Scattering/Fluorescence Dual-Mode Imaging in MnO2-Coated Silicon Nanospheres for Cancer Cell Detection.

A tumor microenvironment (TME)-responsive nanoprobe composed of a fluorescent dye-decorated silicon (Si) nanosphere core and a thin MnO2 shell is proposed for simple and intelligent detection of cancer cells. The Si nanosphere core with diameters of 100-200 nm provides environment-independent Mie scattering imaging, while, simultaneously, the MnO2 shell provides the capability to switch the on/off state of the dye fluorescence reacted to the glutathione (GSH) and/or H2O2 levels in a cancer cell. Si-MnO2 core-shell nanosphere probes are fabricated in a solution-based process from crystalline Si nanosphere cores. The fluorescence switching under exposure to GSH is demonstrated, and the mechanism is discussed based on detailed optical characterizations including single-particle spectroscopy. Different types of human cells are incubated with the nanoprobes, and a proof of concept experiment is performed. From the combination of the robust scattering images and GSH- and H2O2-sensitive fluorescence images, the feasibility of cancer cell detection by the multimodal nanoprobes is demonstrated.

Granular vibration pumping system for handling and characterizing particulate materials.

A unique setup for a granular vibration pumping system was developed to ease the installation into a wide variety of granular handling processes and provide flexible options for granular physics research. Although the granular vibration pumping system can notably lift granular materials with an oscillating pipe, the climbing mechanism and related granular physics are yet to be clarified thoroughly. The new setup employs an eccentric cam mechanism as the excitation source, a linear system with dust tolerance, and a recording system, making it simple, compact, and adaptable for extending experiments. The excitation mechanism generated a clear sinusoidal vibration in the pipe, realizing better reproducibility of the climbing motions of glass beads. Moreover, the compact design facilitates the close placement of multiple pipes vibrating individually, which affects the transport performance. In addition, various types of sample cells that store particles and the imaging system allow for the detailed observation of particle motions in the pipes and even the sample cells. This developed system provides easy and accurate tuning of the existing parameters of the granular vibration pumping system, as well as new options for a further understanding of granular physics.

Photoluminescence from FRET pairs coupled with Mie-resonant silicon nanospheres.

Optically resonant nanoparticles decorated with donor-acceptor molecular pairs have been attracting attention for applications as nanoprobes in bioimaging and biosensing. We produced composite nanoparticles composed of donor-acceptor molecular pairs and silicon nanospheres (Si NSs) with diameters of 100-200 nm exhibiting Mie resonances in the visible range and studied the effect of Mie resonances on their photoluminescence properties. We showed that the photoluminescence spectra are strongly modified by Mie resonances and the spectral shape is controlled in a wide range by the Si NS size; by controlling the size, we can achieve the photoluminescence maximum from that of a donor molecule to that of an acceptor molecule almost continuously. From the photoluminescence decay properties in combination with theoretical calculations, we showed that the observed strong modification of the spectral shape is mainly due to the Purcell effect on donor and acceptor molecules, and the effect of Mie resonances on the Förster resonance energy transfer (FRET) rate is relatively small. We also showed that because of the large Purcell effect and the small FRET rate enhancement, Mie resonances decrease the FRET efficiency.

Fluorophore-Decorated Mie Resonant Silicon Nanosphere for Scattering/Fluorescence Dual-Mode Imaging.

Inorganic nanoparticles with multiple functions have been attracting attention as multimodal nanoprobes in bioimaging, biomolecule detection, and medical diagnosis and treatment. A drawback of conventional metallic nanoparticle-based nanoprobes is the Ohmic losses that lead to fluorescence quenching of attached molecules and local heating under light irradiation. Here, metal-free nanoprobes capable of scattering/fluorescence dual-mode imaging are developed. The nanoprobes are composed of a silicon nanosphere core having efficient Mie scattering in the visible to near infrared range and a fluorophore doped silica shell. The dark-field scattering and photoluminescence images/spectra for nanoprobes made from different size silicon nanospheres and different kinds of fluorophores are studied by single particle spectroscopy. The fluorescence spectra are strongly modified by the Mie modes of a silicon nanosphere core. By comparing scattering and fluorescence spectra and calculated Purcell factors, the fluorescence enhancement factor is quantitatively discussed. In vitro scattering/fluorescence imaging studies on human cancer cells demonstrate that the developed nanoparticles work as scattering/fluorescence dual-mode imaging nanoprobes.

Magnetic excitation of a granular gas as a bulk thermostat.

A thermostat utilizing a varying magnetic field has been developed to agitate soft ferromagnetic particles in microgravity platforms for an investigation of an energy-dissipative granular gas. Although the method has experimentally realized a reasonably homogeneous spatial distribution of particles, the physics behind the magnetically excited particles has not been understood. Therefore, a numerical calculation based on the discrete element method is developed in this paper to explain the realization of homogeneously distributed particles. The calculation method allows considering inelastic and magnetic interactions between particles and tracking the motions due to those interactions during the excitation of the granular gas. The calculation results, compared with the experimental result, show that magnetic interactions between particles, a time-domain variation of magnetic-excitation directions, and random collisions of particles between each magnetic excitation contribute to distribute particles homogeneously.

Magnetically excited granular matter in low gravity.

Due to the undesired impact of gravity, experimental studies of energy-dissipative gaseous systems are difficult to carry out on ground. In the past several years, we developed a series of experimental devices suitable for various kinds of microgravity platforms. The central idea adopted in our devices is to use long-range magnetic forces to excite all the particles within the system. Through the development of our devices, different component configurations, excitation protocols, and image-capturing methods have been tried and optimized to achieve best excitation and the maximum capability for data analysis.