The maternal effects of dietary restriction on Dnmt expression and reproduction in two clones of Daphnia pulex.

The inheritance of epigenetic marks induced by environmental variation in a previous generation is broadly accepted as a mediator of phenotypic plasticity. Transgenerational effects linking maternal experiences to changes in morphology, gene expression, and life history of successive generations are known across many taxa. While the number of studies linking epigenetic variation to ecological maternal effects is increasing rapidly, few if any attempts have been made to investigate molecular mechanisms governing epigenetic functions in the context of ecologically relevant maternal effects. Daphnia make an ideal model for investigating molecular epigenetic mechanisms and ecological maternal effects because they will reproduce asexually in the lab. Daphnia are also known to have strong maternal effects, involving a variety of traits and environmental variables. Using two clones of Daphnia pulex, we investigated the plasticity of life history and DNA methyltransferase (Dnmt) gene expression with respect to food limitation within and across generations. We found strong evidence of genotypic variation of responses of life history and Dnmt expression to low food diets, both within and across generations. In general, effects of offspring diet were larger than either the direct maternal effect or offspring-maternal environment interactions, but the direction of the maternal effect was usually in the opposite direction of the within-generation effect. For both life history and Dnmt expression, we also found that when offspring had low food, effects of the maternal environment were stronger than when offspring had high food.

Fitness effects of spontaneous mutations in a warming world.

Spontaneous mutations fuel evolutionary processes and differ in consequence, but the consequences depend on the environment. Biophysical considerations of protein thermostability predict that warm temperatures may systematically increase the deleteriousness of mutation. We sought to test whether mutation reduced fitness more when measured in an environment that reflected climate change projections for temperature. We investigated the effects of spontaneous mutations on life history, size, and fitness in 21 mutation accumulation lines and 12 control lines of Daphnia pulex at standard and elevated (+4℃) temperatures. Warmer temperature accelerated life history and reduced body length and clutch sizes. Mutation led to reduced mean clutch sizes and fitness estimates at both temperatures. We found no evidence of a systematic temperature-mutation interaction on trait means, although some lines showed evidence of beneficial mutation at one temperature and deleterious mutation at the other. However, trait variances are also influenced by mutation, and we observed increased variances due to mutation for most traits. For variance of the intrinsic rate of increase and some reproductive traits, we found significant temperature-mutation interactions, with a larger increase due to mutation in the warmer environment. This suggests that selection on new mutations will be more efficient at elevated temperatures.