Local CD4+, CD8+ and CD56+ reactions to lung cancer in regard to pathohistological type and clinical stage.

AIM: To determinate the difference of abundance of CD4+, CD8+ and CD56+ bronchoalveolar fluid's lymphocytes and their subpopulations between non- and small cell lung cancer. Also, the differences of abundance of examined lymphocytes were compared between main clinical stages of lung cancer. METHODS: Mini-bronchoalveolar lavate was taken from lungs of 55 patients with cancer. After laboratory processing and adding CD3, CD4, CD8, CD27, CD28 and CD56 antibody, the material was analysed by flow cytometer. Results of Mini-BAL for non- and small cell lung cancer were compared, as well as the different clinical stages of the disease. RESULTS: Immature and regulatory forms of lymphocytes are more activated, while mature and activated forms are less activated in small cell lung cancer compared to non small type. With an increase of the clinical stage of disease, immunological reaction of T lymphocytes is better expressed because of increasing of abundance of immature and regulatory forms of different subpopulations of lymphocytes. CONCLUSION: All components of local CD4+ and CD8+ T lymphocyte, as well as NK and NKT cells response were more activated in lungs with small cell lung cancer, and these reactions were more expressed with an increase in the clinical stage.

Local CD4+, CD8+ and CD56+ T-lymphocite Reaction on Primary Lung Cancer.

OBJECTIVE: The primary goal of this study was to determine the difference of abundance of CD4+, CD8+ and CD56+ bronchoalveolar fluid's lymphocytes and their subpopulations between cancerous lung and healthy lung from the same patient. METHODS: Mini-bronchoalveolar lavage was taken from 55 patients from lung with cancer and healthy lung. After laboratory processing and addition of CD4, CD8, CD27, CD28 and CD56 antibody, the material was analyzed by flow cytometer. Results from lung with cancer were compared to the ones from the healthy lung. The examined patients were the test and the control group at the same time. RESULTS: CD27+28+ forms of CD4+ and CD8+ lymphocytes are more activated in the cancerous lung compared to healthy lung, while the CD27-28- forms are less activated in diseased lung. CD4+ forms of CD56+ lymphocytes are more activated in cancerous lung compared to the health lung, while the CD8+ forms are less activated in diseased lung. CONCLUSION: Immature helper and cytotoxic T lymphocyte response, as well as regulatory NK and NKT cell response are more activated in cancerous lung compared to the health lung of the same patient.

[Seroconversion makers of hepatitis in the dialysis center at the General Hospital in Tesanj (10 years' experience)]. / Serokonverzija markera hepatitisa u dijaliznom centru Opce bolnice u Tesnju (desetogodisnje iskustvo).

In the paper are stated the data of ten years observations of the occurrences of the seroconversion in the patients at the dyalized treatment in the dyalized center in Tesanj. The seropositiveness to B and C hepatitis was 16.5%, what is the usual percentage for the local population. The serconversion to the positive C hepatitis occurred in one patient, and that in the first year of the dialyzed treatment, what remains the possibility the dyalizis began in the period of the incubation. The separation of the seropositive apparati for the dyalisisi from the rest made the sufficient organizational measure by the dyalized center.