ABSTRACT
Sperm cryopreservation is a cost-effective means of preserving gene resources and transporting sperm across distant locations. However, due to the general disadvantages of using freeze-thawed sperm, to prevent this irreversible damage, cryopreservation techniques must be improved by the addition of additional cryoprotection agents. This study aims to improve the freezability of buck semen using an intratesticular injection of Autologous Platelet-Rich Plasma ( PRP) and confirm this theory by Casa laboratory analysis and gene expression detection of three genes (CATSPER1 , SPAG5 and Hsp70). Twenty rabbits a New Zealand healthy male were randomly divided into two equal groups; the control and PRP group which enriched with PRP, the semen collection was applied after 10 weeks, then each sample was divided into two fractions; First fractions we apply the laboratory semen analysis directly, and the second fractions were cryopreservation, then thawed after one month to apply the same laboratory analysis. The results of CASA, DNA fragmentation, and real time-PCR analysis had statistically significant differences (P<0.01) when compartment of these values after and before freezing, yet we don't record any statistical differences between the C group and CR group. This study's findings are extremely significant, indicating that intratesticular injection of PRP is a good method for using in enhancing the rabbit sperm procedure after freeze-thawing.