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1.
Asian J Neurosurg ; 18(4): 751-760, 2023 Dec.
Article in English | MEDLINE | ID: mdl-38161609

ABSTRACT

Purpose The aim of this study was to evaluate the role of permeability surface area product in grading brain gliomas using computed tomography (CT) perfusion Materials and Methods CT perfusion was performed on 33 patients with brain glioma diagnosed on magnetic resonance imaging. Of these, 19 had high-grade glioma and 14 had low-grade glioma on histopathological follow-up. CT perfusion values were obtained and first compared between the tumor region and normal brain parenchyma. Then the relative values of perfusion parameters were compared between high- and low-grade gliomas. Cut-off values, sensitivity, specificity, and strength of agreement for each parameter were calculated and compared subsequently. A conjoint factor (permeability surface area product + cerebral blood volume) was also evaluated since permeability surface area product and cerebral blood volume are considered complimentary factors for tumor vascularity. Results All five perfusion parameters namely permeability surface area product, cerebral blood volume, cerebral blood flow, mean transit time, and time to peak were found significantly higher in the tumor region than normal brain parenchyma. Among these perfusion parameters, only relative permeability surface area product and relative cerebral blood volume were found significant in differentiating high- and low-grade glioma. Moreover, relative permeability surface area product was significantly better than all other perfusion parameters with highest sensitivity and specificity (97.74 and 100%, respectively, at a cut-off of 9.0065). Relative permeability surface area product had a very good agreement with the histopathology grade. The conjoint factor did not yield any significant diagnostic advantage over permeability surface area product. Conclusion Relative permeability surface area product and relative cerebral blood volume were helpful in differentiating high- and low-grade glioma; however, relative permeability surface area product was significantly better than all other perfusion parameters. Grading brain gliomas using relative permeability surface area product can add crucial value in their management and prognostication; hence, it should be evaluated in the routine CT perfusion imaging protocol.

2.
Transl Neurodegener ; 11(1): 48, 2022 11 07.
Article in English | MEDLINE | ID: mdl-36345044

ABSTRACT

Lipids, defined by low solubility in water and high solubility in nonpolar solvents, can be classified into fatty acids, glycerolipids, glycerophospholipids, sphingolipids, and sterols. Lipids not only regulate integrity and fluidity of biological membranes, but also serve as energy storage and bioactive molecules for signaling. Causal mutations in SPTLC1 (serine palmitoyltransferase long chain subunit 1) gene within the lipogenic pathway have been identified in amyotrophic lateral sclerosis (ALS), a paralytic and fatal motor neuron disease. Furthermore, lipid dysmetabolism within the central nervous system and circulation is associated with ALS. Here, we aim to delineate the diverse roles of different lipid classes and understand how lipid dysmetabolism may contribute to ALS pathogenesis. Among the different lipids, accumulation of ceramides, arachidonic acid, and lysophosphatidylcholine is commonly emerging  as detrimental to motor neurons. We end with exploring the potential ALS therapeutics by reducing these toxic lipids.


Subject(s)
Amyotrophic Lateral Sclerosis , Motor Neuron Disease , Humans , Amyotrophic Lateral Sclerosis/genetics , Amyotrophic Lateral Sclerosis/therapy , Amyotrophic Lateral Sclerosis/metabolism , Translational Science, Biomedical , Motor Neurons/pathology , Motor Neuron Disease/metabolism , Ceramides/metabolism
3.
Indian Dermatol Online J ; 13(2): 270-271, 2022.
Article in English | MEDLINE | ID: mdl-35287427

ABSTRACT

Stewart-Treves Syndrome is a rare and fatal condition where cutaneous angiosarcoma-a high-grade malignant tumor originating in the vascular and lymphatic endothelium-classically develops in the upper limbs post-mastectomy, with radiation therapy and axillary lymph node dissection. There are very few reports of the syndrome developing in the lower limbs, without any preceding malignancy or radiation therapy. The median development time is 11 years. Angiosarcoma originates in the vascular and lymphatic vessels, and the diagnosis is based on histopathology and immunohistochemistry findings. We report an unusual presentation of the Stewart-Treves Syndrome in an elderly female involving the lower limb with preexisting chronic lymphedema, where the tumor developed 15 months after total knee arthroplasty.

4.
J Cell Biol ; 220(9)2021 09 06.
Article in English | MEDLINE | ID: mdl-34347016

ABSTRACT

Cholesterol metabolism operates autonomously within the central nervous system (CNS), where the majority of cholesterol resides in myelin. We demonstrate that TDP-43, the pathological signature protein for amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), influences cholesterol metabolism in oligodendrocytes. TDP-43 binds directly to mRNA of SREBF2, the master transcription regulator for cholesterol metabolism, and multiple mRNAs encoding proteins responsible for cholesterol biosynthesis and uptake, including HMGCR, HMGCS1, and LDLR. TDP-43 depletion leads to reduced SREBF2 and LDLR expression, and cholesterol levels in vitro and in vivo. TDP-43-mediated changes in cholesterol levels can be restored by reintroducing SREBF2 or LDLR. Additionally, cholesterol supplementation rescues demyelination caused by TDP-43 deletion. Furthermore, oligodendrocytes harboring TDP-43 pathology from FTD patients show reduced HMGCR and HMGCS1, and coaggregation of LDLR and TDP-43. Collectively, our results indicate that TDP-43 plays a role in cholesterol homeostasis in oligodendrocytes, and cholesterol dysmetabolism may be implicated in TDP-43 proteinopathies-related diseases.


Subject(s)
Cholesterol/metabolism , DNA-Binding Proteins/genetics , Frontotemporal Dementia/genetics , Myelin Sheath/metabolism , Oligodendroglia/metabolism , Sterol Regulatory Element Binding Protein 2/genetics , Animals , DNA-Binding Proteins/deficiency , Disease Models, Animal , Female , Frontal Lobe/metabolism , Frontal Lobe/pathology , Frontotemporal Dementia/metabolism , Frontotemporal Dementia/pathology , Gene Expression Profiling , Gene Expression Regulation , Humans , Hydroxymethylglutaryl-CoA Synthase/genetics , Hydroxymethylglutaryl-CoA Synthase/metabolism , Lipid Metabolism/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Myelin Sheath/pathology , Oligodendroglia/pathology , Organoids/metabolism , Organoids/pathology , Primary Cell Culture , Receptors, LDL/genetics , Receptors, LDL/metabolism , Signal Transduction , Spinal Cord/metabolism , Spinal Cord/pathology , Sterol Regulatory Element Binding Protein 2/metabolism , Temporal Lobe/metabolism , Temporal Lobe/pathology
5.
Elife ; 102021 03 10.
Article in English | MEDLINE | ID: mdl-33689679

ABSTRACT

TDP-43 is extensively studied in neurons in physiological and pathological contexts. However, emerging evidence indicates that glial cells are also reliant on TDP-43 function. We demonstrate that deletion of TDP-43 in Schwann cells results in a dramatic delay in peripheral nerve conduction causing significant motor deficits in mice, which is directly attributed to the absence of paranodal axoglial junctions. By contrast, paranodes in the central nervous system are unaltered in oligodendrocytes lacking TDP-43. Mechanistically, TDP-43 binds directly to Neurofascin mRNA, encoding the cell adhesion molecule essential for paranode assembly and maintenance. Loss of TDP-43 triggers the retention of a previously unidentified cryptic exon, which targets Neurofascin mRNA for nonsense-mediated decay. Thus, TDP-43 is required for neurofascin expression, proper assembly and maintenance of paranodes, and rapid saltatory conduction. Our findings provide a framework and mechanism for how Schwann cell-autonomous dysfunction in nerve conduction is directly caused by TDP-43 loss-of-function.


Subject(s)
DNA-Binding Proteins/genetics , Exons , Intercellular Junctions/metabolism , Neural Conduction , Schwann Cells/metabolism , Animals , DNA-Binding Proteins/metabolism , Female , Male , Mice
6.
Acta Neuropathol Commun ; 9(1): 9, 2021 01 06.
Article in English | MEDLINE | ID: mdl-33407930

ABSTRACT

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) represent two ends of the same disease spectrum of adult-onset neurodegenerative diseases that affect the motor and cognitive functions, respectively. Multiple common genetic loci such as fused in sarcoma (FUS) have been identified to play a role in ALS and FTD etiology. Current studies indicate that FUS mutations incur gain-of-toxic functions to drive ALS pathogenesis. However, how the disease-linked mutations of FUS affect cognition remains elusive. Using a mouse model expressing an ALS-linked human FUS mutation (R514G-FUS) that mimics endogenous expression patterns, we found that FUS proteins showed an age-dependent accumulation of FUS proteins despite the downregulation of mouse FUS mRNA by the R514G-FUS protein during aging. Furthermore, these mice developed cognitive deficits accompanied by a reduction in spine density and long-term potentiation (LTP) within the hippocampus. At the physiological expression level, mutant FUS is distributed in the nucleus and cytosol without apparent FUS aggregates or nuclear envelope defects. Unbiased transcriptomic analysis revealed a deregulation of genes that cluster in pathways involved in nonsense-mediated decay, protein homeostasis, and mitochondrial functions. Furthermore, the use of in vivo functional imaging demonstrated widespread reduction in cortical volumes but enhanced functional connectivity between hippocampus, basal ganglia and neocortex in R514G-FUS mice. Hence, our findings suggest that disease-linked mutation in FUS may lead to changes in proteostasis and mitochondrial dysfunction that in turn affect brain structure and connectivity resulting in cognitive deficits.


Subject(s)
Amyotrophic Lateral Sclerosis/genetics , Brain/metabolism , Cognitive Dysfunction/genetics , Mitochondria/metabolism , Nonsense Mediated mRNA Decay/genetics , Proteostasis/genetics , Amyotrophic Lateral Sclerosis/metabolism , Amyotrophic Lateral Sclerosis/physiopathology , Animals , Brain/physiopathology , Cognitive Dysfunction/metabolism , Cognitive Dysfunction/physiopathology , Functional Neuroimaging , Humans , Magnetic Resonance Imaging , Mice , Mice, Transgenic , Morris Water Maze Test , Neural Pathways/metabolism , Neural Pathways/physiopathology , Open Field Test , RNA-Binding Protein FUS/genetics
7.
Proc Natl Acad Sci U S A ; 117(46): 29101-29112, 2020 11 17.
Article in English | MEDLINE | ID: mdl-33127758

ABSTRACT

Patients with amyotrophic lateral sclerosis (ALS) can have abnormal TDP-43 aggregates in the nucleus and cytosol of their surviving neurons and glia. Although accumulating evidence indicates that astroglial dysfunction contributes to motor neuron degeneration in ALS, the normal function of TDP-43 in astrocytes are largely unknown, and the role of astroglial TDP-43 loss to ALS pathobiology remains to be clarified. Herein, we show that TDP-43-deleted astrocytes exhibit a cell-autonomous increase in GFAP immunoreactivity without affecting astrocyte or microglia proliferation. At the transcriptomic level, TDP-43-deleted astrocytes resemble A1-reactive astrocytes and induce microglia to increase C1q expression. These astrocytic changes do not cause loss of motor neurons in the spinal cord or denervation at the neuromuscular junction. In contrast, there is a selective reduction of mature oligodendrocytes, but not oligodendrocyte precursor cells, suggesting triglial dysfunction mediated by TDP-43 loss in astrocytes. Moreover, mice with astroglial TDP-43 deletion develop motor, but not sensory, deficits. Taken together, our results demonstrate that TDP-43 is required to maintain the protective functions of astrocytes relevant to the development of motor deficits in mice.


Subject(s)
Astrocytes/metabolism , DNA-Binding Proteins/metabolism , Phenotype , Amyotrophic Lateral Sclerosis/genetics , Amyotrophic Lateral Sclerosis/metabolism , Animals , Cell Proliferation , DNA-Binding Proteins/genetics , Disease Models, Animal , Female , Gene Deletion , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Microglia/metabolism , Motor Neurons/metabolism , Neuromuscular Junction/metabolism , Oligodendroglia/metabolism , Transcriptome
8.
Dis Model Mech ; 12(10)2019 10 17.
Article in English | MEDLINE | ID: mdl-31515263

ABSTRACT

Tumors are frequently dependent on primary oncogenes to maintain their malignant properties (known as 'oncogene addiction'). We have previously established several inducible hepatocellular carcinoma (HCC) models in zebrafish by transgenic expression of an oncogene. These tumor models are strongly oncogene addicted, as the induced and histologically proven liver tumors regress after suppression of oncogene expression by removal of a chemical inducer. However, the question of whether the liver tumor cells are eliminated or revert to normal cells remains unanswered. In the present study, we generated a novel Cre/loxP transgenic zebrafish line, Tg(fabp10: loxP-EGFP-stop-loxP-DsRed; TRE: CreERT2) (abbreviated to CreER), in order to trace tumor cell lineage during tumor regression after crossing with the xmrk (activated EGFR homolog) oncogene transgenic line, Tg(fabp10: rtTA; TRE: xmrk; krt4: EGFP) We found that, during HCC regression, restored normal liver contained both reverted tumor hepatocytes (RFP+) and newly differentiated hepatocytes (GFP+). RNA sequencing (RNA-seq) analyses of the RFP+ and GFP+ hepatocyte populations after tumor regression confirmed the conversion of tumor cells to normal hepatocytes, as most of the genes and pathways that were deregulated in the tumor stages were found to have normal regulation in the tumor-reverted hepatocytes. Thus, our lineage-tracing studies demonstrated the potential for transformed tumor cells to revert to normal cells after suppression of expression of a primary oncogene. This observation may provide a basis for the development of a therapeutic approach targeting addicted oncogenes or oncogenic pathways.


Subject(s)
Hepatocytes/pathology , Liver Neoplasms/pathology , Oncogenes , Zebrafish/genetics , Animals , Animals, Genetically Modified , Apoptosis , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/pathology , Cell Differentiation , Cell Line, Transformed , Cell Lineage , Cell Proliferation , Gene Expression Regulation, Neoplastic , Hepatocytes/metabolism , Integrases/metabolism , Liver Neoplasms/genetics , Signal Transduction/genetics , Transcriptome/genetics
9.
Sci Rep ; 9(1): 1509, 2019 02 06.
Article in English | MEDLINE | ID: mdl-30728369

ABSTRACT

We have previously demonstrated the pro-tumoral role of neutrophils using a kras-induced zebrafish hepatocarcinogenesis model. To further illustrate the molecular basis of the pro-tumoral role, Tumor-associated neutrophils (TANs) were isolated by fluorescence-activated cell sorting (FACS) and transcriptomic analyses were carried out by RNA-Seq. Differentially expressed gene profiles of TANs from larvae, male and female livers indicate great variations during liver tumorigenesis, but the common responsive canonical pathways included an immune pathway (Acute Phase Response Signaling), a liver metabolism-related pathway (LXR/RXR Activation) and Thrombin Signaling. Consistent with the pro-tumoral role of TANs, gene module analysis identified a consistent down-regulation of Cytotoxicity module, which may allow continued proliferation of malignant cells. Gene Set Enrichment Analysis indicated up-regulation of several genes promoting angiogenesis. Consistent with this, we found decreased density of blood vessels accompanied with decreased oncogenic liver sizes in neutrophil-depleted larvae. Collectively, our study has indicated some molecular mechanisms of the pro-tumoral roles of TANs in hepatocarcinogenesis, including weakened immune clearance against tumor cells and enhanced function in angiogenesis.


Subject(s)
Carcinogenesis/genetics , Gene Expression Regulation, Neoplastic , Liver Neoplasms, Experimental/blood supply , Liver Neoplasms, Experimental/genetics , Neovascularization, Pathologic/genetics , Neutrophils/pathology , Transcriptome , Animals , Carcinogenesis/pathology , Gene Expression Profiling , Humans , Liver Neoplasms, Experimental/pathology , Mice , Neutrophils/metabolism , Signal Transduction , Zebrafish
10.
Adv Exp Med Biol ; 916: 147-68, 2016.
Article in English | MEDLINE | ID: mdl-27165353

ABSTRACT

The past decade has witnessed a remarkable advancement of the zebrafish model in cancer research. With the rapid development of genomic tools, it is increasingly feasible to perform genome-wide analyses to identify changes associated with cancer in a wide array of model organisms. These genomic tools, particularly transcriptomic analyses using DNA microarray and RNA sequencing platforms, have now become widely used in zebrafish cancer models to uncover novel biology and common molecular pathways underlying hepatocellular carcinoma, intrahepatic cholangiocarcinoma, melanoma, embryonal rhabdomyosarcoma (ERMS), T cell acute lymphoblastic leukemia (T-ALL), Ewing's sarcoma and glioma. An important finding from these studies is the high similarity and conservation of molecular pathways that underlie cancer in complementary zebrafish models and their human counterparts. Finally, these transcriptomic tools have also proven effective in the development and the validation of specific assays for chemical compound screening. In the future, other genomic tools, such as epigenetic, proteomic and metabolomic tools will likely be incorporated into zebrafish cancer studies, further refining our understanding of cancer.


Subject(s)
Disease Models, Animal , Gene Expression , Neoplasms/genetics , Transcriptome , Animals , Zebrafish
11.
Sci Rep ; 6: 19045, 2016 Jan 08.
Article in English | MEDLINE | ID: mdl-26743178

ABSTRACT

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and has adverse health effect on wildlife and humans. It has been implicated to have hepatotoxicity, but its molecular mechanisms remain unclear. In the present study, adult male zebrafish were exposed to TDCIPP and global hepatic gene expression was examined by RNA-Seq and RT-qPCR in order to understand the molecular mechanisms of TDCIPP-induced hepatotoxicity. Our results indicated that TDCIPP exposure significantly up-regulated the expression of genes involved in endoplasmic reticulum stress and Toll-like receptor (TLR) pathway, implying an inflammatory response, which was supported by up-regulation of inflammation-related biomaker genes. Hepatic inflammation was further confirmed by histological observation of increase of infiltrated neutrophils and direct observation of liver recruitment of neutrophils labeled with Ds-Red fluorescent protein of Tg(lysC:DsRed) zebrafish upon TDCIPP exposure. To further characterize the hepatotoxicity of TDCIPP, the expression of hepatotoxicity biomarker genes, liver histopathology and morphology were examined. The exposure to TDCIPP significantly up-regulated the expression of several biomarker genes for hepatotoxicity (gck, gsr and nqo1) and caused hepatic vacuolization and apoptosis as well as increase of the liver size. Collectively, our results suggest that exposure to TDCIPP induces hepatic inflammation and leads to hepatotoxicity in zebrafish.


Subject(s)
Chemical and Drug Induced Liver Injury/genetics , Flame Retardants/toxicity , Liver/drug effects , Organophosphorus Compounds/toxicity , Water Pollutants, Chemical/toxicity , Animals , Chemical and Drug Induced Liver Injury/metabolism , Chemical and Drug Induced Liver Injury/pathology , Embryo, Nonmammalian , Endoplasmic Reticulum Stress/drug effects , Gene Expression Regulation, Developmental , Genes, Reporter , Germinal Center Kinases , Inflammation , Larva/drug effects , Larva/growth & development , Larva/metabolism , Liver/growth & development , Liver/metabolism , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Male , NAD(P)H Dehydrogenase (Quinone)/genetics , NAD(P)H Dehydrogenase (Quinone)/metabolism , Neutrophil Infiltration/drug effects , Neutrophils/drug effects , Neutrophils/metabolism , Neutrophils/pathology , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Toll-Like Receptors/genetics , Toll-Like Receptors/metabolism , Zebrafish , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
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