ABSTRACT
In this study, ß- glucan was extracted by the hot water extraction method followed by ethanol precipitation and purified using ion and gel filtration chromatography, then evaluate the anticancer effects of ß- glucan that purified from Phoenix dactylifera on cancer cell line. Ahmed Nahi Glioblastoma Multiform (ANGM) cancer cell line was used in the in vitro study. Cell line exposure times were calculated after 24, 48, and 72 hours in a micro titration plate under absolutely sterile conditions. High molecular weight ß-glucans can be obtained using the hot water extraction method without having to use strong agents to change their structure, like alkalis or acids. Anti-cancer property of ß-glucan derived from Phoenix dactylifera fruits on cancer cell lines has been reported. In this work, the ANGM cell line was treated with different concentrations of ß-glucan (31.25, 62.5, 125, 250, 500 and 1000 µg/mL). and the inhibition of the cells was investigated using the MTT assay after 24, 48 and 72 hours. The result obtained showed time and concentration dependent cytotoxic effect, and the higher concentrations at 48 hrs of exposure gave significantly (p<0.05) higher cytotoxic effect.