A pilot-scale assessment of five common weeds in the sustainable treatment of sewage utilizing SHEFROL®, with prospects of a closed-loop biorefinery.

Relative efficacy of five common weeds-of the kind that are either rooted in soil or which freely float over water-was assessed in rapid, effective and sustainable treatment of sewage at pilot plant scale in the recently developed and patented SHEFROL® bioreactors. The plants were utilized in a unit of capacity 12,000 liters/day (LPD) which, after two years of use, was enlarged to handle 40,000 LPD of sewage. It was then further expanded after an year to treat 57,000 LPD. All the five weeds, of which none has previously been tested in a pilot-scale SHEFROL, were able to foster highly efficient primary treatment (in terms of suspended and total solids) and secondary treatment (in terms of BOD and COD) to levels exceeding 85% in most cases. Additionally, the weeds also helped in achieving significant tertiary treatment. At different hydraulic retention times, and at steady state, the five weeds achieved treatment of BOD, COD, suspended solids, nitrogen, phosphorous, copper, nickel, zinc, and manganese in the ranges, 80-95, 79-91, 82-95, 61-71, 51-73, 37-43, 30-38, 39-47, and 27-35%, respectively. It all occurred in a single process step and without the use of any machine or chemical. This made the system not only simple and inexpensive to install but also to maintain. Over continuous long-term operation for four years, the system was seen to be very robust as it was able to handle wide variations in the volumes and characteristics of sewage, as well as absorb shock loads without compromising the reactor performance. The sustainability of the system can be further enhanced by upgrading it to a circular biorefinery. Energy sources in the form of volatile fatty acids (VFAs) can be extracted from the weeds removed from SHEFROL and then the weeds can be converted into organic fertilizer using high-rate vermireactors recently developed by the authors.

A novel and inexpensive, yet very efficient sewage treatment system is presented.The versatility and robustness of the system has been assessed at pilot plant scale for several years.The long-term continuous studies establish the efficacy of five common weeds­not hitherto explored at pilot plant level­which can serve as the main bioagent(s) in the sewage treatment system.The system has the potential of being transformed in to a closed-loop-no-waste biorefinery.

AI-based prediction of the improvement in air quality induced by emergency measures.

Several cities in the developing world, of which the capital city of India, New Delhi, is an example, often experience air quality in which pollutant levels go way above the levels considered hazardous for human health. To bring down the air quality to within permissible limits quickly, the measures typically taken involve shutting down certain high-polluting activities for some time to enable the air quality to recover temporarily. This paper presents a first-ever model based on artificial neural networks to forecast the extent of reduction in air quality parameters that can be achieved and the time period within which a change can be experienced when the source of the emissions is cut off temporarily. The model is based on the extensive data on the extent of reduction in air quality parameters that occurred during the lockdown that was imposed during the COVID-19 pandemic. The non-linear autoregressive exogenous network-based model chosen for the purpose employs the hour since stopping of emissions, relative humidity, wind speed, wind direction, and ambient temperature as input parameters to predict the rate of change of PM2.5 with respect to the concentration at the start of the stopping of the emissions. Air quality data from a key monitoring station in New Delhi was used to develop the model. The model predicted the rate of drop in PM2.5 with an R and MSE of 0.0044 and 0.9736, respectively, while training and 0.0095 and 0.9583 while testing. The model was then tested with data from 19 other stations in New Delhi, and accuracy of the model was found to be exceptionally accurate, with the correlation between the measured and the predicted PM2.5 levels ranging from 0.74 to 0.94 and the MSE ranging from 0.0110 to 1.0746. Thus, the model can be employed to determine the number of hours of temporary stoppage of emissions required for the PM2.5 concentration to reach safe levels. The methodology of development of the model can be extrapolated to construct models tailored for use in other parts of the world as well.

An inexpensive phytoremediation system for treating 50,000 L/day of sewage.

The paper describes the setting up and long-term continuous operation of the first real-life, pilot scale, sewage treatment plant based on the recently patented phytoremediation technology, trademarked as SHEFROL®. The unit was about three times cheaper to install, operate and maintain than the least expensive of the other wetland-based technologies presently in vogue. Its semi-permanent version is 30 times cheaper. Monitoring of flow rates and levels of treatment intermittently over a 3 year course of continuous operation indicated the constancy and robustness of the reactor in treating total solids, suspended solids, chemical oxygen demand, biological oxygen demand, total Kjeldahl nitrogen, and soluble phosphorous to the average extents of 94, 84, 79, 70, 62 and 28% respectively. Earlier experience with bench-scale SHEFROL® units has indicated that removal of metals like Cu, Ni, Co, Zn, and Mn also takes place to the extent of 25-45% in these systems. These primary, secondary, and tertiary treatments occurred in a single unit process with no necessity of any pumping, aeration, or recycling. Models based on artificial intelligence were developed which enable forecasting the reactor performance in terms of secondary and tertiary treatment, respectively.

The paper describes the setting-up, long-term (3 years) performance evaluation at pilot scale, and modeling of a recently patented novel and unprecedented phytoremediation-based sewage treatment technology. The system is seen to be much more robust, versatile, space efficient, and economical, than existing macrophyte-based sewage treatment systems.

An Analysis of Failures Leading to Fire Accidents in Hospitals; with Specific Reference to India.

Fire can be especially dangerous when it occurs in hospitals because many patients in any typical hospital are not physically fit enough to quickly respond to emergency measures, especially evacuation calls. The present paper reports an in-depth assessment of the factors which have led to major fire accidents in Indian hospitals. The study reveals that several building safety codes, acts and guidelines are available, not only to prevent accidental fires but also to minimize harm when such fires do take place. However, observance of the stipulations is very lax, and seems to be exercised more in breach than in compliance. The study reveals that hospitals have zones like the intensive care units which are not only more prone to accidents than other zones but can also cause greater loss of lives due to the presence of critically ill patients, or persons who are extremely vulnerable (for instance newborn babies). Special codes and practices need to be framed for such zones. The study has also identified and catalogued a series of measures which must be implemented in future to prevent accidental fires in hospitals. The study is with reference to accidents that have occurred in India from 2010 to the present but is representative of the situation prevailing in most developing countries.

Elemental analysis of sage (herb) using calibration-free laser-induced breakdown spectroscopy.

In this work, laser-induced breakdown spectroscopy (LIBS) has been used for the quantitative and qualitative analysis of the sage sample using the calibration-free LIBS (CF-LIBS) technique. The sage plasma is generated by focusing the second harmonics (532 nm) of a Q-switched Nd:YAG laser with a repetition rate of 10 Hz and pulse duration of 5 ns. The emission spectra are recorded using a LIBS 2000 detection system spectrometer consisting of five high-resolution spectrometers covering a wavelength range from 200 to 720 nm. The optical emission spectra of the sage sample reveal the spectral lines of Fe, Ca, Ti, Co, Mn, Ni, and Cr. The plasma temperature and electron number density of the neutral spectral lines of the pertinent elements have been deduced using the Boltzmann plot and Stark-broadening line profile method, with average values 8855±885K and 3.89×1016cm-3, respectively. The average values of the plasma parameters were used for the quantification of the detected elements in the sample. Based on the calibration-free method, the measured results demonstrate that Fe is the major constituent in the sample, having a percentage concentration of 48.1%, while the remaining elements are Ca, Ti, Co, Mn, Ni, and Cr, with percentage concentrations 0.7%, 5.3%, 8%, 11%, 12.3%, and 14.6%, respectively. This study demonstrates the feasibility of LIBS for the compositional analysis of major and trace elements present in the plant samples and its further applications in medicine.

Vermicompost of the widespread and toxic xerophyte prosopis (Prosopis juliflora) is a benign organic fertilizer.

Prior to the work described in this paper, no report has existed on the use of any xerophyte is generating vermicompost. Now these authors have been able to develop a process with which the highly invasive xerophyte prosopis (Prosopis juliflora) can be vermicomposted. But does prosopis vermicompost (PVC) have any fertilizer value, given that prosopis has strong allelopathy and toxicity? To seek an answer, the authors have assessed the effect of the PVC on the germination and early growth of five common food plants: brinjal (Solanum melongena), chilly (Capsicum annuum), cucumber (Cucumissativus), ladies finger (Abelmoschus esculentus), and tomato (Lycopersicon esculentum). Thereafter the tomato and the brinjal plants were grown to fruition to assess their yield and nutrient value. Whereas no germination of any seed occurred when the soil was fortified with prosopis leaves, there was 20-100% germination of seeds in different plant species when fertilized with equivalent quantities of PVC. The carbohydrate, potassium, and magnesium contents of prosopis fertilized brinjal and tomato fruits were significantly (p ≤ 0.5) higher than the levels of these nutrients present in the market produce. The prosopis vermicompost displayed pest-repellant attributes as well. The findings indicate that the millions of tonnes of prosopis leaves, which have no utility value and of which falling on earth is harmful to the soil, can be used to generate organic fertilizer.

Potential of joyweed Alternanthera sessilis for rapid treatment of domestic sewage in SHEFROL® bioreactor.

In a first-ever report on this subject, it is shown that a common amphibious plant joyweed (Alternanthera sessilis) can be used in rapid and efficient treatment of biodegradable wastewaters, typified by domestic sewage. The plant was effective when used indoors under artificial lighting, as well as outdoors. It enabled treatment of sewage, varying widely in strength (from 300 mg/L to 1800 mg/L in chemical oxygen demand), to the extent of 78.9-83.9%. It was also able to remove biological oxygen demand, suspended solids, phosphorous, nitrogen, and the heavy metal copper to the extent of 87%, 93%, 45%, and 43%, respectively. Over 99% of total coliforms, faecal coliforms, and faecal streptococci were also removed. The treatment was very swiftly achieved, at a hydraulic retention time of just 6 h, in the "sheet flow root level" (SHEFROL®) bioreactor developed earlier by us and of which a patent claim has been registered. The findings indicate that A. sessilis has the potential to affect primary, secondary, and tertiary treatment of domestic sewage along with significant pathogen removal in a single process step when used in SHEFROL® bioreactors.

Use of the terrestrial weed Alternanthera ficoidea in treating greywater in soil-less SHEFROL® bioreactors.

The ornamental plant Alternanthera ficoidea (also named A. tenella), which is common and widespread throughout the tropics and is being increasingly regarded as an invasive, problematic weed, has been explored as a bioagent in greywater treatment. In the recently developed SHEFROL® bioreactor, it was seen to treat greywater of varying strengths (250-1,300 mg/L chemical oxygen demand, COD) quickly and substantially to the extent of 75-77%. Biological oxygen demand (BOD), nitrogen, phosphorus, suspended solids, and heavy metals copper, nickel, manganese, and zinc were also removed to the extents of 83.5, 94.9, 33.1, 27.0, 44.8, 27.5, 38.2, and 43.2%, respectively. As all this was achieved in a single pot, single step, and in a simple reactor operation, at hydraulic retention times of a mere 6 h, it shows the process to be several times more efficient as well as potentially less expensive than the conventional treatment systems which utilize macrophytes in tanks or constructed wetlands.

Vermicomposting transforms allelopathic parthenium into a benign organic fertilizer.

Vermicompost, which had been derived solely by the action of the epigeic earthworm Eisenia fetida on parthenium (Parthenium hysterophorus), was tested for its impact on the germination and early growth of green gram (Vigna radiata), ladies finger (Abelmoschus esculentus) and cucumber (Cucumis sativus). Seedlings were germinated and grown in soil amended with 0 (control), 0.75, 1.5, 2, 4, 8, 20 and 40% (by weight) parthenium vermicompost. Even though parthenium is known to possess strong negative allelopathy, as also plant/animal toxicity in other forms, its vermicompost (VC) manifested none of these attributes. Rather the VC enhanced germination success, introduced plant-friendly physical features in the container media, increased biomass carbon, and was seen to promote early growth as reflected in several morphological and biochemical characteristics in plants which had received parthenium VC in comparison to those which had not. All these effects were statistically significant. Fourier Transform Infrared (FTIR) Spectrometry revealed that the phenols and the sesquiterpene lactones that are responsible for the negative allelopathic impact of parthenium were largely destroyed in the course of vermicomposting. FTIR spectra also indicated that lignin content of parthenium was reduced during its vermicomposting. The findings open up the possibility that several other invasives known for their negative allelopathy and toxicity may also produce vermicompost which may be plant-friendly and soil-friendly. It also makes it appear possible that the huge quantities of phytomass that is generated annually by parthenium can be gainfully utilized in producing organic fertilizer via vermicomposting, thereby providing a means of exercising some control over parthenium's rampant growth and invasion.

Transformation of toxic and allelopathic lantana into a benign organic fertilizer through vermicomposting.

In a first study of its kind, the composition of vermicompost derived solely from the toxic and allelopathic weed lantana has been investigated using UV-visible and Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) and differential scanning calorimetry (DSC), gas chromatography-mass spectometry (GC-MS), and scanning electron microscopy (SEM). The studies reveal that a sharp reduction in humification index, substantial mineralization of organic matter and degradation of complex aromatics such as lignin and polyphenols into simpler carbohydrates and lipids occur in the course of vermicomposting. GC-MS analysis shows significant fragmentation, bio-oxidation and molecular rearrangements of chemical compounds in vermicompost in comparison to those in lantana. SEM micrographs of vermicompost reflect strong disaggregation of material compared to the much better formed lantana matrices. The phenols and sesquiterpene lactones which are specifically responsible for the toxicity and allelopathy of lantana are seen to get significantly degraded in the course of vermicomposting - turning it into a plant-friendly organic fertilizer. The study leads to the possibility that the millions of tons of phytomass that is generated annually by lantana can be gainfully utilized in producing organic fertilizer via vermicomposting.

The role of NADPH-derived reactive oxygen species production in the pathogenesis of endometriosis: a novel mechanistic approach.

Endometriosis is defined as endometriotic tissue growing outside the uterine cavity. It is a common gynecological disorder in women of reproductive age and is associated with chronic pelvic pain and infertility. Despite several studies and theories to explain its cause, the exact pathogenesis of endometriosis remains unclear. Retrograde menstruation is the most plausible theory, however, it is not exclusive. The disparity between the actual prevalence of retrograde menstruation and the prevalence of endometriosis suggests that other factors may determine the susceptibility to endometriosis development. Oxidative stress has been associated with endometriosis. This study aimed to explore the role of NADPH oxidase family in the production of reactive oxygen species (ROS) and to determine whether ROS induce the proliferation of endometriotic implants via mammalian target of rapamycin (mTOR) signaling. Anonymous endometriotic tissue samples were collected from women undergoing laparoscopy for endometriosis. The samples were stained with dihydroethidium and fluorescent images of the slides were taken to detect ROS production. After extraction of RNA from the samples and c-DNA generation, quantitative real-time PCR, protein extraction and Western blot were performed to study gene and protein expression of NADPH oxidase 1 (NOX 1), mTOR and fibronectin. The results showed an increase in ROS levels and NOX 1 gene and protein expression in the endometriotic tissues compared to the normal surrounding tissue control. Also, mTOR and fibronectin, gene expression was found to be increased. Up regulation of NOX at gene and protein level leads to increased production of ROS in the endometriotic tissue, which in turn causes proliferation of the ectopic tissue via alteration of the mTOR signaling pathway. Increased fibronectin gene expression points towards tissue injury in endometriosis as compared to the normal surrounding tissue. This manuscript adds a new insight into the pathogenesis of endometriosis and serves as a background for development of new treatments for the disease-associated pain and infertility.

Diet and adipose tissue distributions: The Multi-Ethnic Study of Atherosclerosis.

BACKGROUND AND AIMS: Dietary quality affects cardiometabolic risk, yet its pathways of influence on regional adipose tissue depots involved in metabolic and diabetes risk are not well established. We aimed to investigate the relationship between dietary quality and regional adiposity. METHODS AND RESULTS: We investigated 5079 individuals in the Multi-Ethnic Study of Atherosclerosis (MESA) who had food-frequency questionnaires and measurement of pericardial fat and hepatic attenuation at the baseline study visit in MESA, as well as a subgroup with imaging for visceral and subcutaneous fat (N = 1390). A dietary quality score (DietQuality) was constructed to include established food group constituents of a Mediterranean-type diet. Linear models estimated associations of dietary score as well as its constituents with regional adiposity. Baseline mean age was 61 (± 10) years, and approximately half of the participants (47%) were male. Those with a higher DietQuality score were generally older, female, with a lower body mass index, C-reactive protein, and markers of insulin resistance. After adjustment, a higher DietQuality score was associated with lower visceral fat (lowest vs. highest dietary score quartile: 523.6 vs. 460.5 cm(2)/m; P < 0.01 for trend), pericardial fat (47.5 vs. 41.3 cm(3)/m; P < 0.01 for trend), lesser hepatic steatosis (by hepatic attenuation; 58.6 vs. 60.7 Hounsfield units; P < 0.01 for trend), but not subcutaneous fat (P = 0.39). Greater fruits, vegetables, whole grains, seeds/nuts and yogurt intake were associated with decreased adiposity, while red/processed meats were associated with greater regional adiposity. CONCLUSION: A higher quality diet pattern is associated with less regional adiposity, suggesting a potential mechanism of beneficial dietary effects on diabetes, metabolic, and cardiovascular risk.

Abdominal fat radiodensity, quantity and cardiometabolic risk: The Multi-Ethnic Study of Atherosclerosis.

BACKGROUND AND AIMS: Fat radiodensity, as measured by fat attenuation on computed tomography (CT), has emerged as a potential biomarker of "fat quality." We sought to characterize the relationship between fat radiodensity and quantity in subcutaneous, visceral, and intermuscular fat depots, and its role in inflammation, insulin resistance, and metabolic syndrome (MetS). METHODS AND RESULTS: We studied 1511 individuals from the Multi-Ethnic Study of Atherosclerosis who underwent CT for measurement of regional fat distribution and radiodensity, along with biomarker assessments and adjudication of incident metabolic syndrome (MetS). Linear, logistic and Cox regression analyses were used to measure association between fat radiodensity and (1) fat quantity, (2) biomarkers of cardiometabolic dysfunction, and (3) both prevalent and incident MetS. In each fat depot, radiodensity was strongly and inversely associated with quantity (e.g., visceral fat radiodensity vs. quantity: ρ = -0.82, P < 0.01). After adjustment for age, sex and race, lower visceral fat radiodensity was associated with greater C-reactive protein, leptin and insulin, but lower adiponectin (P < 0.01 for all). After full adjustment for cardiovascular disease risk factors, visceral (but not subcutaneous or intermuscular) fat radiodensity was associated with prevalent MetS (OR = 0.96, 95% CI = 0.93-0.99, P = 0.01). Moreover, lower visceral fat radiodensity was associated with incident MetS after the same adjustment (HR = 0.95, 95% CI 0.93-0.98, P < 0.01). However, this association became non-significant after further adjustment for visceral fat quantity. CONCLUSION: Fat radiodensity is strongly correlated with fat quantity and relevant inflammatory biomarkers. Fat radiodensity (especially for visceral fat) may be a complementary, easily assessed marker of cardiometabolic risk.

Visceral adiposity and left ventricular remodeling: The Multi-Ethnic Study of Atherosclerosis.

BACKGROUND AND AIMS: Visceral fat (VF) is a source of pro-inflammatory adipokines implicated in cardiac remodeling. We sought to determine the impact of visceral fat and subcutaneous fat (SQ) depots on left ventricular (LV) structure, function, and geometry in the Multi-Ethnic Study of Atherosclerosis (MESA). METHODS AND RESULTS: We performed a post-hoc analysis on 1151 participants from MESA with cardiac magnetic resonance quantification of LV mass and LV mass-to-volume ratio (LVMV, an index of concentricity) and computed tomographic-derived SQ and VF area. Multivariable regression models to estimate association between height-indexed SQ and VF area (per cm(2)/m) with height-indexed LV mass (per height(2.7)) and LVMV were constructed, adjusted for clinical, biochemical, and demographic covariates. We found that both VF and SQ area were associated with height-indexed LV mass (ρ = 0.36 and 0.12, P < 0.0001, respectively), while only VF area was associated with LVMV (ρ = 0.28, P < 0.0001). Individuals with above-median VF had lower LV ejection fraction, greater indexed LV volumes and mass, and higher LVMV (all P < 0.001). In multivariable models adjusted for weight, VF (but not SQ) area was associated with LV concentricity and LV mass index, across both sexes. CONCLUSION: Visceral adiposity is independently associated with LV concentricity, a precursor to heart failure. Further study into the role of VF in LV remodeling as a potential therapeutic target is warranted.

Vermicomposting eliminates the toxicity of Lantana (Lantana camara) and turns it into a plant friendly organic fertilizer.

In evidently the first study of its kind, vermicompost derived solely from a weed known to possess plant and animal toxicity was used to assess its impact on the germination and early growth of several plant species. No pre-composting or supplementation of animal manure was done to generate the vermicompost in order to ensure that the impact is clearly attributable to the weed. Whereas the weed used in this study, Lantana (Lantana camara), is known to possess strong negative allelopathy, besides plant/animal toxicity in other forms, its vermicompost was seen to be a good organic fertilizer as it increased germination success and encouraged growth of all the three botanical species explored by the authors - green gram (Vigna radiata), ladies finger (Abelmoschus esculentus) and cucumber (Cucumis sativus). In terms of several physical, chemical and biochemical attributes that were studied, the vermicompost appeared plant-friendly, giving best results in general when employed at concentrations of 1.5% in soil (w/w). Fourier transform infrared spectrometry revealed that the phenols and the sesquiterpene lactones that are responsible for the allelopathic impact of Lantana were largely destroyed in the course of vermicomposting. There is also an indication that lignin content of Lantana was reduced during its vermicomposting. The findings open up the possibility that the billions of tons of phytomass that is generated annually by Lantana and other invasives can be gainfully utilized in generating organic fertilizer via vermicomposting.

Control of amphibious weed ipomoea (Ipomoea carnea) by utilizing it for the extraction of volatile fatty acids as energy precursors.

Volatile fatty acids (VFAs), comprising mainly of acetic acid and lesser quantities of propionic and butyric acids, are generated when zoomass or phytomass is acted upon by acidogenic and acetogenic microorganisms. VFAs can be utilized by methanogens under anaerobic conditions to generate flammable methane-carbon dioxide mixtures known as 'biogas'. Acting on the premise that this manner of VFA utilization for generating relatively clean energy can be easily accomplished in a controlled fashion in conventional biogas plants as well as higher-rate anaerobic digesters, we have carried out studies aimed to generate VFAs from the pernicious weed ipomoea (Ipomoea carnea). The VFA extraction was accomplished by a simple yet effective technology, appropriate for use even by laypersons. For this acid-phase reactors were set, to which measured quantities of ipomoea leaves were charged along with water inoculated with cow dung. The reactors were stirred intermittently. It was found that VFA production started within hours of the mixing of the reactants and peaked by the 10(th) or 11(th) day in all the reactors, effecting a conversion of over 10% of the biomass into VFAs. The reactor performance had good reproducibility and the process appeared easily controllable, frugal and robust.

Direct, rapid and sustainable vermicomposting of the leaf litter of neem (Azadirachta indica).

The recently developed concept of high rate vermicomposting was successfully used to enable direct vermicomoposting of neem leaves-without any pre-composting or cow dung supplementation as previously reported processes had necessitated. All the three epigeic species of earthworms that were explored, Eudrilus eugeniae, Eisenia fetida and Perionyx excavatus, provided efficient vermicast production with no mortality, persistent gain in body mass and good fecundity over the 16 months long period of reactor operation. In this period, all reactors were pulse-fed at the solid retention time of 20 days and were operated in the pseudo discretized continuous operation protocol developed earlier by the authors. With this, it was possible to almost completely dampen the influence of natural biodegradation of the feed or grazing by the earthworm born in the vermireactors. The findings, thus, conclusively prove that, all-through, the brisk vermicomposting was caused almost entirely by the action of the 'parent' earthworms on fresh feed.

An eco-friendly method of synthesizing gold nanoparticles using an otherwise worthless weed pistia (Pistia stratiotes L.).

A biomimetic method of gold nanoparticles synthesis utilizing the highly invasive aquatic weed pistia (Pistia stratiotes) is presented. In an attempt to utilize the entire plant, the efficacy of the extracts of all its parts - aerial and submerged - was explored with different proportions of gold (III) solution in generating gold nanoparticles (GNPs). The progress of the synthesis, which occurred at ambient temperature and pressure and commenced soon after mixing the pistia extracts and gold (III) solutions, was tracked using UV-visible spectrophotometry. The electron micrographs of the synthesized GNPs revealed that, depending on the metal-extract concentrations used in the synthesis, GNPs of either monodispersed spherical shape were formed or there was anisotropy resulting in a mixture of triangular, hexagonal, pentagonal, and truncated triangular shaped GNPs. This phenomenon was witnessed with the extracts of aerial parts as well as submerged parts of pistia. The presence of gold atoms in the nanoparticles was confirmed from the EDAX and X-ray diffraction studies. The FT-IR spectral study indicated that the primary and secondary amines associated with the polypeptide biomolecules could have been responsible for the reduction of the gold (III) ions to GNPs and their subsequent stabilization.

Effect of vermicast generated from an allelopathic weed lantana (Lantana camara) on seed germination, plant growth, and yield of cluster bean (Cyamopsis tetragonoloba).

In perhaps the first-ever study of its kind, the effect of vermicompost, derived solely from an allelopathic weed, on the germination, growth, and yield of a botanical species, has been carried out. In test plots, the soil was treated with the vermicompost of lantana (Lantana camara) at the rates of 5, 7.5, and 10 t ha(-1), and cluster bean (Cyamopsis tetragonoloba) was grown on it. The performance of these systems was compared with the systems in which the soil was fortified with inorganic fertilizers (IFs) in concentrations equivalent to those present in the respective vermicompost (VC) treatments. Additionally, a set of control was studied in which the soil was used without fortification by either VC or IF. It was seen that up to 51.5 % greater germination success occurred in the VC treatments compared to controls. VC also supported better plant growth in terms of stem diameter, shoot length, shoot mass, number of leaves, and leaf pigments. The positive impact extended up to fruit yield. In addition, vermicast application enhanced root nodule formation, reduced disease incidence, and allowed for a smaller number of stunted plants. The results indicate that allelopathic ingredients of lantana seem to have been totally eliminated during the course of its vermicomposting and that lantana vermicompost has the potential to support germination, growth, and fruit yield better than equivalent quantities of IFs.