ABSTRACT
OBJECTIVES: The aim of this study is to assess the biological behaviour of porcine decellularized heterografts (Desc group) compared with cryopreserved homografts (Crio group) implanted in juvenile sheep. METHODS: Decellularized porcine pulmonary heterografts were implanted in five animals and cryopreserved pulmonary homografts in another five. The animals were followed-up for a mean of 280 +/- 14 days. The valve diameter was measured by echocardiography, which was performed at the 30th postoperative day, and before the explantation. The valves were also assessed macroscopically. Histological evaluation was performed using H.E., Gomori and Weigert staining. Immunohistochemistry specified different cell types (Factor VIII, CD3, Vimentin and CD68). Calcium quantity was analyzed using atomic absortion spectometry. RESULTS: There was one death in the Desc group due to endocarditis. The valves of Crio group showed decrease in the cellularity whereas the valves of Desc group showed matrix repopulation with endothelial and interstitial cells. Loss of collagen density and disarrangement of the normal fiber architecture was observed in Crio group. Calcium content demonstrated higher levels on the cusps and conduits in Crio group comparatively with Desc group. (P=0.016). The mean valvular diameter at the explantation was significantly increased (P=0.025) in the Desc group. CONCLUSIONS: Decellularized heterografts had a different biological behaviour when compared to cryopreserved homografts and become repopulated by cells with fibroblasts and endothelial cells characteristics. The matrix was preserved and some regenerative potential was present.
Subject(s)
Cryopreservation , Pulmonary Valve/transplantation , Tissue Engineering/methods , Transplantation, Heterologous/physiology , Transplantation, Homologous/physiology , Tricuspid Valve/surgery , Animals , Calcium/analysis , Collagen/metabolism , Extracellular Matrix/physiology , Fibroblasts/metabolism , Models, Animal , Sheep , Statistics, Nonparametric , Tricuspid Valve/metabolism , Tricuspid Valve/pathologyABSTRACT
OBJETIVO: Este estudo avalia o comportamento biológico dos heteroenxertos porcinos descelularizados (Grupo Desc) comparados com os homoenxertos criopreservados (Grupo Crio) implantados em carneiros jovens. MÉTODOS: Foram implantados em cinco animais heteroenxertos pulmonares porcinos descelularizados e em outros cinco, homoenxertos pulmonares criopreservados. Os animais apresentaram seguimento médio de 280 ± 14 dias. O diâmetro valvar foi medido por ecocardiografia, a qual foi realizada no 30º pós-operatório e antes do explante. As valvas foram também avaliadas macroscopicamente. A avaliação histológica foi realizada utilizando-se coloração de H.E., Gomori e Weigert e imunohistoquímica (Fator VIII, CD3, Vimentina e CD68). A quantificação de cálcio foi realizada utilizando-se espectrometria de absorção atômica. RESULTADOS: Houve um óbito no Grupo Desc por endocardite. As valvas do Grupo Crio apresentaram decréscimo na celularidade, enquanto que as valvas do Grupo Desc demonstraram repovoamento da matriz com células endoteliais e intersticiais. No grupo Crio, observou-se perda na densidade e desarranjo da arquitetura das fibras colágenas. A espectrometria de absorção atômica demonstrou maior calcificação no conduto e nas cúspides dos enxertos criopreservados quando comparados aos descelularizados (P=0,016). O diâmetro médio valvar no explante foi significantemente maior no Grupo Desc (P=0,025). CONCLUSÃO: Heteroenxertos descelularizados apresentam um comportamento biológico diferente quando comparados aos homoenxertos criopreservados, tornando-se repovoados por células com características de fibroblastos e células endoteliais. A matriz permaneceu bem preservada, o que possibilitou um processo de regeneração celular.
OBJECTIVES: The aim of this study is to assess the biological behaviour of porcine decellularized heterografts (Desc group) compared with cryopreserved homografts (Crio group) implanted in juvenile sheep. METHODS: Decellularized porcine pulmonary heterografts were implanted in five animals and cryopreserved pulmonary homografts in another five. The animals were followed-up for a mean of 280 ± 14 days. The valve diameter was measured by echocardiography, which was performed at the 30th postoperative day, and before the explantation. The valves were also assessed macroscopically. Histological evaluation was performed using H.E., Gomori and Weigert staining. Immunohistochemistry specified different cell types (Factor VIII, CD3, Vimentin and CD68). Calcium quantity was analyzed using atomic absortion spectometry. RESULTS: There was one death in the Desc group due to endocarditis. The valves of Crio group showed decrease in the cellularity whereas the valves of Desc group showed matrix repopulation with endothelial and interstitial cells. Loss of collagen density and disarrangement of the normal fiber architecture was observed in Crio group. Calcium content demonstrated higher levels on the cusps and conduits in Crio group comparatively with Desc group. (P=0.016). The mean valvular diameter at the explantation was significantly increased (P=0.025) in the Desc group. CONCLUSIONS: Decellularized heterografts had a different biological behaviour when compared to cryopreserved homografts and become repopulated by cells with fibroblasts and endothelial cells characteristics. The matrix was preserved and some regenerative potential was present
Subject(s)
Animals , Cryopreservation , Pulmonary Valve/transplantation , Tissue Engineering/methods , Transplantation, Heterologous/physiology , Transplantation, Homologous/physiology , Tricuspid Valve/surgery , Calcium/analysis , Collagen/metabolism , Extracellular Matrix/physiology , Fibroblasts/metabolism , Models, Animal , Sheep , Statistics, Nonparametric , Tricuspid Valve/metabolism , Tricuspid Valve/pathologySubject(s)
Humans , Alveolar Bone Loss , Biocompatible Materials , Bone Transplantation , Periodontal Diseases , Bone Substitutes , Wound Healing/physiology , Osteogenesis , Bone Morphogenetic Proteins/therapeutic use , Bone Regeneration/physiology , Transplantation, Autologous/physiology , Transplantation, Heterologous/physiology , Transplantation, Homologous/physiologySubject(s)
Humans , Bone Transplantation , Alveolar Bone Loss , Biocompatible Materials , Periodontal Diseases , Bone Substitutes , Bone Substitutes/therapeutic use , Osteogenesis , Bone Regeneration/physiology , Wound Healing/physiology , Transplantation, Autologous/physiology , Transplantation, Heterologous/physiology , Transplantation, Homologous/physiology , Bone Morphogenetic Proteins/therapeutic useABSTRACT
Xenografting is increasingly being developed as a response to the shortage of human tissues. However, antigenic components of bone material eliciting immune responses--particularly of cellular nature--are blamed for the reduction of the osteoinductive properties of bone and bone-derived implants. The aim of our study was to compare the immunologic response and osteogenesis induced by antigen-depleted allogeneic and xenogeneic bone-derived implants to that induced by partially antigen-depleted material heterotopically placed (muscular pouch) in rats. Wistar rats received bone-derived implants of different antigeneic condition, from both xenogeneic (rabbit) and allogeneic (rat) origin. After sacrifice, animals were evaluated for osteogenesis and immune response. New bone formation was observed around all bone-derived implants, whether fully or partially antigen-extracted, and from both xenogeneic and allogeneic origin. No significant humoral response resulted following bone implantation. Cellular response showed a similar pattern in partial and fully antigen-extracted bone of both allogeneic and xenogeneic origin. Xenogeneic antigen-extracted bone from safe donor sources could be a suitable solution to human tissue shortage in a near future.
Subject(s)
Bone Transplantation/physiology , Transplantation, Heterologous/physiology , Transplantation, Homologous/physiology , Animals , Bone Transplantation/immunology , Bone Transplantation/pathology , Osteogenesis , Rabbits , Rats , Rats, Wistar , Transplantation, Heterologous/immunology , Transplantation, Heterologous/pathology , Transplantation, Homologous/immunology , Transplantation, Homologous/pathologySubject(s)
Humans , Animals , Mice , Biotechnology , Transplantation Chimera/immunology , Transplantation, Heterologous/adverse effects , Transplantation, Heterologous/physiology , Transplantation, Heterologous/immunology , Transplantation, Heterologous/methods , Transplantation, Heterologous/psychology , Transplantation, Heterologous , Graft Rejection , Haplorhini , Mutation/genetics , SwineSubject(s)
Humans , Animals , Mice , Transplantation, Heterologous/methods , Transplantation, Heterologous/statistics & numerical data , Transplantation, Heterologous/adverse effects , Transplantation, Heterologous/immunology , Transplantation, Heterologous/physiology , Transplantation, Heterologous/psychology , Biotechnology/methods , Transplantation Chimera/immunology , Graft Rejection/immunology , Mutation/genetics , Swine , HaplorhiniSubject(s)
Cell Transplantation/methods , Diabetes Mellitus, Type 1/surgery , Hepatic Encephalopathy/surgery , Islets of Langerhans Transplantation/methods , Liver/cytology , Transplantation, Heterologous/methods , Animals , Capsules , Cell Separation , Galactosamine/toxicity , Hepatectomy , Islets of Langerhans/cytology , Islets of Langerhans Transplantation/physiology , Liver Function Tests , Liver, Artificial , Pancreas, Artificial , Pancreatectomy , Rats , Rats, Sprague-Dawley , Swine , Transplantation, Heterologous/physiologySubject(s)
Cell Transplantation , Hepatic Encephalopathy/therapy , Liver/cytology , Transplantation, Heterologous/methods , Alginates , Animals , Biopolymers , Capsules , Galactosamine , Graft Survival , Hepatic Encephalopathy/chemically induced , Liver Function Tests , Polylysine/analogs & derivatives , Rats , Rats, Sprague-Dawley , Survival Rate , Swine , Time Factors , Transplantation, Heterologous/physiologyABSTRACT
Foram interpostos 20 segmentos de artéria caudal bovina (ACB), tratadas com glutaraldeído e formaldeído, nas artérias femorais de dez cÝes. O período de segmento pós-operatório variou de 180 a 297 dias. foram avaliados: FunçÝo motora, pulsos (à palpaçÝo e ao ultra-sonar), variaçSes das pressSes arteriais sistólica,diastólica e média e fluxo através do enxerto, assim como, a consitência, a dilataçÝo e a coloraçÝo. NÝo houve óbitos, alteraçSes da marcha, necrose das patas ou processos inflamatórios nas feridas. Houve trombose total em 15por cento,trombose mural focal em 30 por cento, reaçÝo linfoplasmocitária focal da adventícia em 100 por cento, calcificaçÝo unicamente das miocélulas em 5 por cento, reendotelizaçÝo total em 60 por cento e parcial em 30 por cento dos enxertos. Em funçÝo dos resultados, concluímos que a ABC por apresentar adequaçÝo com diâmetro de pequenas artérias, ter comprimento suficiente para substituir ao atingir diversos segmentos de artérias distais, apresentar flexibilidade e rápida endotelizaçÝo indica a possivilidade de sua aplicaçÝo clínica como heteroenxerto vascular alternativo