ABSTRACT
Scaffold design is one of the three most essential parts of tissue engineering. Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) have been used in clinics and regenerative medicine for years. However, the temporal release of their growth factors limits their efficacy in tissue engineering. In the present study, we planned to synthesize nanofibrous scaffolds with the incorporation of PRP and PRF by electrospinning method to evaluate the effect of the release of PRP and PRF growth factors on osteogenic gene expression, calcification, proliferation, and cell adhesion of human bone marrow mesenchymal stem cell (h-BMSC) as they are part of scaffold structures. Therefore, we combined PRP/PRF, derived from the centrifugation of whole blood, with gelatin and Polycaprolactone (PCL) and produced nanofibrous electrospun PCL/Gel/PRP and PCL/Gel/PRF scaffolds. Three groups of scaffolds were fabricated, and h-BMSCs were seeded on them: (1) PCL/Gel; (2) PCL/Gel/PRP; (3) PCL/Gel/PRF. MTS assay was performed to assess cell proliferation and adhesion, and alizarin red staining confirmed the formation of bone minerals during the experiment. The result indicated that PCL/Gel did not have any better outcomes than the PRP and PRF group in any study variants after the first day of the experiment. PCL/gelatin/PRF was more successful regarding cell proliferation and adhesion. Although PCL/gelatin/PRP showed more promising results on the last day of the experiment in mineralization and osteogenic gene expression, except RUNX2, in which the difference with PCL/gelatin/PRF group was not significant.
Subject(s)
Cell Adhesion , Cell Proliferation , Gelatin , Mesenchymal Stem Cells , Osteogenesis , Platelet-Rich Fibrin , Platelet-Rich Plasma , Polyesters , Tissue Scaffolds , Humans , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Gelatin/chemistry , Tissue Scaffolds/chemistry , Polyesters/chemistry , Platelet-Rich Plasma/metabolism , Platelet-Rich Plasma/chemistry , Cell Proliferation/drug effects , Cell Adhesion/drug effects , Platelet-Rich Fibrin/chemistry , Platelet-Rich Fibrin/metabolism , Cells, Cultured , Tissue Engineering/methods , Nanofibers/chemistryABSTRACT
OBJECTIVE: The aim of this study was to review the selection criteria of resin cements for different types of partial coverage restorations (PCRs) and investigate if the type of restorations or restorative materials affect the type of selected resin cement. MATERIALS AND METHODS: An electronic search (1991-2023) was performed in PubMed, Medline, Scopus, and Google Scholar databases by combinations of related keywords. RESULTS: A total of 68 articles were included to review the selection criteria based on the advantages, disadvantages, indications, and performance of resin cements for different types of PCRs. CONCLUSIONS: The survival and success of PCRs are largely affected by appropriate cement selection. Self-curing and dual-curing resin cements have been recommended for the cementation of metallic PCRs. The PCRs fabricated from thin, translucent, and low-strength ceramics could be adhesively bonded by light-cure conventional resin cements. Self-etching and self-adhesive cements, especially dual-cure types, are not generally indicated for laminate veneers.
Subject(s)
Ceramics , Resin Cements , Resin Cements/therapeutic use , Dental Cements/therapeutic use , CementationABSTRACT
Background: To consciously select an appropriate dental cement for each type of intracanal post. Materials and Methods: An electronic search was carried out (1970 to 2020) through Medline, PubMed, Scopus, and Google Scholar. The following keywords were searched in title, abstract, or keywords with different combinations: endodontically treated tooth, root canal therapy, dental posts, post and core, prefabricated posts, custom posts, dental cement, resin cements, cement selection, cement, and adhesive resin cement. Results: Evaluating full texts, 146 articles were selected to review the types of posts and cements, selection criteria of appropriate cement for each type of post, and compare the results obtained by different cements. Conclusion: Dental cements affect the survival rate, durability, and success rate of postbased treatments. Considering special characteristics and application of each type of intracanal post, conscious selection of cement is an important determining factor in long-lasting success. Choosing an appropriate cement has a key role in success and durability of dowel posts-based fixed restorations.
