ABSTRACT
Surgical site infections (SSIs) in developing countries have been linked to inadequate availability of sterilising equipment. Existing autoclaves are mostly unaffordable by rural healthcare practitioners, and when they managed to procure them, the electricity supply to power the autoclaves is epileptic. The solar-powered autoclave alternatives are too bulky with a very high initial cost. Hence, low-cost biofuel-powered autoclave becomes an attractive option, and this study sought to present the design, development and clinical evaluation of the device performance. With the global drive for the adoption of green energy, biofuel will not only reduce greenhouse gas emission but also provide revenue for local producers and reduce biomass associated health complications. The theoretical energy requirement for the sterilisation process was calculated. The standard pressure and temperature needed for sterilisation were tested to be 121 °C and 15 psi. The device was also clinically tested with Staphylococcus aureus bacteria obtained from the Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital using Brain heart Infusion Broth, MacConkey and Blood agar as cultured media. No bacteria growth was observed when the samples containing the bacteria colony were autoclaved by the designed autoclave and incubated at 37 °C for 2 d. Hence, the device met the mechanical and biological validation standards for effective sterilisation.