ABSTRACT
A new device was developed to hold linear transducers for transvaginal follicle aspiration. Efficacy of follicle aspiration was compared using a linear 6 MHz and a convex 5 MHz transducer. Fifty-five cows were submitted to follicle aspiration at random days of the estrous cycle. Aspirations were conducted with linear (n=28) and convex (n=38) transducers with 18 G needles at a negative pressure corresponding to 13 ml H(2)O/min. A greater number of follicles were aspirated using convex than to linear probe (12.4 versus 7.8, respectively, P<0.05). Mean number of oocytes and recovery rates were similar for convex (5.4 and 48.6%) and linear (4.6 and 59.3%) transducers. Limited space between the linear transducer and needle guide restricted access to some portions of the ovary, reducing the number of follicles aspirated using a linear transducer. The newly developed adaptor allowed greater stability, holding the ovaries firmly against the linear transducer. This diminished mobility permitted a similar number of oocytes to be recovered with both transducers. In conclusion, this new adaptor provided a low cost alternative for routine follicle aspiration and oocyte recovery in cattle.
Subject(s)
Cattle/surgery , Oocytes/physiology , Ovarian Follicle/surgery , Tissue and Organ Harvesting/veterinary , Animals , Cattle/physiology , Female , Oocytes/diagnostic imaging , Ovarian Follicle/diagnostic imaging , Tissue and Organ Harvesting/methods , Tissue and Organ Harvesting/standards , Transducers/standards , Transducers/veterinary , UltrasonographyABSTRACT
La glándula de Harder es una glándula túbulo-alveolar localizada en la parte posterior de la órbita ocular de animales que poseen membrana nictitante. En estos mamiferos la glándula contiene una gran cantidad de lípidos. La glándula de Harder de roedores contiene un pigmento café rojizo, el cual ha sido identificado como porfirina. Las funciones de la glándula de Harder son; síntesis y liberación de ferhormonas, fotoprotección y termorregulación, osmoprotección y se le ha propuesto además un papel inmunoendocrino