomly enrolled 100 aphasia patients caused by head injury treated from March 2020 to March 2022 as the experimental object and divided them into the control group and experimental group, with 50 cases in each group. The general rehabilitation training was performed to the control group and the scalp electroacupuncture combined with rehabilitation training was performed to the experimental group to compare their mini-mental state examination scores, communicative activities in daily living scores, aphasia battery of Chinese scores, quality of life scores, mental status scale in nonpsychiatric settings scores, National Institutes of Health Stroke Scale scores, effective rates, satisfaction of patients, and adverse reaction rates. In the between-group comparison, the patients in the experimental group had significantly higher mini-mental state examination scores, communicative activities in daily living scores, aphasia battery of Chinese scores, quality of life scores, effective rates, and satisfaction, and significantly lower mental status scale in nonpsychiatric settings scores, National Institutes of Health Stroke Scale scores and adverse reaction rates, which was statistically significant (P < .05 in all cases). The combination treatment of scalp electroacupuncture and rehabilitation training can effectively improve the language function and quality of life of patients with aphasia after head injury and remarkably enhance the treatment effect.


Subject(s)
Aphasia , Craniocerebral Trauma , Electroacupuncture , Quality of Life , Humans , Electroacupuncture/methods , Female , Male , Middle Aged , Aphasia/rehabilitation , Aphasia/etiology , Aphasia/therapy , Adult , Craniocerebral Trauma/complications , Craniocerebral Trauma/rehabilitation , Craniocerebral Trauma/psychology , Treatment Outcome , Scalp/injuries , Aged , Combined Modality Therapy , Language , Activities of Daily Living
7.
Medicine (Baltimore) ; 103(24): e38547, 2024 Jun 14.
Article in English | MEDLINE | ID: mdl-38875429

ABSTRACT

INTRODUCTION: Oculomotor nerve palsy (ONP) is often discovered in the ophthalmology department, manifested as ptosis with the same side, eyeball in the fixed external booth, or accompanied by limited inward, upward, and downward movements. The present case report described the effect of electroacupuncture (EA) on a breast cancer patient with ONP after chemotherapy. PATIENT CONCERNS: A 56-year-old breast cancer patient presented with severe ptosis and fixed right eye exotropia. Besides, it is challenging to perform the movement inward, upward, and downward, and with obvious diplopia. DIAGNOSES: The breast cancer patient was diagnosed with ONP, chemotherapy history. INTERVENTIONS: The patient was introduced to acupuncture department to receiving EA treatment. OUTCOMES: After 12 times of EA treatments, the symptom of ptosis was significantly improved, and the right upper eyelid can lift autonomously as same as the left eye. Besides, the patient's right lateral eye could move freely, and the symptoms of double vision disappeared. CONCLUSION: The case suggests that EA may be an effective alternative treatment for ONP.


Subject(s)
Blepharoptosis , Breast Neoplasms , Electroacupuncture , Oculomotor Nerve Diseases , Humans , Female , Middle Aged , Electroacupuncture/methods , Oculomotor Nerve Diseases/therapy , Oculomotor Nerve Diseases/etiology , Breast Neoplasms/drug therapy , Blepharoptosis/etiology , Blepharoptosis/chemically induced , Blepharoptosis/therapy , Antineoplastic Agents/adverse effects , Antineoplastic Agents/therapeutic use
8.
Neuroreport ; 35(11): 692-701, 2024 Aug 07.
Article in English | MEDLINE | ID: mdl-38874969

ABSTRACT

OBJECTIVE: Diabetic neuropathic pain (DNP) is one of the most prevalent symptoms of diabetes. The alteration of proteins in the spinal cord dorsal horn (SCDH) plays a significant role in the genesis and the development of DNP. Our previous study has shown electroacupuncture could effectively relieve DNP. However, the potential mechanism inducing DNP's genesis and development remains unclear and needs further research. METHODS: This study established DNP model rats by intraperitoneally injecting a single high-dose streptozotocin; 2 Hz electroacupuncture was used to stimulate Zusanli (ST36) and Kunlun (BL60) of DNP rats daily from day 15 to day 21 after streptozotocin injection. Behavioral assay, quantitative PCR, immunofluorescence staining, and western blotting were used to study the analgesic mechanism of electroacupuncture. RESULTS: The bradykinin B1 receptor (B1R) mRNA, nuclear factor-κB p65 (p65), substance P, and calcitonin gene-related peptide (CGRP) protein expression were significantly enhanced in SCDH of DNP rats. The paw withdrawal threshold was increased while body weight and fasting blood glucose did not change in DNP rats after the electroacupuncture treatment. The expression of B1R, p65, substance P, and CGRP in SCDH of DNP rats was also inhibited after the electroacupuncture treatment. CONCLUSION: This work suggests that the potential mechanisms inducing the allodynia of DNP rats were possibly related to the increased expression of B1R, p65, substance P, and CGRP in SCDH. Downregulating B1R, p65, substance P, and CGRP expression levels in SCDH may achieve the analgesic effect of 2 Hz electroacupuncture treatment.


Subject(s)
Diabetes Mellitus, Experimental , Down-Regulation , Electroacupuncture , Hyperalgesia , Rats, Sprague-Dawley , Receptor, Bradykinin B1 , Spinal Cord Dorsal Horn , Animals , Electroacupuncture/methods , Male , Spinal Cord Dorsal Horn/metabolism , Hyperalgesia/therapy , Hyperalgesia/metabolism , Diabetes Mellitus, Experimental/therapy , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Experimental/complications , Receptor, Bradykinin B1/metabolism , Receptor, Bradykinin B1/genetics , Diabetic Neuropathies/metabolism , Diabetic Neuropathies/therapy , Rats , Calcitonin Gene-Related Peptide/metabolism , Calcitonin Gene-Related Peptide/genetics , Substance P/metabolism
9.
Brain Res ; 1839: 149010, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38763503

ABSTRACT

OBJECTIVE: Cerebral blood perfusion (CBP) reduction is a prevalent complication following subarachnoid hemorrhage (SAH) in clinical practice, often associated with long-term cognitive impairment and prognosis. Electroacupuncture (EA), a widely utilized traditional Chinese therapy for central nervous system disorders, has demonstrated promising therapeutic effects. This study aims to investigate the therapeutic potential of EA in restoring CBP in SAH rats and to explore the mechanisms involving HIF-1α in this process. METHODS: Rats were randomly assigned to one of five groups, including Sham, SAH, EA, EA + Saline, and EA + dimethyloxallyl glycine (DMOG) groups. EA treatment was administered for 10 min daily, while DMOG were intraperitoneally injected. Behavioral tests, cerebral blood flow monitoring, vascular thickness measurement, western blotting, and immunofluorescence staining were conducted to assess the therapeutic effects of EA on cerebral blood flow. RESULTS: SAH resulted in elevated levels of HIF-1α, endothelin (ET), ICAM-1, P-SELECTIN, E-SELECTIN, and decreased level of eNOS in the brain. This led to cerebral vasospasm, decreased CBF, and cognitive deficits in the rat SAH model. EA intervention downregulated the expression of HIF-1α, ET, ICAM-1, P-SELECTIN, and E-SELECTIN, while increasing eNOS expression. This alleviated cerebral vasospasm, restored CBF, and improved cognitive function. However, the administration of the HIF-1α stabilizer (DMOG) counteracted the therapeutic effects of EA. CONCLUSION: EA promotes the recovery of cerebral blood flow after SAH injury, attenuates cerebral vasospasm, and accelerates the recovery of cognitive dysfunction, and its mechanism of action may be related to the inhibition of the HIF-1α signaling pathway.


Subject(s)
Cerebrovascular Circulation , Electroacupuncture , Hypoxia-Inducible Factor 1, alpha Subunit , Rats, Sprague-Dawley , Subarachnoid Hemorrhage , Animals , Subarachnoid Hemorrhage/complications , Subarachnoid Hemorrhage/metabolism , Electroacupuncture/methods , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Male , Rats , Cerebrovascular Circulation/physiology , Cerebrovascular Circulation/drug effects , Vasospasm, Intracranial/metabolism , Vasospasm, Intracranial/etiology , Vasospasm, Intracranial/therapy , Disease Models, Animal , Brain/metabolism
10.
Neuroreport ; 35(10): 664-672, 2024 07 01.
Article in English | MEDLINE | ID: mdl-38813905

ABSTRACT

Traditional Chinese medicine (TCM) has long been used to treat various diseases, including cerebral ischemia. The specific molecular mechanism of TCM in the treatment of cerebral ischemia, however, is still unclear. This study investigated the effects of gastrodin, electroacupuncture and their combination on cerebral ischemic rats. We used Nissl staining, immunohistochemical staining and immunoblotting to detect the expression changes of brain-derived neurotrophic factor (BDNF) and interleukin-6 (IL-6) in the frontal cortex. The results showed that the combination therapy of gastrodin and electroacupuncture significantly increased the number of Nissl-positive neurons and improved cell morphology compared with other groups. Mechanistically, we found that the combination of gastrodin and electroacupuncture treatment group can restore the abnormal morphology of neuronal cells caused by cerebral ischemia by rebalancing the expression levels of BDNF and IL-6. Our research indicates that gastrodin combined with electroacupuncture has a significant protective effect on cerebral ischemic injury in rats, possibly by regulating the expression of BDNF and IL-6. This combination therapy is superior to single-drug or electroacupuncture therapy.


Subject(s)
Benzyl Alcohols , Brain Ischemia , Brain-Derived Neurotrophic Factor , Disease Models, Animal , Electroacupuncture , Glucosides , Interleukin-6 , Rats, Sprague-Dawley , Animals , Electroacupuncture/methods , Benzyl Alcohols/pharmacology , Glucosides/pharmacology , Glucosides/therapeutic use , Brain-Derived Neurotrophic Factor/metabolism , Interleukin-6/metabolism , Male , Brain Ischemia/metabolism , Brain Ischemia/prevention & control , Rats , Combined Modality Therapy/methods , Stroke/metabolism , Neurons/drug effects , Neurons/metabolism
11.
Vet J ; 305: 106140, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38782236

ABSTRACT

General anesthesia and surgical stress can suppress the immunological response by acting both directly on the immune system and indirectly on the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system. Disturbance of the immune system during the perioperative period can lead to complications such as wound-healing disorders and infections up to sepsis. Effectiveness of acupuncture in regulating the immune function by increasing leukocyte numbers and inhibiting inflammatory response has been proven. This study aimed to explore the impact of electroacupuncture (EAP) on the dynamic balance of the immune system and immune cell populations in dogs undergoing surgery. Twelve healthy bitches scheduled for elective ovariectomy were divided into two groups according to whether (EAP, n=6) or not (CTR, n=6) a peri-operative electroacupuncture treatment was performed. Levels of leukocytes (neutrophils, monocytes, T- and B-cells) and immunoglobulins M (IgM) and A (IgA) were measured in blood samples collected before (T0), 1 h (T1) and 2.5 h (T2) after anesthesia induction. Leukocytes count decreased from T0 to T1 in both groups and restored within 1.5 h in EAP group whereas remained significantly lower in CTR group (P<0.02). In particular, neutrophils and monocytes increased in dogs receiving EAP (P<0.01) while T-cells decreased in CTR group (P<0.04) at T2. B-cells and cytotoxic T-cells decreased in EAP dogs (P<0.04) at T2. No differences in helper T-cells, IgM and IgA levels were recorded between groups and over time. Our results suggest a modulatory effect of EAP on the immune system which is early expressed on neutrophils, monocytes and T-cells.


Subject(s)
Electroacupuncture , Animals , Dogs , Electroacupuncture/veterinary , Electroacupuncture/methods , Female , Pilot Projects , Ovariectomy/veterinary , Leukocyte Count/veterinary , Immunoglobulin A/blood , Immunoglobulin M/blood
12.
Brain Res Bull ; 213: 110985, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38806118

ABSTRACT

INTRODUCTION: Paclitaxel (PTX) cannot effectively treat glioma because it cannot cross the bloodbrain barrier (BBB). A specific mode electroacupuncture stimulation (SMES) can temporarily open the BBB, thereby improving drug delivery to the brain. This study aimed to observe SMES-mediated accumulation of PTX in the brain and its anti-glioma effect and explore the role of the Hedgehog pathway. METHODS: The acupoint selectivity of SMES in opening the BBB was examined in normal rats. The penetration and anti-glioma activity were determined in a C6-Luc glioma rat model. SMES was performed using 2/100 Hz, 3 mA, 6-6 s, and 40 min The survival curve was analysed by the KaplanMeier method, brain tumour pathology and size was observed by HE staining, and in vivo imaging system respectively. RESULTS: SMES-induced BBB opening had acupoint selectivity. SMES could improve PTX accumulation in brain and SMES-mediated PTX delivery showed enhanced anti-glioma activity due to better brain penetration. Hedgehog pathway was involved in SMES-mediated PTX delivery by regulating Occludin expression. CONCLUSION: SMES at the head acupoints to deliver PTX is a feasible and effective method for treating glioma. The Hedgehog pathway may play a key role in SMES-mediated PTX delivery across the BBB.


Subject(s)
Antineoplastic Agents, Phytogenic , Blood-Brain Barrier , Brain Neoplasms , Electroacupuncture , Glioma , Hedgehog Proteins , Paclitaxel , Animals , Electroacupuncture/methods , Paclitaxel/pharmacology , Hedgehog Proteins/metabolism , Glioma/therapy , Glioma/metabolism , Brain Neoplasms/therapy , Brain Neoplasms/metabolism , Antineoplastic Agents, Phytogenic/pharmacology , Blood-Brain Barrier/drug effects , Blood-Brain Barrier/metabolism , Male , Rats , Signal Transduction/drug effects , Signal Transduction/physiology , Rats, Sprague-Dawley , Cell Line, Tumor , Brain/metabolism , Brain/drug effects
13.
Biol Res ; 57(1): 34, 2024 May 29.
Article in English | MEDLINE | ID: mdl-38812057

ABSTRACT

Studies have suggested that endoplasmic reticulum stress (ERS) is involved in neurological dysfunction and that electroacupuncture (EA) attenuates neuropathic pain (NP) via undefined pathways. However, the role of ERS in the anterior cingulate cortex (ACC) in NP and the effect of EA on ERS in the ACC have not yet been investigated. In this study, an NP model was established by chronic constriction injury (CCI) of the left sciatic nerve in rats, and mechanical and cold tests were used to evaluate behavioral hyperalgesia. The protein expression and distribution were evaluated using western blotting and immunofluorescence. The results showed that glucose-regulated protein 78 (BIP) and inositol-requiring enzyme 1α (IRE-1α) were co-localized in neurons in the ACC. After CCI, BIP, IRE-1α, and phosphorylation of IRE-1α were upregulated in the ACC. Intra-ACC administration of 4-PBA and Kira-6 attenuated pain hypersensitivity and downregulated phosphorylation of IRE-1α, while intraperitoneal injection of 4-PBA attenuated hyperalgesia and inhibited the activation of P38 and JNK in ACC. In contrast, ERS activation by intraperitoneal injection of tunicamycin induced behavioral hyperalgesia in naive rats. Furthermore, EA attenuated pain hypersensitivity and inhibited the CCI-induced overexpression of BIP and pIRE-1α. Taken together, these results demonstrate that EA attenuates NP by suppressing BIP- and IRE-1α-mediated ERS in the ACC. Our study presents novel evidence that ERS in the ACC is implicated in the development of NP and provides insights into the molecular mechanisms involved in the analgesic effect of EA.


Subject(s)
Disease Models, Animal , Electroacupuncture , Endoplasmic Reticulum Stress , Gyrus Cinguli , Neuralgia , Rats, Sprague-Dawley , Animals , Electroacupuncture/methods , Gyrus Cinguli/metabolism , Neuralgia/therapy , Male , Endoplasmic Reticulum Stress/physiology , Rats , Blotting, Western , Heat-Shock Proteins/metabolism , Protein Serine-Threonine Kinases/metabolism , Hyperalgesia/therapy , Endoplasmic Reticulum Chaperone BiP
14.
Mol Pain ; 20: 17448069241258113, 2024.
Article in English | MEDLINE | ID: mdl-38744426

ABSTRACT

Background: Recent studies have demonstrated that activated microglia were involved in the pathogenesis of central sensitization characterized by cutaneous allodynia in migraine. Activation of microglia is accompanied by increased expression of its receptors and release of inflammatory mediators. Acupuncture and its developed electroacupuncture (EA) have been recommended as an alternative therapy for migraine and are widely used for relieving migraine-associated pain. However, it remains rare studies that show whether EA exerts anti-migraine effects via inhibiting microglial activation related to a release of microglial receptors and the inflammatory pathway. Therefore, this study aimed to investigate EA' ability to ameliorate central sensitization via modulation of microglial activation, microglial receptor, and inflammatory response using a rat model of migraine induced by repeated epidural chemical stimulation. Methods: In the present study, a rat model of migraine was established by epidural repeated inflammatory soup (IS) stimulation and treated with EA at Fengchi (GB20) and Yanglingquan (GB34) and acupuncture at sham-acupoints. Pain hypersensitivity was further determined by measuring the mechanical withdrawal threshold using the von-Frey filament. The changes in c-Fos and ionized calcium binding adaptor molecule 1 (Ibal-1) labeled microglia in the trigeminal nucleus caudalis (TNC) were examined by immunflurescence to assess the central sensitization and whether accompanied with microglia activation. In addition, the expression of Ibal-1, microglial purinoceptor P2X4, and its associated inflammatory signaling pathway mediators, including interleukin (IL)-1ß, NOD-like receptor protein 3 (NLRP3), and Caspase-1 in the TNC were investigated by western blot and real-time polymerase chain reaction analysis. Results: Allodynia increased of c-Fos, and activated microglia were observed after repeated IS stimulation. EA alleviated the decrease in mechanical withdrawal thresholds, reduced the activation of c-Fos and microglia labeled with Ibal-1, downregulated the level of microglial purinoceptor P2X4, and limited the inflammatory response (NLRP3/Caspase-1/IL-1ß signaling pathway) in the TNC of migraine rat model. Conclusions: Our results indicate that the anti-hyperalgesia effects of EA ameliorate central sensitization in IS-induced migraine by regulating microglial activation related to P2X4R and NLRP3/IL-1ß inflammatory pathway.


Subject(s)
Disease Models, Animal , Electroacupuncture , Hyperalgesia , Inflammation , Microglia , Migraine Disorders , Rats, Sprague-Dawley , Receptors, Purinergic P2X4 , Animals , Electroacupuncture/methods , Receptors, Purinergic P2X4/metabolism , Microglia/metabolism , Hyperalgesia/therapy , Hyperalgesia/metabolism , Migraine Disorders/therapy , Migraine Disorders/metabolism , Male , Inflammation/metabolism , Inflammation/pathology , Inflammation/therapy , Central Nervous System Sensitization/physiology , Rats , NLR Family, Pyrin Domain-Containing 3 Protein/metabolism , Proto-Oncogene Proteins c-fos/metabolism
15.
Cell Mol Biol (Noisy-le-grand) ; 70(5): 111-118, 2024 May 27.
Article in English | MEDLINE | ID: mdl-38814228

ABSTRACT

Bioinformatics analysis was performed to reveal the underlying pathogenesis of type 2 diabetes (T2DM) dry eye(DE) and to predict the core targets and potential pathways for electroacupuncture (EA) treatment of T2DM DE, in which key targets such as Toll-likereceptor4 (TLR4), NF-κB and Tumor necrosis factor-α (TNF-α) may be involved. Next, streptozotocin and a high-fat diet were used to generate T2DM-DE rats. Randomly picked EA, fluorometholone, model, and sham EA groups were created from successfully modelled T2DM DE rats. Six more rats were chosen as the blank group from among the normal rats. The results of DE index showed that EA improved the ocular surface symptoms.HE staining showed that EA attenuated the pathological changes in the cornea, conjunctiva and lacrimal gland of T2DM DE rats. EA decreased the expression of TLR4, MyD88, P-NF-κB P65, and TNF-α in the cornea, conjunctiva, and lacrimal gland, in accordance with immunofluorescence and Western blot data. Thus, EA reduced ocular surface symptoms and improved pathological changes of cornea, conjunctiva, and lacrimal gland induced by T2DM DE inT2DM DE rats, and the mechanism may be related to the inhibition of overactivation of the TLR4/NF-κB signaling pathway by EA and thus attenuating ocular surface inflammation.


Subject(s)
Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Dry Eye Syndromes , Electroacupuncture , NF-kappa B , Signal Transduction , Toll-Like Receptor 4 , Tumor Necrosis Factor-alpha , Animals , Toll-Like Receptor 4/metabolism , Electroacupuncture/methods , NF-kappa B/metabolism , Dry Eye Syndromes/therapy , Dry Eye Syndromes/metabolism , Dry Eye Syndromes/pathology , Diabetes Mellitus, Type 2/metabolism , Diabetes Mellitus, Type 2/therapy , Diabetes Mellitus, Experimental/therapy , Diabetes Mellitus, Experimental/metabolism , Male , Tumor Necrosis Factor-alpha/metabolism , Inflammation/pathology , Inflammation/metabolism , Rats, Sprague-Dawley , Rats , Lacrimal Apparatus/metabolism , Lacrimal Apparatus/pathology , Conjunctiva/metabolism , Conjunctiva/pathology , Cornea/pathology , Cornea/metabolism , Myeloid Differentiation Factor 88/metabolism
16.
Arch Esp Urol ; 77(3): 256-262, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38715166

ABSTRACT

OBJECTIVE: This study aimed to investigate the effect of electroacupuncture combined with pelvic floor muscle exercise in the treatment of female overactive bladder (OAB). METHODS: The clinical data of 134 female patients with OAB admitted to our hospital from April 2022 to June 2023 were retrospectively analysed. The patients were divided into the combination group (n = 74) and the single group (n = 60). The general demographic data, total effective rate, pad weight, female sexual function index (FSFI) score, oxford muscle grading scale and incontinence impact questionnaire short form (IIQ-7) were collected. Propensity score matching (PSM) was used to match the baseline data of the two groups at 1:1 ratio, and t test, chi-square test and analysis of variance were used for calculation. RESULTS: A total of 90 patients were selected after PSM. No significant difference in baseline data was found between the two groups (p > 0.05). Before treatment, no significant difference in FSFI score, oxford muscle grading scale and IIQ-7 score was found between the two groups (p > 0.05). The total effective rate of the combination group was higher than that of the single group (p < 0.05). After 3 weeks and 1 month of treatment, in addition to orgasm and sexual desire, the scores of sexual excitement and sexual satisfaction in the combination group were higher than those in the single group (p < 0.05). The combination group displayed higher oxford muscle grading scale and lower IIQ-7 and pad weight than the single group, and the differences were statistically significant (p < 0.05). CONCLUSIONS: The effect of electroacupuncture stimulation combined with pelvic floor muscle exercise is more significant, which can alleviate urinary symptoms, reduce urine leakage, enhance pelvic floor muscle strength and alleviate sexual dysfunction.


Subject(s)
Electroacupuncture , Exercise Therapy , Muscle Strength , Pelvic Floor , Sexual Dysfunction, Physiological , Urinary Bladder, Overactive , Humans , Female , Retrospective Studies , Pelvic Floor/physiopathology , Urinary Bladder, Overactive/therapy , Electroacupuncture/methods , Middle Aged , Exercise Therapy/methods , Sexual Dysfunction, Physiological/etiology , Sexual Dysfunction, Physiological/therapy , Combined Modality Therapy , Aged , Adult
17.
Front Immunol ; 15: 1373876, 2024.
Article in English | MEDLINE | ID: mdl-38715602

ABSTRACT

Objective: The objective of this study was to investigate the impact of electro-acupuncture (EA) on sepsis-related intestinal injury and its relationship with macrophage polarization. Methods: A sepsis model was established using cecal ligation and puncture (CLP) to assess the effectiveness of EA. The extent of pathological injury was evaluated using Chiu's score, the expression of ZO-1 and Ocludin, and the impact on macrophage polarization was examined through flow cytometry and immunofluorescence staining. The expression of spermidine, one type of polyamine, and ornithine decarboxylase (ODC) was measured using ELISA and PCR. Once the efficacy was determined, a polyamine depletion model was created, and the role of polyamines was reassessed by evaluating efficacy and observing macrophage polarization. Results: EA treatment reduced the Chiu's score and increased the expression of ZO-1 and Ocludin in the intestinal tissue of septic mice. It inhibited the secretion of IL-1ß and TNF-α, promoted the polarization of M2-type macrophages, increased the secretion of IL-10, and upregulated the expression of Arg-1, spermidine, and ODC. However, after depleting polyamines, the beneficial effects of EA on alleviating intestinal tissue damage and modulating macrophage polarization disappeared. Conclusion: The mechanism underlying the alleviation of intestinal injury associated with CLP-induced sepsis by EA involves with the promotion of M2-type macrophage polarization mediated by spermidine expression.


Subject(s)
Disease Models, Animal , Electroacupuncture , Macrophages , Polyamines , Sepsis , Animals , Sepsis/therapy , Sepsis/metabolism , Sepsis/immunology , Mice , Macrophages/immunology , Macrophages/metabolism , Electroacupuncture/methods , Polyamines/metabolism , Male , Macrophage Activation , Intestines/pathology , Intestines/immunology , Mice, Inbred C57BL , Cytokines/metabolism
18.
Braz J Med Biol Res ; 57: e13238, 2024.
Article in English | MEDLINE | ID: mdl-38808885

ABSTRACT

Osteoarthritis (OA) is a highly prevalent joint disorder characterized by progressive degeneration of articular cartilage, subchondral bone remodeling, osteophyte formation, synovial inflammation, and meniscal damage. Although the etiology of OA is multifactorial, pro-inflammatory processes appear to play a key role in disease pathogenesis. Previous studies indicate that electroacupuncture (EA) exerts chondroprotective, anti-inflammatory, and analgesic effects in preclinical models of OA, but the mechanisms underlying these potential therapeutic benefits remain incompletely defined. This study aimed to investigate the effects of EA on OA development in a rat model, as well as to explore associated molecular mechanisms modulated by EA treatment. Forty rats were divided into OA, EA, antagomiR-214, and control groups. Following intra-articular injection of monosodium iodoacetate to induce OA, EA and antagomiR-214 groups received daily EA stimulation at acupoints around the knee joint for 21 days. Functional pain behaviors and chondrocyte apoptosis were assessed as outcome measures. The expression of microRNA-214 (miR-214) and its downstream targets involved in apoptosis and nociception, BAX and TRPV4, were examined. Results demonstrated that EA treatment upregulated miR-214 expression in OA knee cartilage. By suppressing pro-apoptotic BAX and pro-nociceptive TRPV4, this EA-induced miR-214 upregulation ameliorated articular pain and prevented chondrocyte apoptosis. These findings suggested that miR-214 plays a key role mediating EA's therapeutic effects in OA pathophysiology, and represents a promising OA treatment target for modulation by acupuncture.


Subject(s)
Apoptosis , Chondrocytes , Disease Models, Animal , Electroacupuncture , MicroRNAs , Osteoarthritis , Rats, Sprague-Dawley , TRPV Cation Channels , bcl-2-Associated X Protein , Animals , TRPV Cation Channels/metabolism , MicroRNAs/metabolism , Electroacupuncture/methods , Male , Osteoarthritis/therapy , Chondrocytes/metabolism , bcl-2-Associated X Protein/metabolism , Rats
19.
Biochim Biophys Acta Mol Basis Dis ; 1870(5): 167208, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38701956

ABSTRACT

OBJECTIVE: This study aims to investigate the cardiac protective effects and molecular mechanisms of electroacupuncture (EA) pre-treatment in lipopolysaccharide (LPS)-Induced Cardiomyopathy. METHODS AND RESULTS: Pre-treatment with EA was performed 30 min before intraperitoneal injection of LPS. Cardiac function changes in mice of the EA + LPS group were observed using electrocardiography, echocardiography, and enzyme linked immunosorbent assay (ELISA) and compared with the LPS group. The results demonstrated that EA pre-treatment significantly improved the survival rate of septic mice, alleviated the severity of endotoxemia, and exhibited notable cardiac protective effects. These effects were characterized by a reduction in ST-segment elevation on electrocardiography, an increase in ejection fraction (EF) and fraction shortening (FS) on echocardiography and a decrease in the expression of serum cardiac troponin I (cTn-I) levels. Serum exosomes obtained after EA pre-treatment were extracted and administered to septic mice, revealing significant cardiac protective effects of EA-derived exosomes. Furthermore, the antagonism of circulating exosomes in mice markedly suppressed the cardiac protective effects conferred by EA pre-treatment. Analysis of serum exosomes using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed a significant upregulation of miR-381 expression after EA pre-treatment. Inhibition or overexpression of miR-381 through serotype 9 adeno-associated virus (AAV9)-mediated gene delivery demonstrated that overexpression of miR-381 exerted a cardiac protective effect, while inhibition of miR-381 significantly attenuated the cardiac protective effects conferred by EA pre-treatment. CONCLUSIONS: Our research findings have revealed a novel endogenous cardiac protection mechanism, wherein circulating exosomes derived from EA pre-treatment mitigate LPS-induced cardiac dysfunction via miR-381.


Subject(s)
Cardiomyopathies , Electroacupuncture , Exosomes , Lipopolysaccharides , MicroRNAs , Animals , MicroRNAs/genetics , MicroRNAs/metabolism , Exosomes/metabolism , Exosomes/genetics , Electroacupuncture/methods , Mice , Cardiomyopathies/chemically induced , Cardiomyopathies/metabolism , Cardiomyopathies/therapy , Cardiomyopathies/pathology , Cardiomyopathies/genetics , Cardiomyopathies/prevention & control , Lipopolysaccharides/toxicity , Male , Mice, Inbred C57BL , Disease Models, Animal
20.
Brain Behav ; 14(5): e3504, 2024 May.
Article in English | MEDLINE | ID: mdl-38698583

ABSTRACT

BACKGROUND: Electroacupuncture (EA) has been shown to facilitate brain plasticity-related functional recovery following ischemic stroke. The functional magnetic resonance imaging technique can be used to determine the range and mode of brain activation. After stroke, EA has been shown to alter brain connectivity, whereas EA's effect on brain network topology properties remains unclear. An evaluation of EA's effects on global and nodal topological properties in rats with ischemia reperfusion was conducted in this study. METHODS AND RESULTS: There were three groups of adult male Sprague-Dawley rats: sham-operated group (sham group), middle cerebral artery occlusion/reperfusion (MCAO/R) group, and MCAO/R plus EA (MCAO/R + EA) group. The differences in global and nodal topological properties, including shortest path length, global efficiency, local efficiency, small-worldness index, betweenness centrality (BC), and degree centrality (DC) were estimated. Graphical network analyses revealed that, as compared with the sham group, the MCAO/R group demonstrated a decrease in BC value in the right ventral hippocampus and increased BC in the right substantia nigra, accompanied by increased DC in the left nucleus accumbens shell (AcbSh). The BC was increased in the right hippocampus ventral and decreased in the right substantia nigra after EA intervention, and MCAO/R + EA resulted in a decreased DC in left AcbSh compared to MCAO/R. CONCLUSION: The results of this study provide a potential basis for EA to promote cognitive and motor function recovery after ischemic stroke.


Subject(s)
Electroacupuncture , Infarction, Middle Cerebral Artery , Magnetic Resonance Imaging , Rats, Sprague-Dawley , Reperfusion Injury , Animals , Electroacupuncture/methods , Male , Rats , Reperfusion Injury/physiopathology , Reperfusion Injury/therapy , Reperfusion Injury/diagnostic imaging , Infarction, Middle Cerebral Artery/therapy , Infarction, Middle Cerebral Artery/physiopathology , Infarction, Middle Cerebral Artery/diagnostic imaging , Brain/physiopathology , Brain/diagnostic imaging , Brain Ischemia/therapy , Brain Ischemia/physiopathology , Brain Ischemia/diagnostic imaging , Disease Models, Animal , Nerve Net/physiopathology , Nerve Net/diagnostic imaging , Ischemic Stroke/therapy , Ischemic Stroke/physiopathology , Ischemic Stroke/diagnostic imaging , Hippocampus/diagnostic imaging , Hippocampus/physiopathology
SELECTION OF CITATIONS
SEARCH DETAIL
...