An Absorbance Peak Template for Clinical Assessment of Sound Conduction in Newborn Ears.

PURPOSE: Power absorbance measures recorded over a wide range of frequencies allow for clinical inferences about the outer/middle ears' acoustic mechanics. A frequency-dependent feature in the newborn wideband absorbance response, the prominent mid-frequency absorbance peak, has been linked to middle-ear resonance. However, current normative methods were not designed to assess subtle changes in such features. This work aims to develop and validate an absorbance peak template (APT) for assessment of absorbance peaks in newborns. Additional objectives are to compare test performance of absorbance peaks and APTs to existing normative methods, to demonstrate APT-based methods for categorization of abnormal absorbance peaks, and to describe absorbance peak test-retest variability. METHOD: Peak absorbance and peak frequency were analyzed in a training data set (490 measurements in 84 newborn ears who passed transient evoked otoacoustic emissions [TEOAEs] screenings), and an APT was developed by computing normal limits on these two absorbance peak variables. Split-set analysis evaluated the reproducibility of APT, and test-retest analysis was performed. Test performance analysis, conveyed by area under the receiver operating characteristic curve (AROC) and 95% confidence intervals (CIs), compared absorbance peak variables to absorbance area indices (AAIs) in a validation data set (359 ears that passed distortion-product OAE [DPOAE] screening and 64 ears that failed). APT-based assessment paradigms for normal and abnormal ears were compared to the common absorbance normative range paradigm. RESULTS: Split-set analysis demonstrated a good reproducibility of APT, and test-retest of absorbance peak variables showed that they were stable measures for clinical assessment. Test performance of peak absorbance (AROC = 0.83; 95% CI [0.77, 0.88]) was comparable to the top-performing AAI variables (AROC = 0.85; 95% CI [0.80, 0.90]). APT-based assessment categorized measurements based on their peak absorbance and peak frequency and enhanced the detection of subtle frequency changes that were missed by the normative range method. CONCLUSION: Analysis of absorbance peaks guided by APT has the potential to simplify and improve assessments of sound conduction pathways in newborn ears and can be used together with or in-place of current methods for analysis of wideband absorbance data.

Refining Measurements of Power Absorbance in Newborns: Probe Fit and Intrasubject Variability.

OBJECTIVES: Because unresolved debris in the ear canal or middle ear of newborns may produce high false positive rates on hearing screening tests, it has been suggested that an outer/middle ear measure can be included at the time of hearing screening. A potential measure is power absorbance (absorbance), which indicates the proportion of power in a broadband acoustic stimulus that is absorbed through the outer/middle ear. Although absorbance is sensitive to outer/middle dysfunction at birth, there is large variability that limits its accuracy. Acoustic leaks caused by poor probe fitting further exacerbate this issue. The objectives of this work were to: (1) develop criteria to indicate whether a change in absorbance occurs in association with probe fit; (2) describe the variability in absorbance due to poor fitting; and (3) evaluate test-retest variability with probe reinsertions, excluding poor fits. DESIGN: An observational cross-sectional design was used to evaluate changes in absorbance due to probe fit and probe reinsertion. Repeated measurements were recorded in 50 newborns (98 ears) who passed TEOAE screenings and were <48 hours of age. One absorbance measurement was chosen as the baseline that served as a best-fit reference in each ear. Changes in absorbance, called absorbance probe-fit Δ, were calculated relative to the baseline in each ear. Correlations were assessed between the absorbance probe-fit Δ and low-frequency absorbance, impedance magnitude, impedance phase, and equivalent volume, to determine which measures predicted poor fits. Criteria were derived from the strongest of these correlations and their performance was analyzed. Next, measurements with poor/leaky fits were identified, and the changes in absorbance that they introduced were analyzed. Excluding the poor fits, test-retest differences in absorbance, called reinsertion Δ, were determined. Variability was assessed using the SDs associated with absorbance, absorbance probe-fit Δ, and reinsertion Δ. RESULTS: Based on the analysis of 12 moderate-strong correlations, the following criteria were adopted to identify measurements with poor fits: (1) impedance phase-based criterion (500 to 1000 Hz) > -0.11 cycles and (2) absorbance-based criterion (250 to 1000 Hz) > 0.58. Poor-fit measurements introduced statistically significant increases in absorbance up to 0.1 for 1000 to 6000 Hz, and up to 0.4 for frequencies <1000 Hz. Reinsertion Δ were ≤0.02, and were significant for 500 to 5000 Hz. The SDs of absorbance probe-fit Δ were greatest and similar to overall absorbance SD in the low frequencies. Separately, the SDs of reinsertion Δ were also greatest and similar to low-frequency absorbance SD. CONCLUSIONS: Poor probe fits introduced the greatest inflation in absorbance for frequencies < 500 Hz, and a smaller but significant inflation for higher frequencies, consistent with controlled experiments on acoustic leaks in adults. Importantly, inflation of absorbance in diagnostically sensitive 1000 to 2000 Hz may impact its clinical performance. Test-retest with probe reinsertion contributed significantly to absorbance variability, especially in the low frequencies, consistent with reports in adults, even though changes were smaller than those associated with poor probe fit. The results indicate that variability in absorbance was reduced by minimizing acoustic leaks. Pending further validation, the probe-fit criteria developed in this work can be recommended to ensure proper probe fit.

Frequency-change in DPOAE evoked by 1 s/octave sweeping primaries in newborns and adults.

Distortion product otoacoustic emissions (DPOAE) in newborns and adults were evoked by sweeping primaries up and down in frequency at 1 s/octave. Sweeping up and down in frequency resulted in changes in the amplitude vs. frequency functions of the composite DPOAE and its two major components. In addition, DPOAE component phases differed slightly between the up- and down-swept conditions. The changes in amplitude vs. frequency functions were quantified using a covariate correlation technique, yielding single-valued estimates of the magnitude of the frequency changes. Separate analyses were performed for the entire DPOAE frequency range and split into low and high frequency ranges. There were consistent changes in newborn and adult composite DPOAEs and reflection components, but not generator components. Adults had significant frequency changes in the composite DPOAE for all frequency ranges and in the reflection component for the entire frequency range. Newborns had significant frequency change in the reflection component for all frequency ranges. Differences in frequency change between adults and newborns may stem from developmental changes in cochlear processing. Alignment of the component phase differences between the up- and down-swept conditions resulted in elimination of frequency-change in reconstructed composite DPOAEs.