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1.
Int Wound J ; 15(1): 106-113, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29052352

ABSTRACT

The purpose of this study was to investigate incidence rates and levels of microbial contamination in open-but-unused portions of wound dressings stored in home settings. Portions of wound dressings were collected at up to four home visits for 104 clients undertaking wound management within their home. A control sample and stored sample was collected on each home visit and sent for pathology testing to identify levels of microbial contamination. The stored open-but-unused wound dressings were managed according to a written protocol. Of the tested samples (n = 776), 6% of control samples and 7% of test samples had microbial contamination. From regression analysis, the stored samples were more likely to have microbial contamination than control samples, but results were not statistically significant. In comparing occasions of storage and handling across four home visits, after adjusting for sample group and dressing type, none of the home visit occasion regressions were statistically significant. In conclusion, storage of open-but-unused portions of wound dressings kept in home settings does not appear to increase the rate of microbial contamination compared to newly opened wound dressings.


Subject(s)
Bandages/microbiology , Colony Count, Microbial , Equipment Contamination/statistics & numerical data , Home Care Services/statistics & numerical data , Humans , South Australia
2.
J Infect Dis ; 190(11): 1913-8, 2004 Dec 01.
Article in English | MEDLINE | ID: mdl-15529254

ABSTRACT

This study, conducted during 2001-2003, undertook the screening of patients with acute infectious respiratory-tract disease. A random selection of positive specimens was used for sequencing studies of the human metapneumovirus (hMPV) nucleoprotein gene and the phosphoprotein (P) gene. Australian and international sequences were compared, and a global classification scheme was developed. The hMPV P gene was an ideal molecular target for the detection and genotyping of hMPV. The region contained conserved sequences for reverse-transcriptase-polymerase chain-reaction primers and adequate variability to permit the accurate genotyping of the virus into 2 main lineages and 4 sublineages. Establishing viral identity is essential for the linking of genotype and disease severity.


Subject(s)
Genes, Viral , Metapneumovirus/genetics , Paramyxoviridae Infections/epidemiology , Phosphoproteins/genetics , Respiratory Tract Infections/epidemiology , Acute Disease , Australia/epidemiology , Child , Family Practice , Female , Genotype , Hospitals , Humans , Male , Metapneumovirus/classification , Molecular Epidemiology , Molecular Sequence Data , Nucleoproteins/genetics , Phylogeny , Respiratory Tract Infections/virology , Sequence Alignment
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