ABSTRACT
Expression of Concern for 'Statistical optimization of photo-induced biofabrication of silver nanoparticles using the cell extract of Oscillatoria limnetica: insight on characterization and antioxidant potentiality' by Rasha A. Abo-Elmagd et al., RSC Adv., 2020, 10, 44232-44246, DOI: https://doi.org/10.1039/D0RA08206F.
ABSTRACT
Introduction: Superficial infrapatellar bursitis may be traumatic, inflammatory, or infectious. It rarely attains massive dimensions. Open excision can be performed as a one-stage or a two-stage procedure, and endoscopy can be an alternative. We report a chronic, unusually massive, hemorrhagic infrapatellar bursa which we resected totally in a single stage procedure with no significant complications. Case Report: A 65-year-old male farmer presented with a chronic massive swelling in front of the right knee which first appeared 4 years earlier and had no effect on his general condition. The mass was excised in a one-stage procedure and it was found to be a hemorrhagic bursitis. The wound healed uneventfully and, on follow-up 7 weeks later, the patient was able to perform full flexion and extension of the knee. Conclusion: Two-stage procedures for excision are effective, but one-stage procedures are equally effective in chronic cases, provided the dissection is performed meticulously and atraumatically.
ABSTRACT
Rhodotorula yeasts which are known as carotenogenic yeasts have a great industrial value due to their ability to produce carotenoids. In particular, the isolated yeast Rhodotorula sp. (strain ATL72) has been reported to be a promising producer of high concentrations of carotenoids. A combination of central composite design (CCD) and Plackett-Burman (PB) design was used to optimize carotenoids produced by this yeast. The optimum production of carotenoids was completed when the yeast was grown in a production medium composed of 3.7 g/L malt extract, 7.7 g/L fructose, 9 g/L urea, 35 g/L NaCl, and 1 g/L yeast extract at 27.5 °C, pH 6.7, and 180 rpm. Two batch runs in 1 L and 7 L bioreactors were conducted which increased the productivity of carotenoid concentration from 21.5 mg/L after 98 h of incubation at the level of the shake flask to 229.9 mg/L after 47 h of incubation at the level of 7 L bioreactor. The carotenoid pigment was extracted in dimethylsulfoxide (DMSO), acetone, petroleum ether, and sodium chloride, and subsequently identified and characterized using UV-visible scanning, thin layer chromatography, and gas chromatography/mass spectrometry.
ABSTRACT
Silver nanoparticles were successfully fabricated through a very simple, rapid, one-step photo-induced green approach. The formation of silver nanoparticles was accomplished using the bioactive compounds in the aqueous extract of fresh Oscillatoria limnetica biomass, which acted as a reducing and capping agent at the same time. The biosynthesis of Oscillatoria-silver nanoparticles (O-AgNPs) was investigated under the influence of different light intensities 57.75, 75.90 and 1276.51 µmol m-2 s-1 (bright sunlight). UV-Vis (UV) and Fourier transform infrared (FT-IR) spectroscopy were applied to approve the synthesis of AgNPs. Further, the synthesis process under the exposure to sunlight was adjusted via utilizing one factor at a time, and 0.5 mM AgNO3 concentration, 5 mL O. limnetica solution, pH 6.7 and 30 min sunlight (1276.51 µmol m-2 s-1) were applied. Furthermore, the central composite design (CCD) was applied to boost the biosynthesis process of O-AgNPs (manufactured at light intensity 75.90 µmol m-2 s-1). The maximum production of O-AgNPs was attained with 4 detected variables: initial pH level (6.7), AgNO3 concentration (0.3 mM), O. limnetica extract concentration (3.50 mL) and incubation time (48 h). Moreover, TEM, in addition to SEM, images exposed that the biosynthesized AgNPs were quasi-spherical in shape with a small monodisperse nature, and the size range was between 6.98-23.48 nm in the case of light-induced synthesis (75.90 µmol m-2 s-1) and 11.58-22.31 nm with sunlight (1276.51 µmol m-2 s-1).
