Expression of AMHR2 and C-KIT in cervical lesions in Uyghur Women of Xinjiang, China.

BACKGROUND: Cervical cancer is one of the most common malignant tumors in women. Anti-Müllerian hormone receptor 2 (AMHR2) and C-Kit were two members of protein kinase which were reported increased in some cancers like ovarian carcinoma and breast cancer. The present study aimed to assess the expression of AMHR2 and c-Kit in cervical cancer of different differentiated degrees as well as in cervicitis sections. METHODS: All the lesions were collected randomly during clinical observations in hospitals located in Xinjiang, China. Polymerase chain reaction (PCR) and immunohistochemical staining were used to detect AMHR2 and c-Kit expression in cervical samples from women who had been infected with human papilloma virus (HPV)16. The expression rate was compared between cervical cancer of well, moderately and poorly differentiated and cervicitis. RESULTS: The average age of the patients was 45 years; ranged from 23 to 80. For AMHR2, all 17 cervicitis samples ranged from (++) to (++++), while cervical cancer showed 11 (+), 9 (++), 15 (+++),9 (++++), and 8 (-), which showed AMHR2 expression was lessen with the poorer of differentiation degree of cervical cancer (P < .05). For c-Kit, 18 cervicitis samples mainly expressed as (-) with none showed (+++) or (++++), while cervical cancer samples showed 7 (-), 6 (+), 1 (++), 2 (+++), and 8 (++++), which indicated c-Kit's expression increased with the reduction of cervical cancer's differentiation degree (P < .05). CONCLUSION: AMHR2 might have some correlation with self defense of our body, while c-Kit might link with the potential invasive capacity of cervical cancer.

Changes in mucin 1 expression in a rat model of allergic airway inflammation.

PURPOSE: To explore the distribution and expressional changes of mucin 1 (Muc1) in airway of rats with allergic airway inflammation. MATERIALS AND METHODS: Ovalbumin (OVA) was used to induce acute allergic inflammation in male Wistar rats. The distributions and expressions of Muc1 in lungs of normal and model rats were assessed by immunohistochemical staining and western blotting, respectively. RESULTS: Immunohistochemical staining showed that Muc1 distributed in airway epithelial cells with ciliates, but not those nonciliated cells. Mucin 1 protein expression in the lung was increased during the development of allergic airway inflammation when compared with the normal rats. CONCLUSION: Mucin 1 distributes in the airway epithelial cells with ciliates and the expressional increase of Muc1 in lung may imply its functions on allergic inflammatory episodes.

Elevated Expression of Ox2R in Cervical Cancers and Placentas of Uyghur Women in Xinjiang, China

Objective:Cervical cancer is one of the major causes of mortality of Uyghur women in Xinjiang, China. Although increased expression of orexin receptor (OxR), known to be strongly expressed in human placenta, has a proven relation to some cancers , there have been few studies of cervical cancer. Thus, we explored this question by evaluating the expression of orexin receptor as a biomarker for screening early stage of cervical cancer in Uyghur women with highest occurrence rate of cervical cancer in China. Study Design: We used polymerase chain reaction (PCR) and immunohistochemical staining to determine the expression of both Ox1R and Ox2R in cervical cancer and cervicitis biopsies collected from Uyghur women infected with human papilloma virus (HPV)16. The expression rate was compared between cervical cancers of low, intermediate and high differentiation and cervicitis. Results:Although there was no significant difference in the expression rate of Ox1R between groups, Ox2R was significantly overexpressed in cervical cancer patients when compared to the cervicitis group. Ox1R was negative in normal human placenta while Ox2R was positive. Conclusions: While expression of Ox1R had no correlation with invasion or metastatic potential, Ox2R demonstrated elevation in cervical cancer with heterogeneity in groups with different metastatic potential, in the human placenta as well, implying that it might serve as an indicator of invasive capacity along with other indices.

Expression of Galectin-7 in squamous cell carcinoma of the cervix and its association with HPV 16 infection / 临床与实验病理学杂志

Purpose To study the expression of Galectin-7 in squamous carcinoma of the cervix and its correlation with HPV 16 infec-tion in Uyghur and Han women. Methods The expression of Galectin-7 in paraffin-embedded cervical tissues collected from Uyghur women including 21 patients with cervicitis and 49 patients with squamous carcinoma of the cervix and Han women including 25 patients with cervicitis and 29 patients with squamous cervical cancer was evaluated by immunohistochemical SP method. The infection of HPV 16 was evaluated by PCR amplification of HPV DNA from the corresponding cervical tissue samples. The expression of levels of Galec-tin-7 in cervical tissues was analyzed according to the corresponding integrated optical density ( IOD) value of Galectin-7 in cervical le-sions with professional image analysis software Image-Pro Plus (IPP) 6. 0. Results PCR detection of HPV 16 in Uyghur patients from Kashghar showed that 20 patients were positive and 29 cases were negative for HPV 16 in squamous cervical cancer patients whereas in patients with cervicitis, only 5 cases were positive, 16 cases were negative for HPV 16. Furthermore the detection of HPV 16 in Han patients showed that 2 cases were positive and 27 cases was negative in patients with squamous cervical cancer patients, and all the ca-ses were negative for HPV 16 in patients with cervicitis. The expression of Galectin-7 in squamous cervical cancer tissues was signifi-cantly lower than that in the cervicitis patients (P<0. 05). The expression of Galectin-7 in HPV 16 positive squamous cervical cancer patients was significantly lower than that in HPV 16 negative patients ( P<0. 05 ) . There was no obvious correlation between the ex-pression levels of Galectin-7 in squamous cervical cancer patients with the differentiation degree and disease oneset age both in Uyghur and Han patients. Conclusion Compared with cervicitis tissues, Galectin-7 expression in squamous cervical cancer is down-regulated in Uyghur women from Kashghar region implying that the expression of Galectin-7 may play a certain role in the occurrence and develop-ment of squamous cervical cancer, and its detection has a certain reference value for the diagnosis of cervical cancer for Uyghur women.

Expression of p16 in cervical cancer in Urghur women and its correlation with HPV1618 infection / 西安交通大学学报(医学版)

0.05).There were significant differences in the expression of p16 proteins in cervical cancers (97.50%) compared to the expression of those in normal cervical tissues (2.94%) both in Urghur and Han nationalities (P0.05).Furthermore,there was a positive correlation between the expression of p16 and infection of HPV16 (r=0.603,P