ABSTRACT
Peste des petits ruminants is an acute and highly contagious disease caused by the Peste des petits ruminants virus (PPRV). Host proteins play a crucial role in viral replication. However, the effect of fusion (F) protein-interacting partners on PPRV infection is poorly understood. In this study, we found that the expression of goat plasminogen activator urokinase (PLAU) gradually decreased in a time- and dose-dependent manner in PPRV-infected goat alveolar macrophages (GAMs). Goat PLAU was subsequently identified using co-immunoprecipitation and confocal microscopy as an F protein binding partner. The overexpression of goat PLAU inhibited PPRV growth and replication, whereas silencing goat PLAU promoted viral growth and replication. Additionally, we confirmed that goat PLAU interacted with a virus-induced signaling adapter (VISA) to antagonize F-mediated VISA degradation, increasing the production of type I interferon. We also found that goat PLAU reduced the inhibition of PPRV replication in VISA-knockdown GAMs. Our results show that the host protein PLAU inhibits the growth and replication of PPRV by VISA-triggering RIG-I-like receptors and provides insight into the host protein that antagonizes PPRV immunosuppression.IMPORTANCEThe role of host proteins that interact with Peste des petits ruminants virus (PPRV) fusion (F) protein in PPRV replication is poorly understood. This study confirmed that goat plasminogen activator urokinase (PLAU) interacts with the PPRV F protein. We further discovered that goat PLAU inhibited PPRV replication by enhancing virus-induced signaling adapter (VISA) expression and reducing the ability of the F protein to degrade VISA. These findings offer insights into host resistance to viral invasion and suggest new strategies and directions for developing PPR vaccines.
Subject(s)
Goat Diseases , Goats , Host-Pathogen Interactions , Peste-des-Petits-Ruminants , Peste-des-petits-ruminants virus , Urokinase-Type Plasminogen Activator , Viral Fusion Proteins , Animals , Adaptor Proteins, Signal Transducing/metabolism , DEAD Box Protein 58/metabolism , Goat Diseases/immunology , Goat Diseases/metabolism , Goat Diseases/virology , Goats/immunology , Goats/virology , Macrophages, Alveolar , Peste-des-Petits-Ruminants/immunology , Peste-des-Petits-Ruminants/metabolism , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/growth & development , Peste-des-petits-ruminants virus/immunology , Peste-des-petits-ruminants virus/metabolism , Protein Binding , Urokinase-Type Plasminogen Activator/genetics , Urokinase-Type Plasminogen Activator/metabolism , Viral Fusion Proteins/metabolismABSTRACT
The Peste des petits ruminant virus (PPRV) is a member of the Paramyxoviridae family and is classified into the genus Measles virus. PPRV predominantly infects small ruminants, leading to mortality rates of nearly 100%, which have caused significant economic losses in developing countries. Host proteins are important in virus replication, but the PPRV nucleocapsid (N) protein-host interacting partners for regulating PPRV replication remain unclear. The present study confirmed the interaction between PPRV-N and the host protein vimentin by co-immunoprecipitation and co-localization experiments. Overexpression of vimentin suppressed PPRV replication, whereas vimentin knockdown had the opposite effect. Mechanistically, N was subjected to degradation via the ubiquitin/proteasome pathway, where vimentin recruits the E3 ubiquitin ligase NEDD4L to fulfill N-ubiquitination, resulting in the degradation of the N protein. These findings suggest that the host protein vimentin and E3 ubiquitin ligase NEDD4L have an anti-PPRV effect.
Subject(s)
Nucleocapsid Proteins , Peste-des-petits-ruminants virus , Vimentin , Virus Replication , Nucleocapsid Proteins/metabolism , Nucleocapsid Proteins/genetics , Vimentin/metabolism , Vimentin/genetics , Animals , Peste-des-petits-ruminants virus/physiology , Peste-des-petits-ruminants virus/genetics , Peste-des-petits-ruminants virus/metabolism , Humans , Ubiquitination , Host-Pathogen Interactions , HEK293 Cells , Nedd4 Ubiquitin Protein Ligases/metabolism , Nedd4 Ubiquitin Protein Ligases/genetics , Cell Line , Peste-des-Petits-Ruminants/virology , Peste-des-Petits-Ruminants/metabolism , Protein BindingABSTRACT
The fusion of haemagglutinin-neuraminidase (HN) protein of peste des petits ruminant (PPR) virus with signaling lymphocyte activation molecules (SLAM) host cell receptor consequences the virus entry and multiplication inside the host cell. The use of synthetic SLAM homologous peptides (i.e., molecular decoy for HN protein of PPR virus) may check PPR infection at the preliminary stage. Hence, the predicted SLAM homologous peptides using bioinformatics tools were synthesized by solid phase chemistry with standard Merrifield's 9-fluorenylmethoxycarbonyl (Fmoc) chemistry and were purified by reverse phase high performance liquid chromatography. The secondary structures of synthesized peptides were elucidated by circular dichroism spectroscopy. The in vitro interactions of these peptides were studied through indirect Enzyme Linked Immuno Sorbent Assay (ELISA) and visual surface plasmon UV-visible spectroscopy. The SLAM homologous peptides were able to interact with the peste des petits ruminant virus (PPRV) with varying binding efficiency. The interaction of SLAM homologous peptide with the PPR virus was ascertained by the change in the plasmon color from red wine to purple during visual detection and also by bathochromic shift in absorbance spectra under UV-visible spectrophotometry. The cytotoxic and anti-PPRV effect of these peptides were also evaluated in B95a cell line using PPR virus (Sungri/96). The cytotoxic concentration 50 (CC50 ) value of each peptide was greater than 1000 µg mL-1 . The anti-PPRV efficiency of SLAM-22 was relatively high among SLAM homologous peptides, SLAM-22 at 25 µg mL-1 concentration showed a reduction of more than log10 3 virus titer by priming of B95a cell line while the use of SLAM-15 and Muco-17 at the same concentration dropped virus titer from log10 4.8 to log10 2.5 and log10 3.1 respectively. The concentration of SLAM homologous peptide (25 µg mL-1 ) to exert its anti-PPRV effect was much less than its CC50 level (>1000 µg mL-1 ). Therefore, the synthetic SLAM homologous peptides may prove to be better agents to target PPRV.
Subject(s)
Peste-des-Petits-Ruminants , Peste-des-petits-ruminants virus , Animals , Peste-des-petits-ruminants virus/metabolism , Peste-des-Petits-Ruminants/metabolism , Cell Line , Viral Proteins/metabolism , Peptides/pharmacology , Peptides/metabolism , GoatsABSTRACT
The enveloped morbilliviruses utilise conserved proteinaceous receptors to enter host cells: SLAMF1 or Nectin-4. Receptor binding is initiated by the viral attachment protein Haemagglutinin (H), with the viral Fusion protein (F) driving membrane fusion. Crystal structures of the prototypic morbillivirus measles virus H with either SLAMF1 or Nectin-4 are available and have served as the basis for improved understanding of this interaction. However, whether these interactions remain conserved throughout the morbillivirus genus requires further characterisation. Using a random mutagenesis approach, based on error-prone PCR, we targeted the putative receptor binding site for SLAMF1 interaction on peste des petits ruminants virus (PPRV) H, identifying mutations that inhibited virus-induced cell-cell fusion. These data, combined with structural modelling of the PPRV H and ovine SLAMF1 interaction, indicate this region is functionally conserved across all morbilliviruses. Error-prone PCR provides a powerful tool for functionally characterising functional domains within viral proteins.
Subject(s)
Cell Adhesion Molecules/metabolism , Hemagglutinins, Viral/metabolism , Peste-des-petits-ruminants virus/metabolism , Polymerase Chain Reaction/methods , Signaling Lymphocytic Activation Molecule Family Member 1/metabolism , Viral Fusion Proteins/metabolism , Animals , Host Microbial Interactions , Membrane Fusion , SheepABSTRACT
Peste des petits ruminants virus (PPRV) causes an acute and highly contagious disease in domestic and wild small ruminants throughout the world, mainly by invoking immunosuppression in its natural hosts. It has been suggested that the non-structural C protein of PPRV helps in evading host responses but the molecular mechanisms by which it antagonizes the host responses have not been fully characterized. Here, we report the antagonistic effect of PPRV C protein on the expression of interferon-ß (IFN-ß) through both MAVS and RIG-I mediated pathways in vitro. Dual luciferase reporter assay and direct expression of IFN-ß mRNA analysis indicated that PPRV C significantly down regulates IFN-ß via its potential interaction with MAVS and RIG-I signaling molecules. Results further indicated that PPRV C protein significantly suppresses endogenous and exogenous IFN-ß-induced anti-viral effects in PPRV, EMCV and SVS infections in vitro. Moreover, PPRV C protein not only down regulates IFN-ß but also the downstream cytokines of interferon stimulated genes 56 (ISG56), ISG15, C-X-C motif chemokine (CXCL10) and RIG-I mediated activation of IFN promoter elements of ISRE and NF-κB. Further, this study deciphers that PPRV C protein could significantly inhibit the phosphorylation of STAT1 and interferes with the signal transmission in JAK-STAT signaling pathway. Collectively, this study indicates that PPRV C protein is important for innate immune evasion and disease progression.
Subject(s)
Interferon-beta/metabolism , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/metabolism , Viral Nonstructural Proteins/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Animals , Chlorocebus aethiops , DEAD Box Protein 58/metabolism , HEK293 Cells , Humans , Receptors, Immunologic/metabolism , Signal Transduction , Vero CellsABSTRACT
Peste des petits ruminants (PPR) is an acute, highly infectious, and highly pathogenic disease, which mainly damages small ruminants such as goats and sheep. Hemagglutinin protein (H), the main antigenic protein of peste des petits ruminants virus (PPRV), has been a hot spot in the research of genetic engineering vaccine for PPRV. In this study, the silkworm baculovirus surface display technology is combined with the transmembrane structure of the silkworm baculovirus envelope protein GP64 and different characteristics of the promoters to display four kinds of fusion proteins, which contain Pph-H, Pph-HJ, Pie1-H, and Pie1-HJ. The fusion proteins displayed on baculovirus surface have been detected by western blotting, cell surface immunofluorescence, and immunogold electron microscopy. In addition, the dominant form of PPR H displayed on baculovirus surface has been determined which is fusion protein mediated by Pph containing the hemagglutinin protein and full-length GP64, Pph-H. Furthermore, by comparing the fluorescence intensity of binding of hemagglutinin protein and signaling lymphocyte activation molecules (SLAM) in Vero-SLAM cells by immunocytochemistry, Pph-H can be combined with the receptor protein of PPRV, SLAM. It provides technical support for displaying the different structure of hemagglutinin and exploring the key sites of hemagglutinin and SLAM binding. Meanwhile, it is important for exploring the pathogenesis and immune mechanism of PPRV.
Subject(s)
Baculoviridae/metabolism , Hemagglutinins/metabolism , Host Microbial Interactions , Peste-des-petits-ruminants virus/metabolism , Signaling Lymphocytic Activation Molecule Family/metabolism , Animals , Baculoviridae/genetics , Bombyx/virology , Chlorocebus aethiops , DNA, Recombinant/genetics , Protein Binding , Vero CellsABSTRACT
Peste-des-petits-ruminants virus (PPRV) haemagglutinin (H) protein mediates binding to cellular receptors and then initiates virus entry. To identify the key residues of PPRV H (Hv) protein of the Nigeria 75/1 strain involved in binding to receptors, interaction of the Hv and mutated Hv (mHv) proteins with receptors (SLAM and Nectin 4) and their mutants (mSLAM1, mSLAM2, mSLAM3 and mNectin 4) was investigated using surface plasmon resonance imaging (SPRi) and coimmunoprecipitation (co-IP) assays. The results showed that the Hv protein failed to interact with mSLAM3, but interacted at a strong or medium intensity with SLAM, mSLAM2, Nectin 4 and mNectin 4, and at a low level with mSLAM1. The mHv protein was unable to interact with SLAM and its mutants, but bound to Nectin 4 and mNectin 4 with medium and weak intensity, respectively. Further analysis showed that the Hv protein could precipitate mSLAM1, mSLAM2 and mNectin 4, but not mSLAM3. The mHv protein failed to coprecipitate with SLAM and its mutants. The binding activities of mNectin 4 and Nectin 4 to mHv were less than 30.36 and 51.94â% of the wild-type levels, respectively. Based on the results obtained, amino acids at positions R389, L464, I498, R503, R533, Y541, Y543, F552 and Y553 of H protein and I61, H62, L64, K76, K78, E123, H130, I210, A211, S226 and R227 in SLAM were identified to be essential for the speciï¬city of H-SLAM interaction, while the critical residues of H-Nectin 4 interaction require further study. These findings would improve our understanding of the invasive mechanisms of PPRV.
Subject(s)
Amino Acids/analysis , Cell Adhesion Molecules/metabolism , Hemagglutinins, Viral/metabolism , Peste-des-Petits-Ruminants/metabolism , Peste-des-petits-ruminants virus/metabolism , Receptors, Cell Surface/metabolism , Signaling Lymphocytic Activation Molecule Associated Protein/metabolism , Amino Acid Sequence , Amino Acids/chemistry , Amino Acids/metabolism , Animals , CHO Cells , Cell Adhesion Molecules/genetics , Cricetulus , HEK293 Cells , Humans , Mutant Proteins/metabolism , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/chemistry , Receptors, Cell Surface/genetics , Recombinant Proteins/metabolism , Signaling Lymphocytic Activation Molecule Associated Protein/genetics , Transfection , Virus InternalizationABSTRACT
Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants that is caused by peste des petits ruminants virus (PPRV). To date, the molecular mechanism of PPRV infection is still unclear. It is well known that host proteins might be involved in the pathogenesis process for many viruses. In this study, we first proved that nucleolin (NCL), a highly conserved host factor, interacts with the core domain of PPRV N protein through its C terminus and co-locates with the N protein in the nucleus of cells. To investigate the role of NCL in PPRV infection, the expression level of NCL was inhibited with small interfering RNAs of NCL, and the results showed that PPRV growth was improved. However, the proliferation of PPRV was inhibited when the expression level of NCL was improved. Further analysis indicated that the inhibitory effect of NCL on the PPRV was caused by stimulating the interferon (IFN) pathways in host cells. In summary, our results will help us to understand the mechanism of PPRV infection.
Subject(s)
Peste-des-Petits-Ruminants/metabolism , Peste-des-petits-ruminants virus/metabolism , Phosphoproteins/metabolism , RNA-Binding Proteins/metabolism , Ruminants/metabolism , Animals , Cell Line , Chlorocebus aethiops , HEK293 Cells , Humans , Interferons/metabolism , Nucleocapsid Proteins/metabolism , Ruminants/virology , Vero Cells , NucleolinABSTRACT
Peste des petits ruminants virus (PPRV) is a highly contagious disease that affects sheep and goats. To better understand PPRV replication and virulence, cyclophilin A (CypA), a multifunctional goat host protein, was selected for further studies. CypA has been reported to inhibit or facilitate viral replication. However, the precise roles of CypA during PPRV infection remain unclear. Our data show for the first time that CypA suppressed PPRV replication by its PPIase activity, and PPRV infection decreased CypA protein levels. Detailed analysis revealed that PPRV H protein was responsible for the reduction of CypA, which was dependent on the lysosome pathway. No interaction was identified between H and CypA. Furthermore, the 35-58 region of H was essential for the reduction of CypA. In conclusion, our findings identify the antiviral role of CypA against PPRV and provide key insights into how PPRV H protein antagonizes host antiviral response.
Subject(s)
Cyclophilin A/metabolism , Hemagglutinins/metabolism , Lysosomes/metabolism , Viral Proteins/metabolism , Virus Replication , Animals , Cells, Cultured , Gene Expression , Goat Diseases/virology , Goats , HEK293 Cells , Host Microbial Interactions , Humans , Kidney/cytology , Peste-des-petits-ruminants virus/chemistry , Peste-des-petits-ruminants virus/metabolismABSTRACT
Hemagglutinin protein (H), one of the two glycoproteins of peste des petits ruminants virus (PPRV), binds to its receptor on the host cell and acts as a major antigen that induces and confers highly protective immunity in the host. In order to delineate the epitopes on H protein, fine epitope mapping and conservation analysis of linear B-cell epitopes (BCEs) on PPRV H has been undertaken using biosynthetic peptides and rabbit anti-PPRV H sera. Thirteen linear BCEs were identified and their corresponding minimal motifs were located on the H protein of PPRV China/Tibet/Geg/07-30. Conservation analysis indicated that two of the 13 minimal motifs were conserved among 52 PPRV strains. Nine of the 13 peptides containing the minimal motifs were recognized using anti-PPRV serum from a goat immunized with PPRV vaccine strain Nigeria 75/1. Identified epitopes and their motifs improve our understanding of the antigenic characteristics of PPRV H and provide a basis for the development of epitope-based diagnostic assays and multiple epitopes vaccine.
Subject(s)
Epitopes, B-Lymphocyte/genetics , Hemagglutinins/metabolism , Peste-des-petits-ruminants virus/metabolism , Viral Proteins/metabolism , Amino Acid Sequence , Antigens, Viral , Conserved Sequence , Epitopes, B-Lymphocyte/chemistry , Epitopes, B-Lymphocyte/metabolism , Gene Expression Regulation, Viral , Hemagglutinins/chemistry , Hemagglutinins/genetics , Models, Molecular , Peste-des-petits-ruminants virus/genetics , Protein Conformation , Viral Proteins/geneticsABSTRACT
Peste des petits ruminants virus (PPRV) is a morbillivirus that produces clinical disease in goats and sheep. We have studied the induction of interferon-ß (IFN-ß) following infection of cultured cells with wild-type and vaccine strains of PPRV, and the effects of such infection with PPRV on the induction of IFN-ß through both MDA-5 and RIG-I mediated pathways. Using both reporter assays and direct measurement of IFN-ß mRNA, we have found that PPRV infection induces IFN-ß only weakly and transiently, and the virus can actively block the induction of IFN-ß. We have also generated mutant PPRV that lack expression of either of the viral accessory proteins (V&C) to characterize the role of these proteins in IFN-ß induction during virus infection. Both PPRV_ΔV and PPRV_ΔC were defective in growth in cell culture, although in different ways. While the PPRV V protein bound to MDA-5 and, to a lesser extent, RIG-I, and over-expression of the V protein inhibited both IFN-ß induction pathways, PPRV lacking V protein expression can still block IFN-ß induction. In contrast, PPRV C bound to neither MDA-5 nor RIG-I, but PPRV lacking C protein expression lost the ability to block both MDA-5 and RIG-I mediated activation of IFN-ß. These results shed new light on the inhibition of the induction of IFN-ß by PPRV.
Subject(s)
Fibroblasts/metabolism , Goat Diseases/virology , Interferon Type I/metabolism , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/metabolism , Animals , Fibroblasts/virology , Goat Diseases/metabolism , Goats , Peste-des-Petits-Ruminants/metabolismABSTRACT
Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells. The co-expression of all four proteins resulted in the release of virus-like particles (VLPs) with similar release efficiency to that of authentic virions. Moreover, the co-expression of M together with F also resulted in efficient VLPs release. In the absence of M protein, the expression of no combination of the other proteins resulted in particle release. In summary, a VLPs production system for PPRV has been established and M protein is necessary for promoting the assembly and release of VLPs, of which the predominant protein is M protein. Further study will be focused on the immunogenicity of the VLPs.
Subject(s)
Peste-des-petits-ruminants virus/metabolism , Peste-des-petits-ruminants virus/physiology , Vero Cells/metabolism , Viral Matrix Proteins/metabolism , Animals , Antibodies, Viral , Chlorocebus aethiops/metabolism , Chlorocebus aethiops/physiology , DNA, Complementary , DNA, Viral , Hemagglutinins, Viral/metabolism , Hemagglutinins, Viral/physiology , Mice , Nucleocapsid Proteins/metabolism , Nucleocapsid Proteins/physiology , Peste-des-petits-ruminants virus/genetics , Peste-des-petits-ruminants virus/immunology , Viral Fusion Proteins/metabolism , Viral Fusion Proteins/physiologyABSTRACT
Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenoviruses serotype 5 (Ad5) expressing either the highly immunogenic fusion protein (F) or hemagglutinin protein (H) from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.
Subject(s)
Adenoviridae/genetics , Peste-des-Petits-Ruminants/immunology , Peste-des-petits-ruminants virus/immunology , Peste-des-petits-ruminants virus/metabolism , Viral Proteins/immunology , Animals , Female , Peste-des-Petits-Ruminants/prevention & control , Peste-des-petits-ruminants virus/genetics , Sheep , Viral Vaccines/immunology , Viral Vaccines/therapeutic useABSTRACT
Peste des petits ruminants virus (PPRV) is an important pathogen that seriously influences the productivity of small ruminants worldwide. Although PPRV is known to induce apoptosis in infected cells, the interaction between PPRV and permissive cells requires further elucidation. Here, we provide the first evidence that PPRV infection triggered autophagy in Vero cells based on the appearance of abundant double- and single-membrane vesicles, the accumulation of LC3 fluorescent puncta, the enhancement of LC3-I/-II conversion, and autophagic flux. We further demonstrated that induction of autophagy with rapamycin significantly increased PPRV progeny yield and nucleocapsid (N) protein expression, while inhibition of autophagy with siRNA targeting ATG7 resulted in diametrically opposite results. Our data indicate that PPRV exploits the autophagy machinery to facilitate its own replication in host cells, thus the production efficiency of live attenuated PPRV vaccines may be improved by targeting the autophagic pathway.
Subject(s)
Autophagy , Peste-des-petits-ruminants virus/physiology , Virus Replication , Animals , Antigens, Viral/immunology , Antigens, Viral/metabolism , Autophagy/genetics , Chlorocebus aethiops , Microtubule-Associated Proteins/metabolism , Nucleocapsid Proteins/biosynthesis , Nucleocapsid Proteins/immunology , Peste-des-petits-ruminants virus/genetics , Peste-des-petits-ruminants virus/metabolism , RNA Interference , RNA, Small Interfering , Sirolimus/pharmacology , Ubiquitin-Activating Enzymes/genetics , Ubiquitin-Activating Enzymes/metabolism , Vero Cells , Virus Replication/drug effectsABSTRACT
The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The P gene is 1,655 nucleotides long with two overlapping open reading frames (ORFs). The first ORF is 1530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the "China/Tib/Gej/07-30" shares homology of 86.10%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and V proteins respectively. Several sequence motifs in the P genes were identified on the basis of conservation in the PPRVs and the morbilliviruses.
Subject(s)
Goat Diseases/virology , Peste-des-Petits-Ruminants/veterinary , Peste-des-petits-ruminants virus/genetics , Phosphoproteins/genetics , Viral Proteins/genetics , Amino Acid Sequence , Animals , China , Female , Goats , Molecular Sequence Data , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/chemistry , Peste-des-petits-ruminants virus/isolation & purification , Peste-des-petits-ruminants virus/metabolism , Phosphoproteins/chemistry , Phosphoproteins/metabolism , Sequence Analysis , Sequence Homology, Amino Acid , Viral Proteins/chemistry , Viral Proteins/metabolismABSTRACT
In this study, both partial and full-length nucleocapsid (N) gene of Peste des petits ruminants virus (PPRV) were cloned into pET33b vector and expressed in Escherichia coli (BL21) with the objective of replacing live PPRV antigen with recombinant protein in ELISA. The expressed proteins were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot by using a PPRV N protein specific monoclonal antibody. The expressed histidine-tagged fusion proteins were purified using affinity Ni-NTA column and were assessed for their conformation in terms of reactivity by ELISA. The immunogenicity of recombinant proteins was also assessed in rabbits and anti-N antibody response against PPRV was observed in all the immunized rabbits, when tested by competitive and indirect ELISAs. In sandwich ELISA, a mean OD(492 nm) of 1.4 and 0.90 was obtained for crude lysate having expressed the N protein and the PPRV antigen, respectively. Further, the N protein was tested as a coating antigen in competitive ELISA instead of PPRV antigen for serological diagnosis of PPR infection. This indicates the diagnostic potential of the PPRV recombinant N proteins, which are safe and better alternatives to live PPRV antigen in ELISA for clinical or sero-surveillance of PPR in enzootic or non-enzootic countries.
Subject(s)
Escherichia coli/metabolism , Nucleocapsid Proteins , Peste-des-Petits-Ruminants/diagnosis , Peste-des-petits-ruminants virus/metabolism , Recombinant Proteins , Animals , Antibodies, Viral/blood , Antigens, Viral/genetics , Antigens, Viral/immunology , Antigens, Viral/metabolism , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Escherichia coli/genetics , Nucleocapsid Proteins/genetics , Nucleocapsid Proteins/immunology , Nucleocapsid Proteins/metabolism , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/genetics , Peste-des-petits-ruminants virus/immunology , Rabbits , Recombinant Proteins/genetics , Recombinant Proteins/immunologyABSTRACT
The large (L) polymerase gene and the 5'-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome. Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools.
Subject(s)
Genes, Viral , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/genetics , Viral Proteins/genetics , Amino Acid Sequence , Animals , Chlorocebus aethiops , DNA-Directed RNA Polymerases/biosynthesis , DNA-Directed RNA Polymerases/genetics , Genome, Viral , Molecular Sequence Data , Peste-des-Petits-Ruminants/metabolism , Peste-des-petits-ruminants virus/metabolism , Phylogeny , RNA, Viral/analysis , RNA, Viral/genetics , Sequence Alignment , Sequence Analysis, RNA , Vero CellsABSTRACT
An efficient and eco-friendly method for the synthesis of 4,4'-(arylmethylene)bis(1H-pyrazol-5-ols) has been accomplished by tandem Knoevenagel-Michael reaction of two equivalents of 5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one with various aromatic aldehydes catalyzed by ceric ammonium nitrate (CAN) in water. All the synthesized compounds 3a-j were evaluated for in vitro antiviral activity against peste des petits ruminant virus (PPRV). Compound 3i emerged as the most interesting compound in this series exhibiting excellent antiviral activity against PPRV and found to be more potent than the standard drug ribavirin used.
Subject(s)
Antiviral Agents/chemical synthesis , Chemistry, Pharmaceutical/methods , Peste-des-Petits-Ruminants/drug therapy , Peste-des-petits-ruminants virus/metabolism , Pyrazoles/chemical synthesis , Animals , Antiviral Agents/pharmacology , Cerium/chemistry , Chemistry, Organic/methods , Chlorocebus aethiops , Drug Design , Models, Chemical , Pyrazoles/pharmacology , Ribavirin/pharmacology , Vero Cells , Water/chemistryABSTRACT
The paramyxovirus P protein is an essential component of the transcriptase and replicase complex along with L protein. In this article, we have examined the functional roles of different domains of P proteins of two closely related morbilliviruses, Rinderpest virus (RPV) and Peste des petits ruminants virus (PPRV). The PPRV P protein physically interacts with RPV L as well as RPV N protein when expressed in transfected cells, as shown by co-immunoprecipitation. The heterologous L-P complex is biologically active when tested in a RPV minigenome replication/transcription system, only when used with PPRV N protein but not with RPV N protein. Employing chimeric PPRV/RPV cDNAs having different coding regions of P protein in the minigenome replication/transcription system, we identified a region between 290 and 346 aa in RPV P protein necessary for transcription of the minigenome.
Subject(s)
Peste-des-petits-ruminants virus/chemistry , Phosphoproteins/chemistry , Recombinant Fusion Proteins/chemistry , Rinderpest virus/chemistry , Animals , Cell Line , Humans , Nucleocapsid Proteins/genetics , Nucleocapsid Proteins/metabolism , Peste-des-petits-ruminants virus/genetics , Peste-des-petits-ruminants virus/metabolism , Phosphoproteins/genetics , Phosphoproteins/metabolism , Protein Structure, Tertiary , RNA-Dependent RNA Polymerase/genetics , RNA-Dependent RNA Polymerase/metabolism , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Rinderpest virus/genetics , Rinderpest virus/metabolism , SpodopteraABSTRACT
Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.