Vascularized osseous flaps and assessing their bipartate perfusion pattern via intraoperative fluorescence angiography.

BACKGROUND: Large segmental bone and composite tissue defects often require vascularized osseous flaps for definitive reconstruction. However, failed osseous flaps due to inadequate perfusion can lead to significant morbidity. Utilization of indocyanine green (ICG) fluorescence angiography has been previously shown to reliably assess soft tissue perfusion. Our group will outline the application of this useful intraoperative tool in evaluating the perfusion of vascularized osseous flaps. METHODS: A retrospective review was performed to identify those osseous and/or osteocutaneous bone flaps, where ICG angiography was employed. Data analyzed included flap types, success and failure rates, and perfusion-related complications. All osseous flaps were evaluated by ICG angiography to confirm periosteal and endosteal perfusion. RESULTS: Overall 16 osseous free flaps utilizing intraoperative ICG angiography to assess vascularized osseous constructs were performed over a 3-year period. The flaps consisted of the following: nine osteocutaneous fibulas, two osseous-only fibulas, two scapular/parascapular with scapula bone, two quadricep-based muscle flaps, containing a vascularized femoral bone component, and one osteocutaneous fibula revision. All flap reconstructions were successful with the only perfusion-related complication being a case of delayed partial skin flap loss. CONCLUSIONS: Intraoperative fluorescence angiography is a useful adjunctive tool that can aid in flap design through angiosome mapping and can also assess flap perfusion, vascular pedicle flow, tissue perfusion before flap harvest, and flap perfusion after flap inset. Our group has successfully extended the application of this intraoperative tool to assess vascularized osseous flaps in an effort to reduce adverse outcomes related to preventable perfusion-related complications.

Abdominal compartment syndrome: current problems and new strategies.

BACKGROUND: Abdominal compartment syndrome (ACS) is a difficult entity with two main problems during its course: (1) survival of the patient during the early period and (2) closure of the open wounds during the late period. In this study we evaluated the decision to decompress according to the level of intraabdominal pressure (IAP) and analysis of any recurrent or persistent increase in IAP. METHODS: A prospective study was undertaken on 119 patients with increased IAP. The IAP was measured daily by obtaining the bladder pressure. Patients were monitored via a central venous line; and vital signs, arterial blood gases, the Acute Physiology, Age, and Chronic Health Evaluation II (APACHE II) score, and abbreviated mental tests were recorded. The suggestions of Meldrum et al. were taken as a guideline during the treatment. The sensitivity and specifity of IAP and APACHE II scores for different cutoff values were calculated using the receiver operating characteristic curve. RESULTS: Hospital mortality was 33.6%, which increased with co-morbidities (p = 0.03). A cutoff value for IAP of 23 mmHg was considered an optimal point predicting mortality. The IAP within the first 3 days for patients who died was higher than the cutoff value. For patients with IAP of 15 to 25 mmHg, nonsurgical therapy increased the rate of mortality (odds ratio 5.2, 95% confidence interval 1.0-27.7; p = 0.03). CONCLUSIONS: In patients with ACS emergency, it is recommended that decompressive laparotomy to be performed even if the IAP falls below 25 mmHg. For patients with IAP levels higher than 25 mmHg, the IAP should be meticulously brought below the cutoff level during the postoperative period.

Anti-TGF-beta2 antibody therapy inhibits postoperative resynostosis in craniosynostotic rabbits.

BACKGROUND: Postoperative resynostosis is a common clinical finding. It has been suggested that an overexpression of transforming growth factor (TGF)-beta2 may be related to craniosynostosis and may contribute to postoperative resynostosis. Interference with TGF-beta2 function with the use of neutralizing antibodies may inhibit resynostosis. The present study was designed to test this hypothesis. METHODS: New Zealand White rabbits with bilateral coronal suture synostosis were used as suturectomy controls (group 1, n = 9) or given suturectomy with nonspecific, control immunoglobulin G antibody (group 2, n = 9) or suturectomy with anti-TGF-beta2 antibody (group 3, n = 11). At 10 days of age, a 3 x 15-mm coronal suturectomy was performed. The sites in groups 2 and 3 were immediately filled with 0.1 cc of a slowly resorbing collagen gel mixed with either immunoglobulin G (100 mug per suture) or anti-TGF-beta2 (100 mug per suture). Three-dimensional computed tomography scan reconstructions of the defects were obtained at 10, 25, 42, and 84 days of age, and the sutures were harvested for histomorphometric analysis. RESULTS: Computed tomography scan data revealed that the suturectomy sites treated with anti-TGF-beta2 showed significantly (p < 0.05) greater areas through 84 days of age compared with controls. Histomorphometry also showed that suturectomy sites treated with anti-TGF-beta2 had patent suturectomy sites and more fibrous tissue in the defects compared with sites in control rabbits and had significantly (p < 0.001) less new bone area (by approximately 215 percent) in the suturectomy site. CONCLUSIONS: These data support the initial hypothesis that interference with TGF-beta2 function inhibited postoperative resynostosis in this rabbit model. They also suggest that this biologically based therapy may be a potential surgical adjunct to retard postoperative resynostosis in infants with craniosynostosis.

The use of external skeletal fixation to facilitate the surgical release of wrist flexion and thumb web space contractures.

PURPOSE: To evaluate the use of external fixation as a splint to keep the hand in the desired position after simultaneous joint and soft-tissue release in a single stage for treatment of first web space and wrist contractures. METHODS: Six first web space adduction and 7 wrist flexion contractures were released surgically. All patients had prior unsuccessful surgery. After surgical release of the contracture and capsulotomy, external fixator pins were inserted into the first and second metacarpals to maintain thumb abduction and into the radius and second metacarpal to maintain wrist extension, followed by skin grafting. External fixation was followed by splinting. Results were based on persistence of contracture release, rate of complications, and functional outcome. RESULTS: Before surgery, the thumbs were contracted at an average of 0 degrees of adduction with no range of motion, and wrists were contracted between 85 degrees to 100 degrees of flexion. The duration of contracture and number of prior surgeries did not influence the amount of release obtained during the surgery. After 7 months to 7 years of follow-up of first web space contractures, the thumb was in an average of 55 degrees of palmar abduction. Patients were able to oppose and fully adduct. At long-term follow-up examinations of the wrist contractures, patients had the wrist in the neutral position (0 degrees) in the resting state, with active extension ranging between 5 degrees and 15 degrees and flexion ranging between 35 degrees and 45 degrees . In 1 patient the wrist was at 45 degrees of flexion in the resting state with an arc of motion of 20 degrees . In 1 patient the wrist contracted back to the preoperative position, requiring another surgery. All patients experienced increased activity and improvement in grasping objects at 6-month follow-up evaluations. Complications included 3 pin site infections, 1 severe discomfort after 6 weeks, and 1 median nerve compression. All were treated successfully. CONCLUSIONS: External fixation can be used to maintain position in cases of first web space and wrist flexion contractures after surgical release, especially in patients for whom standard methods have failed. It is safe, efficacious, and well tolerated. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IV.

Correction of unilateral coronal synostosis leads to resolution of mandibular asymmetry in rabbits.

Mandibular dysmorphology in unilateral coronal synostosis has been recognized clinically. In patients with unilateral coronal synostosis, the chin point deviates away from the affected side. To investigate whether this mandibular asymmetry resolves after correction of unilateral coronal synostosis, familial nonsyndromic rabbits were used. Rabbits with unilateral coronal synostosis that underwent "correction" with resection of the affected suture were compared with "uncorrected" rabbits with unilateral coronal synostosis and normal, wild-type rabbits (n = 36; three equal groups of 12). Serial lateral cephalograms obtained at 10, 25, 42, and 84 days showed no asymmetries in wild-type rabbits and progressive asymmetries in the ramal height and mandibular length in uncorrected unilateral coronal synostosis rabbits. However, in corrected unilateral coronal synostosis rabbits, existing asymmetries at 10 and 25 days improved by 42 days and were not seen by maturity, at 84 days. In dry, mature, mandibular specimens, wild-type rabbits showed equal side-to-side measurements and uncorrected unilateral coronal synostosis rabbits showed the following on the affected side: longer ramal height (15 percent), shorter ramal width (13 percent), longer body height (10 percent), and shorter body width (13 percent). By contrast, the corrected unilateral coronal synostosis specimens showed no side-to-side differences in 10 of 11. There were no asymmetries in condylar shape or condylar volume in any of the three groups. Cranial base measurements showed asymmetries of the uncorrected unilateral coronal synostosis specimens that were consistent with an anteriorly positioned glenoid fossa on the affected side. However, only one of 11 corrected unilateral coronal synostosis specimens showed similar cranial base asymmetries. The data showed that mandibular asymmetries in nonsyndromic, familial rabbits with unilateral coronal synostosis are progressive with growth but improve after correction of synostosis.

Panniculectomy as an adjuvant to bariatric surgery.

A large hanging panniculus can cause problems such as intertrigo, chronic infection, and immobility. Many patients undergoing weight reduction surgery can benefit from panniculectomy either done concomitantly with bariatric surgery or later after significant weight reduction. Over the last 5 years we performed 123 panniculectomies on patients (34 males, 89 females; mean age 44.5 +/- 10.3 years) undergoing bariatric surgery. The panniculectomy was either done at the same time as the bariatric surgery in 21 patients or after a time period of 17 +/- 11 months in 102 patients. The prebariatric surgery weight ranged from 107 to 341 kg (mean: 168.6 +/- 47.2 kg) with a mean body mass index (BMI) of 59 +/- 14 kg/m. After the bariatric surgery the patients had an average weight loss of 57.6 +/- 27 kg. The prepanniculectomy weight was 121.9 +/- 39.3 kg (BMI = 43.1 +/- 12.4 kg/m) for the patients who had the panniculectomy after the bariatric surgery. Ninety-two percent of the patients had multiple comorbidities. The weight of the panniculectomy specimen ranged from 4 to 54 kg. Any abdominal wall hernias (35.4% incisional and 8.9% umbilical) were fixed during the panniculectomy. Overall, patients who had panniculectomy simultaneously with the bariatric surgery had more complications than patients who had panniculectomy after their bariatric surgery. The wound infections were 48% versus 16% and respiratory distress was 24% versus 0%, respectively. The skin necrosis was 10% versus 6%, dehiscence was 33% versus 13%, and hematoma formation was 10% versus 2%, respectively. Overall, the patients had good outcomes, with 3 postoperative deaths in the group with panniculectomy at the same time of bariatric surgery. An interval of weight loss prior to the procedure makes this procedure safer and more effective.

Reconstruction of difficult wounds with tissue-expanded free flaps.

Tissue expansion of free flaps before transfer have been used to increase the size of the transferred tissue and to allow primary closure of the donor site. This is especially important in burns when there is a lack of healthy tissue, and in children when there is a relative lack of tissue. In this study the authors present their 17-year experience with tissue-expanded free flaps. Between 1985 and 2002, 14 cases of tissue expansion of free flaps before transfer were performed at the authors' institution. The indications for vascularized tissue were 9 facial defects (8 severe burns and 1 neurofibromatosis), 3 large lumbosacral ulcers resulting from spina bifida, 1 large vulvoperineal defect resulting from resection for Chron disease, and 1 large circumferential tissue defect at the lower extremity resulting from tumor extirpation. The flaps were 6 radial forearm, 4 parascapular, and 4 lateral arm free flaps. Tissue expanders were placed under the subcutaneous plane between the deep fascia and the muscle layer, preserving the pedicle architecture and integrity. The size of the tissue expander ranged from 250 to 700 mL and expansions were done either weekly or biweekly for a 10- to 20-week period. The patients were followed for 1 to 17 years. The outcome of the study was based on donor and recipient site morbidity, rate of complications, patient satisfaction, and long-term outcome of the donor and recipient sites. There was no failure in any of the transferred tissue at the recipient site and no tissue loss. Three patients had transient congestion of the free flaps, which resolved without any intervention. In all patients the donor area was closed primarily. One black patient had hypertrophic scar formation at the radial forearm donor site requiring reexpansion and primary closure. In other patients the scar was acceptable. One patient had an infection at the tissue expander site that was treated with removal of the implant and transfer of the free flap to the recipient defect the following day. In 4 patients with facial burns, posttransfer tissue expansion of the free flap at the recipient site was performed for minor revisions at later dates. All patients had good outcomes and were satisfied with the procedure. Preexpansion of free flaps is an effective but underused method. It is safe in terms of complications, results in a better appearing donor site scar, and allows transfer of larger amounts of tissue in patients with tissue deficiency.