Sodium Tungstate (NaW) Decreases Inflammation and Renal Fibrosis in Diabetic Nephropathy.

BACKGROUND: Diabetic Nephropathy is one of the most severe complications of Diabetes Mellitus and the main cause of end-stage kidney disease worldwide. Despite the therapies available to control blood glucose and blood pressure, many patients continue to suffer from progressive kidney damage. Chronic hyperglycemia is the main driver of changes observed in diabetes; however, it was recently discovered that inflammation and oxidative stress contribute to the development and progression of kidney damage. Therefore, it is important to search for new pharmacological therapies that stop the progression of DN. Sodium tungstate (NaW) is an effective short and long-term antidiabetic agent in both type 1 and type 2 diabetes models. METHODS: In this study, the effect of NaW on proinflammatory signalling pathways, proinflammatory proteins and fibrosis in the streptozotocin (STZ)-induced type 1 diabetic rat model was analysed using histological analysis, western blotting and immunohistochemistry. RESULTS: NaW treatment in diabetic rats normalize parameters such as glycemia, glucosuria, albuminuria/creatinuria, glomerular damage, and tubulointerstitial damage. NaW decreased the proinflammatory signaling pathway NF-κB, inflammatory markers (ICAM-1, MCP-1 and OPN), profibrotic pathways (TGFß1/Smad2/3), reduced epithelial-mesenchymal transition (α -SMA), and decreased renal fibrosis (type IV collagen). CONCLUSION: NaW could be an effective drug therapy for treating human diabetic nephropathy.

Discovery and Characterization of the ddx41 Gene in Atlantic Salmon: Evolutionary Implications, Structural Functions, and Innate Immune Responses to Piscirickettsia salmonis and Renibacterium salmoninarum Infections.

The innate immune response in Salmo salar, mediated by pattern recognition receptors (PRRs), is crucial for defending against pathogens. This study examined DDX41 protein functions as a cytosolic/nuclear sensor for cyclic dinucleotides, RNA, and DNA from invasive intracellular bacteria. The investigation determined the existence, conservation, and functional expression of the ddx41 gene in S. salar. In silico predictions and experimental validations identified a single ddx41 gene on chromosome 5 in S. salar, showing 83.92% homology with its human counterpart. Transcriptomic analysis in salmon head kidney confirmed gene transcriptional integrity. Proteomic identification through mass spectrometry characterized three unique peptides with 99.99% statistical confidence. Phylogenetic analysis demonstrated significant evolutionary conservation across species. Functional gene expression analysis in SHK-1 cells infected by Piscirickettsia salmonis and Renibacterium salmoninarum indicated significant upregulation of DDX41, correlated with increased proinflammatory cytokine levels and activation of irf3 and interferon signaling pathways. In vivo studies corroborated DDX41 activation in immune responses, particularly when S. salar was challenged with P. salmonis, underscoring its potential in enhancing disease resistance. This is the first study to identify the DDX41 pathway as a key component in S. salar innate immune response to invading pathogens, establishing a basis for future research in salmonid disease resistance.

Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia.

In the fall of 2022, decreased triclabendazole (TCBZ) efficacy against F. hepatica was suspected in a sheep farm located in the Santa Cruz province, Argentinian Patagonia. Since TCBZ-resistance in F. hepatica has never been reported in this province, this study aimed to confirm potential TCBZ-resistance in F. hepatica and to evaluate the efficacy of closantel (CLO) and nitroxinil (NTX), through faecal egg count reduction test (FECRT), and the efficacy of albendazole (ABZ) through the in vitro egg hatch test (EHT) in sheep. Sixty-eight (68) animals were selected from a herd of eighty (80) female Merino naturally infected with F. hepatica based on eggs per gram of F. hepatica (EPGFh) counts and assigned into four (4) groups (n = 17 per group): Group Control, animals did not receive anthelmintic treatment; Group TCBZ, animals were orally treated with TCBZ (12 mg/kg); Group CLO, animals were orally treated with CLO (10 mg/kg); and Group NTX, animals were subcutaneously treated with NTX (10 mg/kg). The fluke egg output was monitored on days 0 and 21 post-treatment. For the EHT, liver fluke eggs were isolated from faecal samples (approx. 50 g) collected from animals of the control group. TCBZ efficacy against liver fluke was 53.4%, confirming the presence of TCBZ-resistant isolates on the farm. CLO and NTX were highly effective (100%) for the treatment of F. hepatica on this farm. The EHT was carried out in two different laboratories, in which was observed an ABZ efficacy of 95.8 (Bariloche) and 96.5% (Tandil). These results indicate the ABZ susceptibility of this F. hepatica isolate and the inter-laboratory precision of the test.

Sodium Tungstate (NaW) Decreases Reactive Oxygen Species (ROS) Production in Cells: New Cellular Antioxidant.

Diabetic nephropathy (DN) is the leading cause of end-stage renal failure worldwide. Hyperglycemia generates reactive oxygen species (ROS), contributing to diabetic complications, especially in DN. Sodium Tungstate (NaW) is an effective antidiabetic agent for short and long-term treatments of both type 1 and type 2 diabetes models. In this study, we evaluated the effect of NaW on ROS production in bovine neutrophils incubated with platelet-activating factor (PAF) and in HK-2 cells induced by high glucose or H2O2. In addition, we evaluated the effect on iNOS expression in the type 1 diabetic rat model induced with streptozotocin (STZ). NaW inhibited ROS production in PAF-induced bovine neutrophils, and human tubular cells (HK-2) were incubated in high glucose or H2O2. In addition, NaW inhibited iNOS expression in glomeruli and tubular cells in the type 1 diabetic rat. This study demonstrates a new role for NaW as an active antioxidant and its potential use in treating DN.

Genomic and proteomic aspects of p57 protein from Renibacterium salmoninarum: Characteristics in virulence patterns.

Renibacterium salmoninarum is one of the oldest known fish bacterial pathogens. This Gram-positive bacterium is the causative agent of Bacterial Kidney Disease (BKD), a chronic infection that primarily infects salmonids at low temperatures. Externally, infected fish may show exophthalmos, skin blisters, ulcerations, and hemorrhages at the base of the fins and along the lateral line. Internally, the kidney, heart, spleen, and liver may show signs of inflammation. The best characterized virulence factor of R. salmoninarum is p57, a 57 kDa protein located on the bacterial cell surface and secreted into surrounding fish tissue. The p57 protein in fish is the main mediator in suppressing the immune system, reducing antibody production, and intervening in cytokine activity. In this review, we will discuss aspects such as single nucleotide polymorphisms (SNPs) that modify the DNA sequence, variants in the number of copies of MSA genes, physical-chemical properties of the signal peptides, and the limited iron conditions that can modify p57 expression and increase the virulence of R. salmoninarum.

Indole-diterpenes alkaloid profiles of native grasses involved in tremorgenic syndromes in the Argentine Patagonia.

One of the main intoxications to livestock in the Patagonia region of Argentina is the tremorgenic disease "Mal de Huecú", attributed to the consumption of the native grasses Poa huecu and/or Festuca argentina. In this report, five outbreaks of spontaneous intoxications were investigated. Several indole-diterpene alkaloids were identified in Poa huecu and Festuca argentina including the known tremorgen terpendole C and are likely the cause of "Mal de Huecú" disease.

Under pressure: phenotypic divergence and convergence associated with microhabitat adaptations in Triatominae.

BACKGROUND: Triatomine bugs, the vectors of Chagas disease, associate with vertebrate hosts in highly diverse ecotopes. It has been proposed that occupation of new microhabitats may trigger selection for distinct phenotypic variants in these blood-sucking bugs. Although understanding phenotypic variation is key to the study of adaptive evolution and central to phenotype-based taxonomy, the drivers of phenotypic change and diversity in triatomines remain poorly understood. METHODS/RESULTS: We combined a detailed phenotypic appraisal (including morphology and morphometrics) with mitochondrial cytb and nuclear ITS2 DNA sequence analyses to study Rhodnius ecuadoriensis populations from across the species' range. We found three major, naked-eye phenotypic variants. Southern-Andean bugs primarily from vertebrate-nest microhabitats (Ecuador/Peru) are typical, light-colored, small bugs with short heads/wings. Northern-Andean bugs from wet-forest palms (Ecuador) are dark, large bugs with long heads/wings. Finally, northern-lowland bugs primarily from dry-forest palms (Ecuador) are light-colored and medium-sized. Wing and (size-free) head shapes are similar across Ecuadorian populations, regardless of habitat or phenotype, but distinct in Peruvian bugs. Bayesian phylogenetic and multispecies-coalescent DNA sequence analyses strongly suggest that Ecuadorian and Peruvian populations are two independently evolving lineages, with little within-lineage phylogeographic structuring or differentiation. CONCLUSIONS: We report sharp naked-eye phenotypic divergence of genetically similar Ecuadorian R. ecuadoriensis (nest-dwelling southern-Andean vs palm-dwelling northern bugs; and palm-dwelling Andean vs lowland), and sharp naked-eye phenotypic similarity of typical, yet genetically distinct, southern-Andean bugs primarily from vertebrate-nest (but not palm) microhabitats. This remarkable phenotypic diversity within a single nominal species likely stems from microhabitat adaptations possibly involving predator-driven selection (yielding substrate-matching camouflage coloration) and a shift from palm-crown to vertebrate-nest microhabitats (yielding smaller bodies and shorter and stouter heads). These findings shed new light on the origins of phenotypic diversity in triatomines, warn against excess reliance on phenotype-based triatomine-bug taxonomy, and confirm the Triatominae as an informative model system for the study of phenotypic change under ecological pressure .

Detección prenatal de cardiopatías congénitas y factores relacionados. Experiencia en un hospital nacional / Prenatal detection of congenital cardiopathies and related factors. Experience in a national hospital

The authors present a estudy related to the prenatal detection of congenital heart diseases. The congenital anomalies (CA) are morphological or functional disorders of prenatal origin, present since the birth, even they can be detected later on, during their lifes. They constitute the second cause of infantile death in our country, following, prenatal diseases (prematurity, perinatal infections, etc.) The most severe CA have relevance for the health and require usually clinical a surgical treatment. Congenital cardiopaties (CC) are the most frequent and represent a great impact in the mobimortality both neonatal and pediatric. Foetal echocardiography is a sensitive and specific method of investigation for prenatal detection of cardiac malformations. Experience has shown that echocardiographic screening can demonstrate, the presence of probably various cardiac disease in the foetus as early as 18-20 th weeks of pregnancy. It is recommended that screening should be carried out as part of other forms of obstetric ultrasonic screening. Our aims were to analyze the characteristies of the pregnants at risk factors to CC. To determine the associated factors to the fetal pathology of the fetal electrocardiogram, and to correlate the postnatal diagnosis by cardiac echodoppler, which impoves neonate survival and reduces morbidity. The results obtained are detailed in the article (AU)

Intervenciones diagnósticas prenatales en un hospital público / Prenatal diagnostic interventions in a public hospital

The authors present a study of prenatal diagnostic interventions in a public hospital. The invasive prenatal diagnosis can be achieved by means of threee methods, aspirative punction of chorionic villous, extraction of amniotic fluid and fetal blood sample. Samples of chorionic villus are empoyed for citogenetic study, determination of the fetal cariotype or realization of a specific determination through technic FISH (Fluorescence in situ hybridization) or molecular genetics. Sample of amniotic fluid consists in the aspirative puncture under direct sonographic control of the amniotic cavity. During the period of the study, 9457 obstetric sonographies were performed with 121 genetic consultations of whom 46 resulted in invasive prenatal diagnostics. The results obtained are discussed (AU)

Comparison of exposure to trace elements through vegetable consumption between a mining area and an agricultural area in central Chile.

Human exposure to trace elements has been a large concern due to the potential health issues. Accordingly, this study aimed to compare the concentrations of arsenic, copper, and zinc in the edible parts of vegetables grown in a mining-agricultural area and in an exclusively agricultural area and to compare the potential human health risks of consuming vegetables from both areas. The consumption habits of the studied population were extracted from the 2010 National Alimentary Survey of Chile. In most cases, the concentrations of trace elements in the edible tissues of vegetables (lettuce, spinach, garlic, onion, carrot, potato, sweet corn, and tomato) were higher in the mining-agricultural area than those in the control area. This difference was most pronounced for leafy vegetables, with arsenic being the trace element of concern. Specifically, the arsenic concentrations in the edible tissues of lettuce and spinach were 8.2- and 5.4-fold higher, respectively, in the mining-agricultural area than in the control area. Lettuce was the vegetable of concern due to its relatively high consumption and relatively high concentration of trace elements. Nevertheless, there was no health risk associated with vegetable consumption in either the mining area or the control area because none of the HQ values surpassed 1.0.

Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling.

Increasing evidence indicates that the Golgi apparatus plays active roles in cancer, but a comprehensive understanding of its functions in the oncogenic transformation has not yet emerged. At the same time, the Golgi is becoming well recognized as a hub that integrates its functions of protein and lipid biosynthesis to signal transduction for cell proliferation and migration in cancer cells. Nevertheless, the active function of the Golgi apparatus in cancer cells has not been fully evaluated as a target for combined treatment. Here, we analyzed the effect of perturbing the Golgi apparatus on the sensitivity of the MDA-MB-231 breast cancer cell line to the drugs Actinomycin D and Vinblastine. We disrupted the function of ARF1, a protein necessary for the homeostasis of the Golgi apparatus. We found that the expression of the ARF1-Q71L mutant increased the sensitivity of MDA-MB-231 cells to both Actinomycin D and Vinblastine, resulting in decreased cell proliferation and cell migration, as well as in increased apoptosis. Likewise, the combined treatment of cells with Actinomycin D or Vinblastine and Brefeldin A or Golgicide A, two disrupting agents of the ARF1 function, resulted in similar effects on cell proliferation, cell migration and apoptosis. Interestingly, each combined treatment had distinct effects on ERK1/2 and AKT signaling, as indicated by the decreased levels of either phospho-ERK1/2 or phospho-AKT. Our results suggest that disruption of Golgi function could be used as a strategy for the sensitization of cancer cells to chemotherapy.

Nueva estrategias del manejo perinatal de las gastrosquisis en 78 casos consecutivos en un hospital público de alta complejidad / NEW STRATEGIES FOR THE PERINATAL MANAGEMENT OF GASTROSCHISIS IN 78 CONSECUTIVE CASES AT A HIGH COMPLEXITY PUBLIC HOSPITAL

Introducción: La gastrosquisis es un defecto congénito en la pared abdominal anterior, paraumbilical, generalmente derecho, de causa no clara. La prevalencia es de 1 a 5/ 10000 nacidos vivos, y va en aumento. Objetivo: Describir los resultados perinatales utilizando nuevas estrategias en el manejo de las gastrosquisis en el Hospital Nacional A. Posadas en un periodo de 3 años. Pacientes y métodos: Estudio observacional, descriptivo, retrospectivo. Se presentan 78 casos de gastrosquisis consecutivos durante 2012-2015. Se consideraron variables demográficas, antecedentes familiares, personales y obstétricos. Se registraron datos prenatales, del nacimiento, del defecto y evolución. Variables categóricas: frecuencias; Continuas: medianas/rangos. p valor < = 0.05 Resultados: La prevalencia de gastroquisis fue de 5/1000 nacimientos (78 casos), donde la mediana de la edad materna fue de 20 años y la mediana de la edad gestacional al momento del diagnostico fue de 21 semanas. Las imágenes patológicas observadas en ecografía fueron tamaño del defecto de pared, asa centinela y dilatación intrabdominal. Estos signos ecográficos no indican el tipo de resolución ni la evolución postnatal. En el 96 % de los casos se realizó cesárea segmentaria (el 41 % fue intraparto). La mediana de la edad gestacional al nacer fue de 36 semanas. En nuestro servicio la resolución de gastroquisis se realiza con: cierre inmediato, cierre diferido o colocación de silo. Solo hubo 5 muertes posnatales. Se observo que los neonatos con cierre inmediato comenzaron la prueba de tolerancia oral en menos tiempo que con silo o cierre diferido (p=0.03). Conclusión: Se propone la planificación del nacimiento lo más cercano al término independientemente de las imágenes ecográficas intestinales. El cierre postnatal inmediato reduciría la morbimortalidad y el tiempo de internación

Introduction: Gastroschisis is a paraumbilical abdominal anterior wall defect, generally on the right site , and with unclear origin. The prevalence is 1 to 5/10000 in live births, and is increasing. Objective: To describe the perinatal outcomes using new strategies in the management of gastroschisis in the Posadas Hospital over a period of 3 years. Patients and methods: observational, descriptive, retrospective study. 78 consecutive cases of gastroschisis are presented for the period 2012-2015. Demographic variables, family, personal and obstetric histories were considered. Prenatal data , birth defect and evolution were recorded. Categorical variables: frequency; Continuous: medium / ranges. p value <= 0.05. Results: The prevalence of gastroschisis was 5 in 1000 births (78 cases), the median maternal age was 20 years, and the median gestational age at diagnosis was 21 weeks. Size defect swall, Centinel asa, and intraabdominal dilatation were the pathological ultrasound images observed. These sonographic signs do not indicate the type of resolution or postnatal development. Cesarean section was performed in 96% of cases (41% was intrapartum). The median gestational age at birth was 36 weeks. Immediate closure, delayed closure or placement silo, were the gastroschisis resolutions in our Service. There were only 5 postnatal deaths. Infants submitted to immediate closure began tolerance oral intake in less time than with silo or delayed closure (p = 0.03). Conclusion: birth planning as close to term regardless of intestinal ultrasound images is proposed. The immediate postnatal closure would reduce morbidity and mortality and length of hospital stay

Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss).

Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields.

Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231.

Golgi phosphoprotein 3 (GOLPH3) has been implicated in the development of carcinomas in many human tissues, and is currently considered a bona fide oncoprotein. Importantly, several tumor types show overexpression of GOLPH3, which is associated with tumor progress and poor prognosis. However, the underlying molecular mechanisms that connect GOLPH3 function with tumorigenicity are poorly understood. Experimental evidence shows that depletion of GOLPH3 abolishes transformation and proliferation of tumor cells in GOLPH3-overexpressing cell lines. Conversely, GOLPH3 overexpression drives transformation of primary cell lines and enhances mouse xenograft tumor growth in vivo. This evidence suggests that overexpression of GOLPH3 could result in distinct features of GOLPH3 in tumor cells compared to that of non-tumorigenic cells. GOLPH3 is a peripheral membrane protein mostly localized at the trans-Golgi network, and its association with Golgi membranes depends on binding to phosphatidylinositol-4-phosphate. GOLPH3 is also contained in a large cytosolic pool that rapidly exchanges with Golgi-associated pools. GOLPH3 has also been observed associated with vesicles and tubules arising from the Golgi, as well as other cellular compartments, and hence it has been implicated in several membrane trafficking events. Whether these and other features are typical to all different types of cells is unknown. Moreover, it remains undetermined how GOLPH3 acts as an oncoprotein at the Golgi. Therefore, to better understand the roles of GOLPH3 in cancer cells, we sought to compare some of its biochemical and cellular properties in the human breast cancer cell lines MCF7 and MDA-MB-231 with that of the non-tumorigenic breast human cell line MCF 10A. We found unexpected differences that support the notion that in different cancer cells, overexpression of GOLPH3 functions in diverse fashions, which may influence specific tumorigenic phenotypes.

Cambio climático y salud en la región andina / Climate change and health in the andean region

Se presentan de manera resumida las causas fundamentales que contribuyen al calentamiento global y una serie de evidencias de la realidad que nos afecta: aumenta la temperatura de la tierra, se derriten los glaciares, sube el nivel de los océanos y se incrementa la frecuencia e intensidad de los eventos meteorológicos; todo ello como producto de la acumulación inusitada de gases de efecto invernadero, provenientes de la actividad humana. Se plantea las implicaciones que, de forma directa o indirecta, el cambio climático tiene para la salud, en particular para los países andinos: trastornos vinculados con la disponibilidad y calidad del agua y los alimentos, afecciones respiratorias, infecciones de transmisión vectorial, cáncer y enfermedades crónico degenerativas, cuadros asociados con desastres climáticos y temperaturas extremas. Finalmente, se revisa las propuestas y cursos de acción.

We present a short summary of the root causes that contribute to global warming and a host of evidence of the reality that affects us; such as: raising the temperature of the earth, melting glaciers, rising ocean level, increases the frequency and intensity of weather events, all as a result of the unusual accumulation of greenhouse gases, as product of human activity. There are implications that directly or indirectly, the climate change has to health in particular for Andean countries; such as: disorders linked to the availability and quality of water and food, respiratory disease, vector-borne infections, cancer and pathologies chronic degenerative tables associated with climatic disasters and extreme temperatures. Finally we review proposals and courses of action.

Neuronal activity of mitral-tufted cells in awake rats during passive and active odorant stimulation.

Odorants induce specific modulation of mitral/tufted (MT) cells' firing rate in the mammalian olfactory bulb (OB), inducing temporal patterns of neuronal discharge embedded in an oscillatory local field potential (LFP). While most studies have examined anesthetized animals, little is known about the firing rate and temporal patterns of OB single units and population activity in awake behaving mammals. We examined the firing rate and oscillatory activity of MT cells and LFP signals in behaving rats during two olfactory tasks: passive exposure (PE) and two-alternative (TA) choice discrimination. MT inhibitory responses are predominant in the TA task (76.5%), whereas MT excitatory responses predominate in the PE task (59.2%). Rhythmic discharge in the 12- to 100-Hz range was found in 79.0 and 68.9% of MT cells during PE and TA tasks, respectively. Most odorants presented in PE task increase rhythmic discharges at frequencies >50 Hz, whereas in TA, one of four odorants produced a modest increment <40 Hz. LFP oscillations were clearly modulated by odorants during the TA task, increasing their oscillatory power at frequencies centered at 20 Hz and decreasing power at frequencies >50 Hz. Our results indicate that firing rate responses of MT cells in awake animals are behaviorally modulated with inhibition being a prominent feature of this modulation. The occurrence of oscillatory patterns in single- and multiunitary discharge is also related to stimulation and behavioral context, while the oscillatory patterns of the neuronal population showed a strong dependence on odorant stimulation.

Trapping Triatominae in silvatic habitats.

Large-scale trials of a trapping system designed to collect silvatic Triatominae are reported. Live-baited adhesive traps were tested in various ecosystems and different triatomine habitats (arboreal and terrestrial). The trials were always successful, with a rate of positive habitats generally over 20% and reaching 48.4% for palm trees of the Amazon basin. Eleven species of Triatominae belonging to the three genera of public health importance (Triatoma, Rhodnius and Panstrongylus) were captured. This trapping system provides an effective way to detect the presence of triatomines in terrestrial and arboreal silvatic habitats and represents a promising tool for ecological studies. Various lines of research are contemplated to improve the performance of this trapping system.

Trapping Triatominae in silvatic habitats

Large-scale trials of a trapping system designed to collect silvatic Triatominae are reported. Live-baited adhesive traps were tested in various ecosystems and different triatomine habitats (arboreal and terrestrial). The trials were always successful, with a rate of positive habitats generally over 20 percent and reaching 48.4 percent for palm trees of the Amazon basin. Eleven species of Triatominae belonging to the three genera of public health importance (Triatoma, Rhodnius and Panstrongylus) were captured. This trapping system provides an effective way to detect the presence of triatomines in terrestrial and arboreal silvatic habitats and represents a promising tool for ecological studies. Various lines of research are contemplated to improve the performance of this trapping system